深海潜标ADCP 的实时数据传输   总被引：2，自引：2，他引：0  

赵忠生  袁志伟  黄磊  李延刚  韩雪双  杨宝起  陈海涛 《海洋科学》2012,36(8):94-97

深海潜标观测是深海观测获取长周期海洋科学数据的重要调查手段,由于深海海域环境非常复杂,潜标易丢失或终止正常工作,造成重大损失.本研究基于铱星卫星数据通信模块,开发与深水潜标上安装的RDt 75k ADCP相匹配的数据解析压缩软硬件,建立一套远程实时获取潜标ADCP数据的传输系统,实现对深海潜标ADCP的实时监测.  相似文献   

创伤弧菌和哈氏弧菌双重PCR检测方法的建立及初步应用     

陈福艳  程光平  欧阳贤华  陈晓汉  梁万文  韦友传  黄婷  杨学明  陈明  王瑞  李丽萍  韦明利 《广东海洋大学学报》2013,(3)

根据创伤弧菌(Vibrio vulnificus)的溶细胞毒素基因序列和哈氏弧菌(Vibrio harveyi)的toxR基因序列,分别设计并合成两对特异性引物,通过PCR反应条件优化,测试两种菌的特异性和敏感性,建立双重PCR方法,同时快速检测V.vulnificus和V.harveyi。结果表明:纯培养V.vulnificus和V.harveyi的检测灵敏度分别是12 cfu/mL和18 cfu/mL,与无乳链球菌、海豚链球菌、副溶血弧菌及美人发光杆菌无交叉反应;此PCR检测方法具有良好的特异性、敏感性,具快速、高效等优点,对细菌V.vulnificus和V.harveyi诊断与防治具有较好的临床应用性。  相似文献   

新疆泥火山群地震前兆异常实时监测与预报的研究   总被引：1，自引：0，他引：1  

高小其  王海涛  郑黎明  李娜  朱成英  杨晓芳  汪成国  向阳  梁卉  麻荣 《震灾防御技术》2015,10(3):587-597

基于网络技术的视频监控服务,实现了对新疆北天山地区3个泥火山点的实时监测,可在线实时查看泥火山活动情况,分析预报人员依据泥火山活动图像可开展地震预测研究。新疆艾其沟泥火山网络视频监控服务系统扑捉到了2次6级地震前火山液面明显的宏观异常变化现象,这说明基于宽带网络技术的网络视频监控服务,可实现互联网用户使用客户端远程软件连接服务器,实现在线实时查看泥火山活动情况的监控画面,并依据泥火山群地震观测网,捕捉泥火山群地震前兆异常。  相似文献   

土-结构动力相互作用的振动台试验研究综述   总被引：1，自引：1，他引：0  

李振宝  李晓亮  唐贞云  纪金豹 《震灾防御技术》2010,5(4):439-450

本文从土-结构动力相互作用振动台试验过程中所涉及的结构模型动力相似设计、模型土体的模拟及土体边界条件的模拟三个方面,回顾了近几年来土-结构动力相互作用振动台试验研究的发展历程与现状,重点描述了试验过程中为了更好地反映震动条件下土与结构动力相互作用的机理,学者们所采取的办法和措施。并在此基础上,介绍了一种新的研究土-结构动力相互作用的振动台试验技术。最后,总结了传统的土箱-振动台试验存在的不足,并与这种新的试验技术进行了对比,提出了对于这种新的试验方法仍需要进一步研究和解决的问题。  相似文献   

考虑土-结构相互作用的振动台实时子结构试验仿真分析     

周大兴  闫维明  陈彦江  何浩祥 《震灾防御技术》2010,5(1):27-31

本文基于状态空间方程进行了实时子结构试验的初步探索,提出了一种新的实时子结构试验方法。通过simulink仿真发现,这种方法能很好地再现整体分析的结构反应。最后,对考虑土-结构相互作用的振动台实时子结构试验进行了仿真分析。  相似文献   

Coupling the k-nearest neighbor procedure with the Kalman filter for real-time updating of the hydraulic model in flood forecasting   总被引：2，自引：0，他引：2  

《国际泥沙研究》2016,(2):149-158

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Potential use of dissolved cyanobacterial DNA for monitoring toxic Microcystis cyanobacteria in filtered water     

《Physics and Chemistry of the Earth》2013

Toxic and non-toxic Microcystis sp. are morphologically indistinguishable cyanobacteria that are increasingly posing health problems in fresh water systems by producing odours and/or toxins. Toxic Microcystis sp. produces toxicologically stable water soluble toxic compounds called microcystins (MCs) that have been associated with cases of aquatic life and wildlife poisoning and kills including some cases of human illnesses/deaths around the world. Thus, the need for rapid detection of toxic Microcystis sp. in surface water is imperatively a necessity for early mitigation purposes. Genomic DNA from potentially toxic Microcystis sp. comprises of ten microcystin synthetase (mcy) genes of which six major ones are directly involved in MCs biosynthesis. In Polymerase Chain Reaction (PCR) methodsmcy genes can be amplified from intracellular/extracellular genomic DNA using PCR primers. However, little is known about the limitations of sourcing genomic DNA templates from extracellular DNA dissolved in water. In this work, filtered water (0.45 μM) from a Microcystis infested Dam (South Africa) was re-filtered on 0.22 μM syringe filters followed by genomic DNA isolation and purification from micro-filtrates (9 mL). Six major mcy genes (mcyABCDEG) from the isolated DNA were amplified using newly designed as well as existing primers identified from literature. PCR products were separated by gel electrophoresis and visualized after staining with ethidium bromide. The limitation of using dissolved DNA for amplification of mcy genes was qualitatively studied by establishing the relationship between input DNA concentrations (10.0–0.001 ng/μL) and the formation of respective PCR products. The amplification of mcyA gene using new primers with as little as 0.001 ng/μL of DNA was possible. Other mcy gene sensitivities reached 0.1 ng/μL DNA dilution limits. These results demonstrated that with appropriately optimized PCR conditions the method can provide accurate cost-effective tools for rapid detection of toxic Microcystis sp. in water giving early information for water quality monitoring against MC producing cyanobacteria.  相似文献   

Reliable Procedure for Molecular Analysis of Airborne Microflora in Three Indoor Environments: An Office and Two Different Museum Contexts     

Carole Gaüzère  Marina Moletta‐Denat  Faisl Bousta  Stéphane Moularat  Geneviève Orial  Sébastien Ritoux  Jean‐Jacques Godon  Enric Robine 《洁净——土壤、空气、水》2013,41(3):226-234

Biological aerosols from air constitute a significant source of exposure to microorganisms in public places. Airborne microorganisms are involved in the development of certain respiratory symptoms, allergies, or infections among users and occupants. Various sampling instruments have commonly been used in aerobiology to collect bacteria and fungi suspended in the air. The objective of this study was to develop a reliable procedure for sampling in indoor public environments presenting different levels of occupancy, airborne bacteria and fungi to be subjected to molecular analysis (bacteria and fungi quantitative PCR, capillary electrophoresis single strand conformation polymorphism fingerprinting). Four different sampling devices were tested in situ in an office building (open‐plan type) and the sampling strategy chosen was tested in two museum contexts. In accordance with the drawbacks involved to our study (quantitative and qualitative aspects, cost, and overcrowding), cyclone device appeared to be most suitable. The results underline the effectiveness of this high‐volume aerosol sampling device for both qualitative and quantitative molecular analysis. Four in situ sampling collections were carried out in 1 day in the Louvre Museum to study quantitative and qualitative variations of airborne bacterial and fungal diversity. The quantitative results revealed a similar order of magnitude for the numbers of both bacteria and fungi. In the Louvre Museum, the samples yielded between 3.7 × 10⁴ and 4.1 × 10⁴ genome equivalent (GE) bacteria/m³ air and between 5.0 × 10⁴ and 5.9 × 10⁴ GE fungi/m³ air and in the Decorative Arts Museum between, 2.1 × 10⁴ and 2.5 × 10⁴ GE bacteria/m³ air and between 1.4 × 10⁴ and 1.7 × 10⁴ GE fungi/m³ air. The results also indicate that the dominant bacterial community displayed a stable structure over a short period of time whereas dominant eukaryotic airborne community appeared more variable.  相似文献   

实时精密单点定位精度与收敛性分析     

任晓东  何俊  张小红 《全球定位系统》2011,36(4):10-15

介绍了目前国际全球定位系统服务（IGS）组织提供的实时精密轨道和精密钟差改正系数（ROCC）的基本参数以及能够进行实时精密单点定位软件（BNC）（即BKG Ntrip Client,由BKG开发的一款用于实时同步接收、解码及转换的GNSS数据流管理软件）。选取了25个全球IGS跟踪站,并基于BNC软件分析了IGS提供的15种ROCC产品对测站实时精密单点定位精度与收敛性的影响。实验结果表明：采用BNC软件,15种ROCC产品均能在平均15min的时间收敛,并且在N、E方向达到6～8cm,U方向10～20cm的定位精度;且不同ROCC产品其收敛时间和定位精度都存在一定的差异。  相似文献   

CYP1A mRNA expression in redeye mullets (Liza haematocheila) from Bohai Bay, China     

An L  Hu J  Yang M  Zheng B  Wei A  Shang J  Zhao X 《Marine pollution bulletin》2011,62(4):718-725

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