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81.
蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析 总被引:4,自引:0,他引:4
利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌(Coelomactra antiquata)、中国蛤蜊(Mactra chinensis)和四角蛤蜊(Mactra veneriformis)3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列.测序后用DNAstar软件分析了核苷酸差异。结果显示:三种贝类16SrRNA基因片段长度相同,均为306bp(去除引物).核苷酸存在多态性。共有45个变异位点,54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88.9%.与四角蛤蜊的同源性为88.6%.中国蛤蜊与四角蛤蜊的同源性为90.6%。三种蛤蜊ITS2序列分别为390bp(西施舌)、441bp(四角蛤蜊)和466bp(中国蛤蜊)。存在长度多态性.ITS2核苷酸差异分析结果显示.西施舌与中国蛤蜊的同源性为70.9%-71.1%,西施舌与四角蛤蜊的为70.5%-71.0%。中国蛤蜊与四角蛤蜊的同源性为88.1%-88.8%。ITS2序列分析结果与16SrRNA基因片段分析结果一致.2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。 相似文献
82.
Flavio Scappini Cesare Cecchi-Pestellini Harvey Smith William Klemperer Alexander Dalgarno 《Monthly notices of the Royal Astronomical Society》2003,341(2):657-661
We consider sulphur depletion in dense molecular clouds, and suggest hydrated sulphuric acid, H2 SO4 · n H2 O, as a component of interstellar dust in icy mantles. We discuss the formation of hydrated sulphuric acid in collapsing clouds and its instability in heated regions in terms of the existing hot core models and observations. We also show that some features of the infrared spectrum of hydrated sulphuric acid have correspondence in the observed spectra of young stellar objects. 相似文献
83.
84.
Dirk Pandel France A. Córdova Steve B. Howell 《Monthly notices of the Royal Astronomical Society》2003,346(4):1231-1241
We present an analysis of X-ray and ultraviolet (UV) data of the dwarf nova VW Hyi that were obtained with XMM–Newton during the quiescent state. The X-ray spectrum indicates the presence of an optically thin plasma in the boundary layer that cools as it settles on to the white dwarf. The plasma has a continuous temperature distribution that is well described by a power law or a cooling flow model with a maximum temperature of 6–8 keV. We estimate from the X-ray spectrum a boundary layer luminosity of 8 × 1030 erg s-1 , which is only 20 per cent of the disc luminosity. The rate of accretion on to the white dwarf is 5 × 10−12 M⊙ yr−1 , about half of the rate in the disc. From the high-resolution X-ray spectra, we estimate that the X-ray emitting part of the boundary layer is rotating with a velocity of 540 km s−1 , which is close to the rotation velocity of the white dwarf but is significantly smaller than the Keplerian velocity. We detect a 60-s quasi-periodic oscillation of the X-ray flux, which is likely to be due to the rotation of the boundary layer. The X-ray and the UV flux show strong variability on a time-scale of ∼1500 s. We find that the variability in the two bands is correlated and that the X-ray fluctuations are delayed by ∼100 s. The correlation indicates that the variable UV flux is emitted near the transition region between the disc and the boundary layer and that accretion rate fluctuations in this region are propagated to the X-ray emitting part of the boundary layer within ∼100 s. An orbital modulation of the X-ray flux suggests that the inner accretion disc is tilted with respect to the orbital plane. The elemental abundances in the boundary layer are close to their solar values. 相似文献
85.
MENGLi-li 《中国地理科学(英文版)》2003,13(2):190-192
The exploitation and utilization of the tour resources of tournament athletics, including skiling, boat sail-ing, archery, ice engraving, snow engraving, has become a new trend of the development of Chinese tourism. Due tothe unique cold climate and superior geographic location, Harbin is a promising city for developing tour resources of tourna-ment athletics. Based on the analysis of the superiority and peculiarity of Harbin, the speculation on development of tour resources of tournament athletics in Harbin is proposed as follows : 1 ) Harbin should develop its special tour resources of tournament athletics associated with needs of market; 2) Harbin should take the advantages of rich resources and devel-op ice and snow entertainment in winter and travel for sight-seeing and spending summer;, 3) the adjustment of the layoutof ice and snow resources should be based on the idea of taking Harbin as the center and all-side opening at the largescale in the way of radiation; 4) tourism should be developed by the combined efforts of various departments to make feasi-ble plan, and the organizers should pay much attention to ensuring the safetv of tourists. 相似文献
86.
87.
Moche Ziv 《国际地质力学数值与分析法杂志》2003,27(3):207-232
The response of an ideal elastic half‐space to a line‐concentrated impulsive vector shear force applied momentarily is obtained by an analytical–numerical computational method based on the theory of characteristics in conjunction with kinematical relations derived across surfaces of strong discontinuities. The shear force is concentrated along an infinite line, drawn on the surface of the half‐space, while being normal to that line as well as to the axis of symmetry of the half‐space. An exact loading model is introduced and built into the computational method for this shear force. With this model, a compatibility exists among the prescribed applied force, the geometric decay of the shear stress component at the precursor shear wave, and the boundary conditions of the half‐space; in this sense, the source configuration is exact. For the transient boundary‐value problem described above, a wave characteristics formulation is presented, where its differential equations are extended to allow for strong discontinuities which occur in the material motion of the half‐space. A numerical integration of these extended differential equations is then carried out in a three‐dimensional spatiotemporal wavegrid formed by the Cartesian bicharacteristic curves of the wave characteristics formulation. This work is devoted to the construction of the computational method and to the concepts involved therein, whereas the interpretation of the resultant transient deformation of the half‐space is presented in a subsequent paper. Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
88.
A discrete element modelling of bonded granulates and investigation on the bond effect on their behaviour are very important to geomechanics. This paper presents a two‐dimensional (2‐D) discrete element theory for bonded granulates with bond rolling resistance and provides a numerical investigation into the effect of bond rolling resistance on the yielding of bonded granulates. The model consists of mechanical contact models and equations governing the motion of bonded particles. The key point of the theory is that the assumption in the original bond contact model previously proposed by the authors (55th CSCE‐ASCE Conference, Hamilton, Ont., Canada, 2002; 313–320; J. Eng. Mech. (ASCE) 2005; 131 (11):1209–1213) that bonded particles are in contact at discrete points, is here replaced by a more reliable assumption that bonded particles are in contact over a width. By making the idealization that the bond contact width is continuously distributed with the normal/tangential basic elements (BE) (each BE is composed of spring, dashpot, bond, slider or divider), we establish a bond rolling contact model together with bond normal/tangential contact models, and also relate the governing equations to local equilibrium. Only one physical parameter β needs to be introduced in the theory in comparison to the original bond discrete element model. The model has been implemented into a 2‐D distinct element method code, NS2D. Using the NS2D, a total of 86 1‐D, constant stress ratio, and biaxial compressions tests have been carried out on the bonded granular samples of different densities, bonding strengths and rolling resistances. The numerical results show that: (i) the new theory predicts a larger internal friction angle, a larger yielding stress, more brittle behaviour and larger final broken contact ratio than the original bond model; (ii) the yielding stress increases nonlinearly with the increasing value of β, and (iii) the first‐yield curve (initiation of bond breakage), which define a zone of none bond breakage and which shape and size are affected by the material density, is amplified by the bond rolling resistance in analogous to that predicted by the original bond model. Copyright © 2006 John Wiley & Sons, Ltd. 相似文献
89.
It is well known that the Babuska–Brezzi stability criterion or the Zienkiewicz–Taylor patch test precludes the use of the finite elements with the same low order of interpolation for displacement and pore pressure in the nearly incompressible and undrained cases, unless some stabilization techniques are introduced for dynamic analysis of saturated porous medium where coupling occurs between the displacement of solid skeleton and pore pressure. The numerical manifold method (NMM), where the interpolation of displacement and pressure can be determined independently in an element for the solution of u–p formulation, is derived based on triangular mesh for the requirement of high accurate calculations from practical applications in the dynamic analysis of saturated porous materials. The matrices of equilibrium equations for the second‐order displacement and the first‐order pressure manifold method are given in detail for program coding. By close comparison with widely used finite element method, the NMM presents good stability for the coupling problems, particularly in the nearly incompressible and undrained cases. Numerical examples are given to illustrate the validity and stability of the manifold element developed. Copyright © 2006 John Wiley & Sons, Ltd. 相似文献
90.