不同氮源下南极冰藻Chlamydomonas sp.ICE-L的生物量、氮吸收与脂肪酸组成研究     

安美玲  王以斌  刘芳明  綦晓青  郑洲  叶乃好  孙承君  缪锦来 《海洋学报(英文版)》2017,36(2):105-110

微藻培养过程中氮缺失有利于油脂和生物量的积累,然而不同氮源条件下微藻生长与生物量的研究有限,限制了生物油脂的相关研究。本文研究通过研究南极冰藻Chlamydomonas sp.ICE-L在不同氮源条件下的细胞生长与油脂积累,进一步探究其作为富集油脂微藻的潜力。研究发现：在含有NH₄CL的培养基中,Chlamydomonas sp.ICE-L生长速率最大;在含有NH₄NO₃的条件下,获得了最大干重量0.28 g/L。最高油脂含量0.21 g/g是在缺氮条件下获得,同时得到干重0.24 g/L。在多不饱和脂肪酸的产出方面,NH₄NO₃和NH₄Cl为氮源培养基时要好于缺氮和KNO₃培养基,在NH₄NO₃和NH₄Cl为氮源的培养条件下ICE-L胞内C18：3和C20：5的含量高。比较而言,缺氮和KNO₃培养基时C16：0、C18：1和C18：2的含量要高。  相似文献   

Effect of high salinity on cell growth and protein production of Antarctic ice microalgae Chlamydomonas sp. ICE-L     

郑洲  缪锦来  阚光峰  金青  丁燏  刘芳明  王守强  王以斌 《极地研究(英文版)》2010,21(1):81-90

Antarctic ice microalgae Chlamydomonas sp. ICE-L can survive and thrive in Antarctic sea ice. In this study, Chlamydomonas sp. ICE-L could survive at the salinity of 132‰ NaCl. SDS-PAGE showed that the density of 2 bands (26 and 36 kD) decreased obviously at the salinity of 99‰ NaCl compared to at the salinity of 33‰ NaCl. The soluble proteins in Chlamydomonas sp. ICE-L grown under salinity of 33‰ and 99% NaCl were compared by 2-D gel electrophoresis. After shocking with high salinity, 8 protein spots were found to disappear, and the density of 28 protein spots decreased. In addition, 19 protein spots were enhanced or induced, including one new peptide (51 kD). The changes of proteins might be correlated with the resistance for Chlamydomonas sp. ICE-L to high salinity.  相似文献   

UV-B短期照射对北极冰川小球藻Chlorella sp.的生物学效应          下载免费PDF全文

彭小伟  何剑锋  罗玮  李运广  蔡明红 《极地研究》2010,22(2):164-173

以一种分离自北极冰川融水的淡水微藻（Chlorella sp.）为实验材料,设置两个不同强度UV-B（45μw/cm2、76μw/cm2）照射处理、每4小时取样检测,对其生化组分进行分析,了解微藻对UV-B辐射的响应。研究结果显示小球藻经辐射处理后,其生化成分发生变化：（1）在较低强度的UV-B（45μw/cm2）辐射下,叶绿素a（Chla）和类胡萝卜素（Car）含量先升后降,且在4h时有最大值;高强度UV-B（76μw/cm2）辐射下,Chla和Car含量随辐射时间延长基本呈下降趋势。（2）超氧阴离子（O2?ˉ）和丙二醛（MDA）含量基本随辐照时间的延长和辐照强度的增大提高,过氧化氢（H2O2）含量变化虽随辐照时间的延长呈上升趋势,但直到12h是才表现为明显的提高。（3）超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性在低UV-B处理下,随辐照时间的延长呈升高趋势;在高UV-B辐射下,SOD活性先升后降,CAT活性上下波动。(4)UV-B辐射使小球藻脂肪酸组成发生了变化。这些结果表明：UV-B,尤其是高强度或较高剂量的UV-B会对北极冰川微藻带来不利的影响。  相似文献   

Antioxidant and biotransformation responses in Liza aurata under environmental mercury exposure - relationship with mercury accumulation and implications for public health     

Guilherme S  Válega M  Pereira ME  Santos MA  Pacheco M 《Marine pollution bulletin》2008,56(5):845-859

This study was carried out in the Laranjo basin (Ria de Aveiro, Portugal), an area impacted by mercury discharges. Liza aurata oxidative stress and biotransformation responses were assessed in the liver and related to total mercury (Hg_t) concentrations. A seasonal fish survey revealed a sporadic increase in total glutathione (GSH_t) and elevated muscle Hg_t levels, although Hg levels did not exceed the EU regulatory limit. As a complement study, fish were caged for three days both close to the bottom and on the water surface at three locations, and displayed higher Hg_t levels accompanied by increased GSH_t content and catalase activity as well as EROD activity inhibition. The bottom group displayed higher hepatic Hg_t and GSH_t contents compared with the surface group. Globally, both wild and caged fish revealed that the liver accumulates higher Hg_t concentrations than muscle and, thus, better reflects environmental contamination levels. The absence of peroxidative damage in the liver can be attributed to effective detoxification and antioxidant defense.  相似文献   

Adsorption of tributyltin by tributyltin resistant marine Pseudoalteromonas sp. cells     

Haruo Mimura  Ryusei Sato  Yuichi Furuyama  Akira Taniike  Masahiro Yagi  Kazutoshi Yoshida  Akira Kitamura 《Marine pollution bulletin》2008,57(6-12):877

The isolate, Pesudoalteromonas sp. TBT1, could grow to overcome the toxicity of tributyltin chloride (TBTCl) up to 30 μM in the absence of Cl⁻ in the medium until the cells reached an exponential phase of growth. The viability, however, was reduced after the cells reached a stationary phase. The degradation products, such as dibutyltin (DBT) and monobutyltin (MBT), were not detected in the growth medium, indicating that the isolate has no ability to degrade TBT into less toxic DBT and MBT. Up to about 10^7.5 TBT molecules were adsorbed by a single cell. The observation of morphological changes with an electron microscope showed that the cell surface became wrinkled after exposure to the lethal concentration of 10 mM TBTCl. These results indicate that the resistance of the isolate toward the toxicity of TBTCl is not related to the unique cell surface, which seems to play an important role in preventing the diffusion of TBTCl into the cytoplasm.  相似文献   

太湖环棱螺对两种常见沉水植物生长的影响   总被引：2，自引：1，他引：1  

白秀玲  谷孝鸿  张钰 《湖泊科学》2007,19(1):98-102

环棱螺(Bellamya sp.)是太湖常见的一类软体动物.本研究通过室内实验,探讨环棱螺对轮叶黑藻(Hydrilla verticillata Royle)和伊乐藻(Elodea nuttalli ST John)生长的影响及水体营养盐含量的变化.结果表明,三种处理情况下,单位质量伊乐藻增加的数量分别为:H组0.475 g,L组0.106 g,C组0.021 g,单位质量轮叶黑藻增加的数量分别为:H组0.704 g,L组0.663 g,C组0.478 g.从实验前后两种沉水植物的长度和分蘖数变化来看,H组最高,L组与C组分别次之,所以不论生物量、长度还是分蘖数的变化量,与环棱螺共存的伊乐藻和轮叶黑藻的变化都高于对照组中两种沉水植物的变化.环棱螺新陈代谢促进水体中溶解态氮磷含量增加,三种情况下水生植物的初级生产力都相当,由此可推测环棱螺通过新陈代谢,一定程度上促进了两种沉水植物的生长.  相似文献   

太湖螺类的实验生态学研究——以环棱螺为例   总被引：3，自引：0，他引：3  

白秀玲  谷孝鸿  张钰 《湖泊科学》2006,18(6):649-654

通过室内实验探讨环棱螺营养盐释放的影响因子及环棱螺对水体悬浮物、透明度及浮游植物的影响等生态特性．结果表明:温度及进食状况对环棱螺的营养盐释放均有显著影响．15℃时,环棱螺氨氮的释放速率约为55．60±4．95μg／(g·d),磷酸根的释放速率约为2．073±0．120μg／(g·d);而25℃时,环棱螺磷酸根的释放速率是2 819±0．075μg／(g·d),表明在一定温度范围内,环棱螺营养盐的释放速率随温度升高而增加,且进食状态下环棱螺的营养盐释放速率高于饥饿状态．此外,环棱螺虽在短时间内可提高水体的透明度,但其释放的营养盐可引起局部水体溶解态氮磷含量的增加,在没有其它初级生产者争夺营养盐和光照等资源的情况下,水体中藻类的再生速率会加快,环棱螺难以对藻类的增殖起到抑制作用．南此建议在利用螺类进行生态调控的过程中,必须与沉水植物的恢复紧密结合,通过合理的空间搭配,实现水生态系统的恢复与健康发展．  相似文献   

圆尾斗鱼食物选择性的实验研究   总被引：1，自引：0，他引：1  

郭先武  周洁  杨灵 《湖泊科学》1995,7(2):173-177

在实验条件下,圆尾斗鱼优摄食纹幼虫和摇蚊幼虫,对食物的选择性明显,同时表明,用数量指标,以选择指数和Ｘ＾２检验能较客观地,可靠地说明食物选择性的大小。  相似文献   

利用不同浓度啤酒废水培养塔胞藻的生物学效应研究   总被引：2，自引：0，他引：2  

张建民  张倩  赵宏  王相伟 《海洋湖沼通报》2008,(4)

本文主要探讨了以不同浓度的啤酒废水培养海洋微藻(塔胞藻)后的生物学效应,其目的是为合理开发利用啤酒废水,避免环境污染及海洋微藻的产业化提供必要的理论依据。方法:以不同浓度(0、10%、20%、30%、40%、50%、60%)的啤酒废水替代海水配制培养基,培养海洋微藻,当培养液内细胞数达到一定密度后,再转到新的培养基中,即为下一轮;这样连续培养了4轮。结果发现,在前3轮培养中,当培养基中啤酒废水浓度在50%以下时,随着啤酒废水浓度升高,藻细胞的生长繁殖明显加快,细胞体积增大,细胞内多种重要成分(如蛋白质、可溶性糖、叶绿素等)含量均显著提高;当啤酒废水浓度超过50%时,上述效应有所下降。但培养至第4轮时,各种生理指标逐渐恢复到原来的状态。  相似文献   

浙江晚泥盆世的石松类(Lycopodiales)化石     

陈其奭 《浙江国土资源》1999,(2)

本文描述了萧山虎山和长兴泗安晚泥盆世地层中的石松类化石,共有6个属9个种,其中包括1个新属和3个新种,分别:SpinolepidodendronhangzhouenseGen.etsp.nov,SublepidodendronXianshanensesp.nov,Eolepi-dodendronhushanensesp.nov.。认为浙江晚泥盆世植物化应归于以Leptopnloeumrhombicum-Sublepidodendronxi-aoshanense—Xihuphyllummegalofolium为代表的组合。  相似文献