Design of Vibrio 16S rRNA Gene Specific Primers and Their Application in the Analysis of Seawater Vibrio Community     

LIU Yong YANG Guanpin WANG Hualei CHEN Jixiang SHI Xianming ZOU Guiwei WEI Qiwei SUN Xiuqin 《中国海洋大学学报(英文版)》2006,5(2):157-164

The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and consumption is of great importance for food and mariculture safety. In order to enrich Vibrio-representing 16S ribosomal RNA gene （rDNA） fragments and identify these bacteria further real-timely and synchronously among bacterial flora in the chain, a pair of primers that selectively amplify Vibrio 16S rDNA fragments were designed with their specificities and coverage testified in the analysis of seawater Vibrio community. The specificities and coverage of two primers, VF169 and VR744, were determined theoretically among bacterial 16S rDNAs available in GenBank by using BLAST program and practically by amplifying Vibrio 16S rDNA fragments from seawater DNA. More than 88.3% of sequences in GenBank, which showed identical matches with VR744, belong to Vibrio genus. A total of 33 clones were randomly selected and sequenced. All of the sequences showed their highest similarities to and clustered around those of diverse known Vibrio species. The primers designed are capable of retrieving a wide range of Vibrio 16S rDNA fragments specifically among bacterial flora in seawater, the most important natural environment of seafood cultivation.  相似文献   

口腔细菌的妙用     

《海洋世界》2008,(12):9-9

瑞士科学家最近报道了人类口腔细菌在产生食物独特味道中发挥的作用。科学家在之前的研究中指出，一些蔬菜和水果只有在入口之后才会释放出独特的气味。在这个过程中，许多挥发性的化合物由其前体转化而成。这次，研究者发现，人类口腔中的一种厌氧菌在转化过程中发挥了重要作用，促使了气味的生成。研究者解释说，  相似文献   

Screening and molecular classification of low-temperature protease from Antarctic microorganism and its characterization     

王全富缪锦来  侯艳华郑洲 李光友 《极地研究(英文版)》2005,16(2):81-89

107 strains producing protease were screened from 260 strains of Antarctic psychrophilic bacteria, among which proteolytic activity of five strains was more than 45 U ml^-1. The 16S rRNA gcne sequences homology and phylogcnetic analysis of five Antarctic psychrophillc bacteria showed that NJ276, NJS-9, NJ16-70,NJ345 belonged tO the described genus Pseudoalteromonas and NJ341 belonged to the genus Colwellia. The growth and the protease characteristic of four Antarctic psychrophilic bacteria had been studied, and the result showed that the 6ptimal temperature for growth and protease-produeing of four strains was about 10℃. Their growth and protease-produeing were still high during incubatlng 2-5 days. The maximum proteolytic activity occurred at pH 9 for four Antarctic psychrophilic bacteria. The optimal temperature of protease action of both strains NJ276 and NJ5-9 was about 50℃, however, the optimal temperature of protease aetlon of both strains NJ341 and NJ345 was about 40 ℃, and their proteolytic activity under 0℃ exhibited nearly 30% of the maximum activity, but their thermal stabilities were weaker. These results indicated that proteases from NJ341 and NJ345 were low-temperature proteases.  相似文献   

GPS共视法时间比对在陕西天文台的建立     

吴贵臣  和翠英  董绍武 《时间频率学报》1993,(2)

利用国产GPS定时型接收机,按照国际共视规程,陕西天文台建立了GPS共视法时间比对.研究了影响比对精度的因素,实现了微机控制的数据自动采集和处理,实现了国际间的全面共视比对.1991年3月起,BIPM已正式用于国际原子时TAI的计算中。  相似文献   

四川省510铀矿床中铀及伴生元素的分带特征     

王永飞  董之凯  吕文祥  李宝新  马冰 《物探与化探》2023,(4):881-891

四川省510铀矿床伴生元素的研究多为单一矿段,为划分整个矿床的伴生资源类型,本次采用铀矿石伴生元素横向及垂向分带研究的方法,结合矿床已有的岩石地球化学、矿床地球化学、成矿规律等研究成果进行综合对比分析,梳理整个矿床伴生矿产的分带特征,将矿床在垂向上划分为铁镍硫化物—铀矿化带、少硫化物—铀矿化带和闪锌矿—少铁镍硫化物—铀矿化带3个矿物组合带,在横向上划分为上部Ni、V→Zn→Ni(少量Cu)和下部Mo、Ni、Zn、V→Zn、Ni→Mo、Ni、Zn→Zn(少量Cu)的成矿元素组合过渡带。矿床中铀与伴生钼、镍和锌等金属已完成冶炼及回收实验,增加了矿床的经济价值,扩大了经济效益。本次研究确定了510铀矿床6种伴生资源类型,对伴生元素的利用进行了综合评价,为深部找矿预测及相似矿山接替资源找矿方向提供了理论指导。  相似文献   

西藏纳木错沿岸表层水体浮游细菌群落结构及生态功能预测北大核心CSCD     

孟华旦尚  薛曌  郭小芳  德吉 《冰川冻土》2023,(1):254-266

浮游细菌群落对高原湖泊变化具有高度响应性,并且会影响高原湖泊生境的地球化学平衡。因此,了解高原湖泊中浮游细菌群落的分布特征,阐明其在高原湖泊生态系统中的生态功能具有重要科学意义。2021年5月对纳木错沿岸浮游细菌群落分布特征进行了调查研究,并采用16S rDNA高通量测序技术对样品进行分析,通过α-多样性指数分析浮游细菌群落的差异性,通过共现网络分析浮游细菌群落之间的相互作用,利用Pearson相关系数衡量理化因子与α-多样性指数的相关性,采用冗余分析(RDA)探讨水体理化因子对浮游细菌群落结构的影响,并基于PICRUSTt2对纳木错浮游细菌进行功能预测。结果表明:浮游细菌群落主要由变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)、蓝细菌门(Cyanobacteria)和厚壁菌门(Firmicutes)组成,其中变形菌门相对丰度最高,主要包括γ-变形菌纲(Gamma-proteobacteria)和α-变形菌纲(Alpha-proteobacteria);噬氢菌属(Hydrogenophaga)和嗜冷菌属(Algoriphagus)为相对优势菌属。α-多样性指数表明,纳木错浮游细菌群落比较丰富。共现网络节点间关系以正相关为主;总溶解固体量(TDS)和盐度(Sal)是影响纳木错浮游细菌群落结构的关键因子。功能预测结果显示,纳木错浮游细菌群落功能主要涉及代谢、遗传信息处理、环境信息处理等6类生物代谢通路,以及膜运输、氨基酸代谢、碳水化合物代谢等46个子功能。综上所述,纳木错浮游细菌群落结构在各样点间存在一定差异,浮游细菌在门级水平上类群间相互作用主要为协同作用,其群落结构是多个因子共同作用的结果。研究阐明了纳木错浮游细菌群落组成和功能及其与环境因子的相互联系,可为当地生态环境保护提供科学依据。  相似文献   

Isolation and biological characteristics of aerobic marine magnetotactic bacterium YSC-1     

高峻潘红苗  岳海东宋涛 赵勇陈冠军  吴龙飞肖天 《中国海洋湖沼学报》2006,24(4):358-363

1 INTRODUCTION Magnetotactic bacteria are able to respond and orient along the direction of terrestrial or artificial magnetic field, a behavior referred to as magneto- taxis. In the majority of magnetotactic bacteria, the magnetosomes are 25–120 nm in d…  相似文献   

亚洲一号卫星共视法定轨及时刻同步     

宋金安 《时间频率学报》1995,18(6):10-19

１９９３年中国科学院同步卫星观测网同步接收亚洲一号卫星的电视信号，进行了多台站时差法定轨及共视法时刻同步试验。试验：时差法定轨在经度、纬度方向精度较高，在卫星高度方向误差较大，不进行卫星位置修正时，时刻同步误差小于３μｓ； 卫星位置修正时的时刻同步误差小于０．９μｓ，可应用于局或大范围网络时刻同步及同步卫星轨道研究。  相似文献   

亚洲一号卫星共视法定轨及时刻同步     

Song Jinan 《时间频率学报》1995,(1)

１９９３年中国科学院同步卫星观测网同步接收亚洲一号卫星的电视信号，进行了多台站时差法定轨及共视法时刻同步试验。试验表明：时差法定轨在经度、纬度方向精度较高，在卫星高度方向误差较大。不进行卫星位置修正时，时刻同步误差小于３μｓ；进行卫星位置修正时的时刻同步误差小于０．９μｓ，可应用于局部或大范围网络时刻同步及同步卫星轨道研究。  相似文献   

Distribution of biomass of heterotrophic bacterioplankton in the Bohai Sea     

白洁李岿然  张经李正炎  高会旺张昊飞 《中国海洋湖沼学报》2005,23(4):427-432

Distribution, variation and impact factors of biomass of bacterioplankton from April to May 1999 in Bohai Sea were studied in DAPI method with epifluorescence microscopy. The biomass in surface waters showed a small day-night variation, varying from 0.13 to 2.51μg/dm^3 with an average of 0.84μg/dm^3. The biomass in bottom waters showed, however, a large variation, changing from 0.15 to 4.18μg/dm^3 with an average of 1.36μg/dm^3. The peak values occurred at 5 and 11 a.m. The bottom water biomass showed a significant correlation with particulate organic carbon （r=0.639, P〈0.05）. Heterotrophic bacterioplankton biomass was high in nearshore waters and low in offshore areas with a high biomass zone around Huanghe （Yellow） River mouth, showing the same distribution of nutrients. In vertical distribution, heterotrophic bacteria biomass in bottom waters was higher than that in surface water.  相似文献