Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay     

Taban IC  Bechmann RK  Torgrimsen S  Baussant T  Sanni S 《Marine environmental research》2004,58(2-5):701-705

The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins (Strongylocentrotus droebachiensis) and mussels (Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination.  相似文献   

Circulation and currents in the southwestern East/Japan Sea: Overview and review     

K.-I. Chang  W.J. Teague  S.J. Lyu  H.T. Perkins  D.-K. Lee  D.R. Watts  Y.-B. Kim  D.A. Mitchell  C.M. Lee  K. Kim 《Progress in Oceanography》2004,61(2-4):105

A review is made of circulation and currents in the southwestern East/Japan Sea (the Ulleung Basin), and the Korea/Tsushima Strait which is a unique conduit for surface inflow into the Ulleung Basin. The review particularly concentrates on describing some preliminary results from recent extensive measurements made after 1996. Mean flow patterns are different in the upstream and downstream regions of the Korea/Tsushima Strait. A high velocity core occurs in the mid-section in the upstream region, and splits into two cores hugging the coasts of Korea and Japan, the downstream region, after passing around Tsushima Island located in the middle of the strait. Four-year mean transport into the East/Japan Sea through the Korea/Tsushima Strait based on submarine cable data calibrated by direct observations is 2.4 Sv (1 Sv = 10⁶ m³ s⁻¹). A wide range of variability occurs for the subtidal transport variation from subinertial (2–10 days) to interannual scales. While the subinertial variability is shown to arise from the atmospheric pressure disturbances, the longer period variation has been poorly understood.Mean upper circulation of the Ulleung Basin is characterized by the northward flowing East Korean Warm Current along the east coast of Korea and its meander eastward after the separation from the coast, the Offshore Branch along the coast of Japan, and the anticyclonic Ulleung Warm Eddy that forms from a meander of the East Korean Warm Current. Continuous acoustic travel-time measurements between June 1999 and June 2001 suggest five quasi-stable upper circulation patterns that persist for about 3–5 months with transitions between successive patterns occurring in a few months or days. Disappearance of the East Korean Warm Current is triggered by merging the Dok Cold Eddy, originating from the pinching-off of the meander trough, with the coastal cold water carried Southward by the North Korean Cold Current. The Ulleung Warm Eddy persisted for about 20 months in the middle of the Ulleung Basin with changes in its position and spatial scale associated with strengthening and weakening of the transport through the Korea/Tsushima Strait. The variability of upper circulation is partly related to the transport variation through the Korea/Tsushima Strait. Movements of the coastal cold water and the instability of the polar front also appear to be important factors affecting the variability.Deep circulation in the Ulleung Basin is primarily cyclonic and commonly consists of one or more cyclonic cells, and an anticyclonic cell centered near Ulleung Island. The cyclonic circulation is conjectured to be driven by a net inflow through the Ulleung Interplain Gap, which serves as a conduit for the exchange of deep waters between the Japan Basin in the northern East Sea and the Ulleung Basin. Deep currents are characterized by a short correlation scale and the predominance of mesoscale variability with periods of 20–40 days. Seasonality of deep currents is indistinct, and the coupling of upper and deep circulation has not been clarified yet.  相似文献   

青藏高原中部湖泊沉积物中Zr/Rb值及其环境意义   总被引：14，自引：1，他引：14  

陈诗越  王苏民  金章东  沈吉 《海洋地质与第四纪地质》2003,23(4):35-38

由于青藏高原及其周边地区构造复杂,湖泊沉积物中的粒级差别很大,单一的测量方法往往难以奏效。Rb、Zr是表生地球化学过程中的稳定元素,但它们之间也存在显著差别,Rb一般富集在细颗粒中,Zr则在粗颗粒中含量较高。研究发现,湖泊沉积物中Zr/Rb比值与粘土(<2μm)含量存在显著相关,Zr/Rb值反映了湖泊沉积物的粒度大小。Zr/Rb比值所揭示的青藏高原中部280万年来经历的3次大的环境演化过程与岩性变化、孢粉指标反映的环境特征是一致的。  相似文献   

中国北方沿海食用贝类油污染特征研究     

尚龙生  孙茜  王静芳  徐恒振 《海洋科学进展》1996,(4)

１９９０年７月～１９９１年１２月，监测了中国北部沿海食用贝类体内的污染物残留量水平。本文用石油烃的分析数据为基础，讨论了沿岸贝类受石油烃污染的现状和分布特征；计算了样品干、湿重含量的本底值范围。给出了油标准和标准之间的互校系数Ｒ值。对样品进行了同步荧光光谱分析，并用同步荧光光谱的分析数据，计算了样品间模糊相关系数，做了模糊聚类分析。  相似文献   

气相色谱指纹法在海上油污染源鉴别中的应用   总被引：4，自引：0，他引：4  

陈伟琪  张珞平 《海洋科学》2003,27(7):67-70

通过一个具体案例,探讨了气相色谱指纹法在海上油污染源鉴别中的应用。该鉴别方法基于样品的正构烷烃气相色谱指纹分析,选择既能表征油种固有特性,又受风化影响较小的正十七烷(n-C17)、正十八烷(n-C18)、姥鲛烷(phy)、植烷(phy)和正二十烷(n-C20)等特征烷烃作为判据,可信度大,而且整个操作流程短(一般不超过半天),较简便易行。  相似文献   

Detection of free gas and gas hydrate based on 3D seismic data and cone penetration testing: An example from the Nigerian Continental Slope     

N. Sultan  M. Voisset  T. Marsset  A.M. Vernant  E. Cauquil  J.L. Colliat  V. Curinier 《Marine Geology》2007,240(1-4):235-255

We present a new method to characterize free gas, gas hydrates and carbonate concretions occurrence which are considered as high-risk factors for sub-sea developments in the Niger delta. This method is based on the combination of 3D seismic data to the geotechnical site characterizations using piezocone CPTU tests (Cone Penetration Test with additional measurement of the pore water pressure). A special processing of the 3D seismic data has enabled the determination of the interval compressional velocity. Using the effective-medium theory, velocity anomalies (negative and positive) within the first 15 m were translated in gas hydrate and free gas distribution. The calibration of the P wave velocity anomalies was done thanks to in-situ geotechnical testing carried out during two oceanographic surveys (2003 and 2004). Comparison between in-situ testing, recovered cores and the prediction of the gas and the gas hydrate distribution based on the compressional wave velocity have shown that 3D seismic data is a valuable tool to identify heterogeneous areas but the use of the piezocone was essential to discriminate between gas hydrate occurrences and carbonate concretions' presence. Furthermore, in-situ compressional wave velocity (V_p) measurements have clearly demonstrated what it was suspected from the 3D seismic data, the co-existence in the study area between gas hydrate and free gas.  相似文献   

深水资料浮标中温度传感器电路的设计     

唐原广 《海洋科学》1991,15(2):68-71

本文对深水资料浮标中温度传感器的电路原理作了介绍。传感器中的感温元件,采用两端单片集成温度传感器AD590;整个测温电路仅用一片高精度4×1/2位A/D转换器7135;为了能方便地检验和标定温度值,在电路上还设计了温度显示部分,并设计了闭显的功能,以达到省电的目的。  相似文献   

太平洋中部表层沉积物镁铝含量比的变化   总被引：4，自引：0，他引：4  

张士三 《台湾海峡》1990,9(3):244-250

本文根据中、日调查资料,研究了太平洋中部表层沉积物镁铝含量比的变化,并探讨其影响因素。  相似文献   

渐新世/中新世分界的地层学事件   总被引：5，自引：1，他引：5  

李前裕  郑范  刘传联 《海洋地质与第四纪地质》2007,27(5):57-64

介绍渐新世/中新世分界面(即古近纪/新近纪分界面)附近的浮游有孔虫和钙质超微化石事件及其新年龄。2004年国际地层年代表确定该分界面位于第58长偏心率周期弱振幅处,古地磁事件C6Cn.2n底面,经天文调谐后的年龄为23.03 Ma。发生在该界面附近的生物地层学事件主要是浮游有孔虫Paragloborotalia kugleri的始现面(22.96 Ma)和钙质超微化石Sphenolithus delphix的末现面(23.11 Ma)。在南海北部东沙群岛附近的大洋钻探ODP1148站,渐新世/中新世界面以崩塌沉积物为特征,有明显沉积间断,标志南海当时有较大范围的构造运动。  相似文献   

Food habits of harp seals (Phoca groenlandica) during lactation and moult in March-May in the southern Barents Sea and White Sea   总被引：1，自引：0，他引：1  

Nilssen  Kjell T.; Haug  Tore; Potelov  Vladimir; Stasenkov  Vladimir A.; Timoshenko  Yuri K. 《ICES Journal of Marine Science》1995,52(1):33-41

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