一株胶质芽胞杆菌对磷矿石风化作用的实验研究   总被引：1，自引：0，他引：1  

谌书  连宾  刘丛强 《矿物学报》2008,28(1):77-83

以一株质芽孢杆菌为例研究实验条件下微生物对磷矿石的风化作用.以直接作用和间接作用的方式研究培养基中胶质芽孢杆菌对磷矿粉的风化作用,即在装有100目磷矿石粉的液体培养基中接种,研究该菌对磷矿石粉的直接风化作用;同时,将装有100目磷矿石粉的透析袋放入液体培养基中再接入该菌,研究其对磷矿石粉的间接风化作用.按不同时间取培养液上清液,过滤,用电感耦合等离子体发射光谱(ICP-OES)测定滤液中Ca2 、Mg2 、Na 、Mn2 、Al3 、Fe3 和K 等浓度,比色法测定水溶性P(Pws)和水溶性Si (Siws) 的含量;滤膜上的固体物称重并消解后,同上方法测定Ca2 、Mg2 、Na 、Mn2 、Al3 、Fe3 和K 等浓度以及Pws和Siws含量.此外,细菌风化作用后的矿物残渣用电子探针作表面微观形态分析和XRD矿物物相分析.结果表明:胶质芽孢杆菌对磷矿石粉风化的直接作用强度大于间接作用;对不同矿物的风化强度不同,对粘土矿物的风化作用较明显.提出细菌对磷矿石的风化作用源自细菌生长导致的机械破坏作用、胞外分泌物的生化降解作用以及多种因素之间的协同作用.  相似文献   

Simulating a heavy metal spill under estuarine conditions: Effects on the clam Scrobicularia plana     

E. García-Luque  T. A. DelValls  C. Casado-Martínez  J. M. Forja  A. Gmez-Parra 《Marine environmental research》2004,58(2-5):671

We describe the effect of heavy metals Zn, Cd, Pb and Cu on the induction of methallothioneins on the clam Scrobicularia plana along a salinity gradient simulated under laboratory conditions. The clams were exposed to constant heavy metal concentrations in a dynamic estuary simulator during a 15-day assay to investigate possible induction of metal-binding proteins in them. The concentration of heavy metals in water was analysed. Clams were analysed for methallothionein concentrations. The speciation of Zn, Cd, Pb and Cu along the salinity gradient was modelled. Zn showed the highest concentrations and its prevalent species was the free ion. Intersite differences have been observed in methallothionein concentration and related to the salinity gradient. It seems that synthesis of methallothioneins is the result of physiological forces acting in concert with the changes in the chemical speciation of metals, owing to the trace metals uptake is controlled by means of an interaction of physiology and physicochemistry.  相似文献   

大窑湾海域水化要素分布及相关关系的探讨     

邵秘华  孙茜  贺广凯  张国光  刘彤 《海洋科学进展》1995,(2)

本文依据大窑湾的环境调查资料为基础，系统地阐述了水体中化学要素ｐＨ、盐度、透明度、ＤＯ、ＣＯＤ、ＴＯＣ、浊度、悬浮物、ＮＨ４－Ｎ、ＮＯ２－Ｎ、ＮＯ３－Ｎ、ＰＯ４－Ｐ、ＳｉＯ３－Ｓｉ、Ｃｕ、Ｐｂ、Ｚｎ、Ｃｄ、Ａｓ、Ｈｇ、Ｃｒ、硫化物、六六六、石油等分布规律。诸要素主要是在潮流水动力支配下，形成扩散带。各要素中仅有无机氮和ＰＯ４－Ｐ两项含量超标，其余要素含量范围都是处于海水正常浓度水平，属于一类海水水质。文章并对各要素进行相关关系分析。  相似文献   

藻体海水培养液中铜的化学存在形式     

于圣睿 《中国海洋大学学报(自然科学版)》1990,(4)

采用金属络合物通式模型,在IBM-PC计算机上计算了不同pH(7.5～9.5)、碱度(2.3～2.5mmol dm~(-3))和加入不同浓度的铜离子(1.0×10~(-4)～1.0mg dm~(-3))条件下,海水培养液中铜的主要化学存在形式分配的百分数。还给出了计算机程序框图和主要化学存在形式含量的百分数随pH、碱度和铜浓度变化图。  相似文献   

Effects of chemical ecological adjustment and control experiment on phytoplankton community in the Aoshan Bay     

Chen Bijuan  Zhao Jun  Xin Fuyan  Cui Yi  Guo Feng 《海洋学报(英文版)》2002,21(1):115-123

There is a low nutrient level in the Aoshan Bay. In June 1999, the chemical adjustmentand control experiment was made in the Aoshan Bay. Following tracts investigation was carried out before the experiment and on the 1st, 2nd, 4th, 5th, 6th and 45th day/after the experiment. While the variance of amount of phytoplankton, the replacement of superior species and the species composition of phytoplankton were researched. The results show that the amount of phytoplankton in the Aoshan Bay rises gradually after the experiment. Ceratium macroceros Cleve of pyrophyta was the dominant species before the experiment, its dominant index was 37.7％. Six days after the experiment, its dominant index dropped to 17.6％ . Meanwhile the dominant index of Asterionella japanics Cleve rose from 7.1％ to 39.2％ , it became the first dominant species. Forty-five days after the experiment, the amount of phytoplankton in the Aoshan Bay was 5.15 to 137.32 times more than that in 1997.  相似文献   

Variability in the nutritional value of the major copepods in Cape Cod Bay (Massachusetts, USA) with implications for right whales     

Amy DeLorenzo Costa  Edward G. Durbin  & Charles A. Mayo 《Marine Ecology》2006,27(2):109-123

The North Atlantic right whale, a seriously endangered species, is found in Cape Cod Bay (Massachusetts, USA) during the winter and early spring. During their residency in these waters, these whales are frequently observed feeding. This study evaluated spatial and temporal changes in the chemical composition (carbon weight and C/N ratio) of the food resource targeted by the right whales in Cape Cod Bay. The three taxa measured (Centropages typicus, Pseudocalanus spp., and Calanus finmarchicus) had highly variable chemical compositions resulting from the different life strategies and from fluctuations in their surrounding environment. The impact of seasonal variability in the energy densities of the food resource of right whales was calculated and compared to the energetic requirements of these whales. Calculations indicated that differences in the nutritional content of the zooplankton prey in Cape Cod Bay could have a considerable effect on the nutrition available to the right whales. Therefore, it is likely that using more precise estimates of the energetic densities of the prey of right whales would lead to a re‐evaluation of the adequacy of the food resource available to these whales in the North Atlantic.  相似文献   

翅碱蓬(Suaeda heteroptera)根际与非根际沉积物常见重金属总量及化学形态变化     

朱鸣鹤  丁永生  丁德文 《海洋与湖沼》2006,37(5):393-400

原子吸收光谱法和等离子光谱法分别研究了潮滩盐沼植物翅碱蓬(Suaedaheterop-tera)根际与非根际(根上部和根下部)沉积物中Cu、Zn、Pb和Cd的总量和化学形态。结果表明,从总量来看,不同潮滩沉积物中4种重金属次序均为Zn>Pb>Cu>Cd,但同一元素随潮滩位置变化明显,尤以中潮滩差异最显著,重金属总量明显大于其他两个潮滩(低潮滩和高潮滩),特别是根际沉积物中的重金属总量远高于非根际沉积物总量,其比值分别为Cu3·4—4·2倍,Zn2·2—2·7倍,Pb3·2—3·3倍。同一潮滩均表现为根际沉积物>根上部>根下部。Cd含量相对较低,其变化不明显。从化学形态看,沉积物重金属表现为环境直接影响态(交换态和有机结合态)、环境间接影响态(碳酸盐态和铁锰氧化物态)和稳定态(残渣态)。与非根际沉积物相比,根际沉积物中重金属的化学形态发生了显著的变化,Cu和Pb以稳定态为主,其次为环境间接影响态,环境直接影响态最低;Zn与上述2种金属不同,以环境直接影响态含量最高(可交换态是有机结合态的7倍),其次分别为环境间接影响态(主要是碳酸盐结合态)和稳定态。实验结果表明,由于特异根圈效应,一方面该植物使可迁移形态的Cu和Pb在根际逐步得到矿化,使其生物可利用性降低;另一方面使Zn的生物可利用性提高,促进了植物对Zn的吸收利用。  相似文献   

刍论大连湾底质地化环境   总被引：6，自引：0，他引：6  

鲍永恩  马嘉蕊 《海洋科学》1990,(4)

本文根据1987年的调查资料,总结阐述了大连湾底质地化环境诸要素的分布规律及其特征,运用相关分析法,通过多元回归计算求得了诸要素之间的关系。经过综合因素分析,得出了大连湾氧化还原环境分区、还原环境的成因及发展趋势。  相似文献   

Complexation of mercury by dissolved organic matter in surface waters of Galveston Bay, Texas   总被引：3，自引：0，他引：3  

Seunghee Han  Gary A. Gill  Ronald D. Lehman  Key-Young Choe 《Marine Chemistry》2006,98(2-4):156-166

The chemical speciation of dissolved mercury in surface waters of Galveston Bay was determined using the concentrations of mercury-complexing ligands and conditional stability constants of mercury-ligand complexes. Two classes of natural ligands associated with dissolved organic matter were determined by a competitive ligand exchange-solvent solvent extraction (CLE-SSE) method: a strong class (L_s), ranging from 19 to 93 pM with an average conditional stability constant (K_HgLs) of 10²⁸, and a weak class (L_w) ranging from 1.4 to 9.8 nM with an average K_HgLs of 10²³. The range of conditional stability constants between mercury and natural ligands suggested that sulfides and thiolates are important binding sites for dissolved mercury in estuarine waters. A positive correlation between the estuarine distribution of dissolved glutathione and that of mercury-complexing ligands supported this suggestion. Thermodynamic equilibrium modeling using stability constants for HgL, HgCl_x, Hg(OH)_x, and HgCl(OH) and concentrations of each ligand demonstrated that almost all of the dissolved mercury (> 99%) in Galveston Bay was complexed by natural ligands associated with dissolved organic matter. The importance of low concentrations of high-affinity ligands that may originate in the biological system (i.e., glutathione and phytochelatin) suggests that the greater portion of bulk dissolved organic matter may not be important for mercury complexation in estuarine surface waters.  相似文献   

坛紫菜中藻胆蛋白的性质与化学组成研究   总被引：10，自引：1，他引：10       下载免费PDF全文

高洪峰  曹文达  纪明侯 《海洋与湖沼》1993,24(4):350-355

坛紫菜(于1988年采自青岛太平角海区人工养殖筏上)的水溶性色素粗提物经过硫酸铵沉淀和羟基磷灰石(HA)柱层析后,分离出藻蓝蛋白(RPC)、藻红蛋白(RPE)和变藻蓝蛋白(APC)。在中性介质中,其吸收光谱和荧光发射光谱与文献报道基本一致;但在酸性(pH=3)或碱性(pH=12)介质中,吸收光谱较在中性介质中有明显改变,原有的荧光性质也消失。RPC和APC只分离到一种聚集体,但RPE有两种不同的聚集体。用SephadexG—100凝胶过滤方法测量藻胆蛋白的分子量分别为:RPC117000,APC112000,小分子RPE38000,大分子RPE232000。对三种藻胆蛋白的氨基酸分析的结果表明,三种藻胆蛋白中都是酸性氨基酸的含量大于碱性氨基酸的含量。  相似文献