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101.
Hydrocarbon degraders from tropical marine environments 总被引:1,自引:0,他引:1
Analysis of 20 samples of marine mud and water around Mumbai resulted in the isolation of 17 bacteria and yeasts all of which were able to degrade more than 10% of the supplied crude oil. The yeasts strains were important degraders of the aliphatic fraction of crude. All the isolated yeasts belonged to the genus Candida. Using biochemical tests these were identified as Candida parapsilosis, C. albicans, C. guilliermondii, Yarrowia lipolytica, C. tropicalis and C. intermedia. Y. lipolytica was the best degrader utilizing 78% of the aliphatic fraction of Bombay High crude oil. None of these isolates degraded the aromatic or ashphaltene fractions. All the isolates required aeration, nitrogen and phosphate supplementation for optimal degradation. Four out of the six yeasts are human pathogens. 相似文献
102.
Relationship between structures of substituted indolic compounds and their degradation by marine anaerobic microorganisms 总被引:1,自引:0,他引:1
Degradation of selected indolic compounds including indole, 1-methylindole, 2-methylindole, and 3-methylindole was assessed under methanogenic and sulfate-reducing conditions using the serum-bottle anaerobic technique and marine sediment from Victoria Harbour, Hong Kong as an inoculum. Our results showed that indole degradation was achieved in 28 days by a methanogenic consortium and 35 days by a sulfate-reducing consortium. During degradation under both conditions, two intermediates were isolated, purified and identified as oxindole and isatin (indole-2,3-dione) suggesting that both methanogenic and sulfate-reducing bacteria use an identical degradation pathway. Degradation processes followed two steps of oxidation accomplished by hydroxylation and then dehydrogenation at 2- and then 3-position sequentially prior to the cleavage of the pyrrole ring between 2- and 3-positions. However, none of 1-methylindole or 2-methylindole was degraded under any conditions. 3-Methylindole (3-methyl-1H-indole, skatole) was transformed under methanogenic conditions and mineralized only under sulfate-reducing conditions. It is clear that methyl substitution on 1- or 2-position inhibits the initial attack by hydroxylation enzymes making them more persistent in the environment and posing longer toxic impact. 相似文献
103.
阿拉斯加输油管的设计和施工方式方案变更过程及其背后的原因和哲学思想 总被引:3,自引:1,他引:3
阿拉斯加输油管道公司曾经研发和使用了3种主要施工方案和技术:传统地埋式、特殊埋设和地上敷设方式.在传统地埋式施工方案中,管顶埋深变化于0.9~2.7 m.这主要是考虑了地形变化,而不是沿途的岩性和土壤类型.在特殊埋设方案中,在原油管道必须埋设的地段,如大规模动物迁徙常用地段则使用通道冷液循环降(保)温系统和(或)热管(桩)降(保)温,来保护多年冻土.地上敷设方案包括桩基架设和地上洁净砾石管堤(垫护层).后者只在管道进入和离开多年冻土时采用.架设桩基横梁方案中使用有或没有热管保温的垂直支架梁(单元)(VSM).在初步规划和设计阶段,管道的业主公司(即横穿阿拉斯加管道系统,或TAPS)和随后的阿拉斯加管道服务公司(APSC)不容商量的坚决要求100%的埋设方案.但是,随着勘察工作的进展和设计方案的细化,施工设计方案在不断变化.1977年管道施工结束时,只有57%的管道采用了埋设方式.管道运行30 a后的今天(考虑运行期间维护中所产生的问题),很多经验丰富的工程师认为53%的埋设可能更合理.设计和施工方案变更的原因主要有:1)为了获得通过联邦政府所属的土地所需的许可证,政府有特殊的规定和要求;2)管道公司的设计、施工和管理人员进行了现场野外调查、研究,并积极参与了详细设计和研发;3)美国环境政策法案(NEPA)的最新要求(启用了核准制).阿拉斯加管道服务公司是7家主要石油公司的服务机构.由于这个项目的巨大规模和所涉及的高昂费用,致使各大石油公司的工程师非同寻常程度的参与.在文章中,笔者论述了与阿拉斯加管道施工有关的阿拉斯加管道服务公司、美国联邦和阿拉斯加州政府相关组织机构的形成历史,管道设计演变过程及其背后的哲学思想,以及阿拉斯加管道工程项目的经验和教训. 相似文献
104.
Permafrost on the Qinghai-Tibet Plateau (QTP) is widespread, thin, and thermally unstable. Under a warming climate during the past few decades, it has been degrading extensively with generally rising ground temperatures, the deepening of the maximum summer thaw, and with lessening of the winter frost penetration. The permafrost has degraded downward, upward and laterally. Permafrost has thinned or, in some areas, has totally disappeared. The modes of permafrost degradation have great significance in geocryology, in cold regions engineering and in cold regions environmental management. Permafrost in the interior of the QTP is well represented along the Qing-hai-Tibet Highway (QTH), which crosses the Plateau through north to south and traverses 560 km of permafrost-impacted ground. Horizontally, the degradation of permafrost occurs more visibly in the sporadic permafrost zone in the vicinity of the lower limit of permafrost (LLP), along the margins of taliks, and around permafrost islands. Downward degradation develops when the maximum depth of seasonal thaw exceeds the maximum depth of seasonal frost, and it generally results in the formation of a layered talik disconnecting the permafrost from the seasonal frost layer. The downward degrada- tion is divided into four stages: 1) initial degradation, 2) accelerated degradation, 3) layered talik and 4) finally the conversion of permafrost to seasonally frozen ground (SFG). The upward degradation occurs when the geothermal gradient in permafrost drops to less than the geothermal gradients in the underlying thawed soil layers. Three types of permafrost temperature curves (stable, degrading, and phase-changing transitory permafrost) illustrate these modes. Although strong differentiations in local conditions and permafrost types exist, the various combinations of the three degradation modes will ultimately transform permafrost into SFG. Along the QTH, the downward degradation has been proceeding at annual rates of 6 to 25 cm, upward degradation at 12 to 30 cm, and lateral degradation in the sporadic permafrost zone at 62 to 94 cm during the last quarter century. These rates exceed the 4 cm per year for the past 20 years reported for the discontinuous permafrost zone in subarctic Alaska, the 3 to 7 cm per year reported in Mongolia, and that of the thaw-stable permafrost in subarctic Yakutia and Arctic Alaska. 相似文献
105.
祁连山多年冻土区天然气水合物的形成条件 总被引:9,自引:0,他引:9
祁连山多年冻土区面积约10×104km2,年平均地表地温为-1.5~-2.4℃,冻土层厚度为50~139m.区内侏罗纪小型含煤盆地广布,产有丰富的煤层气.南祁连盆地是一潜在的油气盆地,存在下石炭统臭牛沟组、下二叠统草地沟组、中三叠统大加连组、上三叠统尕勒得寺组4套烃源岩,具有良好的生油生气潜力.在木里煤田33号钻孔的冻土层内发现有连续逸出的高含量烃类气体,井口点燃即可燃烧,简易采气分析结果表明甲烷含量高达38.07%~75.9%.根据该钻孔的气体组分、年平均地表地温、冻土层厚度、地温梯度等数据分析,这里基本具备形成天然气水合物的温压条件,计算结果显示水合物稳定带的顶界和底界埋深分别为171 m和574m,稳定带厚度为403m. 相似文献
106.
107.
108.
王万春 《矿物岩石地球化学通报》2007,26(3):269-275
微生物降解使有机化合物的稳定碳、氢同位素发生不同程度分馏的研究在有机污染物来源和微生物环境修复等领域取得了长足进展,并对原油和天然气微生物降解研究有借鉴意义。微生物作用下的同位素分馏为动力同位素分馏,导致重同位素在残余物中富集。影响微生物降解有机物同位素分馏的主要因素有微生物的降解代谢途径、辅酶作用、降解类型与程度、同位素质量差异和有机物碳数等。不同的微生物代谢途径代表不同的生物化学反应,造成了同位素分馏的显著差异;辅酶对反应的催化作用使微生物作用造成的同位素分馏更加复杂。低碳数正构烷烃遭受微生物降解程度越高,碳、氢同位素的分馏也越大,同位素变重与降解程度之间有明显的相关性。但对于复杂化合物,由于降解的多级反应,同位素分馏与降解程度间的相关性并不明显。在同样降解程度下,氢同位素分馏大于碳同位素分馏,低碳数正构烷烃的同位素分馏大于高碳数正构烷烃的同位素分馏。 相似文献
109.
天然气水合物大致经历了实验室研究、管道堵塞及防治、资源调查与开发利用4个发展阶段,中国目前正处于资源调查阶段的早期。自1999年开始,中国先后开展了南海、东海、陆上冻土区和国际海底区域天然气水合物的调查研究,相继发现了一系列地质、地球物理和地球化学异常标志,并在南海北部神狐地区成功采到水合物实物样品,取得了找矿工作的重大突破,显示出良好的找矿前景,但目前仍存在着调查研究程度较低、技术装备比较落后等问题。随着国家对天然气水合物重视程度的加强,中国天然气水合物的调查研究进程将会将进一步加快,并在不久的将来过渡到试生产阶段和商业性生产阶段。 相似文献
110.
The development and utilization of water and land resources in the Shiyang River basin are the highest among the inland rivers
in northwestern China. Using GIS and the landscape structure analysis program FRAGSTATS, landscape metrics of a study area
in the lake-district in Minqin Oasis in the lower reaches of Shiyang River basin were calculated and analyzed based on TM
images from 1987 to 2001 at class level and landscape level, respectively. Transformation matrix of the landscape mosaics
was also examined. The driving forces of landscape changes were analyzed, including the dynamics of runoff and groundwater
resources and their exploitation, and influences of policies and market economy. The results showed that: (1) Patch number
of farmland and desert decreased, but their mean patch area increased, indicating that reclamation and desertification was
very serious in the study area. Contagion index for oasis increased and edge density and landscape diversity were reduced.
(2) Farmland and desert areas have enlarged by 1,520.46 and 4,941.27 hm2, respectively. This increase was at the cost of woodland and grassland, which shrank by 1,520.46 and 4,941.27 hm2, respectively. (3) The net utilization of surface water decreased by 50% in the last 10 years, and dropped to 22.3 × 106 m3 in the later 1990s. Exploitation of groundwater increased remarkably, up to 80 × 106 m3 per year in the last 5 years. (4) The capability of the economic structure to withstand pressures of the market economy has
depressed distinctly because of the simplified planting pattern, which is dominated by salinity-enduring cash crops, such
as cotton. Based on the above analysis, strategies of ecological reconstruction in the lake-district were suggested. 相似文献