The Hydrolyzation of Collagen by Fucoidan Oligosaccharide''s Complex with CeⅣ     

XU Jiachao  GAO Xin  ZHANG Zhaohui  LI Zhaojie  HUO Lihua  XUE Changhu 《中国海洋大学学报(英文版)》2006,5(2)

Fucoidan is such a polysaccharide that its hydroxies are easy to combine with lanthanons ion (CeⅣ) to form complex. This work obtained the complexes of three fucoidan oligosaccharides with different molecular weights F1( ＞5 000), F2(1 000-5 000) and F3( ＜ 1 000) by hydrolyzing Oligosaccharide collagen with sulfuric acid. It is found that the fucoidan oligosaccharide F3 can form complex with more CeⅣ than F1 and F2. Hydrolyzing collagen with the complex was carried out to produce amino acid and peptides. All the three fucoidan oligosaccharide complexes with CeⅣ( F1, F2, F3) can catalyze by the artificial hydrolytic enzyme, and the activity of the complex of F3 is the highest.  相似文献   

海藻胶低聚寡糖的化学氧化制备纯化技术及保水理化性质分析     

李海波  谢超  丁晨  黄菊  裘晓华  林琳 《海洋与湖沼》2015,46(4):915-921

海藻胶低聚寡糖是从马尾藻等藻类中提取出来的一种多糖类物质,具有抗氧化及保水等生物活性。本文采用氧化降解技术对海藻胶低聚寡糖的制备工艺进行了研究,通过对氧化过程中氧化剂浓度、反应温度、反应时间等进行研究,结果表明:当氧化剂H2O2添加浓度控制在6%左右,温度控制在60°C,时间控制在8h时,海藻胶降解为低聚寡糖的产率最高,达到60%左右。进一步对海藻胶低聚寡糖进行纯化,结果表明当温度控制在27°C,时间在2h时,分子量为6000—8000Da海藻胶低聚寡糖分离效率最高,达到70%以上,并对6000—8000Da海藻胶低聚寡糖的保水理化性能进行分析,结果表明6000—8000Da海藻胶低聚寡糖具有良好的保水性能。本研究可为开发一种安全、高效、节能、环保,适用于鱿鱼和虾仁等的生物保水剂提供理论基础。  相似文献   

制备条件对聚乙烯醇-壳聚糖凝胶性能的影响   总被引：1，自引：0，他引：1  

温燕梅  李思东  钟杰平  章超桦  梁泽萍 《广东海洋大学学报》2007,27(4):74-77

以戊二醛为交联剂,由聚乙烯醇(PVA)和壳聚糖(CTS)混合交联得到一种水凝胶,考察了聚乙烯醇和壳聚糖的配比、交联剂用量和反应时间对聚乙烯醇/壳聚糖凝胶吸水率的影响。通过单因素实验,得出了聚乙烯醇/壳聚糖凝胶具有最佳溶胀性能时的条件:聚乙烯醇与壳聚糖的摩尔比为6∶1,交联剂戊二醛的体积分数为0.05%,反应时间9h。测定了该凝胶在不同pH值下的吸水率和吸附铜离子的性能,得出聚乙烯醇/壳聚糖凝胶在酸性介质的吸水率远远大于碱性介质,对铜离子(Cu2+)的吸附量比壳聚糖凝胶提高了33.7%,因此该凝胶吸附剂在工业废水处理重金属离子方面具有一定的优越性。  相似文献   

Identification of AOSC-binding proteins in neurons     

刘明  聂琴  辛现良  耿美玉 《中国海洋湖沼学报》2008,26(4):394-399

Acidic oligosaccharide sugar chain （AOSC）, a D-mannuronic acid oligosaccharide, derived from brownalgaepolysaccharide, has been completed Phase I clinical trial in China as an anti-Alzheimer＇s Disease （AD） drug candidate. The identification of AOSC-binding protein（s） in neurons is very important for understanding its action mechanism. To determine the binding protein（s） of AOSC in neurons mediating its anti-AD activities, confocal microscopy, affinity chromatography, and liquid chromatography-tandem mass spectrometry （LC-MS/MS） analysis were used. Confocal microscopy analysis shows that AOSC binds to SH-SY5Y cells in concentration-, time-, and temperature-dependent fashions. The AOSC binding proteins were purified by affinity chromatography and identified by LC-MS/MS analysis. The results showed that there are 349 proteins binding AOSC, including clathrin, adaptor protein-2 （AP-2） and amyloid precursor protein （APP）. These results suggest that the binding/entrance of AOSC to neurons is probably responsible for anti-AD activities.  相似文献   

Preparation and in vitro Release Performance of Sustained-release Captopril/Chitosan-gelatin Net-polymer Microspheres     

ZHOU Li XU Junming SONG Yimin GAO Yuanyuan CHEN Xiguang 《中国海洋大学学报(英文版)》2007,6(3):249-254

The captopril/Chitosan-gelatin net-polymer microspheres(CTP/CGNPMs) were prepared using Chitosan(CTS) and gelatin(GT) by the methods of emulsification,cross-linked reagent alone or in combination and microcrystalline cellulose(MCC) added in the process of preparation of microspheres,which aimed to eliminate dose dumping and burst phenomenon of microspheres for the improvement of the therapeutic efficiency and the decrease of the side effects of captopril(CTP). The results indicated that CTP/CGNPMs had a spherical shape,smooth surface and integral structure inside but no adhesive phenomena in the preparation. The size distribution ranged from 220 μm to 280 μm. The CTP release test in vitro demonstrated that CTP/CGNPMs played the role of retarding the release of CTP compared with ordinary CTP tablets. The release behaviors of CGNPMS were influenced by preparation conditions such as experimental material ratio(EMR) and composition of cross linking reagents. Among these factors,the EMR(1/4),CLR(FA SPP) and 0.75% microcrystalline cellulose(MCC) added to the microspheres constituted the optimal scheme for the preparation of CTP/CGNPMs. The ER,DL and SR of CTP/CGNPMs prepared according to the optimal scheme were 46.23±4.51%,9.95±0.77% and 261±42%,respectively. The CTP/CGNPMs had the good characteristics of sustained release of drug and the process of emulsification and cross-linking were simple and stable. The CGNPMs are likely to be an ideal sustained release formulation for water-soluble drugs.  相似文献   

昆布寡糖对2型糖尿病大鼠的实验作用   总被引：1，自引：0，他引：1  

侯庆华  宋文东  王浩  纪丽丽 《广东海洋大学学报》2009,29(4):46-50

从沿海藻类昆布(Thallus laminariae)中提取昆布多糖,经降解后得昆布寡糖。采用高脂饲料加小剂量链脲佐菌素法诱导SD大鼠2型糖尿病动物模型,成模后大鼠分为正常对照组,阴性对照组,二甲双胍组,昆布寡糖高、中、低剂量组,灌胃给予寡糖溶液8周,定期观察体重并采集血液标本进行检测。结果表明,昆布寡糖对糖尿病大鼠有明显的降低血糖,升高胰岛素,增加体重,降低甘油三酯、总胆固醇、游离脂肪酸,升高高密度脂蛋白胆固醇的作用,但对低密度脂蛋白胆固醇的影响不明显。可见,昆布寡糖对2型糖尿病大鼠具有明显治疗作用。  相似文献   

褐藻寡糖激发子诱导烟草抗低温作用研究   总被引：3，自引：0，他引：3  

刘瑞志  江晓路  管华诗 《中国海洋大学学报(自然科学版)》2009,39(2)

研究褐藻寡糖在低温下对烟草的保护作用,探讨褐藻寡糖作用机理,将褐藻寡糖溶液均匀喷施到烟草叶面后置于(4±1)℃下进行低温胁迫,间隔不同时间检测相关指标.研究发现,浓度是影响褐藻寡糖抗冻性能的重要因素.0.05%,0.20%和0.30%褐藻寡糖具有诱导激发因子作用,能够诱导烟草CAT、SOD和POD活力提高,清除体内产生的氧自由基,保护细胞膜和叶绿素结构,减少烟草叶片损伤,提高烟草耐低温能力,其中以0.20%褐藻寡糖诱导效果最好;0.10%褐藻寡糖具有抗冻保护剂作用,在24 h内烟草各项指标均没有明显变化,可保护细胞免受低温伤害,但48 h时冻伤指标增强,其保护作用减弱或者消失;高浓度1.00%褐藻寡糖具有毒副作用,在烟草细胞表面形成较强渗透势,胞内物质外渗严重,烟草细胞结构受到破坏,加速低温下烟草叶片损伤.  相似文献   

羧甲基壳聚糖水凝胶制备及在药物控释中的应用   总被引：8，自引：0，他引：8  

徐甲坤  毕彩丰  范玉华  郭锋  何雪涛  刘冬雪 《中国海洋大学学报(自然科学版)》2007,37(3):503-506

研究羧甲基壳聚糖水凝胶(CMCS-GA)对阿司匹林的吸附性能及其在药物控释系统中的应用。以羧甲基壳聚糖作为骨架材料,采用戊二醛交联法制备CMCS-GA,进而制备阿司匹林载药凝胶。测定凝胶在人工肠液中的溶胀动力学特点,观察载药凝胶在人工肠液中的体外释放行为。在pH=7.4的人工肠液中CMCS-GA 2 h达最大溶胀度1.38。CMCS-GA的载药量为4.6 mg/g,凝胶在人工肠液中96 h的累积释放度为99.91%,具有优良的缓释性能,药物的体外释放模型符合一级动力学方程。  相似文献   

酶法制备几丁寡糖和壳糖研究现状与进展   总被引：15，自引：0，他引：15  

王扬  娄永江  杨文鸽 《海洋学研究》2001,19(4):40-45

对用酶法制备几丁寡糖和壳寡糖研究现状与进展进行了综述。几丁质酶、壳聚糖酶、溶菌酶和 N-乙酰葡萄糖胺酶等专一性的水解酶及糖酶、蛋白酶、脂肪酶等水解酶对几丁质和壳聚糖都具有部分或完全非专一性水解作用。介绍了这些酶类的作用机理及相关微生物的种类和作用条件  相似文献   

The mineralization of chitin in the sediments of the Ythan estuary, aberdeenshire, Scotland     

K. Hillman  G. W. Gooday  J. I. Prosser 《Estuarine, Coastal and Shelf Science》1989,29(6)

Reliable methods have been developed for the quantitative estimation of chitin, chitosan and chitin deacetylase in sediment samples, as well as two methods for the estimation of chitinase in these samples. The differing substrates used in the latter two methods have produced distinct ‘depth profiles’ for chitinase activity which suggest the presence of more than one chitinolytic system in the sediments of the Ythan estuary. Studies on the mineralization of chitin in these sediments have revealed a complex system with a number of unexpected features. The rate of chitin degradation was reduced in late summer, possibly due to the heavy seaweed growth which covers the mud flats at this time, although the potential degradative activities of the enzymes chitinase and chitin deacetylase were higher in summer than in winter. The proportional rates of degradation of chitin, chitosan and cellulose were similar in both the absence and presence of seaweed overgrowth, although the apparent rates of degradation of these substrates were higher in the absence of seaweeds. Core samples have indicated that the major input of chitin to the sediment ecosystem occurs via deposition at the surface, while the input of chitosan (the deacetylated polymer) occurs via its production within the sediment, primarily within the upper 5 cm of sediment. The concentrations of both these polymers decreased sharply below an ‘interface’ between aerobic and anoxic sediments.  相似文献