日本虎斑猛水蚤在抑食金球藻中存活、发育和繁殖的实验研究     

周慧欣  王锦秀  王馨  徐子钧  颜天 《海洋科学》2024,48(3):42-49

抑食金球藻(Aureococus anophagefferens)可以形成褐潮, 并对贝类、浮游动物等多种生物均能造成不利影响。为进一步探究抑食金球藻对浮游动物的影响, 本文以日本虎斑猛水蚤(Tigriopus japonicus)为实验生物, 研究抑食金球藻对日本虎斑猛水蚤摄食、存活、生长发育以及繁殖的影响。日本虎斑猛水蚤具有易于在实验室培养、生长周期短、雌雄异体等优点, 是海洋毒性污染物检测的模式生物。实验利用高效液相色谱分析方法, 在日本虎斑猛水蚤体内色素中检测到了抑食金球藻的特征色素19’-丁酰氧基岩藻黄素(But-fuco), 表明日本虎斑猛水蚤能够摄食抑食金球藻。当微藻生物量(相对碳含量)分别同为0.7 μg/mL和7.2 μg/mL时, 在抑食金球藻中无节幼体发育至桡足幼体及成体的存活率均高于以青岛大扁藻为饵料的对照组, 但无显著差异, 并且在前者中无节幼体的发育时间显著低于后者, 12 d内雌体的产卵次数与产卵量显著高于后者。结果表明, 日本虎斑猛水蚤在抑食金球藻中能进行正常的生命活动, 并且是首次报导的一种能够在抑食金球藻中正常摄食、生存、生长发育和繁殖的浮游动物。因此, 当褐潮发生时, 由于贝类幼体等生物会受到显著的不利影响, 日本虎斑猛水蚤等抗性较强的生物可能会成为优势种, 从而会影响浮游动物群落结构的组成, 进而可能会使整个海洋生态系统发生变化。本研究有助于全面了解褐潮对海洋生态系统的影响。  相似文献   

天津近岸海域大型底栖动物摄食功能群特征及其与环境因子的关系   总被引：1，自引：0，他引：1  

徐飞  王军强  蔡文倩  魏静梅  韩雪萌 《海洋与湖沼》2021,52(1):186-195

摄食功能群是指生态系统中食性相同的生物群落,研究摄食功能群有助于科学评估区域生态健康状况.基于2015年9月、2016年5月和2016年9月的天津近岸海域大型底栖动物调查数据,结合同步获取的环境因子,分析研究区大型底栖动物摄食功能群组成及其变化特征,探究大型底栖动物摄食功能群组成及变化的原因.结果表明,3个航次的大型底...  相似文献   

不同饲养方式对乌鳢(Ophiocephalus argus)肠道微生物群落结构差异及种类多样性的影响     

盛鹏程  周冬仁  韩新荣  胡晓波  叶雪平  徐磊  周聃  吴琦芳  郝贵杰 《海洋与湖沼》2020,51(1):148-155

以乌鳢(Ophiocephalus argus)为研究对象,采用不同日粮饲喂,试验W1组饲喂冰鲜鱼,试验W2组饲喂畜禽内脏。12周后,取肠道内容物,利用16S rDNA技术研究投喂不同饲粮的乌鳢肠道生物群落结构差异和微生物多样性,分别获得W1组和W2组乌鳢肠道细菌有效序列51124和39298条。结果分析表明;W1组和W2组物种注释(operational taxonomic unit,OTU)数目分别为2631和4114条,分类地位明确的细菌种类分别隶属5个属和49个属,W1组和W2组乌鳢肠道细菌种类差异很大。W1组乌鳢肠道细菌的优势类群是邻单胞菌属(Plesiomonas)、狭义梭菌属(Clostridium sensu stricto)、鲸蜡菌属(Cetobacterium);W2组乌鳢肠道细菌的优势类群是狭义梭菌属(Clostridium sensu stricto)、邻单胞菌属(Plesiomonas)、Spartobacteria、甲基副球菌属(Methyloparacoccus)、杆菌属(Alsobacter)、Saccharibacteria、分支杆菌属(Mycobacterium)。W2组乌鳢肠道细菌香农指数高于W1组乌鳢肠道细菌,表明前者肠道细菌多样性大于后者。本实验揭示了畜禽内脏饲喂乌鳢肠道细菌的群落种类多样性、OTU丰富度和物种总数均高于冰鲜鱼饲喂乌鳢;W1组肠道有益菌OTU丰富度大于W2组,且W2组还存在一些潜在致病菌。本研究为乌鳢的健康养殖和后续肠道微生物资源的挖掘提供了理论依据。  相似文献   

Feeding intensity and molecular prey identification of the mantis shrimp,Oratosquilla oratoria (De Haan, 1844), in the Tianjin coastal zone of Bohai Bay     

Qi-Kang Bo  Yun-Zhao Lu  Hui-Jing Mi  Yan-Guang Yu  De-Xian Gu  Hong-Zheng You  Shuang Hao 《Marine Ecology》2020,41(4):e12594

Bohai Bay, a representative semi-enclosed bay of China, has been severely impacted in recent decades by rapid human development. Although the mantis shrimp Oratosquilla oratoria plays an important role in the Bohai Bay ecosystem and fishery, its feeding ecology and the impact of habitat changes on its feeding habits are poorly known. In this study, we sought to identify its feeding strategy through the separation of stomach contents, and to describe its trophic ecology during maturation in the Tianjin coastal zone of Bohai Bay by the DNA barcoding method using a fragment (about 690 bp) of the mitochondrial cytochrome oxidase I (CO I) gene. A total of 594 specimens were collected from March to July 2018, and 347 (58.42%) stomachs were found to have food remains. More than half of the O. oratoria individuals had poor feeding activity, and the degree of feeding of females was higher than that of males, but there was no significant difference in the visual fullness index and the fullness weight index (FWI) between sexes for each month. In addition, the feeding activity of O. oratoria was consistent across the months, suggesting in artificial breeding programs it is not worried too much about the accessional diet supplied to the female bloodstocks except maintaining their normal food. A total of 167 retrieved prey items yielded 187 readable sequences; 86.63% of these sequences could be assigned to the species level. Consequently, 25 different prey taxa covering all readable sequences were identified, showing O. oratoria is a generalist predator. In terms of percent composition by frequency of occurrences (%N), crustaceans and fishes accounted for the same and highest percentage (35.83%) in the mantis shrimp diet, followed by mollusks (24.59%) and the remaining marine organisms (3.73%) including Polychaeta (2.67%), Sagittoidea (0.53%), and Chromadorea (0.53%). The result shows the benthic fish Oxudercidae (family) appears to be an especially important food supply for O. oratoria during maturation. The degree of cannibalism decreased with an increase in diet species and the frequencies of cannibalism in this study was noticeably higher than that in previous studies, suggesting that cannibalism may be a significant feeding strategy in the mantis shrimp O. oratoria in the Tianjin coastal zone of Bohai Bay, and because of the frequently cannibalistic behavior, the high stocking density of broodstocks should be avoided.  相似文献   

微塑料对海洋桡足类摄食、排泄及生殖的影响     

于娟  许瑞  魏逾杰  田继远  王雪丹  来敬广  陈容  杨桂朋  张正雨  邵云粹 《中国海洋大学学报(自然科学版)》2020,50(3):73-80

微塑料污染目前成为海洋污染普遍关注的一个研究热点。本文在实验室内将青岛近海常见的海洋桡足类猛水蚤暴露于不同浓度的微塑料尼龙6中,研究了猛水蚤的摄食、排泄以及生殖的变化。研究结果表明,微塑料尼龙6对猛水蚤的摄食、排泄、生殖均产生不利的影响,并且存在剂量-效应关系。微塑料尼龙6对猛水蚤摄食率、滤水率、排粪率的24 h·EC 50分别为67.7、62.2、84.1 mg·L^-1,对猛水蚤抱卵率的144 h·EC 50为30.3 mg·L^-1。“饱食感”造成猛水蚤摄食率降低,从而能量和营养摄入不足可能是导致猛水蚤抱卵率降低的原因。猛水蚤对微塑料的摄食,导致猛水蚤排泄的粪便颗粒小型化,由长椭球体变为短小椭球体,可能与其粘度或物理结构的改变有关。暴露于尼龙6的猛水蚤的粪便体积和沉降速率显著低于未暴露微塑料的对照组。本实验结果对于研究微塑料对海洋桡足类以及滤食性浮游动物的生态毒理影响具有一定的帮助。  相似文献   

Limpets as prey: Lipophrys pholis choice for size     

Ana C. Silva  Ana C. Amador  Sónia Brazão  Claúdia Faria  Diana Boaventura 《Marine Ecology》2010,31(4):525-532

Patellid limpets are key species on rocky shores, structuring intertidal assemblages through their grazing. Their role as prey for fish is, however, often overlooked in studies of intertidal ecosystem functioning. The shanny Lipophrys pholis is a common predator of limpets on rocky shores in Northern Europe and, in this study, we examined the characteristics of its predation on limpets based on prey size. The limpet size range that adult L. pholis (10–12.5 cm) can eat was examined in the laboratory using Patella depressa in 0.2 cm classes, varying between 0.5 cm and 3.0 cm in maximum shell length. There was a limpet size refuge above 1.8 cm, while all smaller sizes were readily consumed by the shanny. The predator attacking behaviour was also examined and found to vary with prey size. Limpets up to 0.8 cm were crushed by the jaw‐constricting force and eaten whole, whereas larger prey were prised from the substratum and the body subsequently separated from the shell. To examine whether there was a L. pholis preference for P. depressa size, a two‐stage laboratory experiment was done using two size classes defined as small (0.7–0.8 cm maximum shell length) and large (1.5–1.6 cm maximum shell length). In the first stage, the predator was given each limpet size class separately. In the second stage, the fish was given a choice between the two classes. Lipophrys pholis was shown to have a preference for the large size class (1.5–1.6 cm). The average number of limpets consumed by the shanny was examined for the duration of one high‐tide typical of the central region of Portugal (≈ 4 h). On average, approximately five limpets (up to an eight limpet maximum) were consumed. The present study shows that L. pholis has a feeding preference based on limpet size, which suggests that the limpet population structure and intra‐ and inter‐specific interactions may be influenced by the shanny predation.  相似文献   

Growth and feeding patterns of European anchovy (Engraulis encrasicolus) early life stages in the Aegean Sea (NE Mediterranean)     

Ignacio A. Catalán  Arild Folkvord  Isabel Palomera  Gemma Quílez-Badía  Fotini Kallianoti  Anastasios Tselepides  Argyris Kallianotis 《Estuarine, Coastal and Shelf Science》2010

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云斑尖塘鳢幼鱼摄食特性的初步研究     

魏成清  陈永乐  朱新平  谢刚  骆豫江  刘毅辉 《广东海洋大学学报》2009,29(6):83-86

研究了云斑尖塘鳢(Oxyeleotris marmorata)幼鱼的摄食特性。结果表明:在幼鱼阶段,云斑尖塘鳢的摄食方式为主动掠食;个体全长10mm的日摄食量约为体重的11.66%,40mm的日摄食量约为体重的51.04%,69mm的日摄食量约为体重的55.30%;日摄食量与幼鱼全长呈正相关关系:A=11.3577+0.7664L(r=0.876);云斑尖塘鳢幼鱼的适口饵料鱼全长为其全长的25%～50%,幼鱼摄食15～17h后可完全消化饵料鱼;云斑尖塘鳢幼鱼昼夜摄食节律明显,一昼夜出现一个摄食高峰,高峰时段为19:00-01:00。  相似文献   

Sensory bases and the mode of feeding behavior of tongue sole <Emphasis Type="Italic">Cynoglossus semilaevis</Emphasis>     

王新安  马爱军 《中国海洋湖沼学报》2009,27(4):708-713

We conducted behavior experiments on the roles of vision, olfaction, and the lateral line in feeding in tongue sole Cynoglossus semilaevis Günter. Chemical cues from the prey alone could not elicit any searching or attacking behavior by the fish; only when moving foods were presented, the chemical nature of the foods affected feeding. In addition, we found that normal tongue sole did not accurately recognize moving artificial prey of different shapes. Neither chemosense nor vision plays an important role in prey detection. Reactions to moving objects and the response of the oropharyngeal cavity to different foods showed that the (eyeless) tongue sole use mainly the lateral line for prey detection and capture. Gustation in the oropharyngeal cavity is essential for the fish to swallow its prey.  相似文献   

Revisiting Peruvian anchovy (Engraulis ringens) trophodynamics provides a new vision of the Humboldt Current system   总被引：6，自引：4，他引：2  

Pepe Espinoza  Arnaud Bertrand   《Progress in Oceanography》2008,79(2-4):215

The Peruvian anchovy or anchoveta (Engraulis ringens) forages on plankton and is a main prey for marine mammals, seabirds, fish, and fishers, and is therefore a key element of the food web in the Humboldt Current system (HCS). Here, we present results from the analysis of 21,203 anchoveta stomach contents sampled during 23 acoustic surveys over the period 1996–2003. Prey items were identified to the genus level, and the relative dietary importance of different prey was assessed by determination of their carbon content. Variability in stomach fullness was examined relative to the diel cycle, the distance from the coast, sea surface temperature, and latitude, using generalized additive models (GAMs). Whereas phytoplankton largely dominated anchoveta diets in terms of numerical abundance and comprised >99% of ingested prey items, the carbon content of prey items indicated that zooplankton was by far the most important dietary component, with euphausiids contributing 67.5% of dietary carbon followed by copepods (26.3%). Stomach fullness data showed that anchoveta feed mainly during daytime between 07h00 and 18h00, although night-time feeding also made a substantial contribution to total food consumption. Stomach fullness also varied with latitude, distance from the coast, and temperature, but with substantial variability indicating a high degree of plasticity in anchoveta feeding behaviour. The results suggest an ecological role for anchoveta that challenges current understanding of its position in the foodweb, the functioning of the HCS, and trophic models of the HCS.  相似文献