Effects of Pseudoalteromonas sp. BC228 on digestive enzyme activity and immune response of juvenile sea cucumber (Apostichopus japonicus)     

Yuexin Ma  Feixue Sun  Congyao Zhang  Pengyun Bao  Shuqing Cao  Meiyan Zhang 《中国海洋大学学报(英文版)》2014,13(6):1061-1066

A marine bacterium, Pseudoalteromonas sp. BC228 was supplemented to feed in a feeding experiment aiming to determine its ability of enhancing the digestive enzyme activity and immune response of juvenile Apostichopus japonicus. Sea cucumber individuals were fed with the diets containing 0(control), 105, 107 and 109 CFU g-1 diet of BC228 for 45 days. Results showed that intestinal trypsin and lipase activities were significantly enhanced by 107 and 109 CFU g-1 diet of BC228 in comparison with control(P 0.01). The phagocytic activity in the coelomocytes of sea cucumber fed the diet supplemented with 107 CFU g-1 diet of BC228 was significantly higher than that of those fed control diet(P 0.05). In addition, 105 and 107 CFU g-1 diet of BC228 significantly enhanced lysozyme and phenoloxidase activities in the coelomic fluid of sea cucumber, respectively, in comparison with other diets(P 0.01). Sea cucumbers, 10 each diet, were challenged with Vibrio splendidus NB13 after 45 days of feeding. It was found that the cumulative incidence and mortality of sea cucumber fed with BC228 containing diets were lower than those of animals fed control diet. Our findings evidenced that BC228 supplemented in diets improved the digestive enzyme activity of juvenile sea cucumber, stimulated its immune response and enhanced its resistance to the infection of V. splendidus.  相似文献   

Marine bacterium strain screening and pyrethroid insecticide-degrading efficiency analysis     

孙爱丽  刘菁华  史西志  李德祥  陈炯  唐道军 《中国海洋湖沼学报》2014,32(5):1029-1035

A pyrethroid insecticide-degrading bacterium, strain HS-24, was isolated from an offshore seawater environment. The strain, which can degrade cypermethrin(CYP) and deltamethrin(DEL), was identified as Methylophaga sp. The optimal culture and degradation conditions for CYP and DEL by strain HS-24 is pH 7 at 28°C. Under optimum culture conditions, strain HS-24 exhibited a broad degradation concentration range of 100, 200, 400, 600, and 800 mg/L for CYP and DEL. The metabolic intermediates were analyzed by NMR, which provided strong evidence that CYP and DEL removal occurred mainly because of a biological process. The toxicity of the degradation products of strain HS-24 was studied simultaneously by measuring the light output of the luminescence bacterium. This demonstrated that the biodegradation ability of strain HS-24 significantly decreased the toxicity of CYP- and DEL-contaminated aquaculture seawater. Finally, the findings of this paper indicate that strain HS-24 is thus revealed as a biological agent for the remediation of marine aquatic environments.  相似文献   

温度和光径对微绿球藻生长及营养成分含量的影响     

杜晓凤  邹宁  孙东红  常林  赵萍 《海洋科学》2014,38(4):50-54

研究了温度和光生物反应器的光径对微绿球藻(Nannochloropsis oculata Hibberd)生长及细胞内几种营养成分含量的影响。结果表明,温度对微绿球藻的生长有显著影响(P0.05),对几种营养成分的含量有极显著影响(P0.01),在28℃的培养条件下,细胞生长速度最快,此时蛋白质含量最高,为5.66%,多糖含量最低,仅为1.47%,但最大生物产量却是在30℃的条件下获得,总脂与叶绿素a的含量随温度的升高而降低,类胡萝卜素含量随温度升高而增大。光生物反应器的光径对微绿球藻的生长和总脂、可溶性蛋白、色素的含量有极显著影响(P0.01),对多糖含量有显著影响(P0.05),比生长速率、最高细胞密度和单位体积产量均随光径的增大而减小,单位面积产量随光径的增大而增大;总脂、可溶性蛋白和多糖的含量随光径的增大而降低,叶绿素和类胡萝卜素含量随光径增大而增大,类胡萝卜素含量的增大趋势比叶绿素更明显。  相似文献   

A new genus of Sycoracinae (Diptera: Psychodidae) from Upper Cretaceous amber of New Jersey     

《Cretaceous Research》2015

We describe herein a new genus and species of Sycoracinae (Xenosycorax engeli). This new taxon is characterized, described, illustrated and its taxonomic position is discussed. This discovery is very interesting for the understanding of the evolution of this group.  相似文献   

A new Osmylidae (Insecta,Neuroptera) from the Lower Cretaceous Crato Formation in Brazil     

《Cretaceous Research》2015

Cratosmylus magnificus gen. et sp. nov., type genus and species of the new osmylid subfamily Cratosmylinae, is described from the Lower Cretaceous of the Crato Formation, Brazil. The relationships of this taxon remain uncertain within the Osmylidae, mainly due to the lack of phylogeny of the whole family. Nevertheless, it shares with the Mesozoic subfamily Saucrosmylinae the Rs curved and bent anteriorly distally, even in a more pronounced state, i.e. Rs touching R1 in its distal part.  相似文献   

Rock Stress,Rock Stress Measurements,and the Integrated Stress Determination Method (ISDM)     

D. Ask  O. Stephansson  F. H. Cornet  M. V. S. Ask 《Rock Mechanics and Rock Engineering》2009,42(4):559-584

The primary objectives of this work are to (1) improve the understanding of the prevailing stress distribution at the Äspö Hard Rock Laboratory (HRL) in SE Sweden by employing an integrated stress determination approach, and in order to accomplish this (2) extend the existing stress integration methodology denominated integrated stress determination method (ISDM; Cornet in Comprehensive Rock Engineering vol 3, Pergamon Press, Oxford, pp 413–432, 1993a). The new developments of the ISDM involve a 12-parameter representation of the regional stress field in the rock mass (i.e., the full stress tensor and its variation with depth) that is applicable to hydraulic stress data (sleeve fracturing, hydraulic fracturing, and hydraulic tests on pre-existing fractures), overcoring data (CSIR- and CSIRO-type of devices), and to combinations of hydraulic and overcoring stress data. For the latter case, the elastic parameters of the overcoring technique may be solved in situ by allowing the hydraulic stress data to constrain them. As a result, the problem then involves 14 model parameters. Results from the study show that the ISDM effectively improves the precision of the prevailing stress field determination and that it is especially powerful for identification of consistencies/inconsistencies in an existing data set. Indeed, this is the very basic premise and goal of stress integration; combine all available data to achieve as complete a characterization of the mechanical stress model as possible, and not to identify a solution that fits only loosely the maximum amount of stress data.  相似文献   

卡德藻自养、异养与兼养培养的比较研究   总被引：2，自引：0，他引：2  

刘晨临  王秀良  张学成 《中国海洋大学学报(自然科学版)》2002,32(4):579-584

本文对卡德藻在自养、异养和兼养培养条件下的比生长速率、细胞密度、细胞色素组成及脂肪酸组成等方面进行了比较研究。兼养卡德藻的细胞密度大于自养条件和异养条件下细胞密度之和。兼养培养的卡德藻比生长速率是自养的 2倍 ,异养的 1.3倍。兼养和异养生长的对数期较自养的长。光合自养培养卡德藻最适光强为 10 0 0 0 lx,而兼养培养最适光强范围为 10 0 0～ 2 0 0 0 lx。在自养和兼养培养时亚油酸的含量很高 ,自养和兼养状态下分别为 16 .6 %和 17.4 9%。 EPA合成的量较低。异养藻的多不饱和脂肪酸的产量较自养藻和兼养藻要低。兼养藻细胞光合色素组成和含量与自养藻细胞的基本一致 ,但在异养条件下藻细胞色素组成发生明显改变。  相似文献   

南极青霉菌Penicillium sp.S-1-16摇瓶发酵条件的初步优化          下载免费PDF全文

叶科元 唐潮卢小玲  吴文惠刘小宇 《极地研究》2016,28(3):424-429

对一株具有细胞毒活性的南极青霉属真菌Penicillium sp.S-1-16进行发酵条件的初步优化。通过对基础发酵培养基、发酵温度及培养基配制用水的筛选,以次级代谢产物量为主要指标,同时参考生物量及细胞毒活性指标来进行初步优化,获得一个较优的摇瓶发酵条件:酵母提取粉0.3%,蛋白胨0.5%,麦芽糖0.3%,葡萄糖1%,蔗糖5%,自来水,初始p H=7.0,接种量15%,18℃,180 r·min-1发酵15 d。采用初步优化后的条件进行发酵,与初始发酵条件相比,其次级代谢产物量提高到568.75%,对A549肿瘤细胞的抑制作用增强至265%,并且对He La肿瘤细胞和SGC-7901肿瘤细胞的抑制率分别从0提高到48.19%和69.18%,效果显著,为获得菌株中活性次级代谢产物奠定了基础。  相似文献   

对硫磷对扁藻和杜氏藻膜脂的过氧化与脱酯化伤害   总被引：8，自引：0，他引：8  

唐学玺  李永祺  黄健 《海洋与湖沼》1999,30(3):295-299

于１９９４－１９９５年,运用急性急性实验及生化方法对扁藻和杜氏藻的对硫磷毒性效应进行研究。结果表明,对硫磷对扁藻生长的抑制作用明显强于杜氏藻,在对硫磷的胁迫下,两种微藻细胞的超氧阴离子自由基相对含量上升,膜透性增大,微粒体膜脂过氧化水平提高。同时,扁藻微粒体膜的磷脂减少,游离脂肪酸含量增加。  相似文献   

Das Verhalten des Mikroorganismus Metallogenium spec. in einer kontinuierlichen Kultur     

Andreas Herschel 《Limnologica》1999,29(2):195

A series of 7 growth experiments with Metallogenium sp. was conducted in a flow-through reactor (chemostat). The results demonstrate that Metallogenium sp. grows under simulated lake water conditions in the laboratory. Metallogenium reacted in the chemostat in the same way as a microorganism. The growth rates of the microorganism Metallogenium were determined. It can be concluded therefore that the maximal growth rate at a temperature of 15 °C was 0.7 d⁻¹.  相似文献