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11.
Marine proteomics: generation of sequence tags for dissolved proteins in seawater using tandem mass spectrometry 总被引：3，自引：1，他引：3
Matthew J. Powell Jennifer N. Sutton Carlos E. Del Castillo Aaron T. Timperman 《Marine Chemistry》2005,95(3-4):183-198
Dissolved proteins in seawater samples from the Gulf of Mexico were concentrated using tangential flow ultrafiltration and methanol/chloroform/water precipitation. Following concentration and purification, two different separation methods were employed. In one method, intact proteins were separated by SDS–PAGE and digested enzymatically in-gel. In the second method, the peptides resulting from a solution proteolytic digest of the whole protein pellet mixture were separated by capillary HPLC. In both methods, the final chromatographic separation was coupled on-line with a mass spectrometer using an electrospray interface, and peptide CID spectra were collected using tandem mass spectrometry (MS). De novo sequencing of the peptide tandem mass spectra generated short amino acid sequences (peptide tags) that were used to search databases for protein class and source information. Trends of conserved sequences for two specific classes of proteins were observed: membrane/envelope proteins and enzymes. Similarity searching of peptide tags produced identification of conserved sequences from several protein homologues originating from many different species, including: long chain fatty acyl CoA synthetase, anthranilate synthase, ribulose bisphosphate carboxylase, and luminal binding protein. These results provide new insight into the sources and production mechanisms for dissolved organic matter (DOM), as there is direct evidence for dissolved proteins other than the bacterial outer membrane proteins reported by Tanoue et al. Furthermore, the data presented herein support the idea that physical protection and selective preservation are not mutually exclusive survival mechanisms, but rather these two models are dependent upon one another for explaining the survival of refractory dissolved proteins in seawater. 相似文献
12.
Dissolved (<1 kDa) and colloidal (1 kDa-0.45 μm) size fractions of sulfate, organic carbon (OC), phosphate and 17 metals/metalloids were investigated in the acidic Vörå River and its estuary in Western Finland. In addition, geochemical modelling was used to predict the formation of free ions and complexes in these waters. The sampling was carried out during high-water flow in autumn and in spring when the abundantly occurring acid sulfate (AS) soils in the catchment area are extensively flushed. Based on the high concentrations of sulfate, acidity and several metals, it is clear that the Vörå River and its estuary is strongly affected by AS soils. The high dissolved form of metals limits also the existence of fish and other organisms in this estuary, and certainly also in other similar shallow brackish estuaries elsewhere in the Gulf of Bothnia. However, generally already <20% saline sea water reduces the concentration for OC and several elements (Al, Cu, Cr, Fe, Pb, PO4 and U) by half and c. 20–30% saline sea water is needed to halve concentrations of Cd, Co, Mn, Ni and Zn. Consequently, these elements as well as organic matters were rapidly precipitated in the estuary, even after mixing with fairly small amounts of the alkaline brackish sea water. Aluminium, Cu, Fe and U most likely precipitate together with organic matter closest to the river mouth. Manganese is relatively persistent in solution and, thus, precipitates further down the estuary as Mn oxides, which concomitantly capture Ba, Cd, Co, Cu, Ni and Zn. In the inner estuary, the high contents of Al is as important than Fe in removing PO4 and, thus, also reducing the risk of algae blooms in near coastal areas influenced by AS soils in the Gulf of Bothnia. Moreover, the dispersion of metals far out in the estuary is dependent on hydrological conditions, i.e. with high flows the plume of metal-rich water will spread further out in the estuary. Furthermore, the extensive drainage of the catchment and subsequent artificial enlargement of the river channel during recent decades has not only enabled oxidation of sulfidic sediments, but strongly increased flow peaks that reach further out in the estuary. 相似文献
13.
The Chemical Speciation of Fe(III) in Freshwaters 总被引：1，自引：0，他引：1
Dialysis and chemical speciation modelling have been used to calculate activities of Fe3+ for a range of UK surface waters of varying chemistry (pH 4.3–8.0; dissolved organic carbon 1.7–40.3 mg l−1) at 283 K. The resulting activities were regressed against pH to give the empirical model: . Predicted Fe3+ activities are consistent with a solid–solution equilibrium with hydrous ferric oxide, consistent with some previous studies
on Fe(III) solubility in the laboratory. However, as has also sometimes been observed in the laboratory, the slope of the
solubility equation is lower than the theoretical value of 3. The empirical model was used to predict concentrations of Fe
in dialysates and ultrafiltrates of globally distributed surface and soil/groundwaters. The predictions were improved greatly
by the incorporation of a temperature correction for , consistent with the temperature dependence of previously reported hydrous ferric oxide solubility. The empirical model,
incorporating temperature effects, may be used to make generic predictions of the ratio of free and complexed Fe(III) to dissolved
organic matter in freshwaters. Comparison of such ratios with observed Fe:dissolved organic matter ratios allows an assessment
to be made of the amounts of Fe present as Fe(II) or colloidal Fe(III), where no separate measurements have been made.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
14.
Tamer Coskun Asuman Yildirim Cigdem Balcik Neslihan Manav Demir Eyup Debik 《洁净——土壤、空气、水》2013,41(5):463-468
In the following study, two methods were employed in which olive mill wastewaters were treated by using reverse osmosis membranes (BW30 and XLE). In the first, wastewater was centrifuged and then passed through the reverse osmosis whereas in the second, an ultrafiltration was placed between the centrifuge and the reverse osmosis. The reverse osmosis experiments were conducted under 10, 15, 20, and 25 bar. The chemical oxygen demand (COD) removal efficiencies under 25 bar were found to be 97.5% for both BW30 and XLE membranes. The highest conductivity removal rates obtained under 25 bar for BW30 and XLE membranes were found to be 95.6 and 96.2%, respectively. As for the highest permeation flux values obtained under 25 bar, they were separately determined for BW30 and XLE as 15.3 and 21.2 L m?2 h?1, respectively. The performances of the membranes were also evaluated in terms of their mass transfer coefficients. According to this, all mass transfer coefficients were found to be <1 and also in proximate to one another; this clearly reflects the results as COD and conductivity removals were approximate and there was a lack of any significant difference, whether ultrafiltration was applied or not. 相似文献
15.
An ultrafiltration methodology and the results of its application on Elbe river water are presented. Compared with other methods, Ultrafiltration allows fractionation of the molecular-weight fractions of DOC for higher volumes of solution in a shorter time. To characterize the molecular-weight fractions, the sum parameter DOC (β(DOC)) and the absorption coefficient at 254 nm a254 were measured, together with determination of a254/ β(DOC) for further characterization of the DOC fractions and to provide information about changes in parts of the UV-active substances of the total dissolved organic material. In the period May 1992 to November 1994, the DOC molecular-weight composition of 65 samples of Elbe river water were determined. At two sampling locations (Torgau-East and Meissen), the DOC content β(DOC) was approximately 6 mg/L. The high-molecular weight fraction (> 10000 g/mol) gave a mean of 13% of the total DOC content β0(DOC). The measurements showed that the molecular composition of DOC in the Elbe does not greatly differ. There was also no clear seasonal influence on the molecular-weight composition of the DOC content. During the period of investigation, the percentage part of β(DOC) and a254/β(DOC) of the high-molecular weight fraction of the total dissolved organic material increased, while the a254/β(DOC) values of the middle molecular-weight fraction remained fairly constant. The dependence of β(DOC) on discharge of the Elbe river is low. Changes in molecularweight composition of DOC were only observed during the steepest increase in river discharge during a flood event, with another spectrum of substances responsible for the increase in β(DOC) when compared with the β(DOC) for low or middle water level. 相似文献
16.
This paper reports changes in dissolved organic carbon concentration β(DOC) and the relation between UV-active and non-UV-active components determined for Elbe river water and river bank infiltrate in the Torgau river basin between 1992 and 1994. Using an ultrafiltration method, the fractionation of the DOC content was obtained for the fractions > 10 000 g/mol, 1 000…10 000 g/mol, and < 1 000 g/mol. The spectral absorption coefficient at 254 nm a254 of the molecular-weight fractions was also measured. The mean total DOC concentration of Elbe river water decreased from 6.0 mg/L to below 3.9 mg/L along two investigated flowpaths. Two thirds of the decrease occurred within the first few metres of the river bed and one third along the 350 m length of the groundwater flowpaths. The a254 values showed a significant decrease from 14.8 1/m in Elbe river water to 7.8 1/m in the aquifer. Along a flowpath, the proportion of low-molecular weight fraction of DOC increased, the proportion of high-molecular weight fraction decreased, and the proportion of the 1 000…10 000 g/mol molecular-weight fraction remained relatively stable. The Elbe river water contained the main portion of UV-active compounds in the fraction 1 000…10 000 g/mol, and this was also the case for samples of river infiltrate. For the high-molecular weight fraction, mainly non-UV-active compounds were attenuated in the river bed sediment. 相似文献
17.
Colloidal materials and macromolecules that are less than 0.45 mircons in diameter can act to transport both hydrophobic and hydrophilic contaminations in subsurface waters. An automated hollow-fibre ultrafiltration apparatus is described and evaluated regarding its ability to physically size and isolate colloidal materials from natural waters. Sufficient size-fractionated colloidal samples can be obtained from 60 litre samples to perform inorganic, organic, and radionuclide characterizations, as well as allow the binding capacities of humic and fulvic materials to be evaluated. Samples processed in the field with the automated system were compared with laboratory analyses using single cartridge hollow-fibre ultrafilters and found to agree well with each other. Typical sample reproducibilities were with 15 per cent. The results indicate that field collection of colloidal materials is feasible with minimal sample perturbation using the automated sampler. 相似文献
18.
Seasonal variations in dissolved organic carbon concentrations and characteristics in a shallow coastal bay 总被引：8，自引：0，他引：8
Dissolved organic matter (DOM) composition and dynamics in temperate shallow coastal bays are not well described although these bays may be important as local sources of organic carbon to ocean waters and are often sites of economically-important fisheries and aquaculture. In this study surface water samples were collected on a monthly to bi-monthly basis over two years from a mid-Atlantic coastal bay (Chincoteague Bay, Virginia and Maryland, USA). Dissolved organic carbon (DOC) concentrations and light absorbance characteristics were measured on sterile-filtered water, and high-molecular weight (> 1 kDa) dissolved OM (DOM) was isolated to determine stable isotope composition and molecular-level characteristics. Our time series encompassed both a drought year (2002) and a year of above-average rainfall (2003). During the dry year, one of our sites developed a very intense bloom of the brown tide organism Aureococcus anophagefferens while during the wet year there were brown tide bloom events at both of our sampling sites. During early spring of the wet year, there were higher concentrations of > 1 kDa DOC; this fraction represented a larger proportion of overall DOC and appeared considerably more allochthonous. Based upon colored dissolved organic matter (CDOM) and high-molecular weight DOM analyses, the development of extensive phytoplankton blooms during our sampling period significantly altered the quality of the DOM. Throughout both years Chincoteague Bay had high DOC concentrations relative to values reported for the coastal ocean. This observation, in conjunction with the observed effects of phytoplankton blooms on DOM composition, indicates that Chincoteague Bay may be a significant local source of “recently-fixed” organic carbon to shelf waters. Estimating inputs of DOC from Chincoteague Bay to the Mid-Atlantic Bight suggests that shallow productive bays should be considered in studies of organic carbon on continental shelves. 相似文献