不同浓度亚油酸对日本对虾肝胰腺传代细胞磷含量的影响     

王宏伟  王安利  王维娜  刘瑞兰  候雅宾 《海洋科学》2003,27(9):78-80

在199培养基中加入促进细胞生长的亚油酸 ,对日本对虾(Penaeusjaponicus)肝胰腺细胞进行传代培养,测定传代细胞的总磷、无机磷含量。当亚油酸的加入量为16×10 -5mol/L时,细胞的总磷、有机磷含量均为最高。表明培养基中加入适量亚油酸 ,可促进日本对虾肝胰腺传代细胞的生长  相似文献   

中国对虾血淋巴中的抗菌、溶菌活力与酚氧化酶活力的测定及其特性研究   总被引：73，自引：8，他引：73  

王雷  李光友  毛远兴 《海洋与湖沼》1995,26(2):79-185

以海捕中国对虾亲虾（1992年4月捕）为材料，对其血淋巴中的抗菌、溶菌活力及酚氧化酶活力进行测定，并研究经注射大肠杆菌、海洋弧菌及酵母聚糖等刺激物以后，其活性的变化规律等。结果表明，适当的免疫刺激可提高对虾自身的抗病能力、为免疫药物的研制等提供科学依据。  相似文献   

The Complete Sequence of Mitochondrial COⅡ Gene of Fenneropenaeus chinensis and Its Applicability as a Marker for Phylogenetic Analysis     

YU Shanshan  KONG Xiaoyu  LI Yulong  XU Hui 《中国海洋大学学报(英文版)》2007,6(2)

The complete mitochondrial cytochrome oxidase subunit Ⅱ (COⅡ) gene of Penaeinae shrimp Fenneropenaeus chinensis was cloned and sequenced. The gene is 688 bp in length and codes for 229 amino acids. It shows 83.2%, 87.0% and 83.8% sequence similarity to Marsupenaeus Japonicus, Penaeus monodon and Farfantepenaeus notialis, respectively. The A+T content of the whole gene and that at the third position of codons are 64.7% and 78.2%, respectively. The phylogenetic relationship between F. chinensis and three other species representing genera Farfanatepenaeus, Marsupenaeus and Penaeus was analyzed. Results showed that the genetic distances among the four taxa ranged from 0.144 0 to 0.200 5, exceeding those estimated with COⅠ and partial 16S rRNA gene sequences among Marsupenaeus, Litopenaeus and Melicertus, and being therefore larger than the value among subgenera. It has been suggested that the COⅡ gene has a faster evolutionary rate than that of the COⅠ gene and partial 16S rRNA gene and could be used for phylogenetic analysis at genus or species level. The results of the present study indicated that Farfantepenaeus, Fenneropenaeus, Marsupenaeus and Penaeus are at a higher phylogenetic level than subgenus, which supports the opinion of the elevation of phylogenetic status of the four subgenera to genus level.  相似文献   

中国蛤蜊Mactra chinensis Philippi稚贝运动行为学初步研究          下载免费PDF全文

李金泽  胡志  王晓龙  李永仁  郭永军  张涛 《海洋科学》2023,47(12):63-68

为从行为学角度解释中国蛤蜊(Mactra chinensis Philippi)的跑滩现象, 研究了规格、温度对其跳跃行为以及底质、流速和周期性干露对其潜沙行为的影响。结果表明, 中国蛤蜊运动能力与其规格和水温有关, 3 mm稚贝不具备跳跃能力, 5 mm以上具备跳跃能力; 跳跃频率和高度随水温的升高呈上升趋势。中国蛤蜊稚贝潜沙比例均随时间推移逐渐上升; 各时间节点中国蛤蜊稚贝在细沙底质的潜沙比例最高, 中沙底质次之, 粗沙底质最低。施加微弱水流后, 中国蛤蜊稚贝潜沙速度和比例显著增加; 流速增大到一定程度后部分稚贝被水流冲走, 潜沙比例随之下降。周期性干露会导致中国蛤蜊稚贝从底质中爬出, 干露时间越长, 爬出比例越高。干露后的稚贝重新放置于海水中, 部分稚贝会在水面漂浮一段时间后再下落至水底, 漂浮比例随干露时间的增加而增加。本研究从行为学角度初步查明了中国蛤蜊跑滩现象, 为解决中国蛤蜊防跑滩问题提供了思路。  相似文献   

答《关于深海锰结核中“纳米级超微生物化石”的思考和建议》一文   总被引：2，自引：0，他引：2  

林承毅  边立曾 《高校地质学报》1997,3(2):236-240

本文详细讨论了东太平洋深海锰矿结核中新发现的中华微放线菌和太平洋螺球孢菌究竟是不是超微结构，首先，它们是笔者根据形态，结构和元素成分作为超微生物化石进行鉴定的，其次，透射电子显微镜中可以清楚地区分出完整的微生物结构，包括菌丝，孢子，孢囊和菌落等，它们之间的尺寸比例也是适当的，此外，透射电子显微像还揭示，深海锰质叠层石的纹层就是这些超微生物韵律性生长的结果，与滨海叠层中的纹层十分相似，笔者认为，已知微生物的最小尺寸不能作为否定新发现的超微生物化石的依据。本文还讨论了超微生物化石的术语问题。  相似文献   

黄连种植对生态环境影响的初步研究   总被引：4，自引：0，他引：4  

陈桂芳  冉成 《云南地理环境研究》2003,15(4):61-65

黄连对生境要求特殊，其生产完全依赖于森林资源的消耗，每种1hm^2黄连要破坏3hm^2林地，消耗150m^3木材，每个种植阶段都会引起较严重的水土流失，其中第一年的土壤侵蚀模数为5360t／km^2。在重庆市石柱县黄连主产区黄水镇，由于历年来一直种植黄连，野生动物生存环境长期受到破坏，沟河鱼类资源2001年比1981年减少。73％，有8种大型陆生脊椎动物绝迹，而一些食草性动物的数量近年有所增加。  相似文献   

STUDIES ON IMMUNODIAGNOSIS OFHEPATOPANCREATIC PARVO- LIKEVIRUS DISEASE OF THE CHINESEPENAEID, PENAEUS CHINENSISI. PURIFICATION OF THE VIRUS     

孙修勤  王文兴  周惠民  张进兴  薛清刚  卢颖  宋庆云 《中国海洋湖沼学报》1993,(2)

The viral disease of penaeids is indistinct but highly dangerous because their symptoms are often masked by some secondary ones.In order to gain knowledge on how to prevent the occurrence and spread of this viral disease, diagnostic studies on the early phase of the hepatopancreatic parvo-like viral (HPV)disease of cultured Penaeus chinensis was conducted using immuno-serological techniques. The purification of HPV was successfully done by density gradient ultracentrifugation of cane sugar.  相似文献   

Selection of better extractants to indicate Pb bioavailability in lead-contaminated agricultural soils of Zhejiang Province     

ZENG Lusheng  LIAO Min CHEN Chengli HUANG Changyong 《中国地球化学学报》2007,26(4):407-413

The accumulation of Pb in the food chain is one of the great concerns as it can cause chronic health problems and the available Pb is easily absorbed by crops. To shed light on Pb bioavailability in lead-contaminated agricultural soils of Zhejiang Province, a series of plant growth experiments were performed in a greenhouse to select more suitable extractants from five commonly used extractants (0.1 M HCl, 1 M NH4OAc, 0.1 M CaCl2, DTPA-TEA and Mehlich 3). The results showed that 1 M NH4OAc can extract Pb best, indicating the Pb bioavailability, then, DTPA-TEA and 0.1 M HCl in the tested soils. In case 1 M NH4OAc was used as an extractant, the critical Pb concentrations in soils were 50.19 mg/kg, 21.16 mg/kg and 114.00 mg/kg (1 M NH4OAc extracted Pb) in silty loam, yellowish-red soil and purplish soil, respectively. When the values exceed the above ones, the contents of Pb in Chinese cabbage leaves will exceed the Chinese Tolerance Limit of Lead in Foods (GB14935-94), as a result, potential ecological risk and hazard to human health via the food chain will appear.  相似文献   

Distribution of Bacteria Injected in Body of Giant Black Shrimp, Penaeus Monodon     

郭志勋  Karin van de Braak  Magriet Botterbloom 《中国海洋湖沼学报》2004,22(2)

1　INTRODUCTIONThepenaeidshrimpcultureindustriesintheworlddevelopedfromtheirexperimentalbeginningslessthanthirtyyearsagointoamajoraquaculturein dustrytodaythatprovideshundredsofthousandsofjobs,billionsofU .S.dollarsinrevenue,andaug mentstheglobalfoodsupplywithahighvaluecrop(LighterandRedmam ,1 998) .Upto 1 995,farmedshrimpatvolumeof71 2 0 0 0MT ,almostallconsistingofPenaeidspecies,accountedfor 2 7%ofworldshrimpproduction.Inthatyear,asoneofthemostimportantculturedshrimp,theproductionofP…  相似文献   

干旱和复水对羊草碳氮分配的影响   总被引：1，自引：0，他引：1  

许振柱  周广胜  王玉辉 《气象与环境学报》2007,23(3):65-71

植物的碳氮营养及其相互关系是最重要的基本生物过程之一,阶段性干旱对植物碳氮分配的影响研究甚少。实验以中国北方草原的典型植物羊草为材料,研究不同干旱持续期复水对羊草碳氮含量、分配及其相互关系的影响。结果表明:短期和中期干旱使植株生物量、氮素水平和单株总氮量增加,但长期干旱使之降低。水分处理对碳含量的影响不显著。干旱后复水降低了各器官特别是绿叶的碳氮比。中度持续干旱的氮素利用率(NUE)最高、短期干旱最低。羊草各器官氮素绝对量占整株的百分比从大到小依次为:绿叶、根茎、根、枯叶和茎鞘,其中叶片的氮素总量占植株的一半以上;随着干旱持续期的增加,氮素对根部的投资亦加大。羊草受到适当干旱驯化后复水引发的超补偿作用可促进羊草植株生长、提高氮素水平,并在一定程度上通过碳氮分配的调节作用来适应于阶段性的干旱胁迫。  相似文献