环介导等温扩增联合横向流动试纸条快速检测扁浒苔(Ulva compressa)的研究   总被引：2，自引：1，他引：1  

陈先锋  周前进  王瑞娜  段维军  苗亮  陈炯 《海洋与湖沼》2015,46(4):819-827

本研究将环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)与横向流动试纸条(lateral flow dipstick,LFD)的可视化检测方法结合,建立了扁浒苔(Ulva compressa)的LAMPLFD快速检测技术。该方法以扁浒苔的内转录间隔区(ITS1-5.8S-ITS2)序列为检测靶标,设计了3对特异性引物(其中,上游内引物由生物素标记)和1条异硫氰酸荧光素标记的探针。结果表明,LAMP最适反应温度为63°C,扩增时间为60 min,从核酸扩增到LFD结果判读需70 min。利用LAMP-LFD可特异性检出扁浒苔,对浒苔、曲浒苔、缘管浒苔和孔石莼等石莼属绿藻以及塔玛亚历山大藻、无纹环沟藻、东海原甲藻、锥状斯克里普藻和赤潮异弯藻等常见微藻的检测结果为阴性。该方法最低可检测到0.1 pg的扁浒苔基因组DNA,是以Uco ITS-F3和Uco ITS-B3为特异性引物的PCR方法的100倍。对实际样品的检测结果表明,LAMP-LFD方法检测扁浒苔与传统的形态学观察的结果一致。因此,该方法可快速、特异地检测出扁浒苔,而且操作简单,仪器设备依赖性低,有潜力成为扁浒苔现场检测的常规技术手段。  相似文献   

A creep model for compacted loess based on 1D oedometer testEI北大核心CSCD     

Ge Miao-miao  Li Ning  Zheng Jian-guo  Zhang Ji-wen  Zhu Cai-hui 《岩土力学》2015,(11):3164-+

A series of long-term 1D oedometer test on compacted loess has been conducted using a high pressure consolidometer. It is found that the compacted loess has remarkable creep deformation and the proportion of the creep deformation in total deformation can come up to 6%-23%. The creep deformation proportion goes higher as the water content of the sample increases and the compaction degree reduces. And the creep deformation proportion decreases with the increase of stress level. A nonlinear creep model, which can well describe the rules of post-construction settlement of compacted loess, is proposed. The validity of model is verified by comparing the results from proposed model with those from the layer-wise summation method. The proposed model is employed to examine the variation law of the post-construction settlement under the conditions of the different water contents and compaction degrees. It is found that there exist logarithmic relationships between the post-construction settlement and the compaction degree/water contents. Supposing the velocity of the post-construction settlement v<0.1 mm/d is the stabilized standard, the high embankment settlement trends to be stable in 200 to 650 days after construction. The higher the compaction degree is, the lower the water content is, the longer the time up to stable required is.  相似文献   

PEPSI: The high‐resolution échelle spectrograph and polarimeter for the Large Binocular Telescope     

K.G. Strassmeier  I. Ilyin  A. Jrvinen  M. Weber  M. Woche  S. I. Barnes  S.‐M. Bauer  E. Beckert  W. Bittner  R. Bredthauer  T. A. Carroll  C. Denker  F. Dionies  I. DiVarano  D. Dscher  T. Fechner  D. Feuerstein  T. Granzer  T. Hahn  G. Harnisch  A. Hofmann  M. Lesser  J. Paschke  S. Pankratow  V. Plank  D. Plüschke  E. Popow  D. Sablowski  J. Storm 《Astronomische Nachrichten》2015,336(4):324-361

PEPSI is the bench‐mounted, two‐arm, fibre‐fed and stabilized Potsdam Echelle Polarimetric and Spectroscopic Instrument for the 2×8.4 m Large Binocular Telescope (LBT). Three spectral resolutions of either 43 000, 120 000 or 270 000 can cover the entire optical/red wavelength range from 383 to 907 nm in three exposures. Two 10.3k×10.3k CCDs with 9‐µm pixels and peak quantum efficiencies of 94–96 % record a total of 92 échelle orders. We introduce a new variant of a wave‐guide image slicer with 3, 5, and 7 slices and peak efficiencies between 92–96 %. A total of six cross dispersers cover the six wavelength settings of the spectrograph, two of them always simultaneously. These are made of a VPH‐grating sandwiched by two prisms. The peak efficiency of the system, including the telescope, is 15 % at 650 nm, and still 11 % and 10 % at 390 nm and 900 nm, respectively. In combination with the 110 m² light‐collecting capability of the LBT, we expect a limiting magnitude of ≈20th mag in V in the low‐resolution mode. The R = 120 000 mode can also be used with two, dual‐beam Stokes IQUV polarimeters. The 270 000‐mode is made possible with the 7‐slice image slicer and a 100‐µm fibre through a projected sky aperture of 0.74″, comparable to the median seeing of the LBT site. The 43 000‐mode with 12‐pixel sampling per resolution element is our bad seeing or faint‐object mode. Any of the three resolution modes can either be used with sky fibers for simultaneous sky exposures or with light from a stabilized Fabry‐Pérot étalon for ultra‐precise radial velocities. CCD‐image processing is performed with the dedicated data‐reduction and analysis package PEPSI‐S4S. Its full error propagation through all image‐processing steps allows an adaptive selection of parameters by using statistical inferences and robust estimators. A solar feed makes use of PEPSI during day time and a 500‐m feed from the 1.8 m VATT can be used when the LBT is busy otherwise. In this paper, we present the basic instrument design, its realization, and its characteristics. Some pre‐commissioning first‐light spectra shall demonstrate the basic functionality. (© 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)  相似文献   

资源一号02C卫星数据在北京岩溶水资源勘查评价工程中的应用   总被引：2，自引：0，他引：2  

程洋  童立强  郭兆成  莫源富  纪轶群 《国土资源遥感》2015,(2):183-189

以资源一号02C(ZY1-02C)卫星数据为数据源,以GIS为数据处理平台,以遥感地质理论和水文地质理论为指导,将遥感技术应用于北京岩溶水资源勘查评价工程中。通过水文地质遥感解译和综合分析,优选了一处新的供水水源地,建立了适用于北方岩溶区的含水岩组和泉点的遥感解译标志,提出了基于GIS的水文地质分析方法,扩展了国产资源卫星数据的应用领域。  相似文献   

基于MODIS数据的湖北省油菜种植分布信息提取   总被引：1，自引：0，他引：1  

王凯  张佳华 《国土资源遥感》2015,(3)

MODIS归一化差值植被指数(MODIS-normalized difference vegetation index,MODIS-NDVI)时间序列产品能够连续反映植被的覆盖情况,是农作物遥感测量的重要数据源。为研究基于MODIS数据的油菜种植分布信息提取技术,选取湖北省为研究区,利用2008—2013年75个时相的MODIS-NDVI时序数据,结合农作物物候和地面调查样本等辅助资料,通过建立油菜种植面积提取模型,采用多次阈值比较方法提取了2009—2013年湖北省油菜种植分布信息,与统计数据比较,总体提取精度为85%左右。最后利用环境小卫星HJ-1A CCD数据进行精度验证,证明了MODIS-NDVI时序数据及本文方法在油菜种植面积提取中的可靠性,对掌握油菜种植面积和产量信息、加强农业生产管理、调整农业结构及辅助政府有关部门制定科学合理的农业政策具有重要意义。  相似文献   

基于扫描统计的甲型H1N1流感聚集性分析     

姚欣  夏天琦  翁敏 《测绘工程》2015,(10):56-58

扫描统计已被广泛应用于地域性疾病的聚集性检测,且可检测这种聚集的差异显著性。文中使用扫描统计的方法,通过对2009~2012年全国各省的甲型H1N1流感数据进行时空扫描以及逐年的空间扫描,生成高发病率地区和低发病率地区聚类,并通过叠置分析反映各个地区归入聚类的频次。利用专题地图和统计表分析甲型流感在2009~2012年中的爆发情况和趋势,并对结果进行客观的分析。  相似文献   

利用暗目标法从高分一号卫星16 m相机数据反演气溶胶光学厚度   总被引：5，自引：3，他引：2  

王中挺  辛金元  贾松林  厉青  陈良富  赵少华 《遥感学报》2015,19(3):530-538

针对高分一号卫星(GF-1)的16 m宽覆盖相机数据,探讨了暗目标法的应用。首先,利用地面观测的植被光谱数据,结合模拟计算,发现利用红蓝波段线性关系能更好地去除地表影响,而利用反演的气溶胶光学厚度AOD进行大气校正能很好地去除伪暗目标;然后,以天津地区和北京地区为试验区进行了反演试验。结果表明,利用本算法能较好地观测气溶胶分布,与地面观测结果均有较好的相关性(R0.8),但反演结果整体偏高,可能是云像元的影响。误差分析表明,整景图像采用统一的观测天顶角会带来较大误差,最大误差为0.3;绝对辐射定标精度在3%以下,反演精度能控制在10%,城市型气溶胶会对反演带来较大误差。  相似文献   

“天绘一号”卫星三线阵CCD影像自检校区域网平差     

张艳  王涛  冯伍法  元朝鹏  王淑香 《遥感学报》2015,19(2):219-227

遥感卫星在空间环境运转过程中,星敏感器的姿态角、成像相机的镜头和CCD等几何参数会发生不可预估的变化,从而对卫星影像的定位精度产生影响。因此,对卫星影像进行自检校区域网平差处理是实现卫星影像精确定位的一项关键技术。本文以"天绘一号"卫星为研究对象,首先分析了"天绘一号"卫星三线阵立体测绘相机的镜头和CCD几何形变,并提出了适用于"天绘一号"卫星三线阵CCD影像的自检校模型;然后建立"天绘一号"卫星三线阵CCD影像的自检校区域网平差模型,对外方位元素和自检校参数进行整体平差,消除存在于外方位元素观测值和自检校标定值中的系统误差,以提高卫星图像定位精度。最后利用嵩山实验场对"天绘一号"卫星三线阵CCD影像进行了自检校区域网平差处理,验证了自检校模型和自检校区域网平差模型的正确性和有效性,并分析研究了不同数目的控制点条件对自检校区域网平差的影响。实验结果证实采用自检校区域网平差技术可以有效地消除系统定位误差,显著提高定位精度。  相似文献   

利用空间实测数据进行板块构造分析     

田亮  刘武凤  姜鹏远  孙建军 《测绘科学》2015,(12):149-152

针对全球台站数量剧增、空间定位测量精度越发提高的情况,该文利用ITRF2008框架GPS、卫星激光测距、甚长基线干涉测量实测站速度信息,北美、中国以及欧洲连续运行卫星定位网络站速度信息进行基准统一,联合解算国际地球自转服务协议中14个主要大陆板块的欧拉矢量,对原有的板块运动参数进行精化;提出最小二乘海量台站选取方法。实验结果表明,该台站选取方法能够有效提高海量台站的筛选效率和可靠性;稳定台站的数量越多,分布越均匀,越能够体现板块运动的现势性。  相似文献   

地震波面波空间相干性规律研究     

朱园园  丁海平 《震灾防御技术》2021,16(3):467-475

In this paper, seismic records of Taiwan LSST array and SMART-1 array were selected to calculate the S-wave and surface wave coherence coefficients at different station distances. And then the coherence function model proposed by Loh was used to fit the calculation results. After comparison and analysis, we found that when the distance d < 50 m, the coherency coefficients of surface wave and S-waves are basically the same; when the distance d = 50 m , the coherency coefficients of surface wave is smaller than that of S-wave, and as the distance increases, the differences gradually increase. When the distance d > 500 m, the spatial coherency of the surface wave hardly exists, so no further consideration is needed. Finally, the surface wave coherency model parameters were given in this paper, which can be used as a reference for the synthetic ground motion field in the seismic analysis for long and large structures in large basins.  相似文献