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91.
研究肝素酶Ⅰ(来源于Flavobacterumheparinase,EC4.2.2.7)对人工合成的且含有与抗凝血酶Ⅲ特定结合位点的肝素五糖(SHP)的酶解作用,并对酶解作用的动力学进行研究。利用强阴离子高效液相色谱(SAX-HPLC)对酶解混合物进行分离,利用质谱(ESIMS)和核磁共振波谱(1H-NMR)技术对得到的二糖和三糖的结构进行确证。研究结果表明,这种被作为肝素反向试剂的肝素酶Ⅰ可水解人工所合成的肝素五糖,从而使之丧失抗Xa因子活性。 相似文献
92.
海洋木栖真菌抗菌活性的初步研究 总被引:6,自引:0,他引:6
从福建厦门海沧、集美及漳州浮宫等地采集到的红树林等海洋潮间带的各种腐木样品中分离得到176株海洋木栖真菌,对其进行抗菌活性检测.其结果表明共有96株海洋木栖真菌对大肠杆菌、枯草杆菌及白色假丝酵母中的一种或多种拮抗指示菌具有抑制作用,占供测菌株总数的54.5%.在这些活性菌株中,红树源菌株拮抗比例为30.2%,非红树源菌株拮抗比例为69.8%.具有抗菌活性的菌株主要分布于15个属中,包括木霉Trichoderma、青霉Penicillium、拟青霉Paecilomyces和一些不产孢的分类群.某些稀有真菌如盘多毛孢Pestalotia、茎点霉Phoma也具有一定的抗菌活性. 相似文献
93.
94.
Takushi Niki Miwa Shimizu Ayako Fujishiro Junji Kinoshita 《Journal of Oceanography》2007,63(5):873-877
During time-series observations in Sagami Bay, Japan, the concentration of dissolved dimethylsulfoniopropionate (DMSPd), a precursor of dimethylsulfide (DMS), was negatively correlated with salinity. In the laboratory, low-salinity shock reduced
DMS production rates of the natural bacterial community and induced rapid DMSP release from a dinophyte, Heterocapsa triquetra, suggesting that low-salinity shock reduced DMSPd consumption but enhanced DMSPd production, which agrees with the negative correlation between DMSPd and salinity observed in Sagami bay. In addition, low-salinity shock did not affect DMSP lyase activity of H. triquetra. Low-salinity shock would increase the contribution from algae in DMS production, leading to an increase in potential DMS
productivity in the environment. 相似文献
95.
Takushi?NikiEmail author Taiki?Fujinaga Mariyo?F.?Watanabe Junji?Kinoshita 《Journal of Oceanography》2004,60(5):913-917
Solid-phase microextraction (SPME) is a simple, sensitive and less destructive method for the determination of dimethylsulfide
(DMS) in seawater. Combined with detection by gas chromatography-mass spectrometry (GC-MS), the method had sufficient sensitivity
(minimum detectable concentration of DMS was 0.05 nM), and practical levels of reproducibility (relative standard deviation
≤7%) and linearity (r
2 > 0.995) over a wide concentration range (0.5 to 910 nM). The protocol developed was applied to a Sagami Bay water sample
to determine concentrations of DMS and DMSP, and in situ DMSP-lyase activity. 相似文献
96.
钝顶螺旋藻藻蓝蛋白和多糖的抗肿瘤免疫活性研究 总被引:14,自引:2,他引:14
对钝顶螺旋藻藻蓝蛋白 (PC)和多糖 (PSP)的抗肿瘤免疫活性进行了研究。采用免疫酶标技术、MTT法及定量溶血分光光度测定法等免疫分析实验 ,从免疫器官、免疫细胞、免疫分子 3个水平上进行藻蓝蛋白和多糖的抗肿瘤免疫活性检测。结果显示 ,藻蓝蛋白和多糖处理组小鼠瘤块直径及瘤体重量均小于对照组 ,T细胞及 B细胞活性明显增强 ,体液抗体量显著提高 ,螺旋藻藻蓝蛋白比多糖抑制肿瘤细胞生长和提高机体免疫力的作用更明显。 相似文献
97.
乙醇对文昌鱼酸性磷酸酶活力与构象的影响 总被引:1,自引:0,他引:1
文昌鱼酸性磷酸酶是一种含有铁离子的金属酶,酶的紫外-可见光谱扫描,280nm,500nm有最大吸收峰,320nm有肩峰。荧光扫描:在281nm激发下,330nm有发射峰,在480nm激发下,515nm有一发射峰。 相似文献
98.
From the phase-resolving improved Boussinesq equations (Beji and Nadaoka, Ocean Engineering 23 (1996) 691), a phase-averaged Boussinesq model for water waves is derived by more effectively describing carrier wave groups and accompanying long wave evolution with less CPU time. Linear shoaling characteristics of carrier wave equations are investigated and found to agree exactly with the analytical expression obtained from the constancy of energy flux for the improved Boussinesq equations themselves, showing that the present model equations are the results of a consistent derivation procedure regarding energy considerations. Numerical simulations of the derived equations for the single wave group and narrow-banded random waves show the validity of the present model and its high performance, especially on the CPU time. 相似文献
99.
Under artificial LD cycles(6,12,18 L),the elvers of Japanese eel,Anguilla japonica,showed a 24 h cycle of locomotor activity rhythm being most active at light transitions:the eels' activity rose to a primary peak after lights-off,followed by a quiescent period during which they buried into the shelters or lying motionlessly on sand for most of the time,and then reached a secondary peak before lights-on.Elvers could resynchronize their activity rhythm with a new photo cycle within 4 d.Moreover,their activity level at dark phase significantly increased as the light period was prolonged:higher activity levels during shorter dark period.However,the elvers did not display clearly the existence of a circadian rhythm under constant light or dark conditions.The timing of daily activity rhythm evidenced in the Japanese eels may occur through the action of the LD cycles with a weak participation of an endogenous circadian system.In all the LD cycles,over 99% of the activity occurred in the dark phase,indicating that the eels were always nocturnally active no matter what time of day it might be.Under 12 L conditions,the eels' activity level and the time outside sand were significantly elevated both at light and dark phases as temperature increased from 10~15 to 20~25 ℃.The activity rhythm pattern(i.e.,two peaks occurring around light transitions) did not apparently change among temperatures.However,in contrast with the primary activity peaks immediately after lights-off at 20 and 25 ℃,the timing of the primary peaks at 10 and 15 ℃ showed a latency of a few hours following lights-off,indicating the inhibiting effect of low temperature on the eels' activity. 相似文献
100.
Hydrodynamic modeling of flushing time in a small estuary of North Bay, Florida, USA 总被引:1,自引:0,他引:1
Freshwater fraction method is popular for cost-effective estimations of estuarine flushing time in response to freshwater inputs. However, due to the spatial variations of salinity, it is usually expensive to directly estimate the long-term freshwater fraction in the estuary from field observations. This paper presents the application of the 3D hydrodynamic model to estimate the distributions of salinity and thus the freshwater fractions for flushing time estimation. For a case study in a small estuary of the North Bay in Florida, USA, the hydrodynamic model was calibrated and verified using available field observations. Freshwater fractions in the estuary were determined by integrating freshwater fractions in model grids for the calculation of flushing time. The flushing time in the North Bay is calculated by the volume of freshwater fraction divided by the freshwater inflow, which is about 2.2 days under averaged flow conditions. Based on model simulations for a time series of freshwater inputs over a 2-year period, a power regression equation has been derived from model simulations to correlate estuarine flushing time to freshwater inputs. For freshwater input varying from 12 m3/s to 50 m3/s, flushing time in this small estuary of North Bay changes from 3.7 days to 1.8 days. In supporting estuarine management, the model can be used to examine the effects of upstream freshwater withdraw on estuarine salinity and flushing time. 相似文献