New Reference Materials,Analytical Procedures and Data Reduction Strategies for Sr Isotope Measurements in Geological Materials by LA-MC-ICP-MS     

Jacob Mulder  Graham Hagen-Peter  Teresa Ubide  Rasmus Andreasen  Ellen Kooijman  Melanie Kielman-Schmitt  Yue-Xing Feng  Bence Paul  Andreas Karlsson  Christian Tegner  Charles Lesher  Fidel Costa 《Geostandards and Geoanalytical Research》2023,47(2):311-336

Laser ablation multi-collector mass spectrometry (LA-MC-ICP-MS) has emerged as the technique of choice for in situ measurements of Sr isotopes in geological minerals. However, the method poses analytical challenges and there is no widely adopted standardised approach to collecting these data or correcting the numerous potential isobaric inferences. Here, we outline practical analytical procedures and data reduction strategies to help establish a consistent framework for collecting and correcting Sr isotope measurements in geological materials by LA-MC-ICP-MS. We characterise a new set of plagioclase reference materials, which are available for distribution to the community, and present a new data reduction scheme for the Iolite software package to correct isobaric interferences for different materials and analytical conditions. Our tests show that a combination of Kr-baseline subtraction, Rb-peak-stripping using βRb derived from a bracketing glass reference material, and a CaCa or CaAr correction for plagioclase and CaCa or CaAr + REE²⁺ correction for rock glasses, yields the most accurate and precise ⁸⁷Sr/⁸⁶Sr measurements for these materials. Using the analytical and correction procedures outlined herein, spot analyses using a beam diameter of 100 μm or rastering with a 50–65 μm diameter beam can readily achieve < 100 ppm 2SE repeatability ("internal") precision for ⁸⁷Sr/⁸⁶Sr measurements for materials with < 1000 μg g^-1 Sr.  相似文献   

SsC1qDC基因在毛蚶免疫防御中的作用          下载免费PDF全文

彭强  王进京  王春德  卢霞  刘相全  刘桂龙  徐鑫  许贺  宁军号 《海洋科学》2023,47(9):40-52

含C1q结构域蛋白(C1qDCs)在无脊椎动物免疫应答中发挥重要作用,但目前有关毛蚶(Scapharca subcrenata)C1qDCs免疫功能的研究鲜有报道。本研究从毛蚶中鉴定出一个含有典型C1q结构域的基因(命名为SsC1qDC),并分析了其对主要病原感染的响应特征。SsC1qDC的cDNA全长序列为711bp,编码含有N端信号肽和199个氨基酸的蛋白,系统进化分析显示SsC1qDC与双壳贝类的C1qDCs聚在一起。SsC1qDC在毛蚶卵细胞到眼点幼虫时期的表达量随发育阶段显著增加,且该基因在成体毛蚶各组织中均有表达,其中在肝胰腺和闭壳肌中表达水平较高。副溶血弧菌刺激可显著诱导SsC1qDC基因高表达,敲降SsC1qDC基因表达后毛蚶死亡率和血细胞凋亡水平显著升高,且导致毛蚶血细胞总数和吞噬活力显著下降。同时,敲降SsC1qDC基因的表达可显著抑制母源免疫相关基因Vg、LSZ、Dscam、TEP和SsC1qDC-3的表达,而C3、PAF3、SsC1qDC-1和SsC1qDC-2等基因的表达显著上调。本研究结果表明, SsC1qDC在毛蚶抗弧菌响应中发挥重要作用,对贝类免疫学和抗...  相似文献