南海三沙永乐龙洞古菌群落结构与多样性特征     

甄毓  贺惠  傅亮  刘乾  毕乃双  杨作升 《海洋与湖沼》2018,49(6):1259-1267

海洋蓝洞作为一种独特的海洋地貌单元,洞内的生物群落结构与生态系统特征一直是国内外同行学者关注的热点问题之一。南海三沙永乐龙洞是世界上最深的海洋蓝洞(300m),其独特的生态特征更为引入瞩目。本研究以古菌16S rRNA基因序列为目标,采用Illumina高通量测序技术对西沙永乐龙洞内水体与沉积物中的古菌群落结构进行了研究。结果表明,本研究所获得的古菌类群可归为4门、21纲、29目、42科、45属,广古菌和奇古菌是丰度最高的两种古菌。龙洞内表层水体中古菌群落多样性与洞外水体相差不大,但随深度增加,洞内古菌群落多样性显著降低,深水层古菌群落结构与浅水层差异显著。龙洞内沉积物中的古菌生存环境与水体差异巨大,为大量特殊物种提供了生存空间。侧壁沉积物中的古菌群落多样性随深度增加显著降低,而150m平台处古菌群落多样性则远高于侧壁沉积物。水温对龙洞内水体中的古菌群落结构具有显著影响(P0.01)。研究结果显示永乐龙洞古菌群落有很高的多样性,其垂向分布与洞内环境多要素的变化密切相关。本研究对认识三沙永乐龙洞内的生态系统特征有显著意义。  相似文献   

Isolation and diversity analysis of heterotrophic bacteria associated with sea anemones   总被引：2，自引：0，他引：2  

DU Zongjun  ZHANG Wanyi  XIA Hongjie  L&#; Guoqiang  CHEN Guanjun 《海洋学报(英文版)》2010,29(2):62-69

A study was undertaken to investigate the heterotrophic bacterial flora associated with the sea anemones. Samples of the sea anemones Anthopleura midori were collected from the coast of Weihai and bacteria were isolated from these samples. Additionally, high numbers of viable bacteria were obtained from the celom wall and surface of anemone, the community of cultivable bacteria was very diverse. As a result of this isolation, 60 strains were obtained, 56 of them were selected for identification and characterization by 16S rRNA gene sequence analysis and limited phenotypic testing. Among these isolates, 16 strains were phylogenetically related to members of the genus Pseudoalteromonas and neighboring taxa. Other isolates included members of the genera Colwellia, Vibrio, Acinetobacter, Pseudomonas, Endozoicomonas, Roseovarius, Paracoccus, Loktanella, Leisingera, Sulfitobacter, Bacillus, Staphylococcus, Plantibacter, Microbacterium, Micrococcus, Joostella, Psychroserpens, Cellulophaga, Krokinobacter, Polaribacter and Psychrobacter. Seven potential novel species were found. Among 60 strains, 17 of them can produce proteolytic exoenzyme, 20 can produce lipolytic exoenzyme. Strain NQ8 has strong antagonistic effects on some Vibrio strains. This study demonstrates that the culturable fraction of bacteria from the sea anemones Anthopleura midori is diverse and appears to possess much potential as a source for the discovery of novel bioactive materials.  相似文献   

一株海水氨氧化细菌的系统发育分析     

黄建军  简纪常  吴灶和  丁燏 《广东海洋大学学报》2008,28(3)

应用PCR技术扩增16S rRNA基因和amoA(ammonia monooxygenase subunit A)的基因片段,并测定其序列,对一株源于海水养殖水体的高效氨氧化细菌(ammonia-oxidizing bacteria,AOB)进行了系统发育分析。结果表明,经PCR扩增得到了1 098 bp的16S rRNA基因片段和491 bp的amoA基因片段,将其序列用NCBI-Blast软件在GenBank数据库中进行同源性检索后发现,该菌株的16S rRNA基因序列和amoA基因序列分别与亚硝化单胞菌Nitrosomonas sp.NS20的相对应基因片段相似性分别为98.4%和96.7%。在此基础上构建了系统发育树,表明用该菌株的16S rRNA基因片段和amoA基因片段构建的系统发育树均与亚硝化单胞菌属类聚在一起,结合该菌株形态和生理生化特性,鉴定该株氨氧化细菌属亚硝化单胞菌。  相似文献   

中国沿海脉红螺(Rapana venosa)自然群体线粒体 DNA 16S rRNA遗传特性研究   总被引：7，自引：1，他引：6       下载免费PDF全文

杨建敏  李琪  郑小东  宋志乐  王如才 《海洋与湖沼》2008,39(3):257-262

对中国沿海脉红螺7个自然居群的线粒体DNA 16S rRNA基因片段进行了扩增和测序,分析了107个个体511bp的碱基序列,结果没有发现插入,缺失突变的核苷酸位点.检测到了28个多态性核苷酸位点,共37种单倍型,单倍型1的同源性达到58.7%.大连居群的28l(G-A)和483(T- C)位点,烟台居群的17(A-G)位点、66(T- C)和247(T-C)位点,舟山居群的128(T-C)位点,秦皇岛居群的130(T-G)和161(A-G)位点,以及丹东居群的431(T-C)位点的变异可以作为居群分子遗传标记位点.居群内,烟台和大连居群的核苷酸差异数 K 以及平均核苷酸多样性指数 Pi 最高,青岛居群最低;居群间,大连居群在居群间核苷酸差异数K、居群间平均每位点核苷酸替代数Dxy 和居群间每位点净核苷酸替代数Da三个指标上都表现出比其他居群之间较高的水平,说明大连居群具有最为丰富的遗传多样性.  相似文献   

基于MOD16的山西省地表蒸散发时空变化特征分析   总被引：1，自引：1，他引：1  

温媛媛  赵军  王炎强  王玉纯  王建邦 《地理科学进展》2020,39(2):255-264

基于MOD16全球蒸散发产品和气象站点实测数据,运用变异系数法、Sen趋势法等研究了山西省2000—2014年地表蒸散发ET、潜在蒸散发PET的空间分布特征、变化趋势及影响因素。结果表明：① MOD16蒸散产品与气象站点实测蒸散发之间具有良好的时空相关性(R ²=0.90),其产品精度可以满足山西省蒸散发时空分布研究的要求;②山西省多年平均ET、PET分别为816.77、1608.46 mm,年内变化表现为先增高后下降的“单峰”型分布,二者差值在5月、6月最大,此时山西省最为干旱;③ 全省年平均ET呈现西北低、东南高的分布特征,PET呈西南高、东北低的分布特征,二者差值整体上较大,表现为全省地表水分比较缺乏,其中忻州、吕梁西部最为严重;④ 全省近15 a来ET和PET的年际变化都比较小,整体上全省PET在增加,ET在相对减少,意味着近15 a来干旱情况在加剧;⑤ ET、PET的时空变化与诸多气象因子相关,在空间尺度上与降水、相对湿度密切相关,在时间尺度上与气温、降水关系最为密切。  相似文献   

Periodic nulls in the pulsar B1133+16     

Jeffrey L. Herfindal  Joanna M. Rankin 《Monthly notices of the Royal Astronomical Society》2007,380(2):430-436

Numerous studies of the brightest Cambridge pulsar, B1133+16, have revealed little order in its individual pulses, apart from a weak 30-odd-rotation-period fluctuation feature and that some 15 per cent of the star's pulsars are 'nulls'. New Arecibo observations confirm this fluctuation feature and that it modulates all the emission, not simply the 'saddle' region. By replacing each pulse with a scaled version of the average profile, we were able to quench all subpulse modulation and thereby demonstrate that the star's 'null' pulses exhibit a similar periodicity. A subbeam carousel model with a sparse and irregular 'beamlet' population appears to be compatible with these characteristics.  相似文献   

东海陆架表层沉积物细菌群落结构及地理分布研究     

刘明华  王健鑫  俞凯成  蒋然  刘晓辉  王帅兵  刘雪珠 《海洋与湖沼》2015,46(5):1119-1131

本研究通过构建16S r DNA克隆文库,对东海陆架五个站点的沉积物样品进行群落结构及地理分布研究。结果表明:东海陆架沉积物细菌分属于13个类群,包括变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、酸杆菌门(Acidobacteria)、绿弯菌门(Chloroflexi)、放线菌门(Actinobacteria)、硝化螺旋菌门(Nitrospira)、WS3、拟杆菌门(Bacteroidetes)、螺旋体门(Spirochaetes)、OP8、浮霉菌门(Planctomycetacia)、疣微菌门(Verrucomicrobiae)和芽单胞菌门(Gemmatimonadetes),其中变形菌门(γ-变形菌纲、δ-变形菌纲)和绿弯菌门为优势菌群,Latescibacteria(WS3)、疣微菌门和芽单胞菌门分别是站点DH6、DH21和DH9的特有类群。五个站点细菌多样性从高到低排序依次为DH9DH21DH17DH6DH16,其群落结构和丰度与各站点不同沉积环境类型有关。  相似文献   

椒江口化工园区邻近海域沉积物中细菌多样性初步研究     

蒋然  王健鑫  黄备  张潘  郑俊威  俞凯成  刘明华 《海洋与湖沼》2015,46(6):1420-1437

为研究椒江化工园区邻近海域沉积物中细菌结构, 选取四个典型站点, 通过分离纯化、鉴 定和建立克隆文库的方法, 对其进行多样性及系统发育分析。可培养细菌的形态学及API 鉴定结果 显示洋葱假单胞菌及杀鲑气单胞菌杀鲑亚种是优势种, 典型细菌16S rDNA 分子鉴定结果表明厚壁 菌门及γ-变形菌纲为主要类群。非培养细菌克隆文库的序列分析结果表明: 细菌主要包括变形菌门、 酸杆菌门、绿弯菌门、芽单胞菌门、硝化螺旋菌门、CFB 群、放线菌门、厚壁菌门、浮霉菌门等9 个类群; 其中C3 站点克隆子主要属于γ-变形菌纲及δ-变形菌纲; C4 站点克隆子以γ-变形菌纲及酸杆 菌门为主; C5 站点克隆子主要属于δ-变形菌纲及γ-变形菌纲; C6 站点克隆子主要属于γ-变形菌纲及 放线菌门。综合可培养及非培养结果, 可发现椒江口化工园区邻近海域沉积物中γ-变形菌纲为典型 优势类群, 除相当数量的克隆子相似序列来自石油烃污染的沉积环境外, 还有大量克隆子相似序列 来自养殖污染环境。  相似文献   

内蒙古河套灌区湿地芦苇根际可培养细菌多样性     

孙磊  刘莹  姚娜  邵红  李潞滨 《湿地科学》2012,10(2):176-180

以内蒙古河套灌区湿地植物芦苇(Phragmites australis)根际土壤为材料,采用分离培养法对天然湿地和退化湿地的芦苇根际细菌多样性进行了研究。16SrRNA基因系统发育分析的结果显示,分离自天然湿地芦苇根际的45株细菌分属于γ-变形菌纲(44.44%)、厚壁菌门(Firmicutes)(42.22%)和放线菌门(Actinobacteria)(13.33%)3大类群,其中,γ-变形菌纲为最优势类群,芽孢杆菌属(Bacillus)(42.2%)为最优势菌属;分离自退化湿地芦苇根际的44株细菌分属γ-变形菌纲(13.63%)、β-变形菌纲(6.82%)、厚壁菌门(47.73%)及放线菌门(Actino-bacteria)(31.82%)4大类群,其中厚壁菌门为最优势类群,芽孢杆菌属(40.9%)为最优势菌属。研究结果表明,天然湿地和退化湿地芦苇根际细菌都具有较丰富的多样性,但天然湿地与退化湿地芦苇根际细菌群落结构存在一定差异。  相似文献   

Eight new and three recalculated orbits for binaries     

Z. Cvetkovi&#x;  B. Novakovi&#x; 《Astronomische Nachrichten》2010,331(3):304-311

In this paper new orbital elements are given for eleven binaries. For eight of them, WDS 00003–4417 = I 1477, WDS 00106–7313 = I 43 AB, WDS 00366+5609 = A 914, WDS 00519–4343 = I 47, WDS 01315+1521 = BU 506, WDS 01577+4434 = A 1526, WDS 08144–4550 = FIN 113 AB and WDS 08291–4756 = FIN 315 Aa‐Ab, the orbital elements are calculated for the first time. For three of them, WDS 04422+2257 = MCA 16 Aa‐Ab, WDS 08275–5501 = FIN 116 and WDS 14567–6247 = FIN 372, the orbital elements are recalculated. One of the eleven binaries, MCA 16 Aa‐Ab, was discovered by McAlister in 1980 by speckle interferometry and four pairs were discovered by Finsen between 1929 and 1960. For these five pairs, all measured separations are less than 0″.4 and most of the observations were done by using the interferometric techniques. The orbital periods calculated here are between 39 and 270 years. The remaining six pairs were discovered between 1878 and 1926 and most of the observations are visual. They have longer orbital periods, between 384 and 1637 years. In addition to the orbital elements the masses, dynamical parallaxes, absolute magnitudes and ephemerides for the next five years are also given in this paper (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)  相似文献