Particulate peptidoglycan in seawater determined by the silkworm larvae plasma (SLP) assay     

Akira Shibata  Kouichi Ohwada  Masakazu Tsuchiya  Kazuhiro Kogure 《Journal of Oceanography》2006,62(1):91-97

Peptidoglycan (PG) is a biopolymer found exclusively in the cell wall of bacteria. Recent chemical analysis of particulate organic matter suggests that a major amount of the muramic acid, an amino sugar present only in PG, could not be accounted for in terms of bacterial cells (Benner and Kaiser, 2003); however, data on particulate PG is quite sparse. In the present study, conducted in 1996, the PG was examined at 5 sampling sites in the northwestern Pacific Ocean, and in natural seawater cultures. Particulate PG, which was concentrated using a 96-well filtration plate equipped with Durapore filters (pore size, 0.22 μm), was measured by the silkworm larvae plasma (SLP) assay. The PG concentration generally decreased with depth and correlated significantly with bacterial abundance throughout the entire water column. However, the ratio of particulate PG to bacterial abundance varied with depth. The average ratio was 0.61 ± 0.53 (average ± SD, n = 40) between 50 and 2000 m, which agreed with the bacterial cellular PG content from 0.63 to 1.1 fg cell⁻¹ obtained in seawater cultures. On the other hand, the ratios of PG to bacteria from the surface to 50 m (3.7 ± 2.6, n = 29) and below 2,000 m (2.1 ± 1.7, n = 7) were significantly higher than that between 50 and 2,000 m. These results may suggest that, in the surface and deep layers, a significant fraction of particulate PG was present in bacterial detritus, whereas this fraction was reduced in the middle layer.  相似文献   

White-light promoted degradation of leucopterin and related pteridines dissolved in seawater, with evidence for involvement of complexation from major divalent cations of seawater     

Arthur F. Landymore  Naval J. Antia 《Marine Chemistry》1978,6(4):309-325

The photochemical instability of several related pteridines in seawater was investigated by aseptic incubation of solutions at 20–22°C under illumination from cool-white light of intensity 6 kerg cm⁻² sec⁻¹, and the chemical changes were spectrophotometrically monitored. All the pteridines showed markedly accelerated degradation from this illumination relative to their behaviour in total darkness.Pterin and lumazine were degraded very slowly with zero-order reaction kinetics, while the other pteridines photolysed rapidly (according to first-order kinetics) with decomposition rates increasing in the order dioxylumazine (2,4,6,7-tetrahydroxypteridine) < leucopterin < isoxanthopterin < xanthopterin < oxylumazine (2,4,6-trihydroxypteridine). Excepting leucopterin and dioxylumazine, the photolysis rates were attributable to the pH of seawater and not its salt content; this was also the case with oxylumazine which required the salt content of seawater for decomposition in darkness. Leucopterin and dioxylumazine (both 6,7-dihydroxylated pteridines) gave evidence of complexation with the major divalent cations (Ca²⁺, Mg²⁺) of seawater, by virtue of which their photolytic degradation rates were enhanced to magnitudes obtained in pH-10 buffer without seawater. It is proposed that such complexation produces structural forms of these pteridines analogous to their normal ionic forms at pH 10–12.The photolysis of the 6-hydroxylated pteridines (xanthopterin, oxylumazine) proceeded via intermediate formation of their corresponding 7-hydroxylated derivatives (leucopterin, dioxylumazine).  相似文献   

双层局部开孔板沉箱对波浪反射的理论研究   总被引：1，自引：0，他引：1  

李玉成  刘洪杰滕斌 《海洋工程》2005,23(1):18-27,32

提出了一种用于研究由双层开孔板和一个不透水后板的开孔结构对斜向波反射率的理论分析方法。整个流域被分成三个子域，在每个子域内应用特征函数展开法以得到该域内包含未知展开系数的势函数的表达式，在速度势的展开中，考虑了非传播模态波浪的影响。通过匹配开孔板处的边界条件可以求解待定的展开系数，继而求解双层开孔板防波堤结构对斜向波的反射率。数值计算结果与试验结果进行了比较，符合较好。并进一步讨论了几个重要因素对反射系数的影响。  相似文献   

Flow fields in reflected waves at a sloped sea wall     

Gerald Müller   《Ocean Engineering》2007,34(11-12):1786-1789

Wave run-up, and flow visualization experiments were conducted with a 1:2 sloped sea wall model. The visualization experiments gave an overview of flow fields in reflected, non-breaking conditions. Maximum particle velocities were found to be significantly smaller than suggested in the literature. Downrush produced a fast sheet flow, extending down to the toe of the sea wall. This created a ‘reverse’ breaker during the retreat of the initially non-breaking wave, which explains the high-energy dissipation rates for non-breaking waves reported in the literature. Embankments may therefore be exposed to wave impact pressures in areas up to 1.18H₀ below MWL.  相似文献   

Stability of waterfront retaining wall subjected to pseudo-static earthquake forces     

Deepankar Choudhury  Syed Mohd. Ahmad 《Ocean Engineering》2007,34(14-15):1947-1954

Waterfront retaining walls supporting dry backfill are subjected to hydrostatic pressure on upstream face and earth pressure on the downstream face. Under seismic conditions, if such a wall retains a submerged backfill, additional hydrodynamic pressures are generated. This paper pertains to a study in which the effect of earthquakes along with the hydrodynamic pressure including inertial forces on such a retaining wall is observed. The hydrodynamic pressure is calculated using Westergaard's approach, while the earth pressure is calculated using Mononobe-Okabe's pseudo-static analysis. It is observed that when the horizontal seismic acceleration coefficient is increased from 0 to 0.2, there is a 57% decrease in the factor of safety of the retaining wall in sliding mode. For investigating the effect of different parameters, a parametric study is also done. It is observed that if φ is increased from 30° to 35°, there is an increase in the factor of safety in the sliding mode by 20.4%. Similar observations were made for other parameters as well. Comparison of results obtained from the present approach with [Ebeling, R.M., Morrison Jr, E.E., 1992. The seismic design of waterfront retaining structures. US Army Technical Report ITL-92-11. Washington DC] reveal that the factor of safety for static condition (k_h=0), calculated by both the approaches, is 1.60 while for an earthquake with k_h=0.2, they differ by 22.5% due to the consideration of wall inertia in the present study.  相似文献   

半封闭海湾潮间带部分围垦后纳潮量计算的商榷——以胶州湾为例   总被引：14，自引：1，他引：14  

杨世伦  陈启明  朱骏  张经 《海洋科学》2003,27(8):43-47

传统的海湾(泻湖)纳潮量计算方法不能精确地反映潮间带被部分围垦后纳潮量的实际情况,本考虑围堤的影响,将纳潮量分解为堤基以下的“梯形”区和堤基以上的“矩形”区两部分之和,在理论上更加严密。根据改进后方法算出的胶州湾近期纳潮量比传统方法算出的纳潮量大15％左右。可见,半个多世纪以来胶州湾的纳潮量只减少了约15％．而不是传统方法得出的约25％。  相似文献   

波浪作用下海床动力反应的数值分析   总被引：2，自引：3，他引：2  

栾茂田  王栋 《海洋工程》2001,19(4):40-45

近海和离岸建筑物的基通常处于连续不断的小风浪作用之下，可将其变形视为弹性。基于二维广义Biot理论，提出了线性或非线性波浪作用下饱和弹性海床动力应应的时域有限元数值解法。静力平衡条件和Biot方程组成的边值方程可视为其特例，在比较算例中，数值计算得到的孔压和有效应力幅值沿海床深度的分布与解析解十分吻合。土骨架和孔隙流体的加速度对海床动力反应的影响很小。具体算例表明，线性波沿缓坡海床传播时，土层中超静孔压和有效应力幅值随之增大，有可能发生滑动坡坏。所提出的数值解法能够灵活地处理非线性波浪荷载，海底复杂地质条件和波浪沿缓坡传播等复杂情况。  相似文献   

Coastal geomorphic evolution at the Denglou Cape, the Leizhou Peninsula     

Wang Lirong  Zhao Huanting  Song Chaojing  Yuan Jiayi  Yu Hongbing 《海洋学报(英文版)》2002,21(4):597-611

ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅＤｅｎｇｌｏｕＣａｐｅ ,ｉｎｔｈｅｓｏｕｔｈｗｅｓｔｏｆｔｈｅＬｅｉｚｈｏｕＰｅｎｉｎｓｕｌａ ,ｉｓａｔｔｈｅｎｏｒｔｈｍａｒｇｉｎｏｆｔｒｏｐｉｃａｌｚｏｎｅ .ＳｅｖｅｒａｌｒｅｓｅａｒｃｈｅｓａｎｄｃａｒｔｏｇｒａｐｈｉｅｓｏｆｔｈｅｒｅｇｉｏｎａｌｇｅｏｍｏｒｐｈｏｌｏｇｙａｎｄＱｕａｔｅｒ ｎａｒｙｇｅｏｌｏｇｙ ,ｗｈｉｃｈｗｅｒｅｃａｒｒｉｅｄｏｕｔｉｎｔｈｅｐａｓｔ,ａｌｌｉｎｃｌｕｄｅｔｈｉｓａｒｅａ (ＭＧＬ ,ＳＣＳＩＯ ,ＣＡＳ ,1 978;ＧＰＣＳＧＲＣＺＴ…  相似文献   

烟台套子湾八角深水港的开发研究     

戚立心  王全东 《海岸工程》1996,15(4):9-19

通过调查和比较，认为在我国渤海和北黄海南岸的套子湾八角海区是一个条件优越的深水港址，在阐述开发八角深水港的重要意义同时，根据城市布局规划和岸线及水域资源，参照国际港口未来发展趋势，结合我国对中、西部地区经济发展战略，对八角深水港的开发和布局进行了研究，为烟台市发展成为“现代化、国际型港口城市”，提出了港区未来建设的模式。  相似文献   

海洋植物营养素的应用研究　Ⅰ.金藻类的培养     

龙丽娟  孙恢礼  何伟宏 《热带海洋学报》1998,(1)

用不同含量的93－B型海洋植物营养素对几种常用的金藻类饵料种进行培养对比试验。结果表明,海洋植物营养素用于培养金藻类效果良好,最适使用含量为30mp·L~-1域40mg·L~-1。此时效果与通常被推荐的培养液所培养的对照组相当或更好．含量过高,则造成某些营养盐过量而产生一定的毒害作用。此营养素使用简便。  相似文献