基于全基因组重测序技术的浙江近岸海域耐盐金黄色葡萄球菌(Staphylococcus aureus)耐药机制解析<     

张旨轩  王子言  王泽  刘岩  刘松怡  钱鹏宇  叶欢  韩姣姣  周君  苏秀榕 《海洋与湖沼》2022,53(2):394-404

金黄色葡萄球菌(Staphylococcus aureus)是近岸海域海水中的主要病原菌,严重威胁接触者的安全。抗生素处理是治疗金黄色葡萄球菌感染的重要手段,其耐药性的发生受到了高度重视。采用全基因组重测序与KEGG富集分析结合的方法,对红霉素(erythromycin)、氯霉素(chloramphenicol)和万古霉素(vancomycin)处理后的耐盐金黄色葡萄球菌(S.aureus ZS01)和不耐盐金黄色葡萄球菌(S.aureus 502A)进行耐药机制研究。结果表明,S.aureus 502A经抗生素处理后发生突变的程度大于S.aureus ZS01,二者在经过氯霉素处理发生了更大程度的突变。红霉素、氯霉素和万古霉素处理主要影响了金黄色葡萄球菌的致病能力;红霉素和氯霉素可能通过影响金黄色葡萄球菌脂类的代谢引起其耐药性的变化。除此之外,三种抗生素处理均出现了较多TIGR01741家族蛋白和假设蛋白基因的突变,推测与菌株的耐药性和致病性相关。耐盐金黄色葡萄球菌可通过外排系统作用产生红霉素耐药性,不耐盐菌株因细胞壁成分相关基因的突变提高了对万古霉素的耐受性。研究结果可为耐盐和不耐盐金黄色葡萄球菌的耐药机制及抗生素对金黄色葡萄球菌致病性影响的研究提供基础数据。  相似文献   

高黎贡山羚牛生境适宜性评价及廊道建设     

冯鸿能  杨德宏  饶定齐  魏旋 《测绘通报》2023,(1):134-140

以高黎贡山羚牛为研究对象,选取了植被类型、植被覆盖度、海拔、坡度、水源、土地覆被、主要道路、居民点8个影响因子,基于GIS技术,构建阻力面,从而探测多因子影响作用下高黎贡山羚牛适宜生境。结果表明：区域内羚牛的适宜生境面积整体占比较少,适宜区域主要分布在研究区的中西部及西南地区,在北部区域也有零散分布。羚牛的迁徙受到人类活动的干扰及道路阻隔的影响,其生境遭到一定程度的破坏。结合现有羚牛栖息地分布及最小阻力模型,建立区域内羚牛的生态廊道,结果显示：廊道主要分布于高黎贡山保护区南段、马边瓦底中段、子巴、俄恰沙迪、马士打亚窟、其期北段、四克洛娃俎及木当一带。  相似文献   

Variations in spring leaf phenology and leaf freezing damage of common woody species in China北大核心CSCD     

陶泽兴  戴君虎 《地理科学进展》2023,(4):766-781

Freezing damage results in the dehydration of plant cells and reduces the photosynthetic capacity of plants, which causes significant losses to ecology and economy. Over the past 40 years, global warming has reduced the frequency and intensity of frost events while bringing forward the spring phenology of plants, increasing the exposure of their leaves and flowers to harsh cold temperatures. Therefore, the dual effects of climate warming should be considered in order to accurately assess the changes of plant freezing damage. To date, there is no systematic analysis of plant freezing damage in different climatic regions of China. Based on phenological observation records from the China Phenological Observation Network, leaf frost damage of four common woody plants (Ulmus pumila, Robinia pseudoacacia, Salix babylonica, Fraxinus chinensis) in the spring over the past 40 years was calculated, and the spatio-temporal patterns were analyzed. We also investigated the change in the occurrence time of maximum frost damage (TMFD) and its relationship with plant phenology. The results show that: 1) Most species presented an overall trend towards an earlier leaf unfolding date, and the advancing trend was significant and greater than 1 d/a in about 60% of the regions (P<0.05). 2) The TMFD occurred earlier in 72.22%-83.03% of the regions, which was closely related to plants' earlier leaf unfolding date. The TMFD of all species advanced the most (8.3 days) in the temperate climate zone, followed by the warm temperate, subtropical, plateau, and cold temperate zones. 3) The leaves of U. pumila, R. pseudoacacia and S. babylonica suffered more freezing damage in the spring, and the most significant freezing damage was mainly found in the north of 50°N region and part of the west of the Qinghai-Tibet Plateau. In comparison, the leaves of F. chinensis suffered less frost damage due to later leaf unfolding date and stronger leaf frost resistance. With regard to interannual variations, the average freezing damage of U. pumila, R. pseudoacacia and S. babylonica increased significantly (P<0.05), but that of F. chinensis did not change obviously. In addition, the freezing damage of U. pumila and S. babylonica increased the most in the cold temperate zone, while that of R. pseudoacacia increased in about 10% of the regions in the plateau climate zone, and 3%-6% of the regions in the cold temperate, temperate, and warm temperate climate zones. The freezing damage of F. chinensis merely increased in the warm temperate zone. The results of this study can provide a reference for assessing the risk of plant freezing damage accurately and help develop regional-specific response and adaptation strategies to climate change. © 2023, Editorial office of PROGRESS IN GEOGRAPHY. All rights reserved.  相似文献   

宁南弧形构造带甘肃大沟—内蒙古乱井中上地壳电性结构          下载免费PDF全文

闵刚  程建华尹秉喜蔡学林夏时斌 《中国地质》2014,41(5):1464-1475

利用大地电磁探测方法在宁南弧形构造带内蒙古乱井—甘肃大沟段进行了中上地壳电性结构特征研究,通过二维反演获得了研究区的电性结构模型,模型清楚揭示出了该区典型的电性结构特征:(1)中上地壳电性结构整体表现为"碎块状"的构造特征,其中走廊构造带总体表现为相对低阻,北祁连碰撞造山带总体表现为相对高阻;(2)海原地震震源区的震源断裂所反映的低阻带构造样式既不像走滑断裂那样陡峭,也不像逆冲断裂那样平缓,而是兼具走滑与逆冲断裂带的双重特征;(3)研究区壳内低阻层呈现不连续分布,总体表现为南深北浅的构造样式,一般都终止于高阻地块边缘或与逆冲推覆构造的滑脱面相交汇处;(4)清水河裂褶带内发育的高阻地块可能为晚古生代或早中生代发育在元古宙基底上的古隆起,为该区燕山期构造运动提供了深部大地电磁资料证据。  相似文献   

考虑桩-土体系渐进破坏的单桩承载特性研究   总被引：1，自引：0，他引：1  

林春金  张乾青  梁发云 《岩土力学》2014,35(4):1131-1140

针对目前桩基承载特性确定时未考虑桩-土体系渐进变形过程的现状,采用现场试验和理论分析等手段对考虑桩-土体系渐进破坏的单桩承载特性展开研究。首先,利用现场试验数据对侧阻和端阻的软化特性展开深入探讨,提出了侧阻和端阻软化模型,明确了模型中各参数取值方法,并验证了其合理性。同时,根据提出的侧阻和端阻软化模型,建立了一种可考虑桩-土体系渐进破坏的单桩承载特性的迭代算法。算例分析表明,计算获得的破坏性单桩承载特性与实测值有较好的一致性,且可反映侧阻和端阻的破坏特性。实际工程中,结合单桩承载特性分析算法,可根据单桩的实际受力特点灵活选用不同形式的侧阻和端阻荷载传递函数来分析不同桩顶荷载水平下的单桩受力特性。  相似文献   

豫陕鄂交界区县域综合发展水平测度及障碍因子分析   总被引：1，自引：0，他引：1  

李许红  王永生  刘释疑 《地理科学》2022,42(10):1757-1766

以豫陕鄂交界区为研究对象,测度2000—2017年豫陕鄂交界区县域的综合发展水平,探析其空间分异特征、演化趋势和障碍因素。结果表明：① 2000—2017年,豫陕鄂交界区县域综合发展水平逐步提升,年均增速为6.08%,县域间发展的空间差异逐渐增大;② 县域综合发展水平在空间上呈正相关性,热点显著区呈现由东南部向西南部再向东南部迁移变化特征,冷点显著区在2005年前主要分布在安康市和商洛市,之后主要聚集在南阳市;③ 人文和经济要素为中度和中高障碍水平,资源和环境要素为极低和低障碍水平;随着时间的推移,障碍因子在不同县域空间阻力的分异特征更加显著。④ 豫陕鄂交界区县域发展空间阻力模式可划分为3种类型,其中人文资源要素制约型是主要的空间阻力模式。最后,基于不同空间阻力模式特征解析,提出了分区发展策略。  相似文献   

“水-能源-粮食”视角下粤港澳大湾区生态安全格局的构建及优化     

毛源远  张正栋  董剑彬  杨阳  曹君  陈宋佳  匡腾飞 《热带地理》2022,42(2):328-338

从“水－能源－粮食”3个维度对粤港澳大湾区的生态系统服务进行评价,利用珞珈一号夜光数据修正生态阻力面,运用最小累积阻力模型构建并优化湾区的生态安全格局。结果表明：1）粤港澳大湾区生态源地总面积为9 626.1 km²,主要分布在研究区东部和西部的山地、丘陵地区,对应位于江门市、惠州市中部和肇庆市;2）生态廊道共计38条,总长度为2 023.09 km,其中最短为11.76 km,最长为304.99 km,集中于植被覆盖较好的山地丘陵地区,中部无廊道分布;3）生态源地辐射区面积为28 929.5 km²,达到湾区总面积的51.88%,若要实现60%的覆盖率,则需要至少新增生态源地辐射面积4 524.856 km²;4）为了更好地优化粤港澳大湾区生态安全格局,提出在惠州市北部和南部新增两处生态源地辐射区,并构建以“一带、一轴、四组团”为核心的生态安全格局优化模式。  相似文献   

The canopy conductance of a boreal aspen forest,Prince Albert National Park,Canada     

P. D. Blanken  T. A. Black 《水文研究》2004,18(9):1561-1578

Annual fluxes of canopy‐level heat, water vapour and carbon dioxide were measured using eddy covariance both above the aspen overstory (Populus tremuloides Michx.) and hazelnut understory (Corylus cornuta Marsh.) of a boreal aspen forest (53·629 °N 106·200 °W). Partitioning of the fluxes between overstory and understory components allowed the calculation of canopy conductance to water vapour for both species. On a seasonal basis, the canopy conductance of the aspen accounted for 70% of the surface conductance, with the latter a strong function of the forest's leaf area index. On a half‐hour basis, the canopy conductance of both species decreased non‐linearly as the leaf‐surface saturation deficits increased, and was best parameterized and showed similar sensitivities to a modified form of the Ball–Berry–Woodrow index, where relative humidity was replaced with the reciprocal of the saturation deficit. The negative feedback between the forest evaporation and the saturation deficit in the convective boundary layer varied from weak when the forest was at full leaf to strong when the forest was developing or loosing leaves. The coupling between the air at the leaf surface and the convective boundary layer also varied seasonally, with coupling decreasing with increasing leaf area. Compared with coniferous boreal forests, the seasonal changes in leaf area had a unique impact on vegetation–atmosphere interactions. Copyright © 2004 John Wiley & Sons, Ltd.  相似文献   

蒸发的阻抗模型讨论   总被引：1，自引：0，他引：1  

陈发祖 《地理研究》1991,10(2):1-10

蒸发的阻抗模型,从简单的单层模型到复杂的多层模型,应用日益广泛。目前文献中流行一种看法:单层阻抗模型没有牢固的物理基础,但简单易行;而多层模型才具备可靠的物理基础,不过缺乏实际应用价值。本文对此作了讨论,认为两种模型均有较好的物理基础,但各有自己最适合的应用范围,且两者又都具有共同的难点。  相似文献   

Measuring the flow resistance of submerged grass     

C. A. M. E. Wilson  M. S. Horritt 《水文研究》2002,16(13):2589-2598

Through laboratory experiments conducted in a grass‐lined flume, the hydraulic resistance of grass is measured and quantified. For the grass examined, it is found that Manning's n value is greater than those recommended in well‐established texts such as Chow (1959. Open Channel Flow. McGraw‐Hill: Singapore), relatively lower than those predicted by n‐UR methods, but corresponds well with the value found from calibration studies of two‐ and three‐dimensional numerical models. The assumption of a uniform Manning's n value with flow depth, which is often made in numerical modelling, may be invalid depending on the relative submergence of the vegetation. Drag coefficients are evaluated for a method applicable to three‐dimensional numerical models. Further detailed experimental investigation and application of these approaches within a numerical modelling framework is now recommended. Copyright © 2002 John Wiley & Sons, Ltd.  相似文献