Evaluation of the performance of HPLC–CHEMTAX analysis for determining phytoplankton biomass and composition in a turbid estuary (Schelde, Belgium)     

Marie Lionard  Koenraad Muylaert  Michle Tackx  Wim Vyverman 《Estuarine, Coastal and Shelf Science》2008,76(4):809-817

In the upper Schelde estuary in 2002, phytoplankton biomass and community composition were studied using microscopic and pigment analyses. Chlorophyll a concentration was a good predictor of phytoplankton biomass estimated from cell counts and biovolume measurements. The phytoplankton carbon to chlorophyll a ratio, however, was often unrealistically low (<10). CHEMTAX was used to estimate the contribution of the major algal groups to total chlorophyll a. The dominant algal groups were diatoms and chlorophytes. While diatom equivalents in chlorophyll a predicted diatom biomass relatively well, chlorophyte equivalents in chlorophyll a were only weakly related to chlorophyte biomass. The pigment-based approach to study phytoplankton overestimated phytoplankton biomass in general and chlorophyte biomass in particular in late autumn and winter, when phytoplankton biomass was low. A possible explanation for this overestimation may be the presence of large amounts of vascular plant detritus in the upper Schelde estuary. Residual chlorophyll a, chlorophyll b and lutein in this detritus may result in an overestimation of total phytoplankton and chlorophyte biomass when the contribution of phytoplankton to total particulate organic matter is low.  相似文献   

Inter-annual variability in phytoplankton summer blooms in the freshwater tidal reaches of the Schelde estuary (Belgium)   总被引：1，自引：1，他引：0  

Marie Lionard  Koenraad Muylaert  Abdel Hanoutti  Tom Maris  Michle Tackx  Wim Vyverman 《Estuarine, Coastal and Shelf Science》2008,79(4):694-700

The inter-annual variability in phytoplankton summer blooms in the upper reaches of the Schelde estuary was investigated between 1996 and 2005 by monthly sampling at 10 stations. The large inter-annual variations of the chlorophyll a concentration in the freshwater tidal reaches were independent from variations in chlorophyll a in the tributary river Schelde. Summer mean chlorophyll a concentrations were significantly negatively correlated with flushing rate (Spearman correlation: r = −0.67, p = 0.05, n = 9) but not with temperature, irradiance and suspended particulate matter or dissolved silica (DSi) concentrations. During dry summers, low flushing rates permitted the development of dense phytoplankton populations in the upper part of the estuary, while during wet summers high flushing rates prevented the development of dense phytoplankton blooms. Flushing rate was also found to be important for the phytoplankton community composition. At low flushing rates, the community was dominated by diatoms that developed within the upper estuary. At high flushing rates, chlorophytes imported from the tributary river Schelde became more important in the phytoplankton community. The position of the chlorophyll a maximum shifted from the head of the estuary when flushing rates were low, to more downstream when flushing rates were high. Although DSi concentrations tended to be lower during years of high phytoplankton (mainly diatom) biomass, the relation with flushing rate was not significant.  相似文献   

Characteristics of bottom dissolved oxygen in Long Island Sound,New York     

Younjoo J. Lee  Kamazima M.M. Lwiza 《Estuarine, Coastal and Shelf Science》2008

The variability of bottom dissolved oxygen (DO) in Long Island Sound, New York, is examined using water quality monitoring data collected by the Connecticut Department of Environmental Protection from 1995 to 2004. Self-organizing map analysis indicates that hypoxia always occurs in the Narrows during summer and less frequently in the Western and the Central Basins. The primary factor controlling the bottom DO, changes spatially and temporally. For non-summer seasons, the levels of bottom DO are strongly associated with water temperature, which means DO availability is primarily driven by solubility. During summer, stratification intensifies under weak wind conditions and bottom DO starts to decrease and deviate from the saturation level except for stations in the Eastern Basin. For the westernmost and shallow (<15 m) stations, bottom DO is correlated with the density stratification (represented by difference between surface and bottom density). In contrast, at deep stations (>20 m), the relationship between oxygen depletion and stratification is not significant. For stations located west of the Central Basin, bottom DO continues to decrease during summer until it reaches its minimum when bottom temperature is around 19–20 °C. In most cases the recovery to saturation levels at the beginning of fall is fast, but not necessarily associated with increased wind mixing. Therefore, we propose that the DO recovery may be a manifestation of either the reduced microbial activity combined with the depletion of organic matter or horizontal exchange. Hypoxic volume is weakly correlated to the summer wind speed, spring total nitrogen, spring chlorophyll a, and maximum river discharge. When all variables are combined in a multiple regression, the coefficient of determination (r²) is 0.92. Surprisingly, the weakest variable is the total nitrogen, because when it is excluded the coefficient r² only drops to 0.84. Spring bloom seems to be an important source of organic carbon pool and biological uptake of oxygen plays a more crucial role in the seasonal evolution of bottom DO than previously thought. Our results indicate that the reassessment phase of the Long Island Sound Total Maximum Daily Load policy on nitrogen loading will most likely fail, because it ignores the contributions of the spring organic carbon pool and river discharge. Also, it is questionable whether the goal of 58.5% anthropogenic nitrogen load reduction is enough.  相似文献   

Response of Nereis diversicolor population (Polychaeta,Nereididae) to the pollution impact – Authie and Seine estuaries (France)     

P. Gillet  M. Mouloud  C. Durou  B. Deutsch 《Estuarine, Coastal and Shelf Science》2008

A survey within the French National Programme of Ecotoxicology was carried out in 2002, 2003 and 2004 to study the response of Nereis diversicolor populations (Polychaeta, Nereididae) to the impact of pollution in the Authie estuary (non-contaminated site) and in the Seine estuary (contaminated site). In the period studied, the density varied from 672 ind. m⁻² to 3584 ind. m⁻² in the Authie estuary and from 80 ind. m⁻² to 920 ind. m⁻² in the Seine estuary. Biomass varied from 3.94 g m⁻² (dry weight) in February 2004 to 38.0 g m⁻² in August 2003 in the Authie estuary and from 3.4 g m⁻² in February 2002 to 0.6 g m⁻² in February 2004 in the Seine estuary. Density and biomass of the populations of N. diversicolor were consistently lower in the Seine estuary than in the Authie estuary. Size frequency histograms permit the analysis of the cohorts as well as the elaboration of the growth curves. For the individuals from the Authie estuary, the relation between dry weight (DW) and length L3 (prostomium, peristomium and chaetiger 1) was DW = 4.2205 L3^2.9832. For those from the Seine estuary, the relation between dry weight and L3 was DW = 0.4697e^1.7209L3. The individuals of N. diversicolor should belong to eight cohorts in Authie estuary (two cohorts each year) instead of six cohorts for those from the Seine estuary. These differences can be attributed to the effect of pollution on the population of N. diversicolor.  相似文献   

Water level variations in the Neuse and Pamlico Estuaries,North Carolina due to local and remote forcing     

Robert E. Reed  David A. Dickey  JoAnn M. Burkholder  Carol A. Kinder  Cavell Brownie 《Estuarine, Coastal and Shelf Science》2008

Water level time series records from the Neuse and Pamlico River Estuaries were statistically compared to local and distant wind field data, water level records within the Pamlico Sound and also coastal ocean sites to determine the relative contribution of each time series to water levels in the Neuse and Pamlico Estuaries. The objectives of this study were to examine these time series data using various statistical methods (i.e. autoregressive, empirical orthogonal function analysis (EOF), exploratory data analysis (EDA)) to determine short- and long-time-scale variability, and to develop predictive statistical models that can be used to estimate past water level fluctuations in both the Neuse Estuary (NE) and Pamlico Estuary (PE). Short- and long-time-scale similarities were observed in all time series of estuarine, Pamlico Sound and subtidal coastal ocean water level and wind component data, due to events (nor'easters, fronts and tropical systems) and seasonality. Empirical orthogonal function analyses revealed a strong coastal ocean and wind field contribution to water level in the NE and PE. Approximately 95% of the variation was captured in the first two EOF components for water level data from the NE, sound and coastal ocean, and 70% for the PE, sound and coastal ocean. Spectral density plots revealed strong diurnal signals in both wind and water level data, and a strong cross correlation and coherency between the NE water level and the North/South wind component. There was good agreement between data and predictions using autoregressive statistical models for the NE (R² = 0.92) and PE (R² = 0.76). These methods also revealed significant autoregressive lags for the NE (days 1 and 3) and for the PE (days 1, 2 and 3). Significant departures from predictions are attributed to local meteorological and hydrological events. The autoregressive techniques showed significant predictive improvement over ordinary least squares methods. The results are considered within the context of providing long time-scale hindcast data for the two estuaries, and the importance of these data for multidisciplinary researchers and managers.  相似文献   

Temporal and spatial changes of dunes in the Changjiang (Yangtze) estuary,China     

Li Weihua  He-Qin Cheng  Li Jiufa  Dong Ping 《Estuarine, Coastal and Shelf Science》2008

The response of subaqueous dunes to the variation of riverine sediment supply in an estuary is rarely reported. Five sets of field measurements of bed sediments and topography were made in the Changjiang estuary, China during dry and flood seasons for the past 9 years. Results show that dunes were mainly three-dimensional (3D) during flood seasons and two-dimensional (2D) dunes during dry seasons. Dunes were also much larger in flood seasons. Dunes mainly occurred within the main channels of the estuary although they extended 11.7 km seaward of their normal extent in the South Channel during dry seasons from 2002 to 2006. This extension of the dune field is likely due to a sharp decline of sediment discharge caused by large reservoirs such as Three Gorges.  相似文献   

Spatiotemporal aspects of silica buffering in restored tidal marshes     

Sander Jacobs  Eric Struyf  Tom Maris  Patrick Meire 《Estuarine, Coastal and Shelf Science》2008

Losses of pelagic diatom production resulting from silica limitation have not only been blamed for toxic algal blooms, but for the reduction in ability of coastal food webs to support higher trophic levels. Recent research has shown the importance of advective seepage water fluxes of dissolved silica (DSi) from freshwater marshes to pelagic waters during moments of riverine Si-limitation. In this study, we investigated the potential impact of recently installed new tidal areas along the Schelde estuary, located in former polder areas and characterized by so-called controlled reduced tidal regimes (CRT). Nine mass-balance studies were conducted in a newly constructed CRT in the freshwater Schelde estuary. During complete tidal cycles both DSi and amorphous silica (ASi) concentrations were monitored at the entrance culverts and in different habitats in the marsh. A swift DSi-delivery capacity was observed despite the shifted spatiotemporal frame of exchange processes compared to reference marshes. As silica-accumulating vegetation is not yet present, and difference with reference marshes’ deliveries is surprisingly small, we indicate diatomaceous debris and phytoliths to be the main silica source. Although further research is necessary on the driving forces of the different processes involved, restoration of former agricultural areas under CRT-regime provide the potential to buffer silica in the estuary.  相似文献   

Nitrification in Kochi backwaters     

Joereen Miranda  K.K. Balachandran  R. Ramesh  Mohideen Wafar 《Estuarine, Coastal and Shelf Science》2008

Nitrification rates, as oxidation of ¹⁵N-labelled ammonium and loss of nitrite from N-Serve treated samples, were measured in Kochi backwaters during three seasons. Nitrification rates ranged from undetectable to 166 nmol N L⁻¹ h⁻¹ in the water column and up to 17 nmol N (g wet wt)⁻¹ h⁻¹ in sediments. Nitrification rates were higher in intermediate salinities than in either freshwater or seawater end. Within this salinity range, nitrification rates could be related to ammonium concentrations. As shown by the relation between ammonification and nitrification rates, it is also likely that nitrification is more regulated by renewal rates, rather than by in situ concentrations, of substrate. Among other environmental parameters, temperature and pH may have an influence on nitrification. Potential nitrification rates calculated from loss of nitrite from N-Serve treated, nitrite-enriched samples were about 800 nmol N L⁻¹ h⁻¹ in the water column and 40 nmol N (g wet wt)⁻¹ h⁻¹ in sediments. While these rates are in balance with those of biological ammonium production they may be inadequate to mitigate ammonium pollution in this estuary.  相似文献   

Reproductive biology and seasonality of the Indo-Australasian mysid Mesopodopsis orientalis (Crustacea: Mysida) in a tropical mangrove estuary,Malaysia     

Yukio Hanamura  Ryon Siow  Phaik-Ean Chee 《Estuarine, Coastal and Shelf Science》2008

A year-round survey of the tropical shallow-water mysid Mesopodopsis orientalis (Tattersall, 1908) (Crustacea, Mysidacea) was conducted in the Merbok mangrove estuary, northwestern Peninsular Malaysia. The mysid formed dense aggregations at the river's edge close to the mangrove forest during the daytime, but very few were captured elsewhere in the estuary system. The sampled population was found in a wide range of salinities from 16 to 32, demonstrating broad euryhalinity, and the number of the catch at the littoral zone ranged from 11.8 to 2273 ind m⁻². The overall annual mean was 709.2 ind m⁻². Females predominated over males in the entire population, and brooding females were present at every monthly sample, indicating that reproduction is continuous year round. The clutch size positively correlated with female body length. The diameter of eggs (Stage I embryos) was unaffected by the seasonality and independent of the maternal size within an observed size range. The life history pattern of the estuarine population of M. orientalis showed close similarity to that of the coastal counterpart. However, the former was found to produce fewer but larger eggs, and the specimens in this population were larger than those in the coastal population at the embryo, juvenile, and adult stages. This evidence indicates that the life history features of the estuarine population would differ to some degree from those of the coastal counterpart.  相似文献   

Silver in Tokyo Bay estuarine waters and Japanese rivers     

Yan Zhang  Hajime Obata  Toshitaka Gamo 《Journal of Oceanography》2008,64(2):259-265

Dissolved and particulate concentrations of silver in Tokyo Bay estuarine waters and Japanese rivers were determined in this study. The dissolved silver concentrations in the surface water of Tokyo Bay range from 5.9 to 15.1 pmol kg⁻¹, which is comparable to those in the surface water of the Japan Sea, but two or three times higher than those in the surface water of the open ocean. However, elevated concentrations of dissolved silver are not found in Tokyo Bay compared with those in other highly urbanized estuaries, such as San Francisco Bay (20∼243 pmol kg⁻¹). In the Tokyo Bay estuary, silver typically exhibits non-conservative mixing behavior, which is a common feature in the other estuaries reported previously. Dissolved silver concentrations decrease with salinity from the rivers to the mouth of Tokyo Bay. Silver is efficiently scavenged by suspended particulates, as evidenced by the high conditional distribution coefficients for silver throughout the estuary (log K_d > 5.0 ± 0.6). The silver fluxes into Tokyo Bay via inflowing rivers and atmospheric deposition were estimated as 83 kg y⁻¹ and 15 kg y⁻¹, respectively. A simple budget calculation shows that the silver supplied from rivers and atmosphere must be rapidly scavenged within the Tokyo Bay estuary.  相似文献