运用3DEEMs及荧光偏振方法研究pH、离子强度及浓度效应对腐殖酸荧光光谱特性的影响   总被引：6，自引：0，他引：6  

梅毅  吴丰昌  王立英  白英臣 《地球化学》2008,37(2):165-173

利用三维荧光激发.发射光谱(3DEEMs)及荧光偏振法研究了3例腐殖酸和1例湖泊溶解有机物(DOM)在不同腐殖酸浓度、离子强度和pH条件下的荧光光谱特性.实验结果表明,随着浓度增大,各腐殖酸样的荧光强度增大,其中垃圾渗滤液腐殖酸和Pahokee泥炭腐殖酸的荧光峰出现红移.随着浓度进一步增大,腐殖酸的荧光偏振值增大,由此推断在此浓度范围内腐殖酸由于分子间的排斥作用使其构型出现伸展.离子强度增大导致腐殖酸及阿哈湖DOM的荧光强度降低.随着氯化钾(KCI)浓度增大,Fluka腐殖酸的荧光峰A蓝移,荧光峰B的荧光偏振值出现减小,推测是由于静电中和作用使其荧光基团发生卷曲造成的.离子强度对其余腐殖酸样和阿哈湖DOM的其他荧光特性没有明显的影响.总的来说,pH值的变化对腐殖酸和湖泊DOM的荧光特征的影响是很明显的.随着pH值增大,腐殖酸和湖泊DOM的荧光强度增强.而pH值减小时,Fluka腐殖酸和Pahokee泥炭腐殖酸的荧光峰(A峰)蓝移,当pH值降到5时,Fhka腐殖酸的荧光峰突然红移到最大,随后又继续蓝移;垃圾渗滤液腐殖酸和阿哈湖DOM的荧光峰位无明显的漂移.所有样品的荧光偏振值都减小(但阿哈湖DOM的A峰变化不大),但在pHi≤5时又增大,这种现象表明随着pH值降低,腐殖酸和湖泊DOM中有机大分子发生卷曲,但在pH≤5时由于羧基的质子化又以平面伸展构型出现在溶液中.  相似文献   

Optical spectrum based measurement of flotation slurry contents   总被引：1，自引：1，他引：0  

Olli Haavisto  Jani Kaartinen  Heikki Hytyniemi 《International Journal of Mineral Processing》2008,88(3-4):80-88

Monitoring and control of a flotation circuit is mainly based on the information gained by assaying the process slurry lines. In flotation plants, usually an X-ray fluorescence analyzer is used to obtain the on-line assays. This article introduces the visual and near-infrared reflectance spectroscopic analysis of the process slurries as a supplementary method which complements the on-line assay information available from an X-ray fluorescence analyzer. It is shown that the spectral information can be used to accurately predict the element contents in the slurry in between successive XRF analyses. Since the spectral measurements can be taken with high frequency as opposed to the sparse X-ray fluorescence analysis, a practically continuous on-line estimate of the slurry contents is reached. These estimates can be used in the plant control to improve the overall performance of the circuits and to yield also economical savings. Additionally, the frequent monitoring of the grades can provide means to eliminate rapid disturbances in the circuits, thus improving the stability of the process.  相似文献   

Egg production of Calanus finmarchicus—A basin-scale study     

Erling K. Stenevik  Webjrn Melle  Eilif Gaard  Astthor Gislason  Cecilie T.. Broms  Irina Prokopchuk  Bjrnar Ellertsen 《Deep Sea Research Part II: Topical Studies in Oceanography》2007,54(23-26):2672

Egg production of Calanus finmarchicus was studied during joint basin-scale surveys in April–June 2003 in the Norwegian Sea. Surveys covered the whole Norwegian Sea and were conducted from Norwegian, Icelandic and Faroese research vessels. Stations were classified as being in pre-bloom, bloom or post-bloom phase according to levels of chlorophyll a and nitrate. Individual egg production rates and population egg production rates were calculated and compared between areas. Both individual egg production rates (eggs female⁻¹ day⁻¹) and population egg production rates (eggs m⁻² day⁻¹) were significantly higher in bloom areas compared with pre-bloom and post-bloom areas. However, when integrated over an estimated duration of the three phases, the time-integrated egg production (eggs m⁻²) in most years was highest in the pre-bloom phase, and this was explained by the longer duration of this phase compared with the two other phases.  相似文献   

基于荧光原理的叶绿素a传感器校准方法研究     

《海洋技术学报》2024,(1)

利用传感器对水体叶绿素a 浓度进行原位测量是获取实时、连续、长时间序列数据的重要手段。本文在对RBR 传感器和ECO（Environmental Characterization Optics）传感器进行原理分析和线性度、稳定性、重复性等基本性能测试的基础上，利用单一藻种培养液和2020年南海北部海域现场数据校准传感器，并对新的传感器校准系数进行验证。结果表明：两台传感器使用新系数比原出厂系数的叶绿素a 浓度测量准确度有明显提高。RBR 传感器现场数据校准系数的计算结果与叶绿素a 标准值误差最小，平均绝对误差从1.93 μg/L 减小到0.35 μg/L，平均相对误差从55.1%减小到10.9%；ECO 传感器藻液系数明显优于出厂系数和现场数据校准系数的计算结果，平均绝对误差从1.76 μg/L 减小到0.59 μg/L，平均相对误差从50.3%减小到15.1%。传感器测量准确度的提高，可为海洋环境监测、海洋生态灾害预警等工作获取真实可靠数据提供支撑。  相似文献   

Nutrient dynamics and seasonal succession of phytoplankton assemblages in a Southern European Estuary: Ria de Aveiro, Portugal   总被引：2，自引：1，他引：2  

C.B. Lopes  A.I. Lilleb  J.M. Dias  E. Pereira  C. Vale  A.C. Duarte 《Estuarine, Coastal and Shelf Science》2007,71(3-4):480-490

The spatial and temporal dynamics of dissolved inorganic nitrogen, dissolved phosphate, dissolved silica and chlorophyll a were measured seasonally at eight stations in the Ria de Aveiro. Between December 2000 and September 2001, the seasonal succession of phytoplankton assemblages, inferred after the spatial and seasonal variation of silica and of chlorophyll a concentrations, showed that diatoms (μmol Si L⁻¹) dominated from late autumn until early spring, while chlorophytes (μg Chl a L⁻¹) bloomed during late spring and summer. The Si:N:P ratios and Si concentrations indicated no seasonal depletion in dissolved silica, as in other temperate systems, possibly because of abnormal precipitation and flood events prolonging the supply of dissolved Si to the system. The Si:N:P ratios suggested P limitation at the system level. Despite the relative proportions of available nutrients, the mean phosphorus concentration (5.3 μmol L⁻¹) was above the reported half-saturation constants for P uptake by phytoplankton. Thus, in Ria de Aveiro, the seasonal succession of phytoplankton assemblages may also be dependent on the grazing capacity of the pelagic community through top-down regulation.  相似文献   

Microphytobenthos vertical migratory photoresponse as characterised by light-response curves of surface biomass     

Joo Serdio  Helena Coelho  Snia Vieira  Snia Cruz 《Estuarine, Coastal and Shelf Science》2006,68(3-4):547

The migratory response of intertidal microphytobenthos to changes in irradiance was studied on undisturbed estuarine sediments. Two non-destructive optical techniques were used to trace variations in vivo of surface biomass: PAM fluorometry, for measuring the minimum fluorescence level (F_o); and spectral reflectance analysis, for quantifying the normalized difference vegetation index (NDVI). Following the formation of a dense biofilm at the surface, replicated sediment samples were simultaneously exposed to six different irradiance levels, ranging from 50 to 1500 μmol m⁻² s⁻¹, during a period of 120 min. The migratory photoresponse of the biofilms was characterised by constructing biomass vs. light curves (BLC), relating the accumulation of microalgal biomass after that period (estimated by F_o or NDVI) to the irradiance level incident on the surface. BLCs allow characterising the main features of the migratory photoresponse of intact biofilms. Typical BLC showed a clear biphasic pattern, with an increase in microalgal accumulation under irradiances below 100 μmol m⁻² s⁻¹, maximum values under 100–250 μmol m⁻² s⁻¹, and a gradual decrease of surface biomass under higher irradiances, indicating a strong photophobic downward migratory response. Similar BLC patterns were obtained when measuring F_o or NDVI. The construction of BLCs for biofilms from intertidal sites with distinctive sediment characteristics and diatom taxonomic composition allowed to detected significant differences in the migratory photoresponse. Biofilms from a muddy sediment exhibited considerably larger amplitude in the migratory photoresponse than the biofilms from a sandy mud site, especially under high irradiances. The photophobic migratory response to high light was found to vary among diatom species, particularly in the case of the biofilms from the muddy sediments.  相似文献   

厦门湾有色溶解有机物的光吸收特性研究   总被引：3，自引：0，他引：3  

李猛  郭卫东  夏恩琴  韩宇超  胡明辉 《热带海洋学报》2006,25(1):9-14

研究了厦门湾九龙江河口区、西海域、同安湾及东侧水道海水中有色溶解有机物(CDOM)的光吸收特性,分析了CDOM的河口行为,并讨论了CDOM光吸收特性与其荧光性质之间的关系。结果表明,厦门湾表层海水CDOM光吸收系数a(355)的水平分布表现为河口区最高、东侧水道最低、西海域和同安湾介于两者之间,底层水a(355)的分布与表层基本相似,表明陆源河流输入是厦门湾CDOM的主要来源;a(355)的垂直分布为表层高于底层,主要受水文和生物因素控制。厦门湾表层水CDOM光谱斜率S的平均值介于0.014—0.018nm-1,但河口低盐度区S值较小,反映陆源腐殖质的影响。a(355)在河口混合中呈保守行为,表明CDOM具有良好的保守性质。CDOM的吸收系数a(355)与其荧光强度之间表现为较好的相关关系,指示可以用灵敏度更高的荧光方法来研究CDOM的分布和行为。  相似文献   

水中矿物油荧光特性实验研究     

夏达英  张士魁  李宝华  王振先  孙培光  史锦珊  尚丽平 《海洋科学进展》2002,20(2):91-98

为了实现现场测量地表水中矿物油含量的全光纤荧光计的研制。采用荧光光谱测量分析方法 ,于 1 999年 1 0月— 2 0 0 0年 5月 ,采集了国内外不同的地区原油和成品油共 2 3种 ,用1 4种不同波长的激发光激励 ,进行荧光光谱特性测量实验研究。同时 ,对不同浓度的矿物油、水混合液也进行了测量实验 ,得出其最低检测浓度为 0 .0 0 5mg/L ;测量了青岛近岸海域表层受油污染海水的含油量为 0 .0 1～ 0 .2 2mg/L ;此外对水中矿物油受激荧光发射原理也作了简述。并对水中矿物油荧光光谱特性、浓度与荧光强度的关系 ,以及为研制现场测量水中矿物油浓度的全光纤荧光计的可能性作出了结论性的意见  相似文献   

Occurrence of Organic Matter in Calcimicrobialites across Permian-Triassic Boundary in Huayingshan Region, Sichuan, South China     

杨浩  王永标  陈林 《中国地质大学学报(英文版)》2008,19(5)

Calcimicrobialites across the Permian-Triassic boundary in Huayingshan (华蓥山) region were investigated using the fluorescence microscopic measurements to understand the occurrence of organic matter. The microbialites are composed of micrite matrix and coarse spar cement. Abundant rhombic or magnetic needle-like carbonate minerals were observed adrift within the cement. The fluorescence microscopic measurement indicates the micrite matrix in microbialites shows the most abundant organic matter, with the rhombic or magnetic needle-like carbonate minerals and coarse spar cement coming to the 2nd and the 3rd, respectively. Organic matter is mainly preserved in the space between the grains of the micrite minerals but almost evenly distributed in the rhombic or magnetic needle-like carbonate minerals. As one of the common diagenesis types, dolomitization is observed to occur in the microbialites in Huayingshan. However, the carbonate cement in microbialites still has high content of element Sr as shown by the microprobe analysis, reflecting that the dolomitization might have happened in a restricted environment. Observation under the fluorescence microscope shows that dolomitization just led to the redistribution of organic matter in the grain space of dolomite minerals, inferring that the diagenesis has a slight effect on the preservation, and thus on the content of organic matter in the microbialites.  相似文献   

影响荧光光谱分析质量的因素   总被引：2，自引：1，他引：1  

宁丽荣  沈洪久  李武  张庆珍  王波舫 《物探与化探》2008,32(6)

荧光光谱分析是油气化探中非常重要的一种分析方法,因此在地表样品的分析中,荧光光谱质量就显得尤其重要。消除影响因素,保证分析质量,是得到真实的地下油气信息的必要条件。通过实验研究,对影响荧光光谱分析质量的因素的进行探讨,除了在分析过程出现的影响因素外,样品的包装袋也是造成影响的重要因素。  相似文献