Zooplankton community analysis in the Changjiang River estuary by single-gene-targeted metagenomics     

程方平  王敏晓  李超伦  孙松 《中国海洋湖沼学报》2014,32(4):858-870

DNA barcoding provides accurate identification of zooplankton species through all life stages. Single-gene-targeted metagenomic analysis based on DNA barcode databases can facilitate longterm monitoring of zooplankton communities. With the help of the available zooplankton databases, the zooplankton community of the Changjiang (Yangtze) River estuary was studied using a single-gene-targeted metagenomic method to estimate the species richness of this community. A total of 856 mitochondrial cytochrome oxidase subunit 1 (cox1) gene sequences were determined. The environmental barcodes were clustered into 70 molecular operational taxonomic units (MOTUs). Forty-two MOTUs matched barcoded marine organisms with more than 90% similarity and were assigned to either the species (similarity>96%) or genus level (similarity<96%). Sibling species could also be distinguished. Many species that were overlooked by morphological methods were identified by molecular methods, especially gelatinous zooplankton and merozooplankton that were likely sampled at different life history phases. Zooplankton community structures differed significantly among all of the samples. The MOTU spatial distributions were influenced by the ecological habits of the corresponding species. In conclusion, single-gene-targeted metagenomic analysis is a useful tool for zooplankton studies, with which specimens from all life history stages can be identified quickly and effectively with a comprehensive database.  相似文献   

Production and Certification of a Unique Set of Isotope and Delta Reference Materials for Boron Isotope Determination in Geochemical,Environmental and Industrial Materials     

Jochen Vogl  Martin Rosner 《Geostandards and Geoanalytical Research》2012,36(2):161-175

Isotopic reference materials are essential to enable reliable and comparable isotope data. In the case of boron only a very limited number of such materials is available, thus preventing adequate quality control of measurement results and validation of analytical procedures. To address this situation a unique set of two boron isotope reference materials (ERM‐AE102a and ‐AE104a) and three offset δ¹¹B reference materials (ERM‐AE120, ‐AE121 and ‐AE122) were produced and certified. The present article describes the production and certification procedure in detail. The isotopic composition of all the materials was adjusted by mixing boron parent solutions enriched in ¹⁰B or ¹¹B with a boron parent solution having a natural isotopic composition under full gravimetric control. All parent solutions were analysed for their boron concentration as well as their boron isotopic composition by thermal ionisation mass spectrometry (TIMS) using isotope dilution as the calibration technique. For all five reference materials the isotopic composition obtained on the basis of the gravimetric data agreed very well with the isotopic composition obtained from different TIMS techniques. Stability and homogeneity studies that were performed showed no significant influence on the isotopic composition or on the related uncertainties. The three reference materials ERM‐AE120, ERM‐AE121 and ERM‐AE122 are the first reference materials with natural δ¹¹B values not equal to 0‰. The certified δ¹¹B values are ?20.2‰ for ERM‐AE120, 19.9‰ for ERM‐AE121 and 39.7‰ for ERM‐AE122, each with an expanded uncertainty (k = 2) of 0.6‰. These materials were produced to cover about three‐quarters of the known natural boron isotope variation. The ¹⁰B enriched isotope reference materials ERM‐AE102a and ERM‐AE104a were produced for industrial applications utilising ¹⁰B for neutron shielding purposes. The certified ¹⁰B isotope abundances are 0.29995 for ERM‐AE102a and 0.31488 for ERM‐AE104a with expanded uncertainties (k = 2) of 0.00027 and 0.00028, respectively. Together with the formerly certified ERM‐AE101 and ERM‐AE103 a unique set of four isotope reference materials and three offset δ¹¹B reference materials for boron isotope determination are now available from European Reference Materials.  相似文献   

Research Status and Advance in Carbon Isotope (δ13C) Variation from Stalagmite     

Huang Wei  Liu Dianbing  Wang Luyao  Zhang Zhenqiu 《地球科学进展》2016,31(9):968-983

The application of carbon isotope (δ¹³C) collected from stalagmite to reconstruct the past climate and/or ecologic evolution, relative to other preserved indicators, is much limited due to its complex influencing factors including climate outside cave and complicated and site-specific karstic process. In terms of various climate and non-climate limiting factors on stalagmite δ¹³C, and combined with a large number of geological records and modern cave monitoring data of the latest researches, the pathway and behavior of the signal transmission of δ¹³C in the cave system were analyzed. The possible relationship between δ¹³C excursions and climate oscillations under different time scales was thus discussed. Although the degree of noise becomes increasing mixed with δ¹³C on centennial scales or shorter, the climatic elements, such as temperature and humidity (or precipitation), as predominant modulators exert directly or indirectly influence on vegetation overlying the soil and associated soil CO₂ productivity on millennial-orbital scales. Future work should be focused on further deeply extracting the common δ¹³C signals from specific caves by exploring the controlling factors, both including climatic and non-climatic ones, attaching importance to the seasonal characteristics of stalagmite δ¹³C, and taking full advantage of its potential in the indication of local environmental events. The use of multi-proxy and multi-method will contribute to better understanding the interesting linkages among the δ¹³C characteristics, karstic process, global carbon cycle and associated climate change.  相似文献   

Elemental abundance analyses with DAO spectrograms: XXXIII. β UMa (A0mA1 IV‐V), α Dra (A0 III), π Dra (A2 IIIs), and κ Cep (B9 III)     

S.J. Adelman  K. Yu  A.F. Gulliver 《Astronomische Nachrichten》2011,332(2):153-158

This paper presents extended analyses of β UMa (A0mA1 IV‐V), α Dra (A0 III), π Dra (A2 IIIs), and κ Cep (B9 III) which have previously been studied in this series. α Dra is a metal‐poor star while κ Cep has solar abundances. Both β UMa and π Dra are Am stars. Whenever possible, more accurate and precise gf values replace older values. High S/N (200+) and high dispersion Dominion Astrophysical Observatory spectrograms to the red of previously obtained spectra supplement the observations. The derived rotational velocities are 45, 25, 26, and 23 km s^–1, respectively. These LTE fine analyses use the ATLAS9 and the WIDTH9 programs of R. L. Kurucz. The results of the extended and the previous analyses are in good agreement. Thus in the past decade a significant improvement in the system of gf values has not been achieved although for many lines there have been changes. The use of additional regions has increased the quality of some results (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)  相似文献   

First detection of a magnetic field in the fast rotating runaway Oe star ζ Ophiuchi     

S. Hubrig  L.M. Oskinova  M. Schller 《Astronomische Nachrichten》2011,332(2):147-152

The star ζ Ophiuchi is one of the brightest massive stars in the northern hemisphere and was intensively studied in various wavelength domains. The currently available observational material suggests that certain observed phenomena are related to the presence of a magnetic field. We acquired spectropolarimetric observations of ζ Oph with FORS 1 mounted on the 8‐m Kueyen telescope of the VLT to investigate if a magnetic field is indeed present in this star. Using all available absorption lines, we detect a mean longitudinal magnetic field 〈B_z〉_all = 141 ± 45 G, confirming the magnetic nature of this star. We review the X‐ray properties of ζ Oph with the aim to understand whether the X‐ray emission of ζ Oph is dominated by magnetic or by wind instability processes (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)  相似文献   

Looking for a massive KeV pseudo-scalar in gamma ray bursts     

C. Wolf 《Astronomische Nachrichten》1992,313(3):133-137

Looking for repetitive signals within a γ burst may provide evidence for a massive KeV pseudo-scalar coupled to electromagnetism. We study the possible signatures that may occur for pseudo-scalar electromagnetic propagation and outline possible probes to the pseudo-scalar mass as well as its coupling to electromagnetism.  相似文献   

Rapid oscillations of five stars in the spectral range A and F. I     

G. Hildebrandt 《Astronomische Nachrichten》1992,313(4):233-241

The five stars HD 4849, HD 24712, HD 182475, HD 184552 and HD 215874 are a small group of objects enclosed in a larger observational programme for searching of rapid oscillations. They have been observed with the 50 cm ESO-telescope at La Silla (Chile). These objects are characterized as a suspected δ Scuti-type star, an Ap star, an A9 V star, an A1m star and an A9 III-IV star, respectively. For the first two stars the periods, dedected by other authors, were confirmed. For HD 182475 a period of 112.3 min has been estimated. In the case of HD 184552 an oscillation with a period of 163.3 min has been found. The star HD 215874 shows unambiguously a variation with a period of 97.2 min.  相似文献   

Exposure to extreme climate decreases self-rated health score: Large-scale survey evidence from China     

《Global Environmental Change》2022

High temperature is a growing threat and impacts public health through different exposure mechanisms. Our study constructs a comprehensive exposure measurement based on temperature variability, duration, and effective influence range. We investigate human responses to high temperatures through self-rated health scores based on individual-level data from China Labor-force Dynamic Survey (CLDS). Results show that higher temperature and temperature variability significantly decrease self-rated health scores. Subjective health risk is most significantly related to the cumulative temperature in the previous two weeks. We also find that the exposure effects at night and on weekdays are more severe. Workers who experience greater exposure from commuting and work environments are negatively impacted by high temperatures. In addition, men, the elderly, middle and low education groups, rural residents are more likely to be impacted by high temperatures.  相似文献