Application of Atomic Force Microscopy to Observation of Marine Bacteria   总被引：3，自引：0，他引：3  

Tomohiko Nishino  Eiko Ikemoto  Kazuhiro Kogure 《Journal of Oceanography》2004,60(2):219-225

The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as a new tool to study the fine structures of marine bacteria. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Salt Diapirs, Salt Brine Seeps, Pockmarks and Surficial Sediment Creep and Slides in the Canary Channel off NW Africa     

J. Acosta  E. Uchupi  A. Muñoz  P. Herranz  C. Palomo  M. Ballesteros 《Marine Geophysical Researches》2003,24(1-2):41-57

Circular to elliptical mounds in the Canary Channel with reliefs of 75 to 375 m and diameters of 4 to 8 km partially surrounded by moats with reliefs of 25 to 75 m, were formed by piercement of the seafloor by Mesozoic evaporites. Several long gullies, < 1km wide, with abrupt terminations and pockmarks associated with these mounds were probably eroded by dense brine and hydrocarbon seeps. The salt brines that eroded the gullies were formed where salt diapirs intersect the seafloor, or in the subsurface by circulating ground water heated by igneous activity along the Canary Ridge. If the brines originated in the subsurface they reached the seafloor along faults. Displacement of the surficial sediments by sliding and creep is probably the result of the expulsion of hydrocarbons and/or vertical motion of the Mesozoic evaporites. Microtopographic features along or near the east flank of the Canary Ridge are the creation of uplift of the ridge, hydrothermal activity, mass wasting processes and turbidity currents.  相似文献   

波群作用于垂直桩柱的峰力统计性质     

徐立论  陈志军  焦海英 《中国海洋大学学报(自然科学版)》1994,(1)

研究具有窄谱和Ｗｅｉｂｕｌｌ波高分布的波群对非线性桩柱系统作用力的统计性质。求得了桩桩的波浪峰力的各种特征值及其比值。指出这些数值不仅是阻力一惯性力参数ｂＨ的函数，也随着波群因子而变化。本文模式更具广泛性。文中给出了一系列计算图表，可从理论计算波群作用于桩柱的波浪峰力。  相似文献   

A study of Coriolis effects on long waves propagating in an unbounded ocean, channel and basin     

Ashabul Hoque  Azizur Rahman 《Ocean Engineering》2007,34(11-12):1701-1705

The effects of Coriolis force on long waves have been discussed based on gravity waves propagating in an unbounded ocean, channel and basin. In case of ocean, results show that the Coriolis effect will be significant and negligible, when the wave period is comparable to 2π/f and much shorter, respectively. Results also show in a channel, the wave amplitude and water particle velocity decrease exponentially in the positive y direction in the northern hemisphere (where f is positive). Moreover, in a basin, the Cotidal lines have been found as curves and rotate counterclockwise around the origin.  相似文献   

波浪底摩擦力对重砂富集的影响——以鲁南近岸区为例     

金秉福 《海洋科学》1997,21(5):49-52

根据浅水波浪的底摩擦效应，对鲁南灵山湾和臼－岚山镇近岸区进行了波浪的海底摩擦力计算。波浪的海底摩擦力等值图与已知滨海锆石砂矿资料对比表明，底摩擦力大于０．１８Ｎ／ｍ＾２的动力分选区有利于重矿物砂富集。据此，进一步预测了鲁南近岸带的砂矿富集区。  相似文献   

McCreary和Fickian湍扩散形式差异的分析     

吴德星  李国璋  李宝辉  陈峰 《中国海洋大学学报(自然科学版)》1993,(Z2)

用矩阵方法,求解McCreary和Fickian铅垂向湍扩散形式下,无界海洋条件时在风应力和深层浮力作用下的线性解。得出不同的湍扩散系数A情况下McCreary与Fickian湍扩散形式的流场结构。指出随湍扩散系数的增加,McCreary和Fickian湍扩散形式下u分量结构及其量值差异增大。在Fickian湍扩散形式下,能量穿越密度跃层的传递能力较强。当A=0.1cm~2s~(-3)时,在风应力和深层浮力作用下两种湍扩散形式造成流场的差异很小。提出在小湍扩散系数下,用McCreary湍扩散形式研究热带海洋环流,其结果和Fickian形式应相当一致。  相似文献   

Non-Simultaneous Failure of Ice in Front of Multi-Leg Structures   总被引：4，自引：1，他引：4  

SHI  Qingzeng 《中国海洋工程》2002,16(2):183-192

Because the multi-leg jacket structure is the major type of offshore structures in the Bohai Sea, the study of non-si-multaneous failure of i鏴 on multi-leg structures is important. However, the non-simultaneous failure has not been consid-ered in engineering design until now, obviously resulting in costly design and notable waste. To resolve this problem, this paper, by means of analysis of experimental data, calculates the coefficient of the non-simultaneous failure for the double-pile structure, the square four-leg structure, the single-line multi-pile structure, and the conical structure, respectively, and provides some reference criteria for engineering design.  相似文献   

科氏力对河口分汊的影响   总被引：1，自引：0，他引：1       下载免费PDF全文

金元欢  沈焕庭 《海洋科学》1993,17(4):52-56

以长江口、黄河口、珠江口、闽江口等几个分汊河口为例,分析了科氏力在河口分汊中的作用。结果表明,涨落潮流路分歧主要通过形成涨落潮冲刷槽和中央缓流区浅滩而逐渐使河口产生分汊;水面横比降则以横向环流和横向切滩两种形式,或堆积或冲刷,导致河口分汊。  相似文献   

Fluid forces on a small cylinder in the presence of a large cylinder in relative oscillatory flow     

C.H.K. Williamson 《Applied Ocean Research》1985,7(3):124-127

The present brief paper is intended to show that the fluid forces on a small cylinder can be considerably magnified when it is in the flow field of a larger cylinder. Two cylinders of unequal diameter are oscillated in a tank of fluid, and the lift and in-line forces on the smaller cylinder are measured when the pair of cylinders is placed at various orientations and spacings.  相似文献   

Bottom Simulating Reflector and Gas Seepage in Okinawa Trough： Evidence of Gas Hydrate in an Active Back-Arc Basin     

栾锡武  Kelin  Wang  Roy  Hyndman  Eleanor  Willoughby 《中国地质大学学报(英文版)》2008,19(2):152-161

To look for gas hydrate, 22 multi-channel and 3 single-channel seismic lines on the East China Sea (ECS) shelf slope and at the bottom of the Okinawa Trough were examined. It was found that there was indeed bottom simulating reflector (BSR) occurrence, but it is very rare. Besides several BSRs, a gas seepage was also found. As shown by the data, both the BSR and gas seepage are all related with local geological structures, such as mud diapir, anticline, and fault-controlled graben-like structure. However, similar structural "anomalies" are quite common in the tectonically very active Okinawa Trough region, but very few of them have developed BSR or gas seepage. The article points out that the main reason is probably the low concentration of organic carbon of the sediment in this area. It was speculated that the rare occurrence of gas hydrates in this region is governed by structure-controlled fluid flow. Numerous faults and fractures form a network of high-permeability channels in the sediment and highly fractured igneous basement to allow fluid circulation and ventilation. Fluid flow in this tectonic environment is driven primarily by thermal buoyancy and takes place on a wide range of spatial scales. The fluid flow may play two roles to facilitate hydrate formation:to help gather enough methane into a small area and to modulate the thermal regime.  相似文献