海产鱼类中异尖线虫酶消化检测技术的研究与应用     

许旭  黄维义  隋建新  林洪  鲜均章  励建荣  曹立民 《中国海洋大学学报(自然科学版)》2010,40(3)

以3种海鱼(牙鲆、狭鳕、鲱鱼)为样本,针对海产品中主要的致病性寄生虫-异尖线虫,开展了酶消化检测技术的研究.根据鱼肉消化前后干物质的质量变化设计了胃蛋白酶消化效率的计算方法,并按照鱼肉:消化液＝1:10 (g/mL)的反应比例,分别确定了酶水解鱼肉的最佳条件为:初始pH值为1.1,温度为37 ℃,酶活力为8 U/mL左右;消化后所得的虫体采用多重PCR方法替代传统的形态学观察进行种属鉴定,从而初步建立了基于酶水解-多重PCR的异尖线虫的酶消化检测体系.实际样本检测表明,该技术可以较为准确、快速地用于海产鱼类中简单异尖线虫(Anisakis simplex)和伪地新线虫(Pseudoterranova decipiens)的确证性检测.  相似文献   

两种体色三疣梭子蟹亲缘关系的PCR-SSCP分析     

迟大利  高焕  沈颂东  阎斌伦 《海洋通报(英文版)》2010,12(1):79-88

三疣梭子蟹作为重要的海洋经济动物，其常见体色为茶绿色。近年来在我国东部沿海海域海捕三疣梭子蟹中开始出现体色为紫色的一类梭子蟹，除体色不同外，二者表型特征并无显著差异。为确定两种体色三疣梭子蟹之间的关系，本文对两种体色三疣梭子蟹不同个体的线粒体COl和16srRNA基因进行了比较分析以判定体色的差异是否由于种属的差异引起的。通过对以上两个基因部分序列的SSCP和相关序列测定分析，发现在紫色和茶绿色群体中这两个线粒体保守序列基因的SSCP电泳条带图谱相似，序列分析表明COl和16srRNA基因在两种体色三疣梭子蟹群体中的核苷酸序列相似度分别为99．81％和99．91％。以上结果表明，紫色三疣梭子蟹并未发生亚种的分化，即紫色和茶绿色群体属于同一个种。此外，紫色梭子蟹群体的线粒体DNA序列在进化关系上较茶绿色群体更为保守。  相似文献   

The control of maturity and kerogen type on quantitative analytical pyrolysis data     

Kjell ygard  Steve Larter  Joe Senftle 《Organic Geochemistry》1988,13(4-6)

Quantitative pyrolysis-gas chromatography has been performed on 96 kerogen samples isolated from 17 wells on the Norwegian Continental shelf. Petrographic and bulk geochemical measurements were also performed on the samples, and a combined data set of 117 variables for each sample was analysed using principal components analysis (PCA). This approach provides an objective and reproducible means of kerogen characterisation, which can be easily automated. In addition to objective kerogen characterisation and facile visualisation of facies and maturity related chemical trends, the method has the potential to allow objective prediction of key geochemical parameters such as maturity level from pyrogram data.  相似文献   

利用原生岩浆定量反演原岩微量元素丰度的方法   总被引：1，自引：0，他引：1  

杨晓松 《岩石矿物学杂志》1994,13(1):33-41

利用同源不同程度平衡部分熔融作用形成的两种原生岩浆岩的微量元素浓度，提出了一种定量反演原岩微量元素丰度的方法。反演前不需要做任何人为假设，为定量研究源区的微量元素地球化学提供了一个有力的手段。对汉诺坝新生代橄榄拉斑玄武岩和碱性玄武岩的成因及地幔源区特征作了讨论。反演结果显示本区地幔相对球粒陨石有过明显的ＲＥＥ富集过程，并且不同的ＲＥＥ富集程度存在显著的差异。  相似文献   

Advanced use into rainfall prediction of three-dimensionally scanning radar   总被引：1，自引：0，他引：1  

E. Nakakita  S. Ikebuchi  M. Shiiba  T. Takasao 《Stochastic Environmental Research and Risk Assessment (SERRA)》1990,4(2):135-150

A computational method for the determination of rainfall distribution for applications in short term rainfall prediction is presented here. The method is strongly influenced by the experience gained from the observation and analysis of data gathered on a heavy rainfall event in 1986 that occurred during the Baiu Season in Japan. The method is based on the concept that rainfall occurs as an interaction between an instability field, appropriately modeled, and a field of water vapor under the influence of topography. The results from this computational method showed good agreement with the temporal variation in the rainband that moved across the observation field in 1986. Towards determination of the parameters in the computational model, another method for the determination of the rainfield is also developed. This second method determines the rainfall distribution from estimation of the conversion rate of water vapor to liquid water through use of data from a three dimensional scanning radar. The results are consistent with those obtained from the first method.  相似文献   

Applying　artificial　neural　networks　to　achieve　quantitative　division　of　potential　focal　regions     

姜效典  张维岗 《地震学报(英文版)》1994,7(4):645-652

（姜效典，张维岗）Ａｐｐｌｙｉｎｇａｒｔｉｆｉｃｉａｌｎｅｕｒａｌｎｅｔｗｏｒｋｓｔｏａｃｈｉｅｖｅｑｕａｎｔｉｔａｔｉｖｅｄｉｖｉｓｉｏｎｏｆｐｏｔｅｎｔｉａｌｆｏｃａｌｒｅｇｉｏｎｓ￥Ｘｉａｏ－ＤｉａｎＪＩＡＮＧａｎｄＷｅｉ－ＧａｎｇＺＨＡＮＧ（...  相似文献   

扫描电镜的定量体视测量法     

冯有纲  汪大成 《矿物学报》1985,(3)

本文提出了一种新的体视学原理及其在扫描电子显微镜中的应用。借助于它的实验技术,可从图象中获得大量信息,而且能测定图片上任何物体的真实尺寸与几何图形,这种方法具有如下的新功能:1.可测定小晶面夹角;2.可测定图片上任意两点之间的真实距离;3.可测定曲线的长度和曲面的面积。文中例举了该法应用于地学的三例.  相似文献   

Quantitative chemical characterization of vitrinite concentrates using pyrolysis-gas chromatography. Rank variation of pyrolysis products     

J.T. Senftle  S.R. Larter  B.W. Bromley  J.H. Brown 《Organic Geochemistry》1986,9(6)

A series of hand-picked vitrinite samples from the Lower Kittanning Seam, Pennsylvania have been examined using quantitative pyrolysis-gas chromatography. These vitrinites ranged in rank from 0.59 to 1.71% reflectance, a rank range from high volatile C bituminous to low volatile bituminous. High molecular weight pyrolysis products included alkyl aromatic and phenolic compounds. Attempts have been made to correlate the pyrolysis product composition to rank parameters including vitrinite reflectance, volatile matter yield, carbon content, atomic H/C ratio and Rock-Eval determined T_max. Total yield of phenols was found to be strongly and inversely rank related. A clear relationship between C₈ alkyl-benzene yield and rank was not found for the sample set.  相似文献   

地下动态导体充电法高精度定量解释   总被引：2，自引：0，他引：2  

何裕盛 《物探与化探》2001,25(3):215-222

提供了动态导体充电法的高精度定量解释的原理和具体方法。  相似文献   

川藏公路然乌-鲁朗段地质灾害影响因素定量分析     

丁继新  尚彦军  杨志法  尹俊涛 《水文地质工程地质》2005,32(4):1-5

川藏公路然乌—鲁朗段工程地质条件极其复杂,导致大型地质灾害频繁发生。为了定量地分析各种影响因素对大型地质灾害的贡献,本文根据信息熵的理论和方法,提出了“地质灾害熵”的概念。通过详细分析川藏公路然乌—鲁朗段大型地质灾害资料,选择平均坡度、断层和节理、岩体结构、松散堆积物、水文地质条件、冰川作用和降雨等7个影响因素,分析它们对研究区某些大型地质灾害的影响程度,计算出了各影响因素的“地质灾害熵”。根据“地质灾害熵”计算得到了各影响因素的权重,在一定程度上解决了地质灾害影响因素的定量分析问题。  相似文献