厚壳贻贝(Mytilus coruscus)养殖区沉积物微生物多样性及固碳潜力研究          下载免费PDF全文

吴紫琪  何语堂  羊妍珂  陶玉林  吴骏男  蒋振楠  严小军  廖智  刘雪珠  何建瑜 《海洋与湖沼》2023,54(2):502-513

海洋蓝碳是海洋碳汇研究的重要领域,厘清不同蓝碳生境中沉积物有机碳组分格局是当前研究的热点之一。为更好地理解此问题,现以近海厚壳贻贝养殖区这一特殊蓝碳生境为对象,解析沉积物中的碳氮组分格局;进一步通过关联沉积物微生物群落、结合卡尔文循环和还原三羧酸循环的关键基因相对丰度分析,评估厚壳贻贝养殖区沉积物的固碳潜力。结果表明,相较于非养殖区,厚壳贻贝养殖区沉积物惰性碳的累积较大,氮组分主要以氨氮形式存在;同时养殖区高微生物量碳和微生物量氮指示了其沉积物中碳周转较快,碳氮组分特征差异明显。沉积物微生物高通量测序结果显示养殖区沉积物微生物主要以Gamma变形菌纲和Delta变形菌纲为主,且微生物类群与颗粒有机碳、惰性碳等碳组分存在明显的相关性。与惰性碳存在明显正相关关系的硫微螺菌科(Thiomicrospiraceae)丰度在养殖区沉积物中显著高于非养殖区沉积物。贻贝养殖区沉积物包含cbbL在内的6种关键功能基因,固碳潜力明显。研究结果将为进一步探究蓝碳生境的有机碳来源和微生物固碳效率提供基础依据。  相似文献   

Comparative assessment of nitrogen fixation rate by ¹⁵N₂ tracer assays in the South China Sea     

Danyang Li  Minfang Zheng  Yusheng Qiu  Limin Lai  Nengwang Chen  Hongmei Jing  Run Zhang  Min Chen 《海洋学报(英文版)》2023,42(1):75-82

Nitrogen fixation is one of the most important sources of new nitrogen in the ocean and thus profoundly affects the nitrogen and carbon biogeochemical processes. The distribution, controlling factors, and flux of N₂ fixation in the global ocean remain uncertain, partly because of the lack of methodological uniformity. The 15N₂ tracer assay (the original bubble method → the 15N₂-enriched seawater method → the modified bubble method) is the mainstream method for field measurements of N₂ fixation rates (NFRs), among which the original bubble method is the most frequently used. However, accumulating evidence has suggested an underestimation of NFRs when using this method. To improve the availability of previous data, we compared NFRs measured by three 15N₂ tracer assays in the South China Sea. Our results indicate that the relationship between NFRs measured by the original bubble method and the 15N₂-enriched seawater method varies obviously with area and season, which may be influenced by incubation time, diazotrophic composition, and environmental factors. In comparison, the relationship between NFRs measured by the original bubble method and the modified bubble method is more stable, indicating that the N₂ fixation rates based on the original bubble methods may be underestimated by approximately 50%. Based on this result, we revised the flux of N₂ fixation in the South China Sea to 40 mmol/(m2·a). Our results improve the availability and comparability of literature NFR data in the South China Sea. The comparison of the 15N₂ tracer assay for NFRs measurements on a larger scale is urgently necessary over the global ocean for a more robust understanding of the role of N₂ fixation in the marine nitrogen cycle.  相似文献   

不同氮源对海洋微藻氮同位素分馏作用的影响   总被引：2，自引：2，他引：0  

俞志 明  Waser N A D  Harrison P J 《海洋与湖沼》2004,35(6):524-529

以海链藻(Thalassiosira pseudonana)为研究对象,分别考察了以硝酸盐、铵盐和尿素为氮源的氮同位素分馏作用。在建立相应理论模型基础上,分别计算出各个实验体系的ε值。结果表明,在藻类生长初期,δ^15N均较低,其δ^15N的积累主要发生在指数增长期,在稳定期达到最高,与氮源的初始δ^15N相同;不同氮源的氮同位素分馏作用也不相同,其中以铵盐最强,硝酸盐次之,尿素最弱。考虑到实际情况下氮化合物并非单一存在,作者还进一步考察了上述3种氮源混合后对其同位素分馏作用的影响,发现混合氮源体系的表观ε值介于单一氮源时的最大与最小ε值之间,该结果较好地解释了Montoya等(1991)在Chesapeak湾的现场实验结果。  相似文献   

基于“水文频率-水质”拟合曲线的河流水质变异与等级特征值分析方法     

韩豪杰  严星  赵娣  夏永秋  颜晓元 《湖泊科学》2023,35(3):863-873

时空特征分析对全面掌握水质变异规律具有重要意义,但现有的水质时空特征分析方法仍存在水质变异次序不分、水质变幅极值不清、水质评价特征值不明等不足。为更加清晰地探析水质时空特征信息,以秦淮河为研究对象,参考工程水文学经验频率法,建立“水文频率-水质”拟合曲线用于探索流域内高/低活动区不同时间段和丰/枯水期不同河段水质变异特征,并与传统的箱线图法进行对比。结果表明:与箱线图相比,“水文频率-水质”拟合曲线可量化关键水质评判点与特征值信息,使水质时空变异过程更为清晰。在时间上“水文频率-水质”拟合曲线的最佳形式为线性曲线,水质浓度一般不会发生突变;在空间上“水文频率-水质”拟合曲线的最佳形式为指数曲线,水质浓度有较大可能发生突增。各时间段高活动区氮磷浓度大于低活动区,各水体断面丰水期氮磷浓度低于枯水期。该方法分析过程简单方便,结果直观有序,能将水质信息以统计规律自动反映出来,在水质采样点、采样时间和采样频率典型时可作为优选方法用于河流水质时空特征研究。  相似文献   

华南热带水库丰水期浮游植物群落对氮、磷、铁加富的响应——以广东大沙河水库为例     

谢静  苏宇亮  吴斌  张依  肖利娟  顾继光 《湖泊科学》2024,36(1):43-51

富营养化是水生态系统主要的环境问题,自然条件下多种营养盐以组合形式进入水体,明确浮游植物群落对多种营养盐加富的响应对富营养化防治有重要意义。本研究以一座华南热带水库(大沙河水库)为例,分析了2021年枯水期(1—3月)和丰水期前期(4—6月)氮(N)、磷(P)、铁(Fe)和叶绿素a(Chl.a)浓度的变化趋势,并通过原位添加实验,解析浮游植物丰度和多样性对N、P、Fe加富的响应。结果表明:与1—3月相比,4—6月期间水体总磷浓度(TP)明显升高,总铁浓度(TFe)呈增加趋势但月度间波动较大,总氮浓度(TN)在4月最低,之后缓慢增加,期间Chl.a浓度从3月的5 μg/L快速增加到5月的29 μg/L。原位实验结果表明:浮游植物群落以蓝藻、绿藻和硅藻为主要组成,所有营养盐添加组浮游植物丰度均有增加,但只有NP同时添加时(NP和FeNP两组)生物量(以Chl.a表征)有显著升高;营养盐添加组细胞丰度的响应在门类和种类间差异明显,实验组组间群落结构和优势种类差异显著,所有处理组的群落多样性均明显高于对照组。N和P添加及其与Fe的联合添加(N、P、NP、FeN、FeP和FeNP)均导致蓝藻丰度升高,绿藻仅在NP添加组(FeNP组和NP组)显著升高且两组没有显著差异,硅藻仅在N和P单独添加组(N和P)显著升高。上述结果说明浮游植物生物量受N、P营养盐的共同影响而非单因子作用,N、P和Fe添加均影响群落结构和多样性,对水库富营养化的管理尤其是蓝藻水华防治应综合考虑3种营养盐的共同作用。  相似文献   

The degree of urbanization across the globe is not reflected in the δN of seagrass leaves     

Bart Christiaen  Rebecca J. Bernard  Behzad Mortazavi  Just Cebrian  Alice C. Ortmann 《Marine pollution bulletin》2014

Many studies show that seagrass δ¹⁵N ratios increase with the amount of urbanization in coastal watersheds. However, there is little information on the relationship between urbanization and seagrass δ¹⁵N ratios on a global scale. We performed a meta-analysis on seagrass samples from 79 independent locations to test if seagrass δ¹⁵N ratios correlate with patterns of population density and fertilizer use within a radius of 10–200 km around the sample locations. Our results show that seagrass δ¹⁵N ratios are more influenced by intergeneric and latitudinal differences than the degree of urbanization or the amount of fertilizer used in nearby watersheds. The positive correlation between seagrass δ¹⁵N ratios and latitude hints at an underlying pattern in discrimination or a latitudinal gradient in the ¹⁵N isotopic signature of nitrogen assimilated by the plants. The actual mechanisms responsible for the correlation between δ¹⁵N and latitude remain unknown.  相似文献   

δ15N variation in Ulva lactuca as a proxy for anthropogenic nitrogen inputs in coastal areas of Gulf of Gaeta (Mediterranean Sea)     

《Marine pollution bulletin》2014,85(1-2):76-82

We tested the capacity of Ulva lactuca to mark N sources across large marine areas by measuring variation in its δ¹⁵N at several sites in the Gulf of Gaeta. Comparisons were made with the macroalga Cystoseira amentacea. Variation of δ¹⁵N values was assessed also in the coastal waters off the Circeo Natural Park, where U. lactuca and C. amentacea were harvested, as these waters are barely influenced by human activities and were used as reference site. A small fragment from each frond was preserved before deployment in order to characterize the initial isotopic values. After 48 h of submersion, U. lactuca was more responsive than C. amentacea to environmental variation and δ¹⁵N enrichment in the Gulf of Gaeta was observed. The spatial distribution of δ¹⁵N enrichment indicated that different macro-areas in the Gulf were affected by N inputs from different origins. Comparison of the δ¹⁵N values of fragments taken from the same transplanted frond avoided bias arising from natural isotopic variability.  相似文献   

Anthropogenic land-use signals propagate through stream food webs in a California,USA, watershed     

《Limnologica》2014

Human development of watersheds can change aquatic ecosystems via multiple pathways. For instance, human rural development may add nutrients to ecosystems. We used naturally occurring stable isotopes in stream food webs to investigate how land use affects stream ecosystems across a gradient of land development in the San Lorenzo watershed, California. Road density was used as a proxy for land development. We found that streams in watersheds with higher road densities had elevated concentrations of phosphate and nitrate. Furthermore, algal δ¹⁵N values increased as a function of nitrate concentration, but saturated at approximately 6‰. This saturating pattern was consistent with a two-source mixing model with anthropogenic and watershed sources, fit using Bayesian model fitting. In sites that had >2.6 km roads km⁻², anthropogenic sources of N were estimated to represent >90% of the N pool. This anthropogenic N signal was propagated to stream consumers: rainbow trout (Oncorhynchus mykiss), signal crayfish (Pacifasticus leniusculus), and benthic invertebrate δ¹⁵N were positively correlated with algal δ¹⁵N. Even relatively low density rural human land use may have substantial impacts on nutrient cycling of stream ecosystems.  相似文献   

Atmospheric Nitrogen and Phosphorus Deposition at Three Sites in Nanjing,China, and Possible Links to Nitrogen Deposition Sources   总被引：1，自引：0，他引：1       下载免费PDF全文

Liying Sun  Yinglie Liu  Jinyang Wang  Mohammad Aslam K. Khalil  Jianwen Zou  Zhengqin Xiong 《洁净——土壤、空气、水》2014,42(11):1650-1659

  相似文献   

哈尔滨-绥化地区土壤氮储量及其时空变化特征     

张立  崔玉军  刘国栋  李瑛  孙振伟 《地质与资源》2014,23(2):188-191

依据多目标区域地球化学调查成果,按照中国地质调查局下发的《全国土壤碳储量及各类元素（氧化物）储量实测计算暂行要求》,以表层土壤样品分析单元（4 km²）为计算单位,对哈尔滨-绥化地区表层土壤（0~20 cm）氮储量进行计算.研究了不同生态系统和不同土壤类型土壤氮储量、氮密度的差异,研究了20年来土壤氮储量的变化特征及成因.结果表明研究区土壤氮储量略微增加,这主要是由于湿地生态系统和森林生态系统对土壤的固氮效果显著造成的.  相似文献