海洋青鳉鱼专用型基因芯片的设计及其在生态毒理学上的应用研究   总被引：3，自引：0，他引：3  

陈瑶  方志俊  郑金树  张琦  曾志雄  胡绍燊  杨梦甦 《台湾海峡》2010,29(3):359-366

以生物芯片技术为核心,结合生态毒理学与功能基因组学的方法,制备了海洋青鳉鱼专用型生物芯片,包含180个与细胞分裂、解毒反应、缺氧反应、氧化应激反应、凋亡、生长和发育、性别决定和性腺分化以及生殖激素分泌等功能相关的基因.我们提取了雌性海洋青鳉鱼成体在正常培养和缺氧处理12周后的肝脏和卵巢组织总RNA,利用自制的基因芯片进行了基因表达谱分析,结果显示海洋青鳉鱼在缺氧压力环境下,肝脏和卵巢组织的差异性表达基因分别检测出9个和6个,表明应用此类自制良好的芯片可有效获得海洋青鳉鱼的组织特异性基因表达图谱,也将加强对海洋缺氧状态、氧化应激等反应的分子生物学机制的理解.本研究对于识别异常环境状态下的基因表达模式和组织特异性标志物,进一步开展监测海洋生态毒理因素的研究奠定良好的基础.  相似文献   

海南昌江沿岸海域石珊瑚的物种多样性及其分布     

牛文涛  张潇娴  林荣澄  王初升 《台湾海峡》2010,29(3):389-393

2009年3月,采用水下照相及摄像方法,对海南昌江沿岸海域的石珊瑚物种多样性、分布及覆盖度进行了调查.结合历史资料,共发现石珊瑚10科15属28种和1未定种.其中鹿角珊瑚属（Acropora）、角蜂巢珊瑚属（Favites）、叶状珊瑚属（Lobophyllia）等为优势类群,常见分布珊瑚种类有角孔珊瑚（Gonioporasp.）、标准蜂巢珊瑚（Favia speciosa）、五边角蜂巢珊瑚（Favites pentagona）、多孔鹿角珊瑚（Acropora millepora）、十字牡丹珊瑚（Pavona decussata）、伞房叶状珊瑚（Lobophyllia corymbosa）等.石珊瑚主要分布于海尾镇三联村以南海域,覆盖率最高为12%.本次调查在海南昌江沿岸海域记录的珊瑚种类数多于以前的记录,但少于海南南岸的三亚鹿回头,也少于北部的广西涠洲岛、广东徐闻、香港等海域,随着调查次数的增加,预计会有更多的种类被发现.该海域是北部湾珊瑚幼虫交流的中间过渡区,对珊瑚礁的保护与恢复具有重要意义.  相似文献   

A history of breaking laws—Social dynamics of non-compliance in Vietnamese marine fisheries     

Wiebren J. Boonstra  Nguyen Bach Dang 《Marine Policy》2010

Whether or not fishers comply with regulation depends on the economic and social context in which they operate their vessels. This is how conventional theory explains the phenomenon of non-compliance. It treats state–community interaction processes not as direct causes for non-compliance but rather as background conditions shaping individual fishers’ perception and decisions for action. This paper argues that conventional theory fails to include the dynamics of tempo-relational processes between state and communities, which explains collective patterns of non-compliance in fisheries. The paper addresses this hiatus in the literature, using a process-sociological approach to analyse non-compliance in Vietnamese marine fisheries. The analysis highlights that Vietnamese marine fisheries are mainly regulated through informal networks of trust and mistrust, which function through their interplay with the highly centralised and formalised Vietnamese state. Based on this assessment, the paper concludes that outcomes of processes of the dynamic social interplay between state and communities are semi-dependent on individual perception and action, and as such have a causal effect of their own on patterns of non-compliance in fisheries.  相似文献   

Institutions as facilities for change?: A study of the coherence between political regulations and innovations within the pelagic fisheries sector in Norway     

Stig-Erik Jakobsen  Bernt Aarset 《Marine Policy》2010

Political regulation has traditionally been regarded as antagonistic to innovation, whereas the removal of regulations has been perceived as a precondition for innovations. This paper proposes an alternative position and claim that regulations can have various connections with the innovation process. Regulations can obviously restrict innovations, but they can also facilitate such processes. Systematic empirical research on the relation between political-administrative regulation and innovation has been moderate, and the debate has been highly anecdotal. This article presents an empirical study on how political-administrative regulation within the pelagic fisheries sector in Norway affects innovation. The study confirms that regulations had varied influences on the innovation process, and documents that actor's appraisals of regulations were affected by whether a given regulation is beneficial for the firms and their competitive position or not. Finally, the paper corroborate that the implementation of regulations can promote innovation in firms by providing them with deeper insights into their production processes and to encourage a more intense focus on development and improvement. Implementation of regulations also implies learning, which can increase the firms’ abilities to innovate as well as their insight into the necessity of such practices. The analysis is based on survey data from processors and vessels in the pelagic sector.  相似文献   

大黄鱼肌肉组织cDNA全长文库的构建及EST分析   总被引：1，自引：0，他引：1  

黄伟  薛良义  李婷  杨斌 《台湾海峡》2010,29(2):189-195

以大黄鱼肌肉组织为材料,利用Creator Smart cDNA Library Construction Kit构建了大黄鱼肌肉组织的全长cDNA文库.文库质量分析表明：cDNA文库容量不低于1.68×106cfu/mm3,重组率达96%,插入片断的平均长度大于1 000bp.随机挑取512个cDNA克隆进行5’端测序,其中430条ESTs的长度大于100bp;并初步拼接得到了230个单基因簇（unigene）,其中包括58个重叠群（contigs）,172个单拷贝ESTs（singletons）,冗余度为46.51%.经检索,35个ESTs在BLASTx上无明显的同源性（E值≤1.00×10-10）,为新基因.195个ESTs与已报道的基因有较高的同源性;其中与能量相关基因的ESTs丰度最高,占总数的20.00%.蛋白质合成、细胞骨架和信号传导次之,比例分别为13.48%、12.17%、10.00%,其中包括高迁移率族蛋白B1、瘦素受体、β1-热激蛋白等.这些EST数据为进一步筛选和克隆大黄鱼肌肉特异性表达基因提供了平台.  相似文献   

The Privatization of the Oceans by Rognvaldur Hannesson     

Henry Frew 《The Professional geographer》2007,59(1):156-156

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柴达木盆地3万年左右Ilyocyprisinermis灭绝事件及其古环境意义   总被引：4，自引：0，他引：4  

景民昌  孙镇城  李东明  杨革联  杨平 《现代地质》2001,15(1):49-52

柴达木盆地东南部的达参 1井 4 6 m处 ,介形类 Ilyocyprisinermis突然大量灭绝 ,使得介形类总体丰度大大减少 ,介形类生态组合也发生了明显的变化。该事件称为 Ilyocypris inermis灭绝事件 ,其年限约为 3万 a左右 ,具有十分重要的古环境意义。它是更新世晚期全球气候急剧恶化在柴达木盆地的反映 ,证明了柴达木盆地气候演化和全球气候具有同步性 ;另一方面 ,也为 3万 a左右青藏高原的隆升提供了微古生物方面的证据  相似文献   

海洋产油真菌的简便初筛   总被引：1，自引：0，他引：1  

秦艳红  叶德赞 《台湾海峡》2010,29(1):128-134

选取分离自大洋、深海的28株酵母和143株霉菌，采用在培养基中添加尼罗红比较表观荧光强度的方法来筛选出酵母2株和霉菌7株，但菌体呈红色的酵母（17株）及菌丝为黑色的霉菌（28株）不适用于此法；通过单位DD鲫荧光强度的高低筛选出1株菌落为微红色的酵母，产油性能好于用第一种方法挑选出的2株酵母，且菌体颜色对结果并无影响，但只适合筛选酵母．通过上述2种方法筛选出高油脂菌株，大多来源于深海．  相似文献   

Economic gains of liberalising access to fishing quotas within the European Union     

Jesper L. Andersen  Max Nielsen  Erik Lindebo 《Marine Policy》2009

This paper analyses the extent to which specialisation gains can be achieved by liberalising access to fishing quotas within the European Union (EU). Fishing quotas are today exchanged between EU member states at a rate of 4% of total turnover in EU fisheries. Germany, Belgium, Denmark and the Netherlands are the most active. Only one fourth of these exchanges are permanent. With the management systems in EU fisheries differing among countries, comparative advantages in fisheries exist in member states with the best management practices. Hence, although positive but small specialisation gains exist in EU fisheries today, these gains might potentially be increased by liberalising access to fishing quotas and allowing transferability of quotas between individuals from different countries on a permanent basis. Increasing the gains might, however, affect relative stability.  相似文献   

The infusion of sustainability into bilateral fisheries agreements with developing countries: The European Union example     

Emma Witbooi   《Marine Policy》2008,32(4):669-679

The EU's bilateral fishing policy towards developing third countries has evolved over the years to increasingly emphasise sustainability, culminating in 2002 with the release of a ‘fisheries partnership approach’. The Community has begun to operationalise the policy in various West African coastal states, where its new fisheries partnership agreements are intended to function as ‘development vectors’. This paper introduces the EU's new policy, examines its potential implications for the role of sustainability in future bilateral fishing relations and highlights the impetus for it with reference to analysis of the EU's most recent agreement with Senegal based on empirical research.  相似文献