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11.
【目的】通过转录组测序分析雌激素(17β-Estradiol,E2)对雌性金钱鱼下丘脑中基因表达的影响。【方法】E2体注射后进行雌性金钱鱼下丘脑转录组测序,基因功能注释,差异基因筛选、鉴定,GO(Gene Ontology annotation)和KEGG(Kyoto Encyclopedia of Genes and Genomes)信号通路富集分析,并利用实时定量PCR(Real-time quantitative PCR,qPCR)检测E2体注射后雌性金钱鱼下丘脑中相关基因的表达。【结果】雌性金钱鱼下丘脑转录组测序共测得Clean reads为190909756,注释到17687个基因,筛选和鉴定了22个差异表达基因,包括prl、erf3α、pgr和cyp19a1b等11个表达上调基因和chsy1、muc17l、prf1和hla-α等11个下调基因。通过KEGG功能富集分析发现差异基因涉及13个代谢通路。转录组和qPCR结果显示,与对照雌鱼相比,E2注射组雌鱼下丘脑中prl、pgr、cyp19a1b和3β-HSDI的表达显著上调,而生长轴相关基因ghrh、sst1、sst3、sst5和sst6的表达未受影响。【结论】E2通过反馈作用调节下丘脑中部分性类固醇激素合成通路相关基因的表达,但不影响生长相关基因的表达。 相似文献
12.
The haematological, biochemical, and enzymological alterations produced on exposure of Channa punctatus to LC50 (11.2 mg/L) for 96 h and to a sublethal concentration (1.12 mg/L) of cadmium (Cd2+) for 15, 30, 60, and 120 days have been studied. The fish were hypoglycemic and hypolactemic. The pyruvate content of blood and liver decreased in acute and all stages of chronic exposure except for 30 days where significant increase was recorded. Depletion was noted in the total protein and glycogen content of liver and muscle, and the level of lactate in liver in the two types of exposure. The levels of muscle lactate and pyruvate increased in both exposures. The activities of hexokinase, glucose-6-phosphatase, and lactate dehydrogenase decreased in liver after 96 h and all stages of chronic exposure except 30 days. The activities of hexokinase and lactate dehydrogenase in muscle and glutamate-oxalacetate transaminase and glutamate-pyruvate transaminase in serum, liver, and muscles increased in all exposures. The activities of succinate and malate dehydrogenases in liver and muscles decreased after acute and all stages of chronic exposure. The above results suggest that glycolysis and gluconeogenesis were impaired in liver but glycolysis prevailed in muscle. Aerobic oxidation is adversely affected in metal exposed fish, while proteolysis was increased. 相似文献
13.
盐度对乌鳢(Channa argus)生长和肌肉品质的影响 总被引：2，自引：0，他引：2
设置盐度为0(对照组)、5.0、7.5、10.0的4个水体,饲养平均体重为(402.4±44.6)g的乌鳢30天,测定乌鳢生长性能、肌肉成分、游离氨基酸和肌肉物理性能指标,研究盐度对乌鳢生长和肌肉品质的影响。结果表明,各组乌鳢的增重率分别为37.7%、33.9%、31.5%、6.3%,成活率分别为100%、100%、100%和63.6%,表现出随盐度增加而下降的趋势,但盐度0、5.0、7.5各组间无显著差异,在盐度为10.0的水体中,乌鳢成活率和增重率均显著降低(P<0.05),饲料系数显著增加(P<0.05);随盐度增加,肌肉水分和肌肉失水率增加,而脂肪含量减少;肌肉蛋白含量和胶原蛋白含量在盐度0、5.0、7.5各组间无差异,但在盐度10组显著降低;与对照组相比,盐度7.5组的肌原纤维耐折力增加,肌纤维直径减小;随盐度升高,肌肉质构指标(硬度、弹性、黏性、咀嚼性、凝聚性)均有不同程度改善;在肌肉游离氨基酸方面,随盐度增加,肌肉游离呈味氨基酸和总游离氨基酸含量呈上升趋势,其中盐度10.0组较对照组分别增加88.2%、42.7%(P<0.05)。上述研究表明,适宜的盐度可改善乌鳢的肌肉品质,结合鱼体生长性能,适宜的盐度为7.5。 相似文献
14.
B. F. Phillips Y. W. Cheng C. Cox J. Hunt N. K. Jue R. Melville‐Smith 《新西兰海洋与淡水研究杂志》2013,47(3):715-722
This study compared catches of Panulirus argus pueruli and very young juveniles between Hunt and Sandwich collectors at Long and Big Munson Keys, Florida, United States. Catch comparisons were made over the peak puerulus settlement period between February and July 2002, using six Sandwich and six Hunt collectors at each site. The collectors were set out in arrays and the relative positions of the two collector types were reversed each month after they had been checked and the catch removed. A total of 3470 pueruli and juveniles were collected; 2011 and 1459 by Sandwich and Hunt collectors, respectively. Sandwich collectors caught an average of 5.43 more pueruli and juveniles per check than Hunt collectors, with this difference being significant for catches of clear pueruli (P1s), pigmented pueruli (P3s), and juvenile stages (J1s and J2s) (P < 0.05), but not for catches of semi‐pigmented pueruli (P2s). The catches made by both collector types were highly dependant on the month of collection and site of the collectors (P < 0.01). Catches were generally not influenced by the location of the collector in the array (P = 0.50), but corner collectors caught significantly fewer P1s, P2s, P3s, and J1s and J2s than all other collectors (P < 0.01). Conditioning time influenced the total catch (P < 0.01) but was not significant for all individual P1s, P2s, P3s, and J1s and J2s stages of development. Overall, the time taken for collectors to condition in Florida was less than the Sandwich collectors used for P. cygnus in Western Australia. 相似文献
15.
凡纳滨对虾与金钱鱼混养模式水质理化因子及浮游生物群落结构的变化 总被引：1，自引：0，他引：1
以凡纳滨对虾(Litopenaeus vannamei)与金钱鱼(Scatophagus argus)混养池塘和凡纳滨对虾单养池塘为研究对象,对两池塘水体的理化因子及浮游生物进行测定。结果显示:混养池塘水体的溶解氧与单养池塘相比均无显著性差异;混养和单养池塘的化学需氧量(COD)、亚硝酸盐和氨氮的含量在实验过程中均呈现持续上升趋势,但混养池上升趋势较缓,两池塘的上述指标在养殖前期差异不显著,养殖后期有显著差异(P<0.05)。在养殖后期,混养池塘的浮游植物丰度显著大于单养池塘(P<0.05),而浮游动物丰度显著低于单养池塘(P<0.05)。结果表明,混养金钱鱼可有效改善凡纳滨对虾精养池塘的水质,降低亚硝酸盐、氨氮及COD的含量,稳定浮游植物种群结构,控制池塘中浮游动物的过度繁殖。 相似文献
16.
鳃是鱼类渗透压调节的主要器官, 鳃细胞的培养技术的建立可以为鱼类渗透压调节机理研究提供重要的实验手段和研究方法。金钱鱼(Scatophagus argus)作为一种广盐性鱼类, 是研究渗透压调节机制的理想动物模型。本研究采用胰蛋白酶消化法进行了金钱鱼鳃细胞的体外培养, 确定该类细胞原代和传代培养的最优条件, 分析了其生长特性。结果表明, 金钱鱼鳃细胞系在28℃, 含有20%胎牛血清(fetal bovine serum, FBS)的L-15培养基中生长最佳, 2~4d即可传代, 传代后可稳定培养, 命名为SG。在传代培养过程中, 对其增殖情况进行分析, 发现SG细胞群体倍增时间为40.8h。正常传代的SG细胞冻存、复苏后, 经台盼蓝染色测得细胞存活率约为87%。鳃细胞直接同水体环境接触, 具有高效的渗透压调节能力。环境盐度变化时, 鳃细胞启动渗透压胁迫应答机制, 维持内环境稳态。本研究对SG细胞进行渗透压刺激后, 检测其增殖情况并观察形态变化。分析表明, SG细胞在分别为150和600mOsmol·kg -1的低渗和高渗胁迫后均可增殖, 且低渗胁迫后的增殖速度是高渗胁迫的1.5倍。渗透压胁迫后SG细胞的形态观察结果显示, 低渗培养基胁迫后细胞体积发生膨胀而高渗条件下细胞体积发生皱缩。由此推断SG细胞具有较强渗透压耐受性, 且低渗耐受能力强于高渗。SG细胞系的建立为金钱鱼渗透压应答机制和相关基因功能的研究提供了基础实验材料。 相似文献
17.
采用RT-PCR和3′RACE技术,首次从乌鳢胃组织中克隆了一种胃蛋白酶原基因。测序结果表明,该基因开放阅读框全长1086bp编码361个氨基酸,递交GeneBank数据库,获得登录号为GQ303143。系统进化关系分析表明,该乌鳢胃蛋白酶原氨基酸序列与鳜鱼类的胃蛋白酶原A2亲缘关系最近,从而推测本研究克隆的乌鳢胃蛋白酶原属于胃蛋白酶原A2类。采用生物信息学的方法和工具预测了该乌鳢胃蛋白酶原的理化参数及其高级结构,结果表明,该乌鳢胃蛋白酶原的相对分子量为38.8kDa,理论等电点为4.56。采用同源建模法建立了乌鳢胃蛋白酶原的三维结构模型,预测了其活性位点及6个必需半胱氨酸残基的位置。该研究结果为进一步研究乌鳢胃蛋白酶的结构和功能关系奠定了基础。 相似文献
18.
采用垂直板聚丙烯酰胺凝胶电泳技术,对月鳢十种组织中乳酸脱氢酶（ＬＤＨ）和酯酶（ＥＳＴ）同工酶进行了比较研究,结果表明：月鳢组织内ＬＤＨ和ＥＳＴ具有明显的组织特异性,ＥＳＴ由２个座位编码,ＬＤＨ则由Ｌｄｈ－ａ、Ｌｄｈ－ｂ两个基因编码。 相似文献
19.
This experiment on the effects of fishes (Pelteobagrus fulvidraco, Channa argus, Cyprinus carpio) on growth and survival of juvenile Chinese mitten crab,Eriocheir sinensis was conducted in cages placed in Bao'an Lake. T-tests showed that effects of fishes on the carapace width growth were not
significant as compared with the control (no fish). But the effects of these fishes on crab survival were negative. The causes
of negative effects of fishes on crab survival were simply discussed on the basis of food habits.
This research was financially supported by the Department of Agriculture (96-008-02-03), P. R. China, and Chinese Academy
of Sciences (Key Project: KZ951-A1-102). 相似文献
20.
季节性降雨和洋流运动常常使沿岸海水盐度呈现波动状态。环境盐度的改变往往导致鱼类更易受到病原的侵害而患病死亡。因此, 探究盐度对金钱鱼(Sscatophagus argus)免疫功能的影响在渔业养殖业具有相当重要的参考价值。本文通过比较各盐度组金钱鱼经嗜水气单胞菌(Aeromonas hydrophila)感染后96h(96hpi)内血清和肾脏抗氧化酶(superoxide dismutase, SOD)浓度以及免疫相关参数Complement 4(C4)、Interleukin-6(IL-6)和Immunoglobulin M(IgM)的变化, 来分析盐度的改变对其血清和肾脏免疫功能的影响。结果显示, 除96hpi外, 血液和肾脏组织中淡水组和低盐组中金钱鱼体内SOD浓度总体均比25‰盐度组高, 且最大差异可达150ng·mL-1左右, 其中血清结果差异极显著(P<0.01); 淡水组与低盐组中金钱鱼经嗜水气单胞菌感染后96h内血清和肾脏组织C4浓度总体均比25‰盐度组高100~600μg·mL-1 (P<0.01)左右; 虽然三个盐度感染嗜水气单胞菌后组肾脏组织IL-6浓度(14.2±0.1、17.9±0.0和17.9±0.0pg·mL-1) 显著低于对照组(P<0.01), 25‰盐度组肾脏组织IL-6水平仍显著高于淡水组和低盐组(P<0.01), 而淡水组的血清IL-6浓度在96hpi内显著增加至56.9±1.0pg·mL-1 (P<0.01); 25‰盐度组金钱鱼血清和肾脏组织IgM浓度(71.8±2.9和6.3±0.4μg·mL-1)比淡水组和低盐组高约1~20μg·mL-1, 且差异显著(P<0.05)。同时, 25‰盐度组血清和肾脏组织大量产生抗体IgM时间(12hpi)早于淡水组和低盐组(24hpi)。综上所述, 25‰盐度组在细菌感染后受到的损伤较小, 肾脏及血清免疫状态优于淡水组与低盐组, 且免疫应答反应更迅速。因此推测, 盐度的降低将会导致金钱鱼血清和肾脏免疫状态下降。本研究可为调节金钱鱼的养殖盐度提供参考。 相似文献