大黄鱼(Pseudosciaena crocea)精子冷冻前后的活力及超微结构变化     

程顺  闫家强  竺俊全  姜建湖  吴雄飞  史会来 《海洋与湖沼》2013,44(1):56-61

采用两步降温法超低温冷冻保存大黄鱼精子,并用透射电镜技术研究了精子的超微结构损伤.结果表明,大黄鱼冻精的激活率、运动时间及寿命与鲜精相比无显著差异.鲜精中28.5％的精子形态结构异常,冻精中37％的精子形态结构异常.形态结构正常的精子表现为质膜与核膜结构完整、无膨胀现象,袖套、轴丝及中心粒结构正常,线粒体形态完整、嵴较发达;形态结构异常的精子表现为质膜破裂、脱落,质膜膨胀,核膜破裂、脱落,核局部受损伤,线粒体膨胀、嵴退化或消失,线粒体移位或脱落.结果显示,以Cortland溶液为稀释液,10％ DMSO为抗冻剂,对大黄鱼精子具有较好的抗冻保护作用.  相似文献   

大黄鱼(Larimichthys crocea)I型干扰素基因的特征与表达分析     

李婵  姚翠鸾 《海洋与湖沼》2013,44(2):499-506

采用RT-PCR和RACE-PCR技术克隆了大黄鱼(Larimichthys crocea)Ⅰ型干扰素基因全长cDNA序列及其基因组序列,并利用荧光定量PCR技术研究了该基因在大黄鱼不同组织中的表达谱,以及副溶血弧菌(Vibrio parahaemolyticus)、LPS、polyI:C刺激后大黄鱼脾脏、肝脏和头肾组织中该基因在转录水平的表达变化。结果表明,大黄鱼Ⅰ型IFN基因全长cDNA为878bp,开放阅读框558bp,编码185个氨基酸。推测的大黄鱼Ⅰ型IFN氨基酸序列N端含有20个氨基酸的信号肽,其后包含一个保守的IFabd结构域。大黄鱼Ⅰ型IFN基因包含5个外显子,4个内含子,在大多数组织和细胞中都有表达,其中在血细胞中表达量最高,肌肉中表达量最低。PolyI:C刺激可诱导大黄鱼Ⅰ型干扰素基因在脾脏、头肾和肝脏中的表达量显著上调;LPS刺激可诱导大黄鱼Ⅰ型干扰素基因在脾脏和肝脏中的表达量显著上调;灭活的副溶血弧菌可诱导大黄鱼Ⅰ型干扰素基因在头肾和肝脏中的表达量显著上调。  相似文献   

大黄鱼STAT6分子特征及其在溶藻弧菌感染后表达模式研究     

袁晓琴  陈友  荣毅  孟钰帆  任超群  母尹楠  陈新华 《海洋与湖沼》2022,53(5):1151-1160

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大黄鱼溶藻弧菌病组织中巨噬细胞移动抑制因子的检测及意义     

桑本红  徐晓津  毛勇  王军  苏永全 《海洋学报》2014,36(8):111-116

本文应用免疫组织化学方法,对健康大黄鱼和患溶藻弧菌病大黄鱼组织中巨噬细胞移动抑制因子(macrophage migration inhibitory factor,MIF)蛋白进行了检测,以研究溶藻弧菌病大黄鱼组织中MIF的表达情况及其对预后的影响。结果表明,MIF在健康大黄鱼头肾、脾、肠各组织中均无表达;MIF在溶藻弧菌病大黄鱼各组织中高表达,表达强度从弱到强依次是肠、脾、头肾,MIF表达阳性率分别为:56%、64%、92%。推测MIF在大黄鱼溶藻弧菌病发病中发挥重要作用。  相似文献   

盐度对大菱鲆幼鱼肌肉氨基酸和脂肪酸组成的影响     

曾霖  雷霁霖  刘滨  洪万树  艾春香 《海洋科学》2014,38(12):40-47

为了探讨盐度对幼鱼肌肉中氨基酸和脂肪酸组成的影响,作者将平均体质量为(7.16±0.07)g的大菱鲆(Scophthal musmaximus)幼鱼分别饲养在盐度(12、18、24、30(对照组)和36)水体中60 d。结果表明,不同盐度下,幼鱼肌肉中的氨基酸总量(TAA)、必需氨基酸(EAA)总量、非必需氨基(NEAA)总量和呈味氨基酸(FAA)总量分别在69.25%±0.37%~74.85%±1.26%、31.70%±0.11%~33.74%±0.28%、31.29%±0.44%~34.60%±1.26%和24.20%±0.66%~26.98%±1.02%,从高到低依次为36组30组12组18组24组,36组均显著高于24组(P0.05)。支链氨基酸(BCAA)总量、EAA/TAA、EAA/NEAA、FAA/TAA分别在14.94%±0.34%~15.37%±0.18%、44.57%±1.20%~45.78%±0.26%、80.40%±3.93%~84.42%±0.89%和34.95%±0.70%~36.41%±1.33%,各盐度组间均无显著性差异(P0.05)。必需氨基酸指数(EAAI)在40.41±1.23~44.46±0.54,30和36组显著大于其他组(P0.05)。脂肪酸(TFA)总量和非必需脂肪酸(NEFA)总量分别在88.65%±0.68%~89.66%±0.32%和85.11%±0.78%~86.38%±0.37%,12和30组均显著高于18组(P0.05)。多不饱和脂肪酸(PUFAS)总量和EPA+DHA总量分别在34.95%±1.01%~36.88%±0.64%和23.53%±1.30%~25.74%±0.25%,从高到低依次为36组30组24组12组18组,各盐度组间均无显著性差异(P0.05)。必需脂肪酸(EFA)总量在3.28%±0.04%~3.58%±0.10%,各盐度组间均无显著性差异(P0.05)。饱和脂肪酸(SFA)总量在27.51%±0.37%~29.83%±0.35%,12组显著高于36组(P0.05)。与对照组相比,36组大菱鲆幼鱼肌肉中的氨基酸和脂肪酸组成有所改善,其蛋白质和脂肪质量得到提高。研究成果可为大菱鲆渗透压调节的能量代谢机制及肌肉营养品质的环境调节机理积累基础资料。  相似文献   

温度和体质量对胭脂鱼鱼种耗氧率的影响     

许勤智  周波  陈娜娜  沈正雄  樊均德 《广东海洋大学学报》2014,(1):37-41

采用流水式的密封装置测定了胭脂鱼鱼种在18~30℃温度范围内的耗氧率,结果表明,在实验温度范围内,胭脂鱼鱼种耗氧率随温度上升逐渐升高,符合函数关系:y=-0.000 8 x2+0.044 0 x-0.438 1,R2=0.990 6。胭脂鱼鱼种耗氧率昼夜变化测定实验表明,其昼均耗氧率(0.204 2±0.011 0)mg?g-1?h-1,略低于夜均耗氧率(0.205 7±0.012 2)mg?g-1?h-1,二者差异不具统计学意义(P>0.05),表明胭脂鱼鱼种耗氧率并不存在明显的昼夜节律。在不同实验温度下,随着胭脂鱼鱼种规格的增大,其耗氧率均呈下降趋势。  相似文献   

高温对吉富罗非鱼幼鱼生长及超氧化物歧化酶、乳酸脱氢酶活力的影响     

杨洪帅  王辉  刘加慧  梁国栋 《广东海洋大学学报》2014,(1):15-20

研究34℃高温环境下吉富品系罗非鱼(GIFT Oreochromis niloticus)幼鱼生长及超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)活力的变化规律,以及SOD、LDH活力与幼鱼生长间的关系。结果表明:持续高温对幼鱼特定生长率及SOD、LDH活力的影响有统计学意义(P<0.05);随着时间的延长,LDH活力显著提高,差异有统计学意义(P<0.05),特定生长率及SOD活力随时间变化不具统计学意义(P>0.05);LDH活力增强是高温条件下幼鱼特定生长率降低的原因之一。  相似文献   

Ontogenetic development in the morphology and behavior of loach （Misgurnus anguillicaudatus） during early life stages     

高雷  段明  程飞  谢松光 《中国海洋湖沼学报》2014,32(5):973-981

Loach （Misgurnus anguillicaudatus） is a commercially important fish in China and an ideal aquaculture species. However, culturists experience high larval and juvenile mortality during mass production. To provide insight into ways to improve larviculture techniques, we describe the morphological characteristics and behavior of loach during the larval and early juvenile stages. Yolksae larvae ranged from 2.8 to 4.0 mm body length （BL） between days 0 to 4; preflexion larvae ranged from 3.6 to 5.5 mm BL between days 4 to 6; flexion larvae ranged from 4.8 to 8.1 mm BL between days 5 and 14; and post-flexion larvae ranged from 7. l to 15.7 mm BL between days 11 to 27; the minimum length and age of juveniles was 14.1 mm BL and 23 d, respectively. Loach are demersal from hatch through to the early juvenile stages. A suite of morphological characteristics （e.g., external gill filament and ventral mouth opening） and behavioral traits have developed to adapt to demersal living. We observed positive allometric growth in eye diameter, head length, head height, and pectoral fin length during the early larval stages, reflecting the priorities in the development of the organs essential for survival. Our results provide a basis for developing techniques to improve the survival of larval and juvenile loach during mass production.  相似文献   

Effect of Dietary Olaquindox on the Growth of Large Yellow Croaker（Pseudosciaena crocea R.） and the Distribution of Its Residues in Fish Tissues     

LI Huitao  ;WANG Weifang  ;MAI Kangsen  ;AI Qinghui  ;ZHANG Chunxiao  ;ZHANG Lu 《青岛海洋大学学报(英文版)》2014,(5):820-824

Olaquindox（OLA）, one of quinoxaline-N, N-dioxides, has been put under ban. However it was used as a medicinal feed additive early; it promotes the growth of livestock and prevents them from dysentery and bacterial enteritis. In this study, we evaluated the effect of dietary OLA on the growth of large yellow croaker（Pseudosciaena crocea R.） and the histological distribution of OLA and its metabolite 3-methyl-quinoxaline-2-carboxylic acid（MQCA） in fish tissues. Four diets containing 0（control）, 42.5, 89.5 and 277.2 mg kg^-1 OLA, respectively, were formulated and tested, 3 cages（1.0 m × 1.0 m × 1.5 m） each diet and 100 juveniles（9.75 ± 0.35 g） each cage. The fish were fed to satiation twice a day at 05：00 am and 17：00 pm for 8 weeks. The survival rate of fish fed the diet containing 42.5 and 89.5 mg kg^-1 OLA was significantly higher than that of fish fed the diet containing 0 and 277.2 mg kg^-1 OLA（P 0.05）, while the weight gain rate of fish fed the diet containing 42.5 and 89.5 mg kg^-1 OLA was significantly higher than that of fish fed the diet without OLA（control）（P0.05）, but similar to that of fish fed the diet with 277.2 mg kg^-1 OLA. Fish fed the diet with 277.2 mg kg^-1 OLA had the highest content of OLA and MQCA in liver（3.44 and 0.39 mg kg^-1, respectively）, skin（0.46 and 0.09 mg kg^-1, respectively） and muscle（0.24 and 0.06 mg kg^-1, respectively）. In average, fish fed the diet containing OLA had the highest content of OLA and MQCA in liver which was followed by skin and muscle（P〈 0.05）, whereas OLA and MQCA were not detectable in control. Our findings demonstrated that OLA and MQCA accumulated in large yellow croaker when it was fed with the diet containing OLA, thus imposing a potential safety risk to human health.  相似文献   

水中亚铁对大菱鲆幼鱼红细胞核异常及碱性磷酸酶和超氧化物歧化酶活性的影响     

吴志昊  尤锋  王英芳  文爱韵  马得友  徐永立  张培军 《海洋湖沼通报》2011,(4):45-51

本文比较研究了Fe(Ⅱ)浓度为0.01、0.05、0.1、0.5、1、2 mg/L及不同处理时间下大菱鲆幼鱼(Scophthalmus maximus)外周血红细胞核异常以及碱性磷酸酶(AKP)和超氧化物岐化酶(SOD)活力的变化.结果表明,在整个实验期间,o.01mg/L处理组的核异常细胞率及总核异常率均无明显变化....  相似文献