三疣梭子蟹几丁质酶基因(PtCht6)的克隆及其在免疫中的功能分析     

宋柳  吕建建  王磊  孙东方  刘萍 《海洋与湖沼》2019,50(5):1080-1090

几丁质酶(chitinase)在甲壳动物的蜕皮、消化和免疫等很多生物学过程中发挥重要功能,为深入探讨几丁质酶的免疫防御机制,本实验利用RACE技术克隆了三疣梭子蟹(Portunus trituberculatus)PtCht6基因。该基因cDNA全长2736bp,开放阅读框(ORF)2103bp,编码700个氨基酸,具有几丁质酶GH18家族典型特征。利用实时荧光定量技术分析PtCht6在三疣梭子蟹不同组织、不同蜕皮阶段、不同病原感染以及低盐胁迫(11)下的表达特征,结果显示:PtCht6在各组织中均有表达且在肝胰腺中的表达量最高;在不同蜕皮时期的肝胰腺中,其表达量由蜕皮后期(A/B)、蜕皮间期(C)、蜕皮前期(D)依次递减(P0.05);人工感染WSSV和副溶血弧菌后.PtCht6的表达量在肝胰腺中分别于12h、72h达到最大值,在血细胞中均于12h达到最大值,且相较于对照组,整体显著上调表达(P0.05);低盐胁迫能够显著抑制该基因的表达,最高抑制73倍(P0.05)。同时发现,低盐环境下感染WSSV后,该基因达到峰值的时间明显延迟(至少延迟12h.P0.05)。该研究结果预示PtCht6作为免疫因子参与三疣梭子蟹的病原防御,且低盐胁迫在一定程度上抑制了该基因的免疫功能。  相似文献   

海洋水色卫星遥感研究与进展   总被引：7，自引：3，他引：7  

李四海  王宏 《地球科学进展》2000,15(2):190-196

系统介绍了目前海洋水色卫星遥感在海洋碳通量、生物海洋学及上层海洋过程研究,以及海岸带环境监测与管理中的主要应用方向;分析了上述应用对水色卫星传感器在时空分辨率、光谱波段设置和空间覆盖范围等方面的具体设计要求;概括了新一代水色卫星传感器的特点;指出了水色卫星遥感资料应用中的定标、大气校正及定量反演技术等关键问题;介绍了各国海洋水色卫星遥感的发展计划,提出了我国海洋水色卫星遥感研究的对策。  相似文献   

The new magnetic / non-magnetic double degenerate system EUVE J1439+75.0     

Stéphane Vennes  Lilia Ferrario  Dayal T. Wickramasinghe 《Monthly notices of the Royal Astronomical Society》1999,302(3):L49-L52

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A survey for cool white dwarfs and the age of the Galactic disc     

R. A. Knox  M. R. S. Hawkins  N. C. Hambly 《Monthly notices of the Royal Astronomical Society》1999,306(3):736-752

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On the parallax of WD 0346++246: a halo white dwarf candidate     

N. C. Hambly  S. J. Smartt  S. T. Hodgkin  R. F. Jameson  S. N. Kemp  W. R. J. Rolleston  I. A. Steele 《Monthly notices of the Royal Astronomical Society》1999,309(4):L33-L36

We present a parallax measurement for the very cool degenerate WD 0346+246, the serendipitous discovery of which was reported by Hambly et al. We find an absolute parallax of 36±5 mas, yielding a distance estimate of 28±4 pc. The resulting absolute visual magnitude of the object is M _V =16.8±0.3, making it the second-lowest luminosity white dwarf currently known. We use the distance estimate and measured proper motion to show that the object has kinematics consistent with membership of the Galactic halo. WD 0346+246 is therefore by far the coolest and least luminous of only a handful of plausible halo white dwarf candidates. As such, the object has relevance to the ongoing debate concerning the results of microlensing experiments and the nature of any baryonic dark matter component to the Galactic halo residing in stellar remnants.  相似文献   

A spatially resolved 'inside-out' outburst of IP Pegasi     

N. A. Webb  T. Naylor  Z. Ioannou  W. J. Worraker  J. Stull  A. Allan  R. Fried  N. D. James  D. Strange 《Monthly notices of the Royal Astronomical Society》1999,310(2):407-413

We present a comprehensive photometric data set taken over the entire outburst of the eclipsing dwarf nova IP Peg in 1997 September/October. Analysis of the light curves taken over the long rise to the peak-of-outburst shows conclusively that the outburst started near the centre of the disc and moved outwards. This is the first data set that spatially resolves such an outburst. The data set is consistent with the idea that long rise times are indicative of such 'inside-out' outbursts. We show how the thickness and the radius of the disc, along with the mass transfer rate, change over the whole outburst. In addition, we show evidence of the secondary and the irradiation thereof. We discuss the possibility of spiral shocks in the disc; however, we find no conclusive evidence of their existence in this data set.  相似文献   

Temporal and spatial trends of lake acidity in northern Sweden     

Tom Korsman 《Journal of Paleolimnology》1999,22(1):1-15

Changes in lake-water pH, alkalinity and colour were inferred from diatoms in surface sediment samples and sediment samples from pre-industrial times from 118 northern Swedish lakes. This palaeolimnological study does not support the hypothesis that there is a large-scale modern acidification in the two northernmost counties of Sweden; pH had decreased significantly in eight lakes, while five had a significant increase. Partial least-squares regression of changes in water chemistry in relation to catchment characteristics was performed to evaluate the causes of the acidity status. Furthermore, temporal trends were assessed from long sediment cores from five acidic lakes. The results suggest that the presently acid lakes have faced a long-term acidification trend over several thousand years due to soil-forming processes and vegetation development. However, due to the acid sensitivity of the region, future acidification trends in northern Swedish lakes should be carefully observed and assessed.  相似文献   

Evidence for the stratification of Fe in the photosphere of G191-B2B     

M. A. Barstow  I. Hubeny  J. B. Holberg 《Monthly notices of the Royal Astronomical Society》1999,307(4):884-894

The presence of heavy elements in the atmospheres of the hottest H-rich DA white dwarfs has been the subject of considerable interest. While theoretical calculations can demonstrate that radiative forces, counteracting the effects of gravitational settling, can explain the detections of individual species, the predicted abundances do not accord well with observation. However, accurate abundance measurements can only be based on a thorough understanding of the physical structure of the white dwarf photospheres, which has proved elusive. Recently, the availability of new non-local thermodynamic equilibrium model atmospheres with improved atomic data has allowed self-consistent analysis of the extreme ultraviolet (EUV), far UV and optical spectra of the prototypical object G191-B2B. Even so, the predicted and observed stellar fluxes remain in serious disagreement at the shortest wavelengths (below ≈190 Å), while the inferred abundances remain largely unaltered. We show here that the complete spectrum of G191-B2B can be explained by a model atmosphere where Fe is stratified, with increasing abundance at greater depth. This abundance profile may explain the difficulties in matching observed photospheric abundances, usually obtained by analyses using homogeneous model atmospheres, to the detailed radiative levitation predictions, particularly as the latter are only strictly valid for regions deeper than where the EUV/far UV lines and continua are formed. Furthermore, the relative depletion of Fe in the outer layers of the atmosphere may be evidence for radiatively driven mass-loss in G191-B2B.  相似文献   

Ecological and Life History Characteristics of Botryllus schlosseri Tunicata Populations Inhabiting Undersurface Shallow-Water Stones     

B. Rinkevich  R. Porat  M. Goren 《Marine Ecology》1998,19(2):129-145

Abstract. Populations of Botryllus schlosseri PALLAS 1772. a cosmopolitan colonial ascidian. were examined on the undersurface of stones for the first time in three adjoining localities along the Israeli Mediterranean coast. A two-year study of 1589 stones which were inhabited by 1345 colonies, revealed that colony coverage was < 1.5 % of the total available substrate area; values were highest in spring, lowest in winter. The two years differed significantly in the number of colonies · m ², and these numbers were correlated with mean seawater temperatures. Most of the stones contained 1–5 Botryllus colonies year-round; no significant correlation was found between number and stone size. The brown morph was dominant 80 % at all three localities. The distribution of other colour morphs differed between localities. Reproductive colonies were either hermaphroditic or contained male gonads only. Peak reproduction was in the spring, but did not correlate with seawater temperatures. While colony size ranged between I and 1I55zooids, sexually mature colonies consisted of 171–273 zooids on the average, compared with 37–90 zooids for sterile colonies. The three populations differed significantly in several ecological and life history characteristics. This further confirmed past studies indicating that Botryllus populations are characteristically divided into local subpopulations exhibiting microgeographic differences in life history patterns. The results are compared with the accumulated data on other world-wide Botryllus populations residing in other habitats.  相似文献   

Commuting activity spaces of married women in a suburb of the Tokyo metropolitan area     

Masaki Kawase 《GeoJournal》1999,48(3):217-222

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