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151.
H. Jonsson K. Viken Sandnes D. Schiedek R. Schneider B. E. Grsvik A. Goksyr 《Marine environmental research》2004,58(2-5):655
In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of ≈49 kDa size, and weak signals for proteins of ≈41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of ≈56 and 59 kDa size, and also a weak immunoreaction with a protein of ≈43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14–40 ng/g DW in controls to 373–638 ng/g DW in exposed mussels. 相似文献
152.
在实验室内, 22. 5±0. 5℃条件下以去头去骨去鳞片去皮的金色小沙丁鱼的纯肉糜为饵料饲养斜带髭鲷幼鱼,测定体重范围在 4. 6~6. 5g的斜带髭鲷的体重、体长的生长曲线及其生态转换效率和特定生长率.实验结果表明,斜带髭鲷的体重、体长在实验期间直线增长,体重、体长的生长曲线分别为Wt(w.w.湿重,下同 ) =0. 391 7t 5. 766 2(R=0. 999 6)和Lt=0. 105t 6. 265(R=0. 989 9).用胃含物法测定斜带髭鲷的胃排空率、日摄食量、日生长量、生态转换效率和特定生长率.实验得到斜带髭鲷消化道的瞬时排空率Rt= 1. 23 4×10-3 (m/m) /h,日摄食量Cd= 1. 342 9g(w.w. ),生态转换效率Eg=29. 50% ±2. 77% [m/m(w.w. ) ],特定生长率SGR=4. 71%±0. 69% [m/m(w.w. ) ]. 相似文献
153.
海底管道开沟机械的技术经济性能评定与选型 总被引:1,自引:0,他引:1
本文在对水下管道开沟机械的需求市场、实际应用和研制开发动向作深入调研的基础上,选择水下管道开沟机械特有的技术先进性、适用性、安全可靠性以及经济性评价指标,构造评价模型,对水下管道施工作业中广为应用的水力冲射开沟机械、海底管道犁、机械开沟机和土壤液化法埋管设备及其机型的技术经济性能作了较为详尽地分析对比及综合评价。在此基础上,笔者提出了水下管道开沟机械的一般选型原则和对我国海底管道开沟机械研究开发工 相似文献
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本文揭示了天津海岸带影响经济开发的四个方面的地质环境特点;着重探讨三个方面的环境工程地质问题;并防止其形成和减轻地灾害的对策进行了研究。 相似文献
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The Eastern Flank Hydrocarbon Province borders the southeastern edge of the South Oman Salt Basin in the southern part of Oman. An intensive exploration programme by PDO over the past seven years has led to the discovery of almost 2 × 109 m3 of oil with current production of some 15000 m3/d from six fields.In stark contrast to other oil habitats of the Arabian Peninsula, the main play involves rock units and geological events ranging in age from Late Precambrian to Quaternary Times, while trap development and charging are closely related to syn-tectonic salt dissolution.The principal reservoirs and seals are poorly consolidated, Palaeozoic clastics which drape, due to salt dissolution, over residual ‘cores’ principally composed of either Lower Palaeozoic sandstones (Haima Group), or Late Proterozoic carbonates (Huqf Group), or some combination of these. Oils have been geochemically correlated with algal source rocks of the Huqf Eastern Flank. Structures are considered to have been ‘charged’ from Late Mesozoic times onwards in conjunction with progressive subsurface salt removal and the onset of maturation of local source rocks. The effectiveness of ‘charging’ along the retreating salt edge is indicated by the small percentage of dry wells.Major oil zones are found in both anticlinal and truncation traps which are commonly developed around sandstone (Haima Group) cores. Oils show a wide range of characteristics but are typically heavy, early expulsion crudes, high sulphur with little associated gas. Bacterial transformation is not uncommon.Producing reservoirs mainly occur at relatively shallow depths (600–1200 m). Primary recovery factors of around 7% reflect the high density and viscosity of the Eastern Flank crudes and have initiated interest in Enhanced Oil Recovery (EOR) techniques. Two such pilot projects, involving steam and polymer flooding, are due to commence in 1984 and, if successful, will substantially increase the reserve base of these fields. 相似文献