Development of a real-time PCR method for Thalassiosira rotula rapid detection     

HE Shanying  YU Zhigang  MI Tiezhu 《海洋学报(英文版)》2007,26(2):133-139

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.  相似文献   

Modeling of the eddy viscosity by breaking waves     

ZHANG Shuwen  YUAN Yeli  ZHENG Quan''an 《海洋学报(英文版)》2007,26(6):116-123

Breaking wave induced nearsurface turbulence has important consequences for many physical and biochemical processes including water column and nutrients mixing,heat and gases exchange across air-sea interface.The energy loss from wave breaking and the bubble plume penetration depth are estimated.As a consequence,the vertical distribution of the turbulent kinetic energy(TKE),the TKE dissipation rate and the eddy viscosity induced by wave breaking are also provided.It is indicated that model results are found to be consistent with the observational evidence that most TKE generated by wave breaking is lost within a depth of a few meters near the sea surface.High turbulence level with intensities of eddy viscosity induced by breaking is nearly four orders larger than υwl(=κuwz),the value predicted for the wall layer scaling close to the surface,where uw is the friction velocity in water,κ with 0.4 is the von Kármán constant,and z is the water depth,and the strength of the eddy viscosity depends both on wind speed and sea state,and decays rapidly through the depth.This leads to the conclusion that the breaking wave induced vertical mixing is mainly limited to the near surface layer,well above the classical values expected from the similarity theory.Deeper down,however,the effects of wave breaking on the vertical mixing become less important.  相似文献   

深水油气田井场调查技术研究     

温明明徐行  杨胜雄 《海洋地质》2005,(2):1-9

随着近海油气不断开发，其后续发展能力明显不足，因此深水含油气盆地的开发将成为必然的发展趋势。深水油气田的井场调查是深水油气田开发过程中的一个主要环节，其勘探技术越来越被人们所关注。本文通过深水井场调查的技术要求分析，结合多次组织和参加近海井场调查的工作经验，指出深水油气田井场调查的技术难点在于用于探索海底表面障碍物分布情况、勘测海底地形地貌特征、了解中浅地层结构等这类勘探技术上。由于这些勘探技术受声纳技术特点的限制，国外已经采用了DEEFTOW、ROV或者AUV技术，将一些关键调查设备与海底保持一定的高度来实现勘探技术目标，并已经取得成功的尝试，因此，研究、发展和不断地完善这类贴近海底多参量勘查技术十分重要。  相似文献   

Ecopath with Ecosim在水生生态系统研究中的应用   总被引：5，自引：0，他引：5  

宋兵  陈立侨  Chen Yong 《海洋科学》2007,31(1):83-86

地球上的水体为人类提供着无法计量的生态系统服务和相应的生态价值。然而,水生生态系统一直承受着巨大的捕捞和环境压力。为更好地分析人类以及自然扰动对水生生态系统的影响,并建立科学合理的管理机制,许多研究种群结构和动态的模型软件以  相似文献   

载人潜水器圆柱形耐压壳体的优化设计研究     

李学斌 《海洋技术学报》2007,26(3):5-8,12

采用分枝定界法和序列二次规划方法,对载人潜水器圆柱形耐压壳体的重量最小化进行了研究.设计变量是壳板的厚度、肋骨的型号、间距和数量,讨论了下潜深度、材料几何参数对重量以及其它特征量的影响.算例计算表明,下潜深度越大,屈服极限越高的材料重量减轻越明显.对于大深度而言,选用高屈服极限材料,可以使得材料能够充分利用.文中还对结构重量占排水量比例随深度变化的情况进行了研究.  相似文献   

河口海岸三维水流数值模型中几种垂向坐标模式研究述评     

陶建峰  张长宽 《海洋工程》2007,25(1):133-142

回顾了近年来国内外河口、近岸海域三维斜压水流数值模式研究的进展,着重讨论了不同垂向坐标下三维水流模式的发展状况以及存在的问题。地形拟合坐标(σ坐标)模式可以很好地拟合床面地形和自由面,已在物理海洋学中被广泛使用。但在地形变化剧烈,尤其在密度层结效应明显的海域,σ坐标模式中的水平压强梯度力误差会引起伪密度流。寻求高精度的数值模式以及更加有效的拟合坐标变换将是值得努力的方向。  相似文献   

A General Linear Wave Theory for Water Waves Propagating over Uneven Porous Bottoms   总被引：1，自引：0，他引：1  

锁要红  黄虎 《中国海洋工程》2004,18(1):163-171

Starting from the widespread phenomena of porous bottoms in the near shore region, considering fully the diversity of bottom topography and wave number variation, and including the effect of evanescent modes, a general linear wave theory for water waves propagating over uneven porous bottoms in the near shore region is established by use of Green‘s scond identity. This theory can be reduced to a number of the most typical mild-slope equations curreutly in use and provide a reliable research basis for follow-up development of nonlinear water wave theory involving porous bottoms.  相似文献   

Mach Effect of Oblique Nonlinear Irregular Waves Interacting with Vertical Walls     

冯卫兵潘丽红  洪广文 《中国海洋工程》2004,18(2):299-306

Based on Hong‘s theory, previous random models, and a generalized expression suitable for FIT calculation, the interaction between irregular waves and vertical walls is numerically simulated. The results of simulation demonstrate that the wave energy changes with the incidence angle and the distance from the wall. Particularly, the Mach effect and the combined wave spectrum characteristics are analyzed in detail, which are significant in both theory and practice.  相似文献   

Probabilistic Models for the Probability of Wave Breaking and Whitecap Coverage Based on Kinematic Breaking Criterion     

郑桂珍  徐德伦 《中国海洋工程》2004,18(3):357-370

More and more researches show that neither the critical downward acceleration nor the critical slope of water waves is a universal constant. On the contrary, they vary with particular wave conditions. This fact moders the models either for the probability of wave breaking B or for the whitecap coverage W based on these criteria difficult to apply. In this paper and the one which follows we seek to develop models for the prediction of both B and W based on the kinematical criterion. First, several joint probabihstic distribution functions (PDFs) of wave characteristics are derived, based on which the breaking properties B and W are estimated. The estimation is made on the assumption that a wave breaks ff the horizontal velocity of water particles at its crest exceeds the local wave celerity, and whitecapping occurs in regions of fluid where water particles travel faster than the waves. The consequent B and W depend on wave spectral moments of orders 0 to 4.Then the JONSWAP spectrum is used to represent the fetch-limited sea waves in deep water, so as to relate the probahility of wave breaking and the whitecap coverage with wind parameters. To this end, the time-averaging technique proposed by Glazman (1986) is applied to the estimation of the spectral moments involved, and furthermore, the theoretical models are compared with available observations collected from published literature. From the comparison, the averaging time scale is determined. The final models show that the probability of wave breaking as well as the whitecap coverage depends on the dimensionless fetch. The agreement between these models and the database is reasonable.  相似文献   

基于塑性极限分析理论和神经网络响应面的结构系统可靠性分析     

闫宏生  胡云昌 《海洋工程》2003,21(2):1-7

根据多层神经网络映射存在定理，提出了基于神经网络响应面和塑性极限分析理论的结构系统可靠性分析的新方法。数值试验表明，该方法可以快速、高质量地求出结构系统可靠性指标，是一个具有发展前景的新方法。  相似文献