两种海洋微藻硝酸还原酶活性测定方法的比较研究   总被引：1，自引：0，他引：1  

唐洪杰  王修林  祝陈坚  李雁宾  张传松 《中国海洋大学学报(自然科学版)》2006,36(6):981-986

对6种常见海洋微藻的硝酸还原酶活性测定方法进行了初步研究。确立了离体法和活体法的提取(振荡)时间及酶促反应时间,分别为:5 min,30 min(离体法)和6 min,10 min(活体法),并对2种方法进行了对比。结果表明:在本文条件下,离体法较活体法更适于进行塔玛亚历山大藻(Alexandrium tamarense)、强壮前沟藻(Amphidinium carterae)、中肋骨条藻(Skeletonema costatum)、新月菱形藻(Nitzchia closterium)和旋链角毛藻(Chaetoceros curvisetus)的硝酸还原酶活性的测定;活体法更适于东海原甲藻(Prorocentrum donghainase)硝酸还原酶活性的测定。  相似文献   

莫桑比克罗非鱼孵卵期间口腔粘膜变化的研究     

楼宝城  林华英  蒋明星 《中国海洋大学学报(自然科学版)》1990,(2)

用组织学、组织化学和酶技术,对雌性莫桑比克罗非鱼在孵卵前后口腔粘膜的组织结构、抗菌酶的比活力变化进行初步研究。结果表明:在口腔粘膜的上皮中,杯状细胞是一个重要的结构,它随着雌鱼的生理状态的改变而变化。雄鱼、雌鱼和孵卵期的雌鱼口腔粘膜上皮中的杯状细胞数量上的差别显著。孵卵期的雌鱼口腔粘膜类似于哺乳动物的子宫粘膜也是受性激素影响的靶器官。该种鱼的口腔粘膜中存在着两种抗菌酶;蛋白质水解酶和壳多糖酶。孵卵前后,这两种酶的比活力分别提高1.4—2.03倍。说明孵卵时,口腔粘膜为使卵及仔鱼健康的发育,增强了抗菌能力。研究结果证明:抗菌力的增加与口腔粘膜中的杯状细胞的大量增生有着密切的关系。  相似文献   

Alkaline Phosphatase Activity in the Red-Tide Dinoflagellate, Ptychodiscus brevis     

G.A. Vargo  E. Shanley 《Marine Ecology》1985,6(3):251-264

Abstract. Alkaline phosphatase activity (APA) was found in a nearshore, natural population of the red-tide dinoflagellate Ptychodiscus brevis. Initial hydrolysis rates were enhanced by two orders of magnitude within 24 h upon addition of ammonium. Other nutrient (additions) or no additions also lead to a higher, although less pronounced increase in activity. Enhanced growth after nutrient additions combined with specific APA indicated that the bloom was both N and P limited. Since the natural population was a mixture of diatoms and the dinoflagellate, APA was also measured in unialgal cultures of P. brevis. APA was not reduced but enhanced by the addition of low concentrations of orthophosphate (0.5 and 1 μM). Higher orthophosphate concentrations (5 to 20 μM) reduced APA, although activity returned to control levels within 2 to 6h. Repression occurred within 30 sec after a 20 μM orthophosphate addition. Cells which had APA repressed by an orthophosphate addition regained their initial activity within 2 h after washing with phosphate-free media. Phosphorus-deficient cells of P. brevis possess an alkaline phosphatase system which is capable of continued activity at low in situ orthophosphate levels.  相似文献   

Target-strength measurements of Pacific hake (Merluccius productus) in Canadian waters using quasi-ideal and conventional beam transducers     

Hamano  Akira; Sasakura  Toyoki; Kieser  Robert; Cooke  Ken; Kubota  Katsuhiko; Clay  Allen 《ICES Journal of Marine Science》1996,53(2):273-279

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Shallow-Water Gasohydrothermal Vents of Ushishir Volcano and the Ecosystem of Kraternaya Bight (The Kurile Islands)   总被引：2，自引：0，他引：2  

V. G. Tarasov    M. V. Propp    L. N. Propp    A. V. Zhirmunsky    B. B. Namsakakv    V. M. Gorlenko  D. A. Starynin 《Marine Ecology》1990,11(1):1-23

Abstract. A marine ecosystem in the crater of the Ushishir Volcano (Kraternaya Bight, Yankich Island, the Kuriles) showing gasothcrmal activity was studied for hydrographical, hydrochemical, and biological characteristics. Maximal changes in chemical and biological characteristics were observed in the surface water layer of 0–5 m. This layer had higher water temperature, was saturated with volcanogcnic carbon dioxide (up to 2000 10^-6 torr), ovcrsaturatcd with oxygen (up to 200 %), and contained high concentrations of chlorophyll a. Hydrogen sulfide was found in the surface water layer and at a depth of 15 m in the area of maximal effect of volcanic effluents.
The planktonic community is characterized by high rates of production and destruction of organic matter only in the 0–5 m layer. Daily vertical migrations of the ciliatc Mesodinium rubrum were observed, which caused "red tides".
Bacterial, algobacterial, and diatom mats developed on the bottom of the bight in the zone of gasohydrothermal vents and in areas of volcanic water seeping. The rate of organic matter production in algobacterial mats reached 33.4g C-rrr²-d^-l, chcmolithotrophy predominating. Bottom settlements had high population density and great animal biomass, which reached 10 kg m^-2 in gasohydrothermal fields. They obtain sufficient energy from primary production of microphy-tobenthos, algobacterial, and bacterial mats. Bcnthic species dominant in the bight were not found off the Ushishir Islands.
Some species of macrobenthos inhabiting the Kraternaya Bight differ markedly in size and biomass from the same species found beyond the bight.  相似文献   

Nature of the connection between the Northern and Southern Zechstein Basins across the Mid North Sea High     

M.K. Jenyon  P.M. Cresswell  J.C.M. Taylor 《Marine and Petroleum Geology》1984,1(4):355-363

The seismic expression of a salt-filled channel which cuts across the Mid North Sea High in Quadrant 37 is described, with features interpreted as being produced by salt-edge dissolution forming both eastern and western margins of the channel. The apparent half-graben nature of the channel is shown to be only superficial, and due to complex faulting associated with, but not defining, its western margin. The shallower faulting here is a Mesozoic to early Tertiary growth fault related to local dissolution of Zechstein salt. The dissolution effect appears in turn to have been localized by the presence of a deeper fault that was already downthrown to the east in Zechstein times, when it seems to have limited the eastward progradation of Zechstein shelf carbonates and anhydrites, and had probably ceased to move significantly before the onset of the Late Cimmerian erosional phase. The origin of this arcuate fault is tentatively ascribed to subsidence around a granite batholith. Zechstein salt originally spread some distance to the east and west of the channel; it was dissolved from the edges inwards, mainly before the Late Cretaceous, giving rise to a thicker Mesozoic sequence on parts of the flanks of the channel than in the middle. Besides providing an interesting structural case history, the features described have implications regarding Zechstein sedimentation, reservoir potential, the tectonic history of the North Sea, and the nature of the Mid North Sea High itself.  相似文献   

甘糖酯对大鼠尿激酶型纤溶酶原激活物活性的诱导作用     

姚子昂  马洪明  姜招峰  管华诗 《中国海洋大学学报(自然科学版)》2001,31(2):215-219

为了研究甘糖酯对大鼠体内尿激酶型纤溶酶原激活物 (urokinase PlasminogenActivator,u PA)活性的影响 ,采用溶解圈中和抑制法和发色底物法检测了大鼠口服甘糖酯后体内u PA活性的变化情况。结果表明 :甘糖酯可提高大鼠体内血液的纤溶活性 ,主要表现为 u PA活性升高。以不同剂量的甘糖酯连续喂药 10 d后 ,发现喂药组大鼠 u PA活性明显高于对照组 ,喂药组大鼠血浆 u PA活性升高同喂药剂量正相关 ,在 0～ 10 0 mg/ kg的剂量范围内 ,随喂药剂量增加而升高。提示在一定的剂量范围内甘糖酯可提高大鼠体内 u PA活性 ,激活纤溶系统 ,提高血液纤溶活性 ,表现出良好的抗栓作用  相似文献   

Stability of waterfront retaining wall subjected to pseudo-static earthquake forces     

Deepankar Choudhury  Syed Mohd. Ahmad 《Ocean Engineering》2007,34(14-15):1947-1954

Waterfront retaining walls supporting dry backfill are subjected to hydrostatic pressure on upstream face and earth pressure on the downstream face. Under seismic conditions, if such a wall retains a submerged backfill, additional hydrodynamic pressures are generated. This paper pertains to a study in which the effect of earthquakes along with the hydrodynamic pressure including inertial forces on such a retaining wall is observed. The hydrodynamic pressure is calculated using Westergaard's approach, while the earth pressure is calculated using Mononobe-Okabe's pseudo-static analysis. It is observed that when the horizontal seismic acceleration coefficient is increased from 0 to 0.2, there is a 57% decrease in the factor of safety of the retaining wall in sliding mode. For investigating the effect of different parameters, a parametric study is also done. It is observed that if φ is increased from 30° to 35°, there is an increase in the factor of safety in the sliding mode by 20.4%. Similar observations were made for other parameters as well. Comparison of results obtained from the present approach with [Ebeling, R.M., Morrison Jr, E.E., 1992. The seismic design of waterfront retaining structures. US Army Technical Report ITL-92-11. Washington DC] reveal that the factor of safety for static condition (k_h=0), calculated by both the approaches, is 1.60 while for an earthquake with k_h=0.2, they differ by 22.5% due to the consideration of wall inertia in the present study.  相似文献   

Elemental geochemical records of seafloor hydrothermal activities in the sediments from the Okinawa Trough     

ZHAI Shikui  YU Zenghui  DU Tongjun 《海洋学报(英文版)》2007,26(4):53-62

The major and minor element contents in the sediment core H9 from the hydrothermal fields of the Okinawa Trough show a sharp change at the depth of 80 cm. The elements enriched in the upper 80 cm core are those enriched in the hydrothermal deposits and in the surface sediments recovered from the hydrothermal fields in the trough, which indicates the input of hydrothermal materials. Comparing with other hydrothermal sediments from Mid-ocean Ridges or the Lau Basin, the degree of the enrichment of elements iron, copper,cobalt, and nickel is relatively low. However, the enrichment of elements manganese, lead, arsenic, antimony and mercury is remarkable. The average contents of these elements in the upper 80 cm core sediments are three to six times those in the lower section, and 3~12 times those in the surface sediments which are not influenced by hydrothermal activities. Hydrothermal activities have contributed significant manganese, lead, arsenic, antimony and mercury to the sediments, and these elements are distinct indicators for the hydrothermal activity in the Okinawa Trough. The significant enrichment of these elements in Core H9 upward from the depth 80 cm indicates the start or the significant enhancing of the hydrothermal activity in this area at about 5 740 aB.P. The average accumulation rate of manganese during this period is about 40 461 μg/(cm2·ka), which is similar to the hydrothermal sediments in the Lau Basin or the East Pacific Rise.  相似文献   

山东沿海海藻抗肿瘤活性的筛选   总被引：28，自引：6，他引：28  

徐年军  范晓  韩丽君  严小军  曾呈奎 《海洋与湖沼》2001,32(4):408-413

于1997年4月和2000年5月对山东沿海海藻进行较系统的采集，对其中39种海藻的96个样品采用高通量方法进行选择性细胞毒筛选，结果表明，小粘膜藻（Leathesia dif-formes)、多管藻（Polysiphonia urcedata)、萱cytosiphon lomentarius)、海萝（Gloiopeliis fur-cata)、叉开网翼藻（Dictyopteris diuaricata)、点叶菜（Punctaria latifolia)等对KB细胞或HT-29细胞具有选择性抑制活性。局醇、氯仿的提取效率较高，多管藻的乙醇、氯仿提取物，萱藻的乙醇提取物和叉开网翼藻的正己烷提取物都有强的选择性细胞毒活性。  相似文献