大菱鲆红体病虹彩病毒体外增殖条件的研究     

樊廷俊  王丽燕  耿晓芬  丛日山  李明玉  于秋涛  杨秀霞 《中国海洋大学学报(自然科学版)》2006,36(5):767-774

探讨大菱鲆病毒疫苗的研制,以牙鲆鳃细胞(FG细胞)为增殖体系,利用细胞病变效应(CPE)为判断指标,对大菱鲆红体病虹彩病毒(Turbot reddish body iridovirus,TRBIV)的体外最适增殖条件等进行了研究。结果表明,病毒接种量、病毒吸附时间、细胞培养条件对TRBIV的体外增殖均有明显的影响,以0.2 mL病毒液接种FG细胞、吸附2 h、用含10%胎牛血清的MEM培养液于22℃培养时,病毒的增殖速度最快。研究还发现,病毒的收获时间和病毒液冻融次数对所得病毒的感染力均有显著影响,在最佳增殖条件下,病毒在FG细胞中增殖至第4天时进行收获,且只冻融1次,其感染活性最强,反复冻融后病毒的感染活性会显著降低。  相似文献   

Phosphate Utilization in Phaeocystis pouchetii (Haptophyceae)     

M.J. W. Veldhuis    F. Colijn  W. Admiraal 《Marine Ecology》1991,12(1):53-62

Abstract. .The phosphate uptake kinetics of single cells and colonies of the haptophycean alga Phaeocystis pouchetii were investigated in short- and long-term experiments. In phosphate-deprived cultures, the highest nutrient uptake rate (V_max) of both growth forms appeared to be similar. However, the half-saturation constant for phosphate uptake of the cells embedded in a colony (K_s= 3.08 μM) was considerably higher than that of the single cells (K_s= 0.31 μM). This is consistent with the existence of a diffusion barrier formed by the colony membrane and colonial mucus. Over a 24 h light/dark cycle, the phosphate assimilation rate in moderately limited colonies and single cells was nearly identical during the light period. In the following dark phase, colony cells maintained this uptake rate, whereas the uptake of the single cells diminished by 70 %. The high dark uptake rate in colonies ran parallel with the absorption and degradation of intracolonial carbon compounds, suggesting that the dissimilation of these compounds covers the energy requirements for phosphate uptake in the dark. In Phaeocystis cultures during a transition to phosphate depletion, a significant amount of phosphate was found in the colonial mucus, isolated by selective filtration. The possible absorption of phosphate by the mucus and the role of mucus in the energy budget seem to be an essential feature of phosphate uptake of colonies. Short-term measurement of phosphate uptake in colony cells may therefore underestimate the phosphate uptake of the whole colony.  相似文献   

一例长生命史雷暴云分裂过程的回波特征   总被引：9，自引：8，他引：1  

张鸿发  左洪超  陈成品  郭三刚 《高原气象》2004,23(1):40-47

在对平凉冰雹云演变规律研究中,发现一例长生命史强雷暴云分裂成两个不同移动方向的单体雹云。结合探空资料和环境风场,分析了此个例强雷暴云分裂过程的回波特征。结果表明,在强风切变环境中,u与v分量呈反相关,且随高度增强,低层1～3km为弱气旋旋转,中层3～8km为强反气旋旋转,上层8km以上是强气旋旋转。这对右移雷暴云发展中有优势引导作用,导致强雷暴云上层偏向分离发展,分裂发生在强雹暴云有强下沉气流阶段。分裂后,左、右移单体各自维持发展,并产生降雹。整个雷暴发展演变过程维持长生命期,回波特征有四个不同阶段。  相似文献   

浙江枝吻纽虫(Dendrorhynchus zhejiangensis)凝溶胶蛋白基因的原核表达产物抗肿瘤活性的研究     

李晔  陈蕾  李振  苏秀榕  周君  李太武  江锦坡 《海洋与湖沼》2012,43(4):756-760

凝溶胶蛋白(Gelsolin)作为一种重要的肌动蛋白调节蛋白,在机体的多种生理和病理活动中发挥重要作用。本文采用细胞MTT染色法,Hoechst33342/PⅠ荧光染色和流式细胞仪技术,对浙江枝吻纽虫(Dendrorhynchus zhejiangensis)的gelsolin基因的原核表达产物(r-gelsolin)进行了体外抗肿瘤活性的研究。结果表明,30μg/ml的r-gelsolin作用于胃低分化粘液腺癌MGC803细胞24h后,细胞的增殖抑制率达到最高,约为34.53%。荧光染色显示,r-gelsolin使MGC803细胞的正常形态发生改变,细胞核出现核固缩,破裂,产生凋亡小体等现象。进一步通过流式细胞仪证实,r-gelsolin可以阻滞MGC803细胞于S、G2期,影响细胞分裂的进程,并促使细胞发生凋亡。  相似文献   

Hydrothermally induced diagenesis: Evidence from shallow marine-deltaic sediments,Wilhelmøya,Svalbard     

Beyene G.Haile  Urszula Czarniecka  Kelai Xi  Aleksandra Smyrak-Sikora  Jens Jahren  Alvar Braathen  Helge Hellevang 《地学前缘(英文版)》2019,10(2):629-649

Sedimentary basins containing igneous intrusions within sedimentary reservoir units represent an important risk in petroleum exploration. The Upper Triassic to Lower Jurassic sediments at Wilhelm?ya(Svalbard) contains reservoir heterogeneity as a result of sill emplacement and represent a unique case study to better understand the effect of magmatic intrusions on the general burial diagenesis of siliciclastic sediments. Sills develop contact metamorphic aureoles by conduction as presented in many earlier studies. However, there is significant impact of localized hydrothermal circulation systems affecting reservoir sediments at considerable distance from the sill intrusions. Dolerite sill intrusions in the studied area are of limited vertical extent(～12 m thick), but created localized hydrothermal convection cells affecting sediments at considerable distance(more than five times the thickness of the sill)from the intrusions. We present evidence that the sedimentary sequence can be divided into two units:(1) the bulk poorly lithified sediment with a maximum burial temperature much lower than 60-70 ℃,and(2) thinner intervals outside the contact zone that have experienced hydrothermal temperatures(around 140 ℃). The main diagenetic alteration associated with normal burial diagenesis is minor mechanical plastic deformation of ductile grains such as mica. Mineral grain contacts show no evidence of pressure dissolution and the vitrinite reflectance suggests a maximum temperature of ～40 ℃. Contrary to this, part of the sediment, preferentially along calcite cemented flow baffles, show evidence of hydrothermal alteration. These hydrothermally altered sediment sections are characterized by recrystallized carbonate cemented intervals. Further, the hydrothermal solutions have resulted in localized sericitization(illitization) of feldspars, albitization of both K-feldspar and plagioclase and the formation of fibrous illite nucleated on kaolinite. These observations suggest hydrothermal alteration at T 120-140 ℃ at distances considerably further away than expected from sill heat dissipation by conduction only, which commonly affect sediments about twice the thickness of the sill intrusion. We propose that carbonate-cemented sections acted as flow baffles already during the hydrothermal fluid mobility and controlled the migration pathways of the buoyant hot fluids. Significant hydrothermally induced diagenetic alterations affecting the porosity and hence reservoir quality was not noted in the noncarbonate-cemented reservoir intervals.  相似文献   

我国温石棉与其4种代用品致V79细胞肿瘤蛋白谱表达的影响     

曾娅莉  王洪州  董发勤  王利民  邓建军 《岩石矿物学杂志》2013,32(6):803-808

将中国仓鼠肺细胞(Chinese Hamster Lung Cell, V79细胞)分别暴露于不同浓度的温石棉及代用品粉体悬液中48 h后,MTT检测细胞存活率;免疫组化法检测V79细胞中Survivin、Cap43、Bcl-2、p16和p53肿瘤相关蛋白的分布及表达,分析我国两大主产区的温石棉及4种主要人工代用品对V79细胞的增殖抑制率与肿瘤相关蛋白谱的影响,以探讨我国温石棉及人工代用品是否是导致肿瘤的高危因素之一。结果表明: ① 在6种粉体中,四川新康及陕西陕南温石棉对细胞生长抑制最强,岩棉纤维粉体对细胞生长抑制最弱。各粉体对V79细胞的存活率影响不同,随着暴露粉体浓度的增加,细胞的存活率降低,呈现剂量效应关系。②各染毒组细胞的癌基因Survivin、Cap43、Bcl-2表达的蛋白上调,抑癌基因p16和p53表达的蛋白下调,以胞浆阳性为主。综合以上结果,发现我国不同地区温石棉及其代用品均不同程度抑制V79细胞生长,上调肿瘤相关蛋白Survivin、Cap43及Bcl-2,下调p16和p53,从而诱导癌症的发展。  相似文献   

海洋无脊椎动物细胞培养与细胞干性的捕获     

岑卫  胡梦珠  Rinkevich Baruch  胡国斌 《海洋科学》2021,45(1):146-154

细胞系的构建在生物学领域具有重要意义,与其他动物(如昆虫)不同,经过数十年的发展,海洋无脊椎动物细胞研究仍停留在原代培养水平,没有建立起克隆化的永生细胞系.本文主要介绍了近年来海洋无脊椎动物细胞培养的研究进展,还介绍了一个基本原理,即海洋无脊椎动物永生细胞系的建立,可以通过捕获细胞的干性而实现.捕获细胞干性的第一条途径...  相似文献   

LED光谱对许氏平鲉视网膜形态的影响          下载免费PDF全文

吴燕玲  孙飞  蔡皓玮  李鑫  刘鹰  刘松涛  马贺 《海洋科学》2023,47(9):71-80

光作为一个复杂的环境因子,对鱼类的存活、摄食、生长及视觉均产重要影响。为了探究LED光谱对许氏平鲉(Sebastods schlegelii)视网膜结构和视网膜各层细胞数目与厚度的影响,本文选取1 000尾许氏平鲉幼鱼(体质量为38.80±0.43 g、体长为10.20±0.17 cm)分别养殖在5种光谱下(蓝光(λ450 nm)、绿光(λ525 nm)、黄光(λ590 nm)、白光(λ460 nm)、红光(λ630 nm)),其中白光组为对照组,其余4组为处理组。经过为期60 d的处理后,分别对各组的视网膜进行取样观察。结果表明:不同的LED光谱对许氏平鲉的视网膜结构未产生影响,但各个光谱下视网膜细胞数目略有差别,在5种不同光谱条件下,在单位面积(100μm×100μm)内,蓝光组内网层细胞数量(24.33±2.08)显著低于白光组(P<0.05);红光组外核层细胞数量(33.33±3.51)显著低于除白光组外的其他各组(P<0.05),通过对比各个光谱下视网膜各层细胞数量可得,蓝光组与红光组数量最少,其他各层差异不大。综上所述,饲养于不同光谱处理组的许氏平鲉幼鱼的视网膜...  相似文献   

京藏高速民和至西宁段冬季路面与地面最低温度变化特征及影响分析          下载免费PDF全文

保广裕  张静  刘玮  雷春苗  郑玲  李宝华 《沙漠与绿洲气象(新疆气象)》2022,16(5):94-101

利用2018年1月1日至2020年12月冬季1月、2月和12月-次年2月逐日逐小时京藏高速民和至西宁段常规气象站和交通气象站逐日逐时气象观测资料,分析了冬季逐月平均路面最低温度和地面最低气温的日变化特征及其相关关系,建立了4站冬季各月路面最高温度和最低温度分别与地面最高温度和最低温度的相关性方程,旨在为路面温度精细化预报服务提供参考。结果表明：京藏高速民和至西宁路段4站冬季各月平均路面最低温度和地面最低温度冬季各月具有明显的日变化特征,平均路面最低温度和地面最低温度达到最低值和最高值的时间并不是完全相同；。常规气象站点平均地面最低温度日出/日落的变化速率要高于交通站点平均路面最低温度,并且平均地面最低温度的变化幅度要比路面最低温度变化幅度大。平均地温面最低温度的最高值比路面最低温度的最高值超前1～2h,常规气象站点逐小时地面最低温度＜0℃的时间维持15～18h,交通站点路面最低温度＜0℃的时间维持8～22h。应用统计学方法建立的朝阳站、汉庄站、高庙桥站和老鸦峡站最高和最低路面温度与最高和最低地面温度相关性方程具有很好的实际应用价值,可在实际业务工作中推广应用。  相似文献