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51.
研究肝素酶Ⅰ(来源于Flavobacterumheparinase,EC4.2.2.7)对人工合成的且含有与抗凝血酶Ⅲ特定结合位点的肝素五糖(SHP)的酶解作用,并对酶解作用的动力学进行研究。利用强阴离子高效液相色谱(SAX-HPLC)对酶解混合物进行分离,利用质谱(ESIMS)和核磁共振波谱(1H-NMR)技术对得到的二糖和三糖的结构进行确证。研究结果表明,这种被作为肝素反向试剂的肝素酶Ⅰ可水解人工所合成的肝素五糖,从而使之丧失抗Xa因子活性。 相似文献
52.
海洋木栖真菌抗菌活性的初步研究 总被引:6,自引:0,他引:6
从福建厦门海沧、集美及漳州浮宫等地采集到的红树林等海洋潮间带的各种腐木样品中分离得到176株海洋木栖真菌,对其进行抗菌活性检测.其结果表明共有96株海洋木栖真菌对大肠杆菌、枯草杆菌及白色假丝酵母中的一种或多种拮抗指示菌具有抑制作用,占供测菌株总数的54.5%.在这些活性菌株中,红树源菌株拮抗比例为30.2%,非红树源菌株拮抗比例为69.8%.具有抗菌活性的菌株主要分布于15个属中,包括木霉Trichoderma、青霉Penicillium、拟青霉Paecilomyces和一些不产孢的分类群.某些稀有真菌如盘多毛孢Pestalotia、茎点霉Phoma也具有一定的抗菌活性. 相似文献
53.
54.
Takushi Niki Miwa Shimizu Ayako Fujishiro Junji Kinoshita 《Journal of Oceanography》2007,63(5):873-877
During time-series observations in Sagami Bay, Japan, the concentration of dissolved dimethylsulfoniopropionate (DMSPd), a precursor of dimethylsulfide (DMS), was negatively correlated with salinity. In the laboratory, low-salinity shock reduced
DMS production rates of the natural bacterial community and induced rapid DMSP release from a dinophyte, Heterocapsa triquetra, suggesting that low-salinity shock reduced DMSPd consumption but enhanced DMSPd production, which agrees with the negative correlation between DMSPd and salinity observed in Sagami bay. In addition, low-salinity shock did not affect DMSP lyase activity of H. triquetra. Low-salinity shock would increase the contribution from algae in DMS production, leading to an increase in potential DMS
productivity in the environment. 相似文献
55.
Natalia V. Zhukova 《Ocean Science Journal》2005,40(3):34-42
Variation in the microbial biomass and community structure found in sediment of heavily polluted bays and the adjacent unpolluted
areas were examined using phospholipid fatty acid analysis. Total microbial biomass and microbial community structure were
responding to environmental determinants, sediment grain size, depth of sediment, and pollution due to petroleum hydrocarbons.
The marker fatty acids of microeukaryotes and prokaryotes - aerobic, anaerobic, and sulfate-reducing bacteria -were detected
in sediments of the areas studied. Analysis of the fatty acid profiles revealed wide variations in the community structure
in sediments, depending on the extent of pollution, sediment depth, and sediment grain size. The abundance of specific bacterial
fatty acids points to the dominance of prokaryotic organisms, whose composition differed among the stations. Fatty acid distributions
in sediments suggest the high contribution of aerobic bacteria. Sediments of polluted sites were significantly enriched with
anaerobic bacteria in comparison with clean areas. The contribution of this bacterial group increased with the depth of sediments.
Anaerobic bacteria were predominantly present in muddy sediments, as evidenced from the fatty acid profiles. Relatively high
concentrations of marker fatty acids of sulfate-reducing bacteria were associated with organic pollution in this site. Specific
fatty acids of microeukaryotes were more abundant in surface sediments than in deeper sediment layers. Among the microeukaryotes,
diatoms were an important component. Significant amounts of bacterial biomass, the predominance of bacterial biomarker fatty
acids with abundance of anaerobic and sulfate-reducing bacteria are indicative of a prokaryotic consortium responsive to organic
pollution. 相似文献
56.
Takushi?NikiEmail author Taiki?Fujinaga Mariyo?F.?Watanabe Junji?Kinoshita 《Journal of Oceanography》2004,60(5):913-917
Solid-phase microextraction (SPME) is a simple, sensitive and less destructive method for the determination of dimethylsulfide
(DMS) in seawater. Combined with detection by gas chromatography-mass spectrometry (GC-MS), the method had sufficient sensitivity
(minimum detectable concentration of DMS was 0.05 nM), and practical levels of reproducibility (relative standard deviation
≤7%) and linearity (r
2 > 0.995) over a wide concentration range (0.5 to 910 nM). The protocol developed was applied to a Sagami Bay water sample
to determine concentrations of DMS and DMSP, and in situ DMSP-lyase activity. 相似文献
57.
The Fauna of Floating Cyanobacterial Mats in the Oligohaline Eulittoral Zone off Hiddensee (South-west Coast of the Baltic Sea) 总被引:2,自引:0,他引:2
Abstract. The fauna — including macrofauna, meiofauna, and large ciliates — of floating cyanobacterial mats in a brackish shallow-water area was studied by analysing six 20 cm2 pieces of mat. Although these microbial aggregations were scarcely 1 cm thick, their total meiofauna abundance was about five times as high as in the uppermost 4 cm of the adjacent sediment. The mat fauna was dominated by harpacticoids, although large ciliates, rotifers, nematodes, and oligochaetes were also markedly more abundant than in the sediment. All species occurring in the mats were members of the surrounding sediment fauna. Out of the 47 species found, only a few, among them predominantly the harpacticoid, Mesochra lilljeborgi B oeck , 1864, and the nematode Daptonema setosum ( bütschli , 1874), accounted for the majority of the individual abundance in the mats. Both are regularly found in sulphidic biotopes near Hiddensee. As SEM micrographs revealed, the oligochaete Paranais litoralis (O. F. M üller , 1788) and the harpacticoid Cletocamptus confluens (Schmeil, 1894) were apparently optimal substrates for dense lawns of cyanobacteria. This indicates possible close interactions between the meiofauna and these microbiota. The frequent predation of histophagous ciliates on nematodes and harpacticoids, which were probably weakened by oxygen deficiency and/or high sulphide concentrations, were not only a sign of a generally neglected pathway in the food chain, but also impressively emphasized the huge variety of interactions taking place between meio- and microfauna within this specific benthic microcosm. Because of their floating character, the mats can play an important role in the dispersal of benthic fauna. 相似文献
58.
钝顶螺旋藻藻蓝蛋白和多糖的抗肿瘤免疫活性研究 总被引:14,自引:2,他引:14
对钝顶螺旋藻藻蓝蛋白 (PC)和多糖 (PSP)的抗肿瘤免疫活性进行了研究。采用免疫酶标技术、MTT法及定量溶血分光光度测定法等免疫分析实验 ,从免疫器官、免疫细胞、免疫分子 3个水平上进行藻蓝蛋白和多糖的抗肿瘤免疫活性检测。结果显示 ,藻蓝蛋白和多糖处理组小鼠瘤块直径及瘤体重量均小于对照组 ,T细胞及 B细胞活性明显增强 ,体液抗体量显著提高 ,螺旋藻藻蓝蛋白比多糖抑制肿瘤细胞生长和提高机体免疫力的作用更明显。 相似文献
59.
利用臭氧氧化实现复合生物反应器污泥减量 总被引:3,自引:0,他引:3
采用复合生物反应器,对应用臭氧氧化实现污泥减量进行了研究。2个相同的复合生物反应器平行运行,1个作为对照系统,1个作为氧化系统。反应器内装有半软性填料,投加量为10%。曝气池中悬浮污泥浓度为1 500 mg/L左右,生物膜浓度为2 000 mg/L左右。试验结果表明,随着臭氧氧化污泥比例的增加,污泥表观产率系数也随之降低,当臭氧投量为0.05 g O3/gSS,每天氧化的污泥分别为反应器内污泥的10%,20%,30%时,污泥表观产率系数分别减少28.2%,44.9%,75.8%。虽然随着污泥氧化比例的增大,氧化系统出水CODcr略有增加,但氧化系统仍能保持其生物处理能力,CODcr去除率在92%以上;2个系统之间氨氮的去除率相差不大,氧化系统的硝化能力基本没有受到臭氧氧化的影响。 相似文献
60.
乙醇对文昌鱼酸性磷酸酶活力与构象的影响 总被引:1,自引:0,他引:1
文昌鱼酸性磷酸酶是一种含有铁离子的金属酶,酶的紫外-可见光谱扫描,280nm,500nm有最大吸收峰,320nm有肩峰。荧光扫描:在281nm激发下,330nm有发射峰,在480nm激发下,515nm有一发射峰。 相似文献