Effects of dietary soybean stachyose and phytic acid on gene expressions of serine proteases in Japanese flounder (Paralichthys olivaceus)     

Haifeng Mi  Kangsen Mai  Wenbing Zhang  Chenglong Wu  Yinghua Cai 《中国海洋大学学报(英文版)》2011,10(3):234-240

Soybean stachyose (SBS) and phytic acid (PA) are anti-nutritional factors (ANF) which have deleterious effects on the growth and digestibility in fish. The present research studied the effects of dietary SBS and PA on the expression of three serine protease genes in the liver of Japanese flounder (Paralichthys olivaceus). These genes are trypsinogen 1 (poTRY), elastase 1 (poEL) and chymotrypsinogen 1 (poCTRY). Eight artificial diets with graded levels of supplemented ANFs were formulated to 4 levels of SBS ...  相似文献   

鸡EX-FABP基因遗传变异及与肌内脂肪含量的相关分析     

张丽  赵志远  杜炳旺  江新生  吴薇薇 《广东海洋大学学报》2011,31(3):75-79

利用PCR-SSCP结合DNA测序方法,对贵妃鸡、怀乡鸡及二者的杂交F1代共计360个个体EX-FABP基因遗传特征及品种差异进行研究,发现2773T>C,2867 C>T和2913 G>A三个位点在贵妃鸡中表现为单态,在怀乡鸡和杂交F1群体中表现为多态,这3个位点有望作为贵妃鸡、怀乡鸡乃致其他品种鸡种质资源鉴定的参考依据。对比分析3个群体胸肌肌内脂肪含量(IMF)表明,怀乡鸡和杂交鸡母鸡的IMF显著高于贵妃鸡;而3个群体公鸡间的IMF差异不显著;除贵妃鸡外,同一品种内公鸡的IMF均小于母鸡(P<0.05)。多态位点与鸡胸肌肌内脂肪含量的相关性分析结果表明,在2773位点,TC基因型个体的IMF值大于TT基因型;在2913位点,AA基因型个体的IMF值大于GG基因型,但没有达到显著水平(P>0.05)。  相似文献   

红笛鲷α-珠蛋白和β-珠蛋白基因的克隆及序列分析     

张新中  鲁义善  吴灶和  简纪常 《广东海洋大学学报》2011,31(3):1-6

应用已知的标签序列通过RACE-PCR和RT-PCR方法成功克隆红笛鲷(Lutjanus sanguineus)α-珠蛋白和β-珠蛋白基因的全长cDNA序列。α-珠蛋白和β-珠蛋白基因的cDNA全长分别为560和563 bp,开放阅读框分别为429和444 bp,各编码143和148个氨基酸,理论相对分子质量分别为15690和16400,理论等电点为7.79和6.61。氨基酸序列BLAST结果表明,红笛鲷α-珠蛋白基因和β-珠蛋白基因编码的氨基酸序列与其他鱼类的相似性分别在59%~79%和55%~80%之间。用邻接法(Neighbor-Joining)构建的α-珠蛋白基因和β-珠蛋白基因的系统发育树表明,红笛鲷与真鲷(Pagrus major)、金头鲷(Sparus aurata)和鰤(Seriola quinqueradiata)等亲缘关系较近。  相似文献   

三角鲤胚后发育研究     

马桂玉  李坚明  梁军能 《广东海洋大学学报》2011,(4)

三角鲤的胚后发育过程可分为早期仔鱼、晚期仔鱼和稚鱼3个阶段共11个时期。与其他鲤科鱼类比较,三角鲤胚后发育具卵黄囊吸收迅速、鳔迅速发育成形(巡游模式迅速建立)及鳞片的较早出现、长齐和完成胚后发育时间较短等特点;在水温为25.0±0.7℃的条件下,全长5.2±0.2 mm的初孵仔鱼经过24～26 d的发育,全长达20.6±0.3 mm即完成胚后发育;仔鱼、稚鱼的全长随日龄呈线性生长,相关方程式为L=5.8591+0.6254 A(r=0.999 2,P<0.01),每日增长约为0.63 mm。根据三角鲤胚后发育特点讨论了鱼苗生产应注意的问题。  相似文献   

Wood productivity of Prosopis flexuosa D.C. woodlands in the central Monte: Influence of population structure and tree-growth habit     

J.A. Alvarez  P.E. Villagra  R. Villalba  M.A. ConyM. Alberto 《Journal of Arid Environments》2011,75(1):7-13

A balance between forest production and protection is hard to achieve in arid zones due to their low potential for wood production. Prosopis flexuosa woodlands are the major woody formations in the Monte desert and are currently in a degraded state due to intense use. The main degradation factors in the study area are overgrazing and firewood extraction. We developed allometric models to estimate the aerial biomass of P. flexuosa, compared annual growth rates of one- and multi-stemmed individuals through dendrochronological methods, and estimated the productivity of four structurally different woodlands in the central Monte. Total dry weight was best estimated by power equations. Annual increments in basal area and dry weight were initially larger for multi- than one-stemmed individuals. However, whereas multi-stemmed individuals rapidly decreased their growth rates after 60 years of age, one-stemmed trees maintained steady growth rates during the first 100 years. Depending on woodland density and tree size, total woodland biomass varied between 4000 and 15 000 kg ha⁻¹. Wood productivity was similar in all four woodlands studied (121.6-173.7 kg ha⁻¹ year⁻¹). Our results reveal the importance of tree growth habit to productivity, and suggest that regulated extraction of firewood and poles from old multi-stemmed individuals could optimize wood productivity and contribute to the sustainable use and conservation of these woodlands.  相似文献   

秦皇岛市气温与近地面臭氧浓度的关系分析     

徐静 《气象与环境学报》2020,36(4):83-88

利用2013年1月至2014年12月和2017年1月至2019年6月秦皇岛市近地面臭氧（O₃）浓度数据和气象资料,采用广义相加模型（GAM）,运用回归分析方法和基于R语言的统计分析软件,控制气压、相对湿度、日照时数、总云量等要素的混杂效应及时间变化趋势,分析春季、夏季、秋季、冬季气温与O₃浓度的关系。结果表明：秦皇岛市O₃浓度夏季最高、春季次之,冬季最低,与气温变化趋势基本一致,呈现明显的季节变化。各季气温与O₃浓度呈非线性相关关系,拟合曲线存在拐点,拐点两侧相关效应存在明显差异,主要表现为春季日平均气温高于15.0℃时,气温每升高1℃,O₃浓度增加7.6 μg·m^-3,增长速率是气温低于15.0℃时的4.0倍;夏季日平均气温高于27.2℃时,气温每升高1℃,O₃浓度增加13.9 μg·m^-3,增长速率是气温低于27.2℃时的11.6倍;秋季日平均气温高于21.4℃时,气温每升高1℃,O₃浓度增加47.5 μg·m^-3,增长速率是气温低于21.4℃时的19.1倍;冬季O₃浓度偏低且变化较为平稳,气温对O₃浓度的变化影响不大。由于春夏两季O₃浓度基础值偏高,因此,夏季和春季气温偏高时O₃浓度快速增加现象应引起高度重视。  相似文献   

Spartina densiflora demonstrates high tolerance to phenanthrene in soil and reduces it concentration     

Redondo-Gómez S  Andrades-Moreno L  Parra R  Valera-Burgos J  Real M  Mateos-Naranjo E  Cox L  Cornejo J 《Marine pollution bulletin》2011,62(8):1800-1808

The present study was conducted to investigate the tolerance of Spartina densiflora to phenanthrene, and to test its ability in phenanthrene dissipation. A glasshouse experiment was designed to investigate the effect of phenanthrene from 0 to 1000 mg kg⁻¹ on growth and photosynthetic apparatus of S. densiflora by measuring chlorophyll fluorescence parameters, gas exchange and photosynthetic pigments. We also performed chemical analysis of plant samples, and determined the concentration of phenanthrene remaining in soil. S. densiflora survived to concentrations as high as 1000 mg kg⁻¹ phenanthrene in soil; in fact, there was no significant difference in RGR among the treatments after 30 days. Otherwise, phenanthrene affected photosynthetic apparatus at 100 and 1000 mg kg⁻¹; thus, the lower Φ_PSII could be explained by the declined photosynthetic pigment concentrations. Soil extraction indicated a more marked rate of phenanthrene disappearance in the soil in the presence of S. densiflora.  相似文献   

Timing of basin inversion on the Isle of Wight: New evidence from geophysical log correlation, seismic sections and lateral facies change in the Palaeogene Headon Hill Formation     

Andrew J. Newell  David J. Evans 《Proceedings of the Geologists' Association. Geologists' Association》2011,122(5):868-882

Paleogene thickness patterns across the Bouldnor Syncline and Porchfield Anticline in the northwestern Isle of Wight have been deduced using outcrop information, borehole correlation, gamma-ray logs and seismic reflection data. The thickness patterns provide evidence for an early phase of basin inversion at around the Bartonian-Priabonian boundary (Late Eocene) in the Isle of Wight. Paleogene strata older than the Becton Sand Formation show little evidence for significant lateral changes in thickness, even though the boreholes are located at various structural positions around the Bouldnor Syncline and Porchfield Anticline. In contrast, both seismic reflection and borehole data provide evidence for marked thinning of Paleogene strata onto the Porchfield Anticline at around the level of the Becton Sand Formation and basal Headon Hill Formation (Totland Bay Member) which probably results from an episode of basin inversion and growth folding. The inversion event was relatively minor and short-lived and continues to point toward the main phase of the basin inversion being late Oligocene or younger. However, it still has important implications for understanding structural control on sedimentation patterns in the Headon Hill Formation, with the migration of sandy channelised depositional systems into the axis of the Bouldnor Syncline, and the sequence stratigraphic significance of the important Bartonian-Priabonian regression event, which may related to tectonics rather than global sea-level change.  相似文献   

周期性饥饿再投喂对建鲤（Cyprinus carpio var．Jian）生长、体组成、消化酶的影响          下载免费PDF全文

乔秋实  蒋广震  刘文斌  夏薇  刘兆普 《海洋与湖沼》2011,42(3):367-373

采用周期性饥饿再投喂的方法,研究周期性饥饿再投喂对建鲤（1．5±0．2g）的生长、体组成、消化酶的影响。试验分为10个组,共进行60d。结果表明,在相同的饥饿天数下,延长再投喂天数可以使建鲤的增重率逐渐增加,而且S1F4、S2F8、S4F16组的增重率与对照组无显著性差异（P〉0．05）,9个周期性饥饿再投喂组在摄食期...  相似文献   

长心卡帕藻（Kappaphycus alvarezii）表达序列标记分析     

刘晨临  黄晓航  刘建国 《海洋与湖沼》2011,42(6):804-810

分别采用BlastX和Blast2go软件对453条非冗余的长心卡帕藻EST序列进行匹配分析和GO注释。结果表明,有281条序列与蛋白数据库中序列匹配,有132条序列被归属为3个子本体：分子功能、生物学过程和细胞组成。同时筛选到24个与逆境适应相关的基因,包括应对胁迫反应和抗氧化反应的基因。采用GCUA软件,进行长心卡...  相似文献