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Spatial (10 different locations) and temporal (2 years) changes in characteristics of the Marmara Sea Sediments were monitored to determine interactions between the chemical and microbial diversity. The sediments were rich in terms of hydrocarbon, nitrate, Ni and microbial cell content. Denitrifying, sulfate reducing, fermentative and methanogenic organisms were co-abundant in 15 cm below the sea floor. The local variations in the sediments’ characteristics were more distinctive than the temporal ones. The sulfate and nitrate contents were the main drivers of the changes in the microbial community compositions. N and P were limited for microbial growth in the sediments, and their levels determined the total cell abundance and activity. Seasonal shifts in temperatures of the shallow sediments were also reflected in the active cell abundances. It was concluded that the Marmara Sea is a promising ecosystem for the further investigation of the ecologically important microbial processes. 相似文献
34.
应用孤岛油田现场聚合物和美国进口复合有机铬交联剂TIORCOWC684进行了一系列的室内评价实验。实验结果表明,宝莫聚合物均具有较好的初始黏度、且形成的弱冻胶体系强度高;弱冻胶主段塞选取聚合物浓度2500--3000mg/L,交联剂浓度300--600mg/L,成胶强度较高,且胶体不易脱水;弱冻胶封口段塞聚合物浓度为4000mg/L,交联剂浓度300--900mg/L,胶体强度较大、韧性好,满足封口的要求;同时评价了封口段塞的耐盐性能,结果表明该体系随盐类矿化度的增加体系强度有增加趋势。 相似文献
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X. Huang Y. Tian Y.R. Luo H.J. Liu Wei Zheng T.L. Zheng 《Marine pollution bulletin》2008,57(6-12):538
Sublimation was developed by Alley and Brown (2000) in order to isolate bacterial strains that were capable of degrading water insoluble compounds. In this study, sublimation was modified by the use of nutritional agar plates, instead of mineral salt agar, to isolate phenanthrene-degrading bacteria from a mixed culture that had been enriched under the selective pressure of high phenanthrene content. Five strains were obtained with different morphology and degradation ability. Based on the 16S rDNA sequence, two of them were classified as species of the genus Sphingomonas; the others as species of the genus Burkholderia. Denaturing gradient gel electrophoresis (DGGE) was introduced to detect dynamic changes in the bacterial community during enrichment batch culture, and to determine any correlation between the five isolates and the phenanthrene-degrading consortium. The DGGE profile indicated that these five isolates corresponded to four dominant bands of the consortium. Compared to traditional means of isolation, we concluded that modified sublimation is effective and more convenient. 相似文献
37.
YE Ying JI Shanshan WU Daidai LI Jun ZHANG Weirui 《《地质学报》英文版》2006,80(2):200-205
The absorption reactions of arsenite on Fe (hydro-)oxides are studied. The three absorbent types are Fe(OH)3 gel and two Fe (hydro-)oxides, in which the Fe(OH)3 gel was dried in a microwave oven under vacuum at 80℃. It is found that pH changes from 9.71 to 10.36 in 6 minutes after the Fe (OH)3 gel was mixed with NaAsO2 solution, as the arsenite replaces the OH- in goethite and Fe(OH)3. At the 40th minute after the start of the reaction, pH decreases, which is most probably because that the monodentate surface complex of absorbed arsenite has changed into mononuclear-bidentate complex and released proton. The decline in pH values indicates not the end of the absorption but a change in the reaction type. Temperature and dissolved gas has little effect on these two types of reactions. The total absorption of arsenite increases after the absorbent is irradiated with ultrasound, which also lead to difficulty in separating the solids from solution. The absorption capacity for arsenite of Fe(OH)3 gel dried in a microwave oven under vacuum is 53.18% and 17.22% respectively better than that of Fe (OH)3 gel and gel dried at 80℃. The possible reasons are that the water molecules in the gel vibrates with high frequency under the effect of microwave irradiation, thereby producing higher porosity and improved surface activity. 相似文献
38.
海域浅层气地质调查和盐湖环境科学钻探在松散砂岩钻探取样工程中,存在孔壁易坍塌、成孔率低等技术难题,为此开展了松散砂岩地层环保型弱凝胶护壁护心钻井液技术研究。通过弱凝胶结构研究及成胶剂、交联剂及抗盐降滤失剂等材料优选,研发了一套环保型弱凝胶护壁护心钻井液,评价了钻井液的流变性、滤失性、抑制性、护心性能、环保性能等性能,并在山西某盐湖环境科学钻探工程进行现场应用。室内及现场应用结果表明:研制的弱凝胶护壁护心钻井液具有良好的流变性、降滤失性、抑制性(岩屑回收率96.3%)、护壁护心性能及环保性能(BOD5/COD比值24.6%,EC50值47950 mg/L),能有效解决松散砂岩类地层孔壁坍塌、岩屑不易携带等问题,在松散地层钻探工程中具有广阔的应用前景。 相似文献
39.
Interaction of freshly precipitated silica gel with aqueous solutions was studied at laboratory batch experiments under ambient
and near neutral pH-conditions. The overall process showed excellent reversibility: gel growth could be considered as an opposite
process to dissolution and a linear rate law could be applied to experimental data. Depending on the used rate law form, the
resulting rate constants were sensitive to errors in parameters/variables such as gel surface area, equilibrium constants,
Si-fluxes, and reaction quotients. The application of an Integrated Exponential Model appeared to be the best approach for
dissolution data evaluation. It yielded the rate constants k
dissol ∼ (4.50 ± 0.68) × 10−12 and k
growth ∼ (2.58 ± 0.39) × 10−9 mol m−2 s−1 for zero ionic strength. In contrast, a Differential Model gave best results for growth data modeling. It yielded the rate
constants k
dissol ∼ (1.14 ± 0.44) × 10−11 and k
growth ∼ (6.08 ± 2.37) × 10−9 mol m−2 s−1 for higher ionic strength (I ∼ 0.04 to 0.11 mol L−1). The found silica gel solubility at zero ionic strength was somewhat lower than the generally accepted value. Based on the
and standard Gibbs free energy of silica gel formation was calculated as and −850,318 ± 20 J mol−1, respectively. Activation energies for silica gel dissolution and growth were determined as and respectively. An universal value for growth of any silica polymorph, is not consistent with the value for silica gel growth, which questions the hypothesis about one unique activated complex
controlling the silica polymorph growth. 相似文献
40.
Comparative proteome analysis of butachlor-degrading bacteria 总被引:1,自引:0,他引:1
A Pseudomonas putida strain, named ER1, was isolated from an agricultural soil and found to actively degrade the herbicide butachlor. The enzyme
extracted from ER1 could degrade butachlor. Furthermore, incubation of ER1 in a medium containing 50 mg/kg of butachlor after
3 days resulted in the high butachlor-degrading enzyme activity of ER1. Response of ER1 to butachlor might be related to changes
in protein composition at both quantitative and qualitative levels. Total proteins were extracted from control strain (incubated
in the medium without butachlor) and the treated strain (incubated in the medium with butachlor). The proteins were separated
by two-dimensional gel electrophoresis. Of the total number of ER1 protein, 11 spots were significantly changed under butachlor
stress. Analysis by matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry coupled with
database searching allowed the function of some proteins which were similar to the hydrolases activity or oxidoreductase activity. 相似文献