Genetic mapping and QTL analysis for body weight in Jian carp (Cyprinus carpio var. Jian) compared with mirror carp (Cyprinus carpio L.)     

顾颖  鲁翠云  张晓峰  李超  俞菊华  孙效文 《中国海洋湖沼学报》2015,33(3):636-649

We report the genetic linkage map of Jian carp(C yprinus carpio var. Jian). An F1 population comprising 94 Jian carp individuals was mapped using 254 microsatellite markers. The genetic map spanned 1 381.592 c M and comprised 44 linkage groups,with an average marker distance of 6.58 c M. We identified eight quantitative trait loci(QTLs) for body weight(BW) in seven linkage groups,explaining 12.6% to 17.3% of the phenotypic variance. Comparative mapping was performed between Jian carp and mirror carp( Cyprinus carpio L.),which both have 50 chromosomes. One hundred and ninety-eight Jian carp marker loci were found in common with the mirror carp map,with 186(93.94%) showing synteny. All 44 Jian carp linkage groups could be one-to-one aligned to the 44 mirror carp linkage groups,mostly sharing two or more common loci. Three QTLs for BW in Jian carp were conserved in mirror carp. QTL comparison suggested that the QTL confidence interval in mirror carp was more precise than the homologous interval in Jian carp,which was contained within the QTL interval in Jian carp. The syntenic relationship and consensus QTLs between the two varieties provide a foundation for genomic research and genetic breeding in common carp.  相似文献   

微型生态系统中鲢、鳙下行影响的实验研究——2.营养物水平   总被引：4，自引：0，他引：4  

阮景荣  戎克文  王少梅 《湖泊科学》1995,(4)

鲢、鳙放养使微型生态系统的水柱氮、磷浓度和磷的分布发生了明显的变化。至实验结束时,各实验组的水柱颗粒磷、总磷和氨氮浓度都比对照组高,而正磷酸盐浓度和沉积物磷的量均低于对照组。这种变化以鳙单养系统为最大,其次是鲢、鳙混养系统,鲢单养系统的变化最小。微型生态系统中正磷酸盐浓度同浮游动、植物密度和初级生产力显著相关,氨氮浓度同所述变量之间的相关关系则多半与正磷酸盐相反。实验观测期间浮游植物密度与总磷浓度之间存在营养级联假说所预见的下行影响,实验结束时二者之间却有上行影响的趋向。根据实验观测结果认为,微型生态系统营养物(尤其是磷)水平的变动,主要是鲢、鳙的摄食改变了系统的群属结构和代谢强度的结果,同时反映了实验鱼对系统中营养物再生的影响。  相似文献   

共炮检距二维弹性波地震剖面的偏移方法   总被引：1，自引：0，他引：1  

马在田 《地球物理学报》1995,38(Z1):1-10

在均匀各向同性介质的分界面上入射纵波时，除产生纵波反射Ｐ－Ｐ波外，还会产生转换横波的反射Ｐ－ＳＶ波。同样，当入射波为ＳＶ横波时，在分界面上会同时产生ＳＶ－ＳＶ反射波和ＳＶ－Ｐ反射转换波，对这种二维弹性波地震记录如何进行偏移是正在探讨的问题。为了能够处理实际地震数据，本文提出在共炮检距剖面上进行二维弹性波记录的偏移方法。具体做法是，首先用弹性波方程将两分量的地震记录分解为Ｐ－Ｐ（或ＳＶ－ＳＶ）波和Ｐ－ＳＶ（或ＳＶ－Ｐ）波，然后用我们已导出的适合于处理共炮检距剖面的叠前偏移方法对它们进行处理。用模拟数据进行的试验说明方法正确，效果良好。  相似文献   

Evidence of chromosomal damage in common eiders (Somateria mollissima) from the Baltic Sea     

Matson CW  Franson JC  Hollmén T  Kilpi M  Hario M  Flint PL  Bickham JW 《Marine pollution bulletin》2004,49(11-12):1066-1071

Common eiders nesting in the Baltic Sea are exposed to generally high levels of contaminants including potentially genotoxic polycyclic aromatic hydrocarbons and organochlorines. Blood samples were collected from eiders at eight sites in the Baltic Sea and two sites in the Beaufort Sea. DNA content variation was estimated using the flow cytometric method, and subsequently utilized as a biomarker of genetic damage. We observed no significant differences in genetic damage among populations within either the Baltic or Beaufort Seas. However, eider populations from the Baltic Sea had significantly elevated estimates of genetic damage compared to populations from the Beaufort Sea.  相似文献   

Molecular cloning and mRNA expression of cytochrome P4501A1 and 1A2 in the liver of common minke whales (Balaenoptera acutorostrata)     

Satoko Niimi  Michio X. Watanabe  Eun-Young Kim  Hisato Iwata  Genta Yasunaga  Yoshihiro Fujise  Shinsuke Tanabe 《Marine pollution bulletin》2005,51(8-12):784

This study presents full-length cDNA sequences of CYP1A1 and 1A2, in common minke whale (Balaenoptera acutorostrata) from the North Pacific. Both CYP1A1 and CYP1A2 cDNAs had an open reading frame of 516 amino acid residues, and predicted molecular masses were 58.3 kDa and 58.1 kDa, respectively. The deduced full-length amino acid sequence of CYP1A1 revealed higher identities with those of sheep (86%) and pig (87%), and that of CYP1A2 was most closely related to human (82%) and monkey CYP1A2 (82%) among species from which CYP1A2 has been isolated so far. Differences in certain conserved and functional amino acid residues of CYP1A1 and 1A2 between common minke whale and other mammalian species indicate the possibility of their specific metabolic function. Concentrations of organochlorine compounds (OCs) including PCBs and DDTs analyzed in common minke whale liver showed no significant correlation with hepatic mRNA expression levels of CYP1A1 and CYP1A2, indicating no induction of these enzymes by such OCs.  相似文献   

中国大陆科学钻探100-2000m超高压变质岩普通Pb同位素地球化学初步研究——俯冲陆壳内脱耦的证据   总被引：1，自引：0，他引：1  

董锋李曙光  李秋立刘贻灿 陈福坤 《岩石学报》2006,22(7):1791-1798

本文首次对中国大陆科学钻探工程100～2000m榴辉岩中的绿辉石和片麻岩中的长石进行了普通Pb同位素研究。钻孔上部岩段（0-800m）样品放射成因Pb同位素含量比较高且变化幅度较小（^206Pb／^204Pb=16．81-17．29，^207Pb／^204Pb=15．37-15．45，^208Pb／^204Pb=37．20-37．66），说明其原岩的U／Pb和Th／Pb或者初始Pb同位素比值比较高。钻孔中部岩段（800-1600m）发育有韧性剪切带，相应的Pb同位素比值开始降低且有起伏（^206Pb／^204Pb=16．26-16．72，^207Pb／^204Pb=15．27～15．38，^208Pb／^204Pb=36．73～37．40）、钻孔下部岩段（800～1600m）样品放射成因Ph同位素含量低但变化幅度较小（^206Pb／^204Pb=16.05～16．46，^207Pb／^204Pb：15．22～15.29，^208Pb／^204Pb=36．68～37．48），说明其原岩的U／Pb和Th／Pb或者初始Ph同位素比值比较低。实测数据同模拟数据对比表明，钻孔上、下两岩段样品普通Ph同位素不同一方面是由于继承了原岩初始Pb同位素不同，另一方面是由它们的μ值不同所致。结合氧同位素研究，本文认为800m以上和1600m以下分属上地壳和中地壳．前者是从俯冲大陆岩石圈解耦的上地壳，在大陆岩石圈俯冲过程中，上部岩片同俯冲的大陆岩石圈发生挤离，率先沿着这一个挤离面逆冲折返至浅部。下岩片是随后从俯冲岩石圈解耦的中地壳岩片，并折返至浅部下伏在先期折返的上部岩片之下。考虑到中部岩段（800～1600m）既发育有韧性剪切带又具有异常低^18O峰值（Xiao et al．，2006），挤离面是在地壳内部晚元古代的断裂带及冷水活动通道基础上发育起来的。  相似文献   

Evaluation of two direct immunoassays for rapid detection of petroleum products on marine birds     

Fritcher DL  Mazet JA  Ziccardi MH  Gardner IA 《Marine pollution bulletin》2002,44(5):388-395

To reduce rehabilitation time and improve survival, a practical, objective test is needed to determine if marine birds are contaminated with oil before they enter captivity. The RaPID Assays and EnviroGard immunoassays for detection of polycyclic aromatic hydrocarbons (PAHs) in soil were evaluated for their ability to detect petroleum on feather samples from 30 intermediate fuel oil contaminated and 30 uncontaminated common murres (Uria aalge). Sensitivity, specificity, positive and negative predictive value, precision, and time required to run each assay were determined. The RaPID Assays was 96.7% sensitive and specific, while the EnviroGard assay was 93.3% sensitive, and 90.0% specific. Sensitivity decreased at higher dilutions for both assays. Intra-replicate variation was less than 20%. Our evaluation showed that these immunoassays are rapid and cost-effective methods for detecting oil-contamination on the plumage of seabirds, with the EnviroGard assay being more practical in most oil spill response situations due to ease of use and rapidity of results.  相似文献   

数字图书馆的公共性反思     

黄磊  陈慧 《国土资源信息化》2008,(4):3-5,14

本文在把握数字图书馆的基本特征前提下,运用公共性理论分析研究数字图书馆的公共性,通过分析数字图书馆的公共性特征及影响因素,发现许多非技术性的因素影响数字图书馆的进程,甚至违背了数字图书馆的服务宗旨,理想与现实之间存在着很大差距。  相似文献   

Existence of Microsatellites in Expressed Sequence Tags of Common Carp （Cyprinus carpio L. ） Available in GenBank dbEST Database     

HU Jingjie WANG Xiaolong HU Xiaoli BAO Zhenmin 《中国海洋大学学报(英文版)》2006,5(1):12-20

Common carp expressed sequence tags （ESTs） were analyzed for the existence of microsatellites, or simple sequence repeats （SSRs）. In the NCBI dbEST database, a total of 10612 sequences were registered before December 31, 2004. A complete search of 2-6 nucleotide microsatellites resulted in the identification of 513 SSR-containing ESTs, accounting for 4.8% of the total. Cluster analysis indicated that 73 sequences of SSR-containing ESTs fell into 27 groups and the remaining 440 ESTs were independent. A total of 467 unique SSR-containing ESTs were identified. These EST-SSRs contained a vari- ety of simple sequence types, and di- and tri-nucleotide repeats were the most abundant, accounting for 42.1% and 27.9% of the whole, respectively. Of the dinucleotide repeats, CA/TG was the most abundant, followed by GA/TC. BLASTx search showed that 38.1% of the SSR loci could be associated with genes or proteins of known or unknown function. BLASTx searches of SSR-containing ESTs also showed high frequencies （98/179） of hits on zebrafish sequences.  相似文献   

Application of the Common Offset Seismic Reflection Method to Urban Active Fault Surveys     

Liu Baojin 《中国地震研究》2007,21(2):196-203

The method and principle of common offset seismic surveys as well as the field data gathering and processing technique were introduced briefly. Through two urban active fault survey examples in Fuzhou and Shenyang, the efficiency and limitation of using the common offset seismic reflection technique to carry out urban active fault surveys were probed. The results show that this technique has the properties of high resolving power, better reconstruction of subsurface structures, and real-time analyzing and interpretation of investigation results on site. This method can be used to quickly locate objects under investigation accurately in the areas with thinner Quaternary overburdens and strong bedrock interface fluctuations.  相似文献