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In this study,we evaluated the anti-proliferative activity of phlorotannins derived from brown algae Laminaria japonica Aresch extracts on the human hepatocellular carcinoma cell (BEL-7402) and on murine leukemic cells (P388) by MTT assay.Cells were incubated with 100 μg/mL of the phlorotannin extract (PE) for 48 h.The inhibitory rate of PE on BEL-7402 and P388 cells was 30.20±1.16% and 43.44±1.86%,respectively,and the half-inhibitory concentration of PE (IC50) on P388 and BEL-7402 cells was 120 μg/mL and >... 相似文献
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为进一步研究海带自然病烂过程中生理生化的变化,引入了高等植物抗病研究中常用的3个指标:苯丙氨酸转氨酶(phenylalanina ammonia—lyse,PAL)、多酚氧化酶(polyphenol oxidase,PPO)和多酚。在实验生态条件下,对三者在海带病烂过程中的变化进行了研究,结果表明,在海带幼苗由健康到病烂的过程中,海带PAL活性总体表现为下降趋势;PPO活性先升高后降低;多酚含量为下降趋势。经相关性分析,PAL与多酚相关性不显著,而PPO与多酚显著负相关(r=-0.619,p=0.014)。结合前期工作认为,SOD和PPO的联合表现可作为指示海带病烂发生的生物学指标。 相似文献
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我国引种海带(Laminaria japonica)和长海带(L.longissima)配子体克隆的随机扩增多态性DNA分析 总被引:1,自引:0,他引:1
运用RAPD技术,对引种我国的海带(Laminaria japonica)和长海带(L.longissima)的22个配子体克隆进行分析。筛选的19条随机引物共获得252个位点.扩增片段大小在200~2000bp之间,多态性位点228个,占90.5%。配子体克隆遗传变异分析表明,长海带和海带的遗传多样性都比较高,两者之间的遗传分化较为明显,且种间变异大于种内变异。长海带和海带雄配子体克隆的遗传变异程度均低于雌配子体克隆。另外,两个种的雌、雄配子体之间均存在一定程度的分化,且长海带雌、雄配子体之间的遗传分化大于海带配子体。 相似文献
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Genetical study on the parthenogenesis in Laminaria japonica 总被引:3,自引:0,他引:3
The parthenogenesis and natural doubling of chromosomes in wild type female gametophytes of Laminaria japonica were studied. The results indicate that not all the female gametophytes from the wild type hybrid parent can propagate through parthenogenesis. Most parthenosporophytes can mature, their spores germinate into gametophytes, the latter then developed into female sporophytes. To form these parthenosporophytes the natural doubling of chromosomes occurred mainly at the first and second cell divisions of the spores. It is thus considered that the parthenogenesis of L. japonica is inheritable and the relative genes link closely with the genes controlling the natural doubling of chromosomes and the female determination. 相似文献
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Abstract. The possible effects of fine-grained deposits on the photosynthesis, growth, and nutrient uptake of Laminaria have been studied in field and laboratory experiments. Some of the plants were treated with fine-grained material for two hours and then transplanted in rows together with untreated plants. At two-week intervals the treatment was repeated and samples collected. The macroalgae were analyzed for growth as well as chlorophyll a , nitrogen, and phosphorus content. In the laboratory experiments, plants of a uniform size were incubated in Plexiglas containers after leaf-marking and treatment in a sediment suspension. At weekly intervals, photosynthesis, growth, chlorophyll a , and nutrients were analyzed.
The field study showed that Laminaria growth was significantly affected by the fine-grained material. The growth in the control experiment was 20% higher than in the treated plants. The chlorophyll a concentrations in the treated plants were significantly lower than in the control experiment, and the results indicated a reduced nutrient uptake in the treated plants. In the laboratory experiments the growth of the treated plants was only 30% of the controls, but no differences in photosynthetic capacities were detected. Nitrogen uptake was reduced in the treated plants, but no effects were observed on phosphorus uptake. Thick layers of fine-grained material caused direct harmful effects and rotting.
The results are discussed in relation to dredging activities; it is concluded that in heavy sedimentation areas, Laminaria growth may be significantly reduced beyond the indirect effect of shading from suspended material in the water. 相似文献
The field study showed that Laminaria growth was significantly affected by the fine-grained material. The growth in the control experiment was 20% higher than in the treated plants. The chlorophyll a concentrations in the treated plants were significantly lower than in the control experiment, and the results indicated a reduced nutrient uptake in the treated plants. In the laboratory experiments the growth of the treated plants was only 30% of the controls, but no differences in photosynthetic capacities were detected. Nitrogen uptake was reduced in the treated plants, but no effects were observed on phosphorus uptake. Thick layers of fine-grained material caused direct harmful effects and rotting.
The results are discussed in relation to dredging activities; it is concluded that in heavy sedimentation areas, Laminaria growth may be significantly reduced beyond the indirect effect of shading from suspended material in the water. 相似文献
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为更好地了解海带病烂的发生机制,针对褐藻酸降解菌埃氏交替单胞菌(Altermonas espejiana)菌株A1的生长及产酶条件进行了初步研究,并用不同浓度的菌液对健康海带进行了感染试验,测定了其体内可溶性蛋白质和可溶性糖含量的变化。结果表明,该菌株生长和产酶的最适条件为:20℃,0.5%~0.6%褐藻酸钠,pH=7,5,氮源为(NH4)2S04,培养时间72h。此外,感染菌株A1后,海带体内可溶性蛋白质和可溶性糖的含量均增加,且随着感染菌液浓度的升高,海带体内可溶性蛋白质含量升高,而可溶性糖含量先升高后降低。 相似文献
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海带是我国海水养殖主要品种之一,其综合应用价值较高[1].20世纪90年代末期,海带配子体克隆在海带苗种繁育中的应用,为海带育苗生产开辟了新途径[2].主要运用生长发育调控技术实现海带配子体克隆细胞大量扩增和同步成熟来繁育海带优良苗种,这样可大幅度降低育苗成本,缩短育苗周期,该项技术具有良好的应用前景.目前海带配子体克隆育苗技术尚未在海带育苗生产中得到规模化应用,其作为一项高新技术,部分关键技术仍有待进一步解决.在与有关生产单位进行海带配子体克隆育苗实验的工作中,笔者认为克隆细胞的附着是影响海带商品苗质量(出苗密度及出苗均匀程度)的关键问题之一. 相似文献