中华绒螯蟹血淋巴细胞钙激活钾通道研究     

段虎  王雪惠  孙金生 《海洋科学》2010,34(5):55-61

利用膜片钳技术之内面向外式,对培养2～24h不同类型的中华绒螯蟹(Eriocheirsinensis)血淋巴细胞表达的钙激活钾(KCa)通道进行种类鉴别和特征研究。结果表明:在高对称钾溶液(200mmol/L)中,只有小颗粒细胞表达大电导钙激活钾(BKCa)通道和中电导钙激活钾(IKCa)通道。其中BKCa通道电导为396pS,通道的开放活动表现为矩形方波。同一钳制电压下,当浴液中[Ca~(2+)]在0.3～3.0 μmol/L范围内,通道开放概率和开放数目随Ca~(2+)浓度增加而增加,当浴液中[Ca~(2+)]达到100.0 μmol/L,通道开放受到抑制,表现明显的钙依赖性失活;当浴液中Ca~(2+)浓度为3.0 μmol/L,钳制电压分别为-40mV和-80mV时,通道平均开放时间分别为(0.03233±0.00741)s和(0.05585±0.01299)s(P0.01),平均开放概率分别为0.596±0.125和0.961±0.012(P0.01),说明通道开放具有电压依赖性。IKCa通道电导为88pS,开放活动也表现为矩形方波。当浴液中Ca~(2+)浓度为0.3、1.0μmol/L时,通道不开放;而Ca2+浓度为3.0μmol/L时通道开放,说明通道具有明显的钙依赖性,钳制电压分别为-40mV和-80mV时,通道平均开放时间分别为(0.01736±0.00403)s和(0.02262±0.01616)s(P0.05),平均开放概率分别为0.699±0.065和0.790±0.114(P0.05),说明通道开放不具有明显的电压依赖性。药理学研究结果表明,40mmol/L四乙铵(TEA)灌流15min能完全阻断KCa通道活动。这提示钙激活钾通道在甲壳动物细胞免疫中发挥重要作用。  相似文献   

Bioaccumulation of polychlorinated biphenyls in prawns and a mud crab from mixed-aquaculture ponds     

JIANG Jinhua 《海洋学报(英文版)》2009,28(6):111-117

The bioaccumulation of ten polychlorinated biphenyl (PCB) congeners was investigated within different tissues in three prawns (Penaeusorientalis Kishinouce, Laomedia astacina de Haan and Penaeus vannamei Boone) and one mud crab (Scylla serrata Forsskal) from mixed-aquaculture ponds in Taizhou in April 2007. The average concentrations (wet mass) of Σ PCBs in muscle, head, and rind tissues for mean among these prawn species were 7.04, 21.16, and 11.47 ng/g, respectively. The average concentrations of Σ PCBs in muscle, branchia, and spawn in Scylla serrata were 6.49, 21.13, and 19.76 ng/g, respectively. The concentrations of CB28 were the highest in prawn tissues (2.40 ng/g), and accounted for 18.60% of Σ PCBs. The concentration of CB52 was the lowest. The application of principal component analysis (PCA) for the estimation of PCB sources of contamination resulted in first component main contributions of CB101, CB153 and CB28 in the muscle, head, and rind in combined prawn samples, respectively. The enrichment level of Σ PCBs for different tissues varied greatly. The accumulation coefficient of Σ PCBs in prawn muscle, head, and rind tissues were 396.35, 1 191.79, and 645.91, respectively. For the Scylla serrata, the accumulation coefficients were 365.81, 1 190.50, and 1 113.31 for muscle, branchia, and spawn tissues, respectively. For prawns, the accumulation coefficient of CB153 was the highest in muscle tissues, while CB155 had the highest coefficients in the head and rind tissues. The CB155 coefficient was the highest in spawn tissues of the mud crab.  相似文献   

南北水系中华绒螯蟹形态差异分析   总被引：4，自引：0，他引：4  

何杰  徐跑  朱健 《海洋湖沼通报》2009,(3)

为探讨南北水系中华绒螯蟹形态差异及物种有效性问题,采用形态学测量的方法,每只蟹选择56个测量点,测量南北水系蟹各60只的54个形态性状.主成分分析显示,南北样本可以分成两个相互独立的群体.t检验显示,54个所测量形态变量中有25个南北差异极其显著(P<0.01),5个差异显著(0.01相似文献   

短小拟五角蟹的分布新记录          下载免费PDF全文

宁璇璇  韩庆喜 《海洋科学》2023,47(6):91-95

2022年9月作者在浙江宁波象山港海域采集到短小拟五角蟹(Paranursia abbreviata),由此短小拟五角蟹的地理分布记录从中国的海南岛、广东、香港和福建扩展到东海北部,即浙江北部的象山港。本文对其形态特征进行了详细的描述,并讨论了其与历史记载的外形差异。由于象山港冬季水温较低,推测短小拟五角蟹在象山港的出现可能是台湾暖流携带幼体定居导致的偶然现象。  相似文献