变性梯度凝胶电泳在微生物多样性分析中的应用及其技术发展     

徐玲玲  刘亚洁  李江  徐蔚云 《华东地质学院学报》2007,30(3):279-282

变性梯度凝胶电泳技术是目前研究微生物群落遗传多样性及种群动态性有效手段,已被广泛应用于土壤、活性污泥、生物膜、动物肠道、热泉、湖泊等环境微生物生态学研究。阐述了变性梯度凝胶电泳的原理,分析其在微生物多样性分析方面的优越性及局限性;并着重介绍了PCR-DGGE应用过程中的技术发展,包括Cloning/DGGE,GC-DGGE,Nested PCR-DGGE和DG-DGGE。  相似文献   

西藏冈底斯尼木地区斑岩铜矿地质特征与找矿标志   总被引：1，自引：0，他引：1  

晏子贵  李作华 《四川地质学报》2007,27(2):99-103

西藏冈底斯斑岩铜矿带属全球特提斯—喜马拉雅构造—成矿域东延部分,位于冈底斯火山—岩浆弧中东段,为国土资源大调查以来确认的最重要的成矿区带之一。尼木西北部地区为冈底斯斑岩铜矿带的重要组成部分,现已确认为冈底斯成矿带上最重要的斑岩型铜(钼)矿化集中区之一。通过成矿地质背景、典型矿床地质特征、成矿控制因素的综合分析,对尼木地区斑岩铜矿的找矿标志进行了初步总结。  相似文献   

HBV模型在中国东北多冰雪地区的应用研究   总被引：4，自引：0，他引：4  

张建新  赵孟芹  章树安  许珂艳  裴莹 《水文》2007,27(4):31-34

HBV模型是瑞典SMHI开发研制的水文预报模型，广泛用于水文预报、未控制河流的流量模拟、设计洪水计算和水质研究。HBV／IHMS的积雪和融雪模型，可以模拟冰河和田间积雪区的降水量。中国东北地区有较长冰雪覆盖期，春汛预报和春季抗旱水量分析尤为重要，但是缺少考虑融雪（冰）的洪水预报方案和软件系统。利用鸟苏里江一级支流挠力河的历史水文气象资料作为实例应用HBV／IHMS，模拟效果良好，分析结果表明HBV模型对于中国东北多冰雪地区的洪水或水量预报是可行的。  相似文献   

大试管回流消解-等离子体发射光谱／质谱法测定植物样品中多元素   总被引：2，自引：1，他引：2  

马生凤温宏利  巩爱华孙德忠 《岩矿测试》2007,26(3):183-187

采用大试管和红外加热的多孔控温消解炉的简单装置消解植物样品，电感耦合等离子体发射光谱／质谱法测定其中的主量和痕量元素，对消解温度、消解时间、消解溶剂、样品取样量等条件进行考察。用植物国家一级标准物质进行前处理及测定，验证方法的可行性，测定值与标准值基本吻合。方法不仅能同时测定B、Ba、Ca、Cd、Cu、K、Mg、Mn、Na、Ni、P、S、Zn、CoSr、Pb、Cr等元素，还适用于Hg、As挥发性元素的消解测定。方法的精密度（RSD，n=10）≤10％。  相似文献   

云南茶树林铅锌矿电磁波找矿效果     

李本茂  张学良  史家良  普建林 《云南地质》2007,26(2):257-263

茶树林铅锌矿，过去认为震旦系赋矿，钻探验证未见矿。本次利用物探方法实施深部探矿。依据物探异常，结合地质规律综合分析，认为是构造控矿。布置验证坑道，沿断裂破碎带发现新的厚大、品位高铅锌矿体，收到良好的找矿效果。  相似文献   

山东大尹格庄金矿蚀变岩中矿体分布稳定性的动力学控制参量探讨   总被引：2，自引：3，他引：2  

王庆飞邓军  万丽杨立强  龚庆杰 《岩石学报》2007,23(4):861-864

元素品位分布的相依性指标——赫斯特指数(Hurst指数)是矿体分布稳定性的动力学控制参量,重标极差(R/S)分析是相依性分析的有效方法。利用R/S分析对胶东大尹格庄金矿黄铁绢英岩内不同勘探线金元素品位系列的Hurst指数计算发现,Hurst指数主要介于0.59至0.89之间,均值为0.75,标准差为0.09,均大于随机行走经验Hurst指数的均值与标准差;计算结果说明元素品位分布存在正相关性,但由于受多种因素影响,其空间分布的相依性具有一定波动。元素品位分布的正相依性显示在特定的地质背景内,矿体连续分布或者间断出现,发育相对稳定,其与大尹格庄金矿的地质事实相吻合。  相似文献   

Tidally dominated, rimmed-shelf facies of the Picún Leufú Formation (Jurassic/Cretaceous boundary) in southwest Gondwana, Neuquén Basin, Argentina     

Claudia Armella  Nora Cabaleri  Hctor A. Leanza 《Cretaceous Research》2007,28(6):961-979

A palaeoenvironmental model for the Picún Leufú Formation (Jurassic/Cretaceous boundary), which crops out in the Neuquén Basin, Argentina, on the southwestern margin of Gondwana, is presented in this paper for the first time. Detailed stratigraphic sections exposed along National Road 40 where it crosses the Picún Leufú Creek (type locality) and in the Cerrito Caracoles area, were examined and sampled. Based on a combination of the sedimentological data obtained (facies/microfacies analysis) and the relationship between benthic macrofaunas and their taphonomic attributes, it is concluded that the formation reflects a tidally dominated, rimmed-shelf setting characterized by prograding bars dissected by channels and thick lagoonal facies with shoal developments. In the Cerrito Caracoles area, where only the basal part of the formation is exposed, it is interpreted to have been deposited in a shallow subtidal marine environment in which shelf margin facies with patch reefs have been recognized.  相似文献   

Younger Dryas to mid-Holocene environmental history of the lowlands of NW Transylvania, Romania   总被引：2，自引：1，他引：2  

Angelica Feurdean  Volker Mosbrugger  Bogdan P. Onac  Victor Polyak  Daniel Veres   《Quaternary Research》2007,68(3):364-378

Pollen, micro-charcoal and total carbon analyses on sediments from the Turbuta palaeolake, in the Transylvanian Basin of NW Romania, reveal Younger Dryas to mid-Holocene environmental changes. The chronostratigraphy relies on AMS ¹⁴C measurements on organic matter and U/Th TIMS datings of snail shells. Results indicate the presence of Pinus and Betula open woodlands with small populations of Picea, Ulmus, Alnus and Salix before 12,000 cal yr BP. A fairly abrupt replacement of Pinus and Betula by Ulmus-dominated woodlands at ca. 11,900 cal. yr BP likely represents competition effects of vegetation driven by climate warming at the onset of the Holocene. By 11,000 cal yr BP, the woodlands were increasingly diverse and dense with the expansion of Quercus, Fraxinus and Tilia, the establishment of Corylus and the decline of upland herbaceous and shrubs taxa. The marked expansion of Quercus accompanied by Tilia between 10,500 and 8000 cal yr BP could be the result of low effective moisture associated with both low elevation of the site and with regional change towards a drier climate. At 10,000 cal yr BP, Corylus spread across the region, and by 8000 cal yr BP it replaced Quercus as a dominant forest constituent, with only little representation of Picea abies. Carpinus became established around 5500 cal yr BP, but it was only a minor constituent in local woodlands until ca. 5000 cal yr BP. Results from this study also indicate that the woodlands in the lowlands of Turbuta were never closed.  相似文献   

U–Pb SHRIMP monazite ages of the giant Morro Velho and Cuiabá gold deposits, Rio das Velhas greenstone belt, Quadrilátero Ferrífero, Minas Gerais, Brazil     

L.M. Lobato  J.O.S. Santos  N.J. McNaughton  I.R. Fletcher  C.M. Noce 《Ore Geology Reviews》2007,32(3-4):674

U–Pb SHRIMP results of 2672 ± 14 Ma obtained on hydrothermal monazite crystals, from ore samples of the giant Morro Velho and Cuiabá Archean orogenic deposits, represent the first reliable and precise age of gold mineralization associated with the Rio das Velhas greenstone belt evolution, in the Quadrilátero Ferrífero, Brazil. In the basal Nova Lima Group, of the Rio das Velhas greenstone belt, felsic volcanic and volcaniclastic rocks have been dated between 2792 ± 11 and 2751 ± 9 Ma, coeval with the intrusion of syn-tectonic tonalite and granodiorite plutons, and also with the metamorphic overprint of older tonalite–trondhjemite–granodiorite crust. Since cratonization and stable-shelf sedimentation followed intrusion of Neoarchean granites at 2612 + 3/− 2 Ma, it is clear that like other granite–greenstone terranes in the world, gold mineralization is constrained to the latest stages of greenstone evolution.  相似文献   

重庆市沥鼻峡背斜盐井矿区龙潭组沉积特征及聚煤规律   总被引：1，自引：0，他引：1  

邓光明  曾金源 《中国煤田地质》2007,19(6):5-8

通过对重庆市沥鼻峡背斜盐计矿区含煤地层研究，探讨了矿区龙潭组的沉积特征和聚煤规律，得出矿区龙潭组为泻湖-海湾-潮坪沉积体系，成煤时期的不同沉积环境，聚煤沉降幅度小，沉积速度和物质补偿均衡，有利于泥炭堆积，因此矿区含煤层数、煤层厚度较稳定，该区处于华蓥山潮坪地带，是龙潭期聚煤中心之一。  相似文献