中国沿海脉红螺(Rapana venosa)自然群体线粒体 DNA 16S rRNA遗传特性研究   总被引：7，自引：1，他引：6  

杨建敏  李琪  郑小东  宋志乐  王如才 《海洋与湖沼》2008,39(3):257-262

对中国沿海脉红螺7个自然居群的线粒体DNA 16S rRNA基因片段进行了扩增和测序,分析了107个个体511bp的碱基序列,结果没有发现插入,缺失突变的核苷酸位点.检测到了28个多态性核苷酸位点,共37种单倍型,单倍型1的同源性达到58.7%.大连居群的28l(G-A)和483(T- C)位点,烟台居群的17(A-G)位点、66(T- C)和247(T-C)位点,舟山居群的128(T-C)位点,秦皇岛居群的130(T-G)和161(A-G)位点,以及丹东居群的431(T-C)位点的变异可以作为居群分子遗传标记位点.居群内,烟台和大连居群的核苷酸差异数 K 以及平均核苷酸多样性指数 Pi 最高,青岛居群最低;居群间,大连居群在居群间核苷酸差异数K、居群间平均每位点核苷酸替代数Dxy 和居群间每位点净核苷酸替代数Da三个指标上都表现出比其他居群之间较高的水平,说明大连居群具有最为丰富的遗传多样性.  相似文献   

Colonization of Sulfurovum sp. on the gill surfaces of Alvinocaris longirostris, a deep-sea hydrothermal vent shrimp     

Gaku Tokuda  Akinori Yamada  Kazuma Nakano  Nao O. Arita  & Hideo Yamasaki 《Marine Ecology》2008,29(1):106-114

Deep‐sea hydrothermal vents are unique light‐independent ecosystems that are sustained by chemosynthetic bacteria. For many of the invertebrates inhabiting in such environments, bacteria play essential roles in both energy acquisition and detoxification of potentially toxic gases such as H₂S. In this study, the bacterial flora present on the gills of Alvinocaris longirostris (Bresiliidae: Caridea), a shrimp inhabiting hydrothermal vents (1532 m depth) at the Hatoma Knoll of the Okinawa Trough, was investigated. Bacterial 16S rDNA fragments were successfully amplified from the gills and 70% of these fragments showed an identical pattern in the restriction fragment length polymorphism analysis. These fragments were assigned to the ribotype AL‐1. Phylogenetic analyses suggest that AL‐1 forms a monophyletic clade with Sulfurovum spp. (ε‐Proteobacteria). Fluorescence in situ hybridization for AL‐1 and electron microscopy showed the presence of short‐rod bacteria lining up on the cuticular layer of the surface of the gill filaments. These results suggest that bacterial association with gills also occurs in bresiliid shrimps.  相似文献   

海参、海胆养殖水体中多抗性细菌抗性基因的初步研究     

陈明娜  党宏月 《海洋科学》2008,32(3):13-18

抗药性细菌对生态环境、养殖业以及人类健康构成严重威胁。以大连海参、海胆养殖场多抗性细菌筛选为基础,采用分子生物学手段对40株抗性细菌进行了氯霉素、土霉素及氟氯霉素抗性基因检测,探讨抗药性分子遗传机理,从而为控制抗药性细菌的产生和传播提供研究基础。  相似文献   

Evidence for population structuring of blue whiting (Micromesistius poutassou) in the Northeast Atlantic     

Was  Anna; Gosling  Elizabeth; McCrann  Karen; Mork  Jarle 《ICES Journal of Marine Science》2008,65(2):216-225

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Cloning and expression of prion protein encoding gene of flounder （Paralichthys olivaceus）     

张之文  孙修勤  张进兴  昝金东 《中国海洋湖沼学报》2008,26(1):50-53

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Cloning and expression analysis of a prion protein encoding gene in guppy (Poecilia reticulata)     

Suihan Wu  Qiwei Wei  Guanpin Yang  Dengqiang Wang  Guiwei Zou  Daqing Chen 《中国海洋大学学报(英文版)》2008,7(4):425-431

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Cloning and Expression Analysis of a Prion Protein Encoding Gene in Guppy （Poecilia reticulata）     

WU Suihan  WEI Qiwei  YANG Guanpin  WANG Dengqiang  ZOU Guiwei  CHEN Daqing 《中国海洋大学学报(英文版)》2008,7(4)

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一株海水氨氧化细菌的系统发育分析     

黄建军  简纪常  吴灶和  丁燏 《广东海洋大学学报》2008,28(3)

应用PCR技术扩增16S rRNA基因和amoA(ammonia monooxygenase subunit A)的基因片段,并测定其序列,对一株源于海水养殖水体的高效氨氧化细菌(ammonia-oxidizing bacteria,AOB)进行了系统发育分析。结果表明,经PCR扩增得到了1 098 bp的16S rRNA基因片段和491 bp的amoA基因片段,将其序列用NCBI-Blast软件在GenBank数据库中进行同源性检索后发现,该菌株的16S rRNA基因序列和amoA基因序列分别与亚硝化单胞菌Nitrosomonas sp.NS20的相对应基因片段相似性分别为98.4%和96.7%。在此基础上构建了系统发育树,表明用该菌株的16S rRNA基因片段和amoA基因片段构建的系统发育树均与亚硝化单胞菌属类聚在一起,结合该菌株形态和生理生化特性,鉴定该株氨氧化细菌属亚硝化单胞菌。  相似文献   

Cloning and analysis of phycoerythrin genes inGracilaria Lemaneiformis (Rhodophyceae)     

Sui Zheng-hong  Zhang Xue-cheng 《中国海洋湖沼学报》2000,18(1):42-46

Cloning and sequencing of the genes coding for the α- and β- subunit of phycoerythrin (PE) of a red alga—Gracilaria lemaneiformis (GL) are reported. Alignment of 1084 nucleotides sequenced with three known red algal PE genes,Rhodella violacea (RV),Polysiphonia boldii (PB) andAglaothamnion neglectum (AN), showed high level of conservation, and similarities of 77,6% (between GL and RV), 77.9% (GL and AN) and 79.0% (GL and PB). The similarities of amino acids were 84.8% (between GL and RV), 85.7% (GL and PB), and 80.6% (AN and GL), higher than those among nucleotides. Project 39670405 supported by NSFC and Japanese Science Promotion Society (JSPS).  相似文献   

关于实施贯标认证的几点思考     

肖中文 《地理信息世界》2000,(1)

按照ＧＢ／Ｔ１９０００－ＩＳＯ９０００系列标准的基本要求,建立、完善测绘质量体系的工作已在测绘业全面展开,这对子提高测绘职工的素质和技能,对测绘行业现代化的生产管理将产生积极的影响。  相似文献