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141.
Sequence variation of partial cytochrome b genes between two Coilia species, C. ectenes and C. mystus, was investigated. Of the 402 nucleotides, twenty-seven (6.72%) are polymorphic and all are synonymous substitutions. At
the third positions of genetic condon of cytochrome b gene, the two species show an extreme anti-G bias (<4%) and a pronounced bias towards A and C (>68%). There is no amino acid
sequence divergence between the partial cytochrome b genes of the two species, indicating a close genetic relationship between them. The k-2p genetic distance of partial cytochrome
b segment of the two species is 0.072, suggesting that the species were separated 3.6 Ma ago, in the middle Pliocene. Our result
reveals that the cytochrome b gene is an appropriate marker for studies of population genetic structures and phylogeographic patterns of the two species. 相似文献
142.
Combined effects of cadmium and butachlor on soil enzyme activities and microbial community structure 总被引:5,自引:0,他引:5
The combined effects of cadmium (Cd, 10 mg/kg of soil) and butachlor (5, 10 and 50 mg/kg of soil) on enzyme activities and
microbial community structure were assessed in phaeozem soil. The result showed that phosphatase activities were decreased
in soils with Cd (10 mg/kg of soil) alone whereas urease acitivities were unaffected by Cd. Urease and phosphatase activities
were significantly reduced by high butachlor concentration (50 mg/kg of soil). When Cd and butachlor concentrations in soils
were added at milligram ratio of 2:1 or 1:2, urease and phosphatase activities were decreased, while enzyme activities were
greatly improved at the ratio of 1:5. This study indicates that the combined effects of Cd and butachlor on soil urease and
phosphatase activities depend largely on the addition concentration ratios to soils. The random amplified polymorphic DNA
(RAPD) analysis showed that the changes occurring in RAPD profiles of different treated samples included variation in loss
of normal bands and appearance of new bands compared with the control soil. The RAPD fingerprints showed substantial differences
between the control and treated soil samples, with apparent changes in the number and size of amplified DNA fragments. The
results showed that the addition of high concentration butachlor and the combined applied Cd and butachlor significantly affected
the diversity of microbial community. The present results suggest that RAPD analysis in conjunction with other biomarkers
such as soil enzyme parameter etc. would prove a powerful ecotoxicological tool. 相似文献
143.
采用RAPD技术分析了湛江沿海野生蜂巢石斑鱼的遗传多样性 ,从 6个系列 1 2 0个引物筛选出1 6个引物 ,对 1 0尾蜂巢石斑鱼进行RAPD分析 ,1 6个引物共检测到 1 5 4个位点 ,其中 ,多态位点比例 (P)为 6 5 5 8% ,平均个体间遗传相似系数 (S)为 0 7887,平均个体间遗传距离 (D)为 0 2 1 1 3,遗传多样性指数 (H)为 0 1 776。研究结果表明 ,湛江沿海的野生蜂巢石斑鱼的遗传多样性较高。 相似文献
144.
We calculated the ionization fraction for hydrogen and helium (He I) as a function of the redshift z by including the two-photon decays of high hydrogen and parahelium levels and the radiative transfer in the helium 23P1 ? 11S0 intercombination line. We show that this yields corrections of no more than a few percent to the ionization fraction for hydrogen and speeds up significantly the recombination for helium compared to the recent works by Seager et al. (1999, 2000), in which these effects were disregarded. 相似文献
145.
146.
I~ODWrIONExtraction of DNA from Porphyra po~nsis is very difficult due tO its high content ofpolysaccharides. TO avoid the interference Of POlysaccharides, HOng et al. (1992) used LiCI tosoften Porphyra thalli cells to release DNA directly without lysing the thalli, hoWever, the procedure is problematic in the isolation of high-quality DNA. Kitade et al. (1996) established a complicated procedure which includes the grindiflg of material in liquid nitrogen, lysing of material inextra… 相似文献
147.
148.
固定标本的DNA提取及PCR扩增 总被引:4,自引:1,他引:3
报道福尔马林、酒精固定的日本绒螯蟹标本的 DNA提取及 PCR扩增 ,并与冷冻、煮沸标本进行了比较。结果表明 ,福尔马林固定标本与酒精固定标本一样可以提取 DNA,加入的蛋白酶 K量越多 ,DNA的回收率越高。以提取的 DNA为模板 ,进行了线粒体 DNA12 S r RNA和 D- loop基因片段的 PCR扩增 ,经琼脂糖凝胶电泳 PCR扩增产物后均得到了与期待的碱基长度相一致的清晰谱带。 相似文献
149.
150.
建立螺旋藻转基因体系初报 总被引:9,自引:1,他引:8
Mao Yunxiang 《中国海洋大学学报(自然科学版)》2000,30(Z1):13-18
从钝顶螺旋藻单细胞克隆的制备、重组平台的 克隆、同源重组表达质粒的构建、质粒的电激转化和报告基因的表达等方面对螺旋藻转基因 表达系统进行了研究,初步建立起螺旋藻转基因体系。用次氯酸钠溶液处理获得无菌培养系 ;用钠、钙离子混合液处理,获得了可再生的螺旋藻单细胞。用含EDTA的培养基预培养和用 培养基洗涤可分别去除胞外核酸酶活性和抑制胞内核酸酶活性。以氯霉素乙酰转移酶基因替 换大肠杆菌表达质粒pBV220的抗性选择标记,同时插入系列同源重组片段和萤火虫荧光素酶 基因,构建了同源重组表达载体pBVCS01-04L。电激转化螺旋藻S6-4品系单 细胞,获得了具有稳定氯霉素抗性的培养系,并用SDS-聚丙烯酰胺凝胶电泳证实了报告基 因的表达。 相似文献