Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri     

YU Ziniu  WEI Xiaohu  KONG Xiaoyu  YU Shanshan 《海洋学报(英文版)》2007,26(1):93-100

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.  相似文献   

A Three-Point Method for Separating Incident and Reflected Waves over A Sloping Bed     

CHANG Hsien-Kuo 《中国海洋工程》2002,16(4):499-511

This study presents a three-point method for separating incident and reflected waves to explain normally incident waves' propagating over a sloping bed. Linear wave shoaling is used to determine changes in wave amplitude and phase in response to variations of bathymetry. The wave reflection coefficient and incident amplitude are estimated from wave heights measured at three fixed wave gauges with unequal spacing. Sensitivity analysis demonstrates that the proposed method can predict the reflection and amplitude of waves over a sloping bed more accurately than the two-point method.  相似文献   

福州市马尾地区第四纪地层划分及海相层分析   总被引：1，自引：0，他引：1  

韩书华  张静 《海洋地质与第四纪地质》1992,12(1):85-96

通过对该区钻孔剖面贡料,结合~(14)C、古地磁、孢粉及微体古生物等资料综合分析研究,对该区第四纪地层提出划分意见和自第四纪晚期以来,地层中存在着典型两期海侵所形成的海相层。  相似文献   

Does subterranean flow initiate mud banks off the southwest coast of India?     

K. K. Balachandran 《Estuarine, Coastal and Shelf Science》2004,59(4):589-598

Coastal waters off the southwest coast of India draw special attention because of the occurrence of mud banks at certain locations during southwest monsoon period. The present study puts forward a hypothesis of a subterranean flow, which could be a plausible mechanism to initiate the mud banks. The subterranean flow is believed to be coupled with activated trending faults and originate from the adjacent watershed (Vembanad Lake) separated from the sea by a narrow strip of land where submerged porous lime shell beds are present. When the lake water injection occurs through the fault, the mud/clay gets excited by its thixotrophic properties (as the overlying water looses its electrolyte) and transforms into a flowing fluid. The lowering of salinity due to the introduction of fresh water keeps the mud suspension in the water column for longer duration, leading to the formation of mud banks. The idea of subterranean flow through lime shell beds initiating formation of mud banks may apply globally to any coastal regions hugged by wetlands and of similar geological conditions.  相似文献   

A technique for conducting seismic refraction experiments on the ocean bed using bottom shots     

R. E. Kirk  K. Robertson  R. B. Whitmarsh  P. R. Miles 《Marine Geophysical Researches》1991,13(2):153-160

A technique has been devised for firing arrays of bottom shots on the ocean bed in depths upto 4000 m or more. Ten kilogram explosive charges are dropped from the surface while the shooting ship is navigated acoustically. They are detonated at preset times by an electronic timer which initiates an electrical detonator, detonating cord and cast PETN/TNT explosive. Ranges to ocean bottom seismographs, and the shot instants, can be calculated from the arrival-time differences of the direct and surface-reflected water waves. The accuracy, which is dependent on water-depth and range, was better than 22 m in range and 14 msec in shot instant for our experiments.  相似文献   

Impact of Montmorillonite and Calcite on Release and Adsorption of Cyanobacterial Fatty Acids at Ambient Temperature   总被引：1，自引：0，他引：1  

刘邓  杨小芬  王红梅  李继红  苏念 《中国地质大学学报(英文版)》2008,19(5):526-533

Minerals might act as important sorbents of sedimentary organic matter and reduce biodegradation, which favors the formation of hydrocarbon source rocks in the earth's history. Since most organic matter is degraded during the sinking process, at ambient temperature, it is important to investigate the adsorption capacity of different minerals during this process, to assess the organic loss from primary productivity to sedimentary organic matter. In this study, montmorillonite and calcite have been selected to study the impact of different minerals on the release, adsorption, and deposition of cyanobacterial (Synechococcus elonpata) fatty acids (FAs) at ambient temperature. Gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS) have been utilized to detect the variation in fatty acids. Primary results suggest that minerals have a different impact on dissolved organic matter. Montmorillonite can specifically enhance the release of fatty acids from cyanobacterial cells by lowering the pH values of the solution. The adsorption of the dissolved organic matter by montmorillonite will also be enhanced under a lower pH value. Conjunction of fatty acids with montmorillonite to form a complex will favor the sinking and preservation of these organics. Selective adsorption is observed among fatty acids with different carbon numbers. In contrast, calcite does not show any impact on the release and adsorption of organic matter even though it is reportedly capable of acting as a catalyst during the transformation of organic matter at high temperature. The primary data bridge a link between primary productivity and sedimentary organic matter, suggesting the relative importance of claystones in the formation of hydrocarbon source rocks in the earth's history.  相似文献   

黄河内蒙古段上覆水、悬浮物和底泥重金属特征及生态风险研究   总被引：24，自引：12，他引：12  

赵锁志  刘丽萍  王喜宽  李世宝  朱锁  郑萍  张青  张宝  蒙奎文 《现代地质》2008,22(2):304-312

系统地采集黄河内蒙古段上覆水、悬浮物和底泥样品,分析其中重金属元素的含量。采用地质累积指数法和潜在生态危害系数法,研究了黄河干流内蒙古段的上覆水、悬浮物和底泥的重金属沿程分布特征、污染程度和生态风险。分析结果显示:除了As元素,悬浮物中重金属元素含量总体上高于底泥中的含量。地质累积指数法评价结果表明:悬浮物和底泥属于无污染—中度污染,悬浮物总体上比底泥污染严重;潜在生态危害系数法评价结果表明:底泥和悬浮物中重金属的生态危害总体上属轻微状态;因此,生态风险指数法和地质累积指数评价结果基本一致。  相似文献   

Application of Spectral Decomposition to Detection of Fracture-Cavity Carbonate Reservoir Beds in the Tahe Oiifieid, Tarim Basin, NW China     

Liu Xiaoping  Yang Xiaolan  Zhang Yazhong  Han Long 《《地质学报》英文版》2008,82(3):530-536

Ordovician fracture-cavity carbonate reservoir beds are the major type of producing formations in the Tahe oilfield, Tarim Basin. The seismic responses of these beds clearly changes depending on the different distance of the fracture-cavity reservoir bed from the top of the section. The seismic reflection becomes weak or is absent when the fracture-cavity reservoir beds are less than 20 ms below the top Ordovician. The effect on top Ordovician reflection became weaker with deeper burial of fracture-cavity reservoir beds but the developed deep fracture-cavity reservoir beds caused stronger reflection in the interior of the Ordovician. This interior reflection can be divided into strong long-axis, irregular and bead string reflections, and was present 80 ms below the top Ordovician. Aimed at understanding reflection characteristics, the spectral decomposition technique, which uses frequency to ＂tune-in＂ bed thickness, was used to predict Ordovician fracture-cavity carbonate formations in the Tahe oilfield. Through finely adjusting the processing parameters of spectral decomposition, it was found that the slice at 30 Hz of the tuned data cube can best represent reservoir bed development. Two large N-S-trending strong reflection belts in the mid-western part of the study area along wells TK440- TK427-TK417B and in the eastern part along wells TK404-TK409 were observed distinctly on the 30 Hz slice and 4-D time-frequency data cube carving. A small N-S trending reflection belt in the southern part along wells T403-TK446B was also clearly identified. The predicted reservoir bed development area coincides with the fracture-cavities connection area confirmed by drilling pressure testing results. Deep karst cavities occur basically in three reservoir bed-development belts identified by the Ordovician interior strong reflection. Spectral decomposition proved to be a useful technique in identifying fracture-cavity reservoir beds.  相似文献   

长江流域沿江镉异常源追踪与定量评估的方法技术研究:以长江流域安徽段为例   总被引：5，自引：1，他引：4  

赵传冬  陈富荣  陈兴仁  赵和苍  夏威岚  聂海峰  孔牧  刘飞  杨柯 《地学前缘》2008,15(5):179

长江全流域性的Cd异常是被中国正在进行的多目标地球化学调查发现的重大生态环境问题。以长江流域安徽段为研究对象,对沿江镉异常源追踪与定量评估的方法技术进行了系统研究。通过对安徽段长江干流及其主要支流悬浮物中元素含量的测量查明:悬浮物是流域内重金属元素大跨度迁移的主要载体;Cd在悬浮物中的富集程度远远大于其他重金属元素,这或许正是Cd可以形成沿江流域性异常的主要原因;长江干流悬浮物中重金属元素含量的变化明显受到沿江支流的影响。安徽段长江干流及其主要支流重金属元素输出通量定量计算结果表明:秋蒲河是安徽段重金属元素输出通量最大的支流,每年在安徽段长江两岸土壤中沉积下约4.45t的Cd。利用悬浮物加密测量、1∶5万土壤测量和岩石测量对顺安河流域Cd异常源追踪结果显示:内生金属矿床特别是铅锌矿是悬浮物中Cd的最大的供应源。采用河漫滩沉积物分层采样技术和同位素测年技术,初步恢复了研究区Cd等重金属元素沉积和污染的地球化学历史,对研究区Cd等重金属元素异常的未来演变趋势进行了预警预测。  相似文献   

邵于庄煤炭勘查区新生界松散层水文地质特征     

云晓鸣  高艳  李云峰 《中国煤炭地质》2008,(12):53-54

邵于庄煤炭勘查区新生界松散层厚度较厚,下伏为二叠系含煤地层,可采煤层多。区内含、隔水层组成复杂,由四个含水层和三个隔水层组成。通过对含水层水文地质条件分析,提出了第一、二含水层是矿区的主要供水水源,为保证未来建设和开采时的生活用水和工业用水,应做好水源的保护工作;同时指明了第四含水层是浅部煤组开采的主要补给水源,在煤矿设计和开采过程中,应重点予以防治。  相似文献