Particulate peptidoglycan in seawater determined by the silkworm larvae plasma (SLP) assay     

Akira Shibata  Kouichi Ohwada  Masakazu Tsuchiya  Kazuhiro Kogure 《Journal of Oceanography》2006,62(1):91-97

Peptidoglycan (PG) is a biopolymer found exclusively in the cell wall of bacteria. Recent chemical analysis of particulate organic matter suggests that a major amount of the muramic acid, an amino sugar present only in PG, could not be accounted for in terms of bacterial cells (Benner and Kaiser, 2003); however, data on particulate PG is quite sparse. In the present study, conducted in 1996, the PG was examined at 5 sampling sites in the northwestern Pacific Ocean, and in natural seawater cultures. Particulate PG, which was concentrated using a 96-well filtration plate equipped with Durapore filters (pore size, 0.22 μm), was measured by the silkworm larvae plasma (SLP) assay. The PG concentration generally decreased with depth and correlated significantly with bacterial abundance throughout the entire water column. However, the ratio of particulate PG to bacterial abundance varied with depth. The average ratio was 0.61 ± 0.53 (average ± SD, n = 40) between 50 and 2000 m, which agreed with the bacterial cellular PG content from 0.63 to 1.1 fg cell⁻¹ obtained in seawater cultures. On the other hand, the ratios of PG to bacteria from the surface to 50 m (3.7 ± 2.6, n = 29) and below 2,000 m (2.1 ± 1.7, n = 7) were significantly higher than that between 50 and 2,000 m. These results may suggest that, in the surface and deep layers, a significant fraction of particulate PG was present in bacterial detritus, whereas this fraction was reduced in the middle layer.  相似文献   

A case study of mollusc and polychaete soft-bottom assemblages submitted to sedimentary instability in the Mediterranean Sea     

Andrea Cosentino  & Salvatore Giacobbe 《Marine Ecology》2006,27(2):170-183

In the Strait of Messina (Sicily, Central Mediterranean Sea) strong tidal currents, very irregular hydrological regime and related solid load, and local seismic activity cause sediment instability; this area represents therefore a case‐study of a naturally disturbed soft‐bottom environment. In this paper, mollusc and polychaete assemblages of the northern part of the Strait were described. Composition, community structure, eco‐ethological features, trophic guilds and sediment‐type relationships were studied in 64 stations sampled located between 3.5 and 50 m depth in October 1992 by means of a Van Veen grab. A total of 131 species (65 molluscs and 66 polychaetes) were identified. A dense population of the tubicolous polychaete Ditrupa arietina was recognized, together with the occurrence of other species tied to a high sedimentation rate, such as Corbula gibba and Tellina distorta, as well as widespread, mud‐tolerant species (e.g.Chone spp., Hyalinoecia tubicola). Diversity (H′) showed a peak at intermediate depths (10/20 m) and a clear decrease beyond this depth, corresponding to the Ditrupa core population. Multivariate comparison between sediment features and community composition throughout the bathymetric gradient showed a narrow ecocline between two environments subjected to opposite hydrodynamic constraints. In the shallower zone, a wide typology of trophic‐ethological guilds was related to community patchiness, in contrast to a greater functional uniformity of the deeper assemblage, dominated by sessile, semi‐infaunal suspension feeders. A possible role played by a phase of increased rainfall to increase bottom instability, locally emphasized by a previous human activity, is also discussed.  相似文献   

双层局部开孔板沉箱对波浪反射的理论研究   总被引：1，自引：0，他引：1  

李玉成  刘洪杰滕斌 《海洋工程》2005,23(1):18-27,32

提出了一种用于研究由双层开孔板和一个不透水后板的开孔结构对斜向波反射率的理论分析方法。整个流域被分成三个子域，在每个子域内应用特征函数展开法以得到该域内包含未知展开系数的势函数的表达式，在速度势的展开中，考虑了非传播模态波浪的影响。通过匹配开孔板处的边界条件可以求解待定的展开系数，继而求解双层开孔板防波堤结构对斜向波的反射率。数值计算结果与试验结果进行了比较，符合较好。并进一步讨论了几个重要因素对反射系数的影响。  相似文献   

Flow fields in reflected waves at a sloped sea wall     

Gerald Müller   《Ocean Engineering》2007,34(11-12):1786-1789

Wave run-up, and flow visualization experiments were conducted with a 1:2 sloped sea wall model. The visualization experiments gave an overview of flow fields in reflected, non-breaking conditions. Maximum particle velocities were found to be significantly smaller than suggested in the literature. Downrush produced a fast sheet flow, extending down to the toe of the sea wall. This created a ‘reverse’ breaker during the retreat of the initially non-breaking wave, which explains the high-energy dissipation rates for non-breaking waves reported in the literature. Embankments may therefore be exposed to wave impact pressures in areas up to 1.18H₀ below MWL.  相似文献   

Stability of waterfront retaining wall subjected to pseudo-static earthquake forces     

Deepankar Choudhury  Syed Mohd. Ahmad 《Ocean Engineering》2007,34(14-15):1947-1954

Waterfront retaining walls supporting dry backfill are subjected to hydrostatic pressure on upstream face and earth pressure on the downstream face. Under seismic conditions, if such a wall retains a submerged backfill, additional hydrodynamic pressures are generated. This paper pertains to a study in which the effect of earthquakes along with the hydrodynamic pressure including inertial forces on such a retaining wall is observed. The hydrodynamic pressure is calculated using Westergaard's approach, while the earth pressure is calculated using Mononobe-Okabe's pseudo-static analysis. It is observed that when the horizontal seismic acceleration coefficient is increased from 0 to 0.2, there is a 57% decrease in the factor of safety of the retaining wall in sliding mode. For investigating the effect of different parameters, a parametric study is also done. It is observed that if φ is increased from 30° to 35°, there is an increase in the factor of safety in the sliding mode by 20.4%. Similar observations were made for other parameters as well. Comparison of results obtained from the present approach with [Ebeling, R.M., Morrison Jr, E.E., 1992. The seismic design of waterfront retaining structures. US Army Technical Report ITL-92-11. Washington DC] reveal that the factor of safety for static condition (k_h=0), calculated by both the approaches, is 1.60 while for an earthquake with k_h=0.2, they differ by 22.5% due to the consideration of wall inertia in the present study.  相似文献   

Instrumentation and Numerical Analysis of Cylindrical Diaphragm Wall Movement During Deep Excavation at Coastal Area     

Dong Soo Kim  Byoung Chul Lee 《Marine Georesources & Geotechnology》2005,23(1):117-136

The lateral deflection of a cylindrical diaphragm wall and the associated ground movement induced by deep excavation are analyzed by performing site instrumentations and numerical analyses in the coastal area of Korea. Wall lateral deflection, rebar stress, and pore water pressure were measured and analyzed in eight directions. Variations of soil properties with the decrease of confining pressure are compared by performing various in situ tests before ad after excavation. To calculate the wall lateral deflection accurately, the effects of small strain nonlinearity, confining pressure, and the hysteresis loading/unloading loop developed during excavation are considered in the proposed numerical analysis. By comparing numerical results with measured ones, the importances of considering small strain nonlinearity and confining pressure reduction in the nonlinear (FEM) are emphasized. Also, the effects of wall stiffness on the performance of cylindrical diaphragm walls are studied for future similar excavation in the coastal area.  相似文献   

Wave-induced porewater pressure in a seabed with inhomogeneous permeability     

Toshikazu Kitano  Hajime Mase 《Ocean Engineering》2001,28(3)

We have developed an analytical treatment for the wave-induced response of a seabed with inhomogeneous permeability, by employing the boundary layer approximation. By assuming an exponential function for vertically distributed permeability, the inhomogeneous consolidation equation was formulated as Bessel's differential equation. Subsequently, we proposed some representative quantities for examining the effects of inhomogeneity of permeability. The main effects of inhomogeneity are that the boundary layer becomes thinner and the phase delay in the direction of depth becomes larger as the inhomogeneity becomes significant. In addition, it was shown that the boundary layer is properly evaluated by using the averaged permeability.  相似文献   

水平井含水率上升影响因素   总被引：1，自引：1，他引：0  

凌宗发  王丽娟  李保柱  夏静  朱玉新 《现代地质》2008,22(1):86-90

利用塔里木油田塔中4油田(TZ4)底水油藏相关的地质、流体数据建立数值模型。在所建模型的基础上,应用数值模拟计算的累积产油、产水和产液量回归俞启泰水驱特征曲线,以反映水平井见水特征的参数b。以参数b为研究对象,采用正交试验的方法研究不同因素对b值的影响,筛选影响水平井见水特征的主要因素,认为原油粘度、油层厚度、非均质性及水平井水平段在油藏中的位置是影响水平井含水上升趋势的主要指标。最后建立主要因素与b值的关系式,结合俞启泰曲线b值图版,提出预测水平井见水规律的公式——图版法(F-b法)。应用F-b法对塔里木水平井含水率进行预测,并与其他相关方法及实际生产数据对比,认为F-b法可作为预测塔里木油田水平井含水率、估算可采储量的一种有效方法。  相似文献   

塔东地区古城4井上寒武统—中、上奥陶统流体地球化学示踪     

王东  王国芝  冉启贵  刘树根  胡九珍  张长俊 《现代地质》2008,22(4)

通过对塔东地区古城4井上寒武统和中、上奥陶统碳酸盐岩围岩及充填物的碳、氧、锶同位素地球化学和流体包裹体成分的对比研究表明,充填于中、上奥陶统和上寒武统的流体分属于2个不同来源的流体体系。充填于中、上奥陶统灰岩裂缝中方解石脉的w(87Sr)/w(86Sr)介于0.7084~0.7088,它与早奥陶世海水的w(87Sr)/w(86Sr)相近;流体体系为CH4-H2O体系;充填物与围岩间具有明显的碳、氧同位素差异,表明上部流体体系中的流体来自于奥陶系地层本身。上寒武统白云岩裂缝中方解石脉的w(87Sr)/w(86Sr)为0.7138,明显地高于同时代海水的锶同位素值;流体体系为CO2-H2O体系;下部流体体系中的流体为外来富锶流体。上、下流体体系间互不连通暗示着上寒武统地层具有相对较好的保存条件。  相似文献   

低渗透储层水平井—直井联合布井极限注采井距研究     

侯晓春  陆程  蔡文  郑兴范 《现代地质》2008,22(1)

低渗透储层水平井—直井联合布井技术具有广泛的应用前景,其布井方式至关重要。首先利用劈分流场的方法直观地给出常规储层水平井—直井联合布井的产能评价方法;然后针对低渗透储层的渗流特点,在考虑启动压力梯度条件下建立新的井网渗流物理模型并求解得到产能评价公式;最后经分析计算给出启动压力梯度影响的面积井网极限注采井距关系曲线。研究结果为低渗透储层部署水平井—直井联合井网提供理论依据。  相似文献