Extraction of soluble collagen and its feasibility in the palaeodietary research     

WANG Ning  HU YaoWu  HOU LiangLiang  YANG RuiPing  SONG GuoDing  WANG ChangSui 《中国科学:地球科学(英文版)》2014,57(5):1039-1047

In current palaeodietary research, gelatinization is the main method to extract insoluble collagen(ISC) from ancient bones. However, the degradation products of ISC, i.e., soluble collagen(SC), is often neglected and abandoned. In this work, we try to separate the extracts of ancient bones using gel chromatography and compare the contents of carbon and nitrogen, atomic C/N ratio, and stable carbon and nitrogen isotopic values of the extracts from three peaks to determine which peak can be attributed to SC. At last, the potential application of SC in palaeodietary research is discussed based on the comparison of stable isotopic values between ISC and SC. Among the three peaks, the second with the retention time between 17.5 min and 27.5 min had the most broad peak shape, indicating that the molecular weights of proteins collected were most variable. Besides, the contents of carbon and nitrogen and atomic C/N ratio of extracts in this peak were closest to the corresponding ISC. Based on the above, we conclude that the extract in second peak is SC. More important, the δ 13C and δ 15N values of ISC and SC are very similar. For ISC and SC with atomic C/N ratios within the normal range(2.9–3.6), the mean difference of δ 13C value was only(0.3±0.2)‰(n=2) while δ 15N value was(0.6±0.1)‰(n=2). Although the atomic C/N ratios of some SC are slightly beyond the normal range, the mean differences of δ 13C and δ 15N values were still only(0.4±0.1)‰ and(0.3±0)‰(n=2) respectively. These isotopic differences are quite below the isotope fractionation in one trophic level(δ 13C values of 1‰–1.5‰ and δ 15N values of 3‰–5‰), suggesting that SC had great application potentials in palaeodietary research.  相似文献   

Ion dynamics associated with substorm dipolarization fronts     

Song Fu  Maha Ashour-Abdalla  XiaoHua Deng  Mostafa El-Alaoui  Meng Zhou  Robert L. Richard  Raymond J. Walker  ZhiGang Yuan 《中国科学:地球科学(英文版)》2014,57(10):2543-2551

We report where and how ions are accelerated in the proximity of earthward propagating dipolarization fronts(DFs) in the magnetotail during a magnetospheric substorm on February 15, 2008. Two DFs were observed by multiple THEMIS spacecraft in the near-Earth magnetotail(~?10 Re). We studied the ion dynamics associated with these DFs by comparing observed results with large scale kinetic(LSK) simulation results. The LSK simulation reproduced the sudden ion energy flux enhancement concurrent with the arrival of the DF at the satellite locations. We found that ions can be accelerated to more than 100 keV energy at the DF. These ions were initially non-adiabatically accelerated near magnetic reconnection site and then still non-adiabatically accelerated at the DF structure.  相似文献   

NaCl胁迫下红砂愈伤组织中主要离子累积特征的研究   总被引：4，自引：2，他引：2  

谭会娟  贾荣亮  刘玉冰  赵昕  李新荣 《中国沙漠》2010,30(6):1305-1310

盐胁迫下植物对Na+、K+、Ca2+、Cl-的选择性吸收代表了植物对盐胁迫的适应性。以荒漠植物红砂为材料,研究不同浓度NaCl胁迫对红砂愈伤组织Na+、K+、Ca2+、Cl-含量的影响。结果表明,NaCl胁迫下红砂组织相对生长率随着盐浓度的增加先增加后下降, 100 mM处理时达到最大;K+、Ca2+含量下降,Na+和Cl-含量升高,变化幅度随NaCl浓度的增加而增大。红砂愈伤组织具有很强的耐盐能力,低浓度NaCl处理可促进愈伤组织的生长,对Na+、K+、Ca2+、Cl-的吸收选择性提高,有利于避免离子代谢紊乱,减轻NaCl胁迫的伤害;在较高浓度下盐胁迫会对植物的生长造成一定的抑制。可见,离子平衡是红砂组织耐盐性的重要机理之一。  相似文献   

Zn(II) Ion Biosorption onto Surface of Eucalyptus Leaf Biomass: Isotherm,Kinetic, and Mechanistic Modeling     

Vishal Mishra  Chandrajit Balomajumder  Vijay Kumar Agarwal 《洁净——土壤、空气、水》2010,38(11):1062-1073

Bioremediation of Zn(II) by biosorption across aqueous phase on to surface of eucalyptus leaf powder has been investigated in present research work. The adsorptive potential of eucalyptus leaf powder was evaluated as function of pH, temperature, contact time, agitation rate and particle size. Maximum metal ion uptake and percentage removal capacity of eucalyptus leaf powder were 23.5 mg g⁻¹ and 94%, respectively, at optimized pH 5, 20 ± 1°C, contact time 6 h, particle size 0.5 mm and agitation rate 200 rpm. The biomass surface analysis revealed the fact that the biomass surface was heterogeneous and porous in nature. The functional groups like amine, amide, carboxyl, hydroxyl, and methyl groups, significantly important for metal ion binding were present on biomass surface in tremendous amount. Additionally, the Fourier transformation IR spectrum analysis of acid and base activated eucalyptus leaf biomass ruled out all the possibilities of the presence of surface functional groups mentioned above. The reaction rate was studied by applying two rate limiting models pseudo first and pseudo second order. Pseudo second order model was found to be more suitable (R² = 0.998) in comparison to pseudo first order (R² = 0.724). Adsorption equilibrium of batch stirred reaction data fitting shows the dominance of Langmuir isotherm (R² = 0.99) against Freundlich isotherm (R² = 0.887) model with equipartitional involvement of both film and intra particle diffusion as rate limiting steps at differential status of contact time.  相似文献   

福建地下流体氟离子动态非地震前兆异常识别     

林国元 《地震地磁观测与研究》2010,31(4):57-60

2008年3—7月福建地区共发生4次中等地震,福建地下流体氟离子有多孔观测井,在震前或震后出现高值突跳的现象;2008年9-11月福建地下流体氟离子又有多孔观测井出现高值异常。本研究通过实验提出:该氟离子高值异常为非地震前兆异常,建议准备一支氟离子选择性电极作为备用电极,用于对比观测,以提高氟离子观测数据异常的判别。  相似文献   

孔隙水中NH_4~+和HPO_4~(2-)浓度异常:一种潜在的天然气水合物地球化学勘查新指标   总被引：6，自引：3，他引：3  

杨涛  蒋少涌  杨竞红  葛璐  凌洪飞  吴能友  陈道华 《现代地质》2005,19(1):55-60

在海洋天然气水合物的勘查与研究中地球化学方法发挥了重大作用。利用海洋沉积物孔隙水中各种离子含量和同位素异常可以有效示踪天然气水合物的存在, 其中Cl-浓度异常、SO42-浓度梯度和氧同位素组成异常已成为指示天然气水合物存在的灵敏示踪剂。通过对比有和没有天然气水合物存在的海域浅表层沉积物中孔隙水的NH4+和HPO42-含量, 发现有天然气水合物区的孔隙水中NH4+和HPO42-浓度明显偏高, 且存在与SO42-类似的浓度梯度曲线, 认为孔隙水中NH4+和HPO42-浓度异常有可能成为一种新的地球化学示踪剂, 指示天然气水合物的存在; 探讨了引起孔隙水中NH +和HPO 2-浓度异常的可能机理及精确测定这些离子含量的方法。  相似文献   

The role of vibrationally excited nitrogen and oxygen in the ionosphere over Millstone Hill during 16-23 March, 1990     

A. V. Pavlov  K.-I. Oyama 《Annales Geophysicae》2000,18(8):957-966

We present a comparison of the observed behavior of the F region ionosphere over Millstone Hill during the geomagnetically quiet and storm period on 16/23 March, 1990, with numerical model calculations from the time-dependent mathematical model of the Earths ionosphere and plasmasphere. The effects of vibrationally excited N₂(v) and O₂(v) on the electron density and temperature are studied using the N₂(v) and O₂(v) Boltzmann and non-Boltzmann distribution assumptions. The deviations from the Boltzmann distribution for the first five vibrational levels of N₂(v) and O₂(v) were calculated. The present study suggests that these deviations are not significant at vibrational levels v = 1 and 2, and the calculated distributions of N₂(v) and O₂(v) are highly non-Boltzmann at vibrational levels v > 2. The N₂(v) and O₂(v) non-Boltzmann distribution assumption leads to the decrease of the calculated daytime NmF2 up to a factor of 1.44 (maximum value) in comparison with the N₂(v) and O₂(v) Boltzmann distribution assumption. The resulting effects of N₂(v > 0) and O₂(v) > 0) on the NmF2 is the decrease of the calculated daytime NmF2 up to a factor of 2.8 (maximum value) for Boltzmann populations of N₂(v) and O₂(v) and up to a factor of 3.5 (maximum value) for non-Boltzmann populations of N₂(v) and O₂(v). This decrease in electron density results in the increase of the calculated daytime electron temperature up to about 1040/1410 K (maximum value) at the F2 peak altitude giving closer agreement between the measured and modeled electron temperatures. Both the daytime and nighttime densities are not reproduced by the model without N₂(v > 0) and O₂(v > 0), and inclusion of vibrationally excited N2 and O2 brings the model and data into better agreement. The effects of vibrationally excited O2 and N2 on the electron density and temperature are most pronounced during daytime.  相似文献   

A self-consistent estimate of O+ + N2– rate coefficient and total EUV solar flux with λ < 1050 Å using EISCAT observations     

A. V. Mikhailov  K. Schlegel 《Annales Geophysicae》2000,18(9):1164-1171

There are differences between existing models of solar EUV with < 1050 Å and between laboratory measurements of the O⁺ + N₂ – reaction rate coefficient, both parameters being crucial for the F2-region modeling. Therefore, indirect aeronomic estimates of these parameters may be useful for qualifying the existing EUV models and the laboratory measured O⁺ + N₂ – rate coefficient. A modified self-consistent method for daytime F2-region modeling developed by Mikhailov and Schlegel was applied to EISCAT observations (32 quiet summer and equinoctial days) to estimate the set of main aeronomic parameters. Three laboratory measured temperature dependencies for the O⁺ + N₂ – rate coefficient were used in our calculations to find self-consistent factors both for this rate coefficient and for the solar EUV flux model from Nusinov. Independent of the rate coefficient used, the calculated values group around the temperature dependence recently measured by Hierl et al. in the 850–1400 K temperature range. Therefore, this rate coefficient may be considered as the most preferable and is recommended for aeronomic calculations. The calculated EUV flux shows a somewhat steeper dependence on solar activity than both, the Nusinov and the EUVAC models predict. In practice both EUV models may be recommended for the F2-region electron density calculations with the total EUV flux shifted by ±25% for the EUVAC and Nusinov models, correspondingly.  相似文献   

黄土-古土壤沉积物中酸溶相硼的分离及其同位素测定   总被引：5，自引：0，他引：5  

赵志琦  刘丛强  肖应凯 《地球化学》2000,29(4):351-357

用０．５ｍｏｌ／ＬＨＣｌ提取黄土、古土壤中的酸溶相成分,并用混合树脂法和硼特效树脂法两方法叠加以硼进行分离和纯化,Ｃｓ２ＢＯ２＾＋法测同位素比值。结果表明,虽然混合树脂法和硼特效脂法都是较成熟的方法,但用来处理杂质离子含量相对高而硼含量低的样品深液并不成功。两种方法叠加处理后达到理想的效果,重复处理黄土－古土壤样品的精度（２ＲＳＤ）在０．４‰～０．４‰之间,这与标样（ＮＩＳＴ ＳＲＭ９５１）水平（  相似文献   

离子交换反应合成铅长石的实验研究     

刘瑞  鲁安怀秦善 《岩石矿物学杂志》2005,24(6):511-514

对长石与Pb(NO_3)_2粉末在380℃条件下反应48h的产物进行XRD分析,显示出现了d值分别为6.54、3.42、3.32、3.27,2.57的铅长石的5个特征峰。XPS分析表明Pb4f7/2在长石矿物中的结合能为138.39~138.44eV。实验证明Pb2 与长石结构中的碱性或碱土性离子发生了离子交换反应,生成了铅长石。  相似文献