谁第一个发现DNA双螺旋结构     

田地 《发现．图形科普》2002,(3):62-65

  相似文献   

地质体中主要生物分子的研究方法及应用   总被引：4，自引：0，他引：4  

杨洪  程安进 《地质论评》1998,44(1):44-51

现代分子生物学,生物化学及有机地球化学等领域的发展,为古生物研究者在发子水平上探索地质生物世界提供了新的概念和技术手段,本文着重讨论了可用于分子古生物学研究的生物分子,例如,维管植物的木质素等稳定生物聚合物,古生物细胞内的类脂化合物,碳水化合物,蛋白质及氨基酸和含遗传信息的核酸,分子古生物资料可用于探讨化石埋藏学,古生物分子系统学,生物演化模式与机制,分子演化速率,古生态环境再造等问题,本文较详细  相似文献   

当今恐龙研究三大热点之我见     

钱迈平  厉建华 《江苏地质》1998,22(2):73-81,T001

根据近年来对辽宁义县晚侏罗－早白垩世恐龙和鸟类化石及河南西峡晚白垩世恐龙蛋化石的初步研究就目前世界恐龙研究前沿领域涉及的三大热点问题一提取ＤＮＡ,温血还是冷血及是龙还是鸟的争议情况研究现状提出一些粗浅的看法。  相似文献   

冲绳日本绒螯蟹线粒体DNA 12S rRNA序列的研究   总被引：2，自引：0，他引：2  

张秀梅  高天翔  王溪宏 《广东海洋大学学报》2000,20(2):11-14

采集日本冲绳源河川和边野古川的日本绒螯蟹样品 ,参考果蝇与蚤状氵蚤线粒体 DNA12 Sr RNA基因片段序列进行了其相同片段的引物设计、PCR扩增及序列测定。结果表明冲绳两河川 4只日本绒螯蟹的碱基序列完全相同 ,为 4 58bp,其 A、T、G、C含量分别为 16 0 bp(34.94 % )、 179bp(39.0 8% )、4 9bp(10 .70 % )、70 bp(15.2 8% ) ,同果蝇与蚤状氵蚤相同基因片段的序列含量相似。  相似文献   

Preliminary Study on Applicability of Microsatellite DNA Primers from Parasite Protozoa Trypanosoma cruzi in Free-living Protozoa   总被引：1，自引：0，他引：1  

ZHANGWenjing YUYuhe SHENYunfen MIAOWei FENGWeisong 《中国海洋大学学报(英文版)》2004,3(1):80-84

1　Introduction　Generallyknownasacodominantgeneticmarker ,microsatellitehasbeenwidelyusedinstudiesonpopu lationgenetics,high resolutiongenotyping ,genemap ping ,evolution ,linkageanalysis ,conservationbiology ,behaviouralecology ,relationsbetweenparasite…  相似文献   

海南近海野生鞍带石斑鱼群体遗传多样性的RAPD分析     

符书源  尹绍武  陈国华  乔淑军  许丽丽 《海洋通报》2008,27(3):25-31

用RAPD技术分析海南近海野生鞍带石斑鱼群体的遗传多样性,从48个随机引物中筛选出20个引物对鞍带石斑鱼的DNA进行扩增,结果共检测出131个位点,其多态位点比例(P)为48.09%;利用RAPDdistance1.04软件获得海南野生鞍带石斑鱼群体21个个体间的遗传距离矩阵,依此计算出个体间平均遗传距离(D)为0.2650,个体间平均遗传相似系数(S)为0.7350;利用POPGENE1.3软件计算出Shannon遗传多样性指数(H0)为0.2266;与其他种类的石斑鱼群体的RAPD分析结果比较可知,海南近海野生鞍带石斑鱼群体仍保持着较丰富的遗传多样性.因此在海南发展鞍带石斑鱼的养殖业,可以选择海南近海的野生鞍带石斑鱼作为亲本进行人工繁殖,但应采取一些有效的保护措施,以避免遗传多样性的丧失.  相似文献   

自杀者的童年阴影     

《海洋世界》2008,(6):7-7

来自加拿大麦吉尔大学的一个研究小组发现，那些以自杀来结束生命的人的大脑与正常人的大脑有很大不同。即使“自杀者”大脑和“非自杀者”大脑在基因序列上差别不大，它们在“表观遗传标记”方面却存在明显不同。表观遗传学研究的是基因功能的改变，而这种改变并不涉及DNA序列的变化。遗传自父母的DNA，其序列在人的一生中不会改变，身体各部位的DNA序列相同。  相似文献   

Characterization of marine microplankton communities of Qingdao coastal areas using randomly amplified polymorphic DNA（RAPD）   总被引：1，自引：1，他引：0  

GAO Shan  HU Xiaozhong  CHEN Zigui  XU Henglong  YI Zhenzhen  LIN Xiaofeng  LIJiqiu 《海洋学报(英文版)》2009,28(1)

Microplankton communities of three coastal sites of Qingdao, Shandong Province, China were investigated using RAPD (random amplified polymorphic DNA) molecular markers and morphological observations. Eight RAPD-primers were selected to amplify the DNA polymorphy. The genetic distances inferred from the pairwise similari-ties were calculated for the phylogenetic tree construction. Meantime, the traditional microscopic determination, a way of visualizing the species composition, was performed to detect the major taxa of microplanktons from all samples. Results showed that: (1) the band sharing index values were in the range of 0. 504 2-0. 763 2 among samples from the same sampling site at different time scales, while 0.406 5-0.685 7 among the samples from different stations at the same time scales, indicating that spatial variations of microplankton communities were more pronounced than temporal ones; (2) samples from the same station basically clustered together, cor-responding to the geographic distribution of the sampling sites; (3) diversity derived from genetic and morpho-logical data did not correspond with each other well.  相似文献   

Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency     

Aibin Zhan  Zhenmin Bao  Xiaoli Hu  Wei Lu  Jingjie Hu 《中国海洋大学学报(英文版)》2009,8(2):161-165

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.  相似文献   

Species delimitation in the green algal genus Codium (Bryopsidales) from Korea using DNA barcoding     

LEE Hyung Woo  KIM Myung Sook 《海洋学报(英文版)》2015,34(4):114-124

Codium, one of the largest marine green algal genera, is difficult to delimit species boundary accurately based on morphological identification only. DNA barcoding is a powerful tool for discriminating species of seaweeds. The plastid elongation factor TU(tuf A) is considered as maker to perform DNA barcoding of green algal species than rbc L gene due to universality and rapid evolution rate. We conducted DNA barcoding application to Codium specimens from the Jeju Island, Korea to overcome the limit of morphological identification and to confirm the species diversity. As a result of applying tuf A marker, we newly generated fifty-five tuf A barcodes to resolve eight species. Tuf A marker exhibited 6.1%–21.8% interspecific divergences, wider than the gap of rbc L exon 1,3.5%–11.5%. Molecular analysis of rbc L exon 1 sequences of Codium revealed eight distinct species like tuf A analysis separated in five phylogenetic groups. DNA barcoding of the genus Codium using tuf A marker is more helpful to overcome the limit of morphological identification, and this is more potential to reveal cryptic species and to resolve the relationships among subspecies than rbc L analysis alone. The complement of tuf A barcoding and rbc L analyses including morphology for the genus Codium in the northwestern Pacific will give much more reliable achievement for discovering species diversity and resolving the phylogenetic relationships.  相似文献