GeoSOT-3D椭球体剖分真三维数据表达     

孙忠秋  程承旗 《地理信息世界》2016,(3):40-46

随着地球观测技术和信息技术的进步,以全球为背景的科学探索不断增多,不仅是地球表面信息,地下、水下、空中等地球上各个层次的空间信息都受到极大的关注。地球球体剖分网格可以构建真三维的数据模型,进而实现空天地、地下水下一体化的空间数据组织、管理、表达与应用。本文详细论述了GeoSOT-3D椭球体剖分网格的剖分空间框架、剖分编码与剖分尺度特性等理论体系。建立基于GeoSOT-3D的空间对象的真三维表达模型。利用该真三维模型实现了地球椭球体的地上地下可视化,点线面体等空间实体的可视化。研究表明,GeoSOT-3D椭球体剖分具有椭球剖分框架简单、体系完整、二三维一致等特点,因而在该框架下的真三维数据模型可用于下一代地理信息系统中的空间大数据的组织管理与表达。  相似文献   

Morphological and Phylogenetic Studies of a Copepod Species,Irodes parupenei Ho and Lin (2007), Infecting Parupeneus rubescens in Saudi Arabia     

DKHIL Mohamed A  ALHAFIDH Wejdan  AL-QURAISHY Saleh  ALOTAIBI Mashael  BANAEEM Manal  ALSALEH Thekra  ABDEL-GABER Rewaida 《中国海洋大学学报(英文版)》2022,21(2):457-464

Dammam City is one of the gorgeous coastal areas in the Arabian Gulf of Saudi Arabia.The present study aimed to ex-amine one of the copepod species infecting the rosy goatfish that represents a highly consumed fish species by the local population in the Arabian Gulf.The copepod species isolated from the infected fish specimens belong to the family Taeniacanthidae and was iden-tified as Irodes parupenei Ho and Lin(2007),primarily based on its morphological,morphometric,and ultrastructural characteris-tics,especially the structures of the dorsal cephalic area,segmentation of the first antenna,the absence of the maxilliped claw in the fe-male specimens,and the setation and spinulation of the legs 2-4 for the adult females are of great significance in the taxonomic iden-tification.The 18S rRNA gene sequence was analyzed to ensure the precise identity and exact taxonomic status of the copepod species.The result showed that this copepod species belong to Taenicanthidae and closely related to Irodes sauridi(gb|JF781550.1)in the same taxon.More details on the specificity of the goatfish for Irodes species and identifying these parasitic taxa using molecular analysis are given in the present study.  相似文献   

A Sensitive and Versatile Multiplex PCR System for the Rapid Detection of Enterotoxigenic (ETEC), Enterohaemorrhagic (EHEC) and Enteropathogenic (EPEC) Strains of Escherichia coli     

R.Y.C Kong  C.L So  W.F Law  R.S.S Wu 《Marine pollution bulletin》1999,38(12):21-1215

Although Escherichia coli is widely distributed in the marine environment, only a small percentage are pathogenic to humans. Nonetheless, the widespread occurrence of waterborne infections of E. coli origin in humans has become one of the major health problems worldwide. To date, several types of enterovirulent E. coli have been recognized as the aetiologic agents of various gastrointestinal infections in humans. The most commonly encountered are those belonging to the enterotoxigenic (ETEC), enteroinvasive (EIEC), enterohaemorrhagic (EHEC) and enteropathogenic (EPEC) subtypes. In order to better determine the health risks that are associated with exposure to some of these specific subtypes, we have developed a very sensitive multiplex PCR system for the rapid detection and typing of ETEC, EHEC and possibly EPEC strains of E. coli in the aquatic environment. The target genes chosen for this investigation included: the PHO-A housekeeping gene (present in all E. coli); the LT1, LT2 and ST1 genes of ETEC; the VT1 and VT2 verotoxin, and EAE virulence genes of EHEC and EPEC, respectively. Six pairs of oligonucleotide primers were designed to simultaneously amplify internal fragments of these genes by multiplex PCR to generate PCR products that could be analysed and confirmed with relative ease by gel electrophoresis and HincII enzyme digestion. The results showed that the six sets of PCR primers were highly specific for their target genes and produced specific amplimers of the expected size from several control strains of E. coli – ATCC 35401 (LT1+/ST1+); SA53 (LT2+/VT2+); and O157 (VT1+/VT2+/EAE+). The detection sensitivity of the multiplex PCR system for the six target genes in an E. coli cell mixture was optimized and enhanced by preincubating serially diluted cells in Luria-Bertani broth for 6 h prior to PCR analysis. The results obtained indicated a detection sensitivity of 10° CFU (of each strain) per 100 μl reaction. Multiplex PCR analysis of seawater samples collected from four sewage-polluted sites in Hong Kong indicated the presence in all four samples of E. coli bacteria that were positive for LT1, ST1, VT1 and EAE virulence genes. Overall, the data indicated that the multiplex PCR system described in this study is a potentially very useful and powerful method for routine monitoring and risk assessment of water quality.  相似文献   

A Modified Form of Mild-Slope Equation with Weakly Nonlinear Effect   总被引：6，自引：0，他引：6  

LI Ruijie  WANG Houjie Dr.  Associate Professor  Engineering College of Ocean University of Qingdao  Qingdao  P. R. China Ph. D. Candidate  Engineering College of Ocean University of Qingdao  Qingdao  P. R. China 《中国海洋工程》1999,(3)

Nonlinear effect is of importance to waves propagating from deep water to shallow water.Thenon-linearity of waves is widely discussed due to its high precision in application.But there are still someproblems in dealing with the nonlinear waves in practice.In this paper,a modified form of mild-slope equa-tion with weakly nonlinear effect is derived by use of the nonlinear dispersion relation and the steady mild-slope equation containing energy dissipation.The modified form of mild-slope equation is convenient to solvenonlinear effect of waves.The model is tested against the laboratory measurement for the case of a submergedelliptical shoal on a slope beach given by Berkhoff et al,The present numerical results are also comparedwith those obtained through linear wave theory.Better agreement is obtained as the modified mild-slope e-quation is employed.And the modified mild-slope equation can reasonably simulate the weakly nonlinear ef-fect of wave propagation from deep water to coast.  相似文献   

狄氏斧蛤(Donax dysoni)线粒体全基因组测定及结构特征分析          下载免费PDF全文

马颜雯  李继姬  叶莹莹 《海洋与湖沼》2023,54(1):246-258

为解析狄氏斧蛤(Donax dysoni)线粒体全基因组结构特征以及进化地位,采用二代高通量测序技术获得狄氏斧蛤线粒体基因组全序列,对线粒体基因进行注释并对其序列结构进行分析。结果表明,狄氏斧蛤的线粒体全基因组序列全长为16 908 bp,碱基组成为A (26.81%)、T (41.13%)、G(21.21%)和C (10.85%),A+T含量为67.94%,表现出明显的AT偏向。与其他双壳贝类相似,狄氏斧蛤共编码13个蛋白质编码基因,包含22个t RNA和2个r RNA,且所有基因均位于H链;蛋白质编码基因拥有3种起始密码子(ATG、ATT、ATA),而除了ND3、ND4以及ATP6使用TAG作为终止密码子外,其余所有蛋白质编码基因都使用TAA作为终止密码子。除t RNASer不能折叠成典型的三叶草结构,没有明显的DHU茎环,其余t RNA都能折叠成典型的三叶草结构。狄氏斧蛤与斧蛤科(Donacidae)其他物种具有相同的基因排列,未发现基因重排现象。基于线粒体12个蛋白质编码基因构建62种贝类的进化树,结果显示:Donax semiestriatus和Donax vittatus聚...  相似文献   

双壳贝类CK1基因家族的鉴定及表达分析     

孙凤芝  吕珍立  刘福云  邢强  连姗姗  王师  包立随 《海洋与湖沼》2023,54(5):1401-1412

酪蛋白激酶1 (CK1)是一种重要的蛋白激酶家族,其在DNA损伤应答和修复、细胞增殖和凋亡、胚胎发育和稳态等重要的生物学过程中都具有复杂多样的调控作用。为了理解CK1基因家族在双壳贝类中的特征、进化及生物学功能,实验采用比较基因组学及生物信息学的方法对双壳贝类CK1基因家族进行了鉴定分析,通过虾夷扇贝高温应激实验,研究了CK1基因家族在高温应激时的表达规律。结果显示,在虾夷扇贝、栉孔扇贝、长牡蛎与侏儒蛤中均存在CK1α,CK1αlike,CK1δ,CK1γ3,并发现CK1ε,CK1γ1,CK1γ2在双壳贝类中发生了丢失。时空表达分析发现,双壳贝类CK1基因均在胚胎发育早期集中表达,并以多细胞/囊胚期为界呈现出母源/合子特异性表达模式。CK1为关键基因的基因共表达模块显著富集在错配修复、核苷酸剪切修复等相关通路上,暗示了CK1基因在双壳贝类胚胎发育过程中参与调控DNA损伤修复过程,从而维持早期胚胎发育时的基因组稳定性。双壳贝类CK1基因在成体中呈现组织特异的表达模式,主要在鳃和雄性性腺中具有相对较高的表达量。虾夷扇贝受到高温应激后,其鳃中PyCK1α, PyCK1αlike和PyCK1δ...  相似文献   

鲨肝刺激物质类似物基因在大肠杆菌中的表达与产物活性分析   总被引：2，自引：0，他引：2  

王颖  边杉  张婷婷  赵艳景  吴梧桐  叶波平  王旻 《海洋科学》2004,28(6):37-41

构建了鲨肝刺激物质类似物基因片段的原核表达载体质粒 (pET-sHSS) ,转化大肠杆菌后通过IPTG诱导获得原核表达产物 ,并利用凝胶层析方法对表达产物进行了分离纯化 ;进一步利用MTT比色法研究了该重组产物对肝癌细胞株SMMC -7721的增殖影响。结果表明 ,在1mmol/L的IPTG的诱导下 ,鲨肝刺激物质类似物基因片段在大肠杆菌BL21菌株中获得表达 ,表达产物的相对分子质量大小约为17500u,占菌体可溶性蛋白总量的38%左右 ;纯化后的产物在低浓度下 (<50ng/L)具有明显刺激SMMC7721细胞株增殖的活性 ,高浓度下 (>100ng/L)则明显抑制该肿瘤细胞株的生长 ,与天然鲨肝刺激物质的生物活性类似  相似文献   

空间地理信息组织表达方法探讨     

李晓轩  王光霞  李晓  刘毅  李志远 《测绘科学》2010,35(3):140-142

随着空间地理信息以及各种新型地图产品在各个领域的广泛应用,人们发现不同时期,应用需求对空间地理信息组织表达的要求是不同的,不同领域和部门对地理信息的组织表达方式也是不同的。但是,目前空间地理信息的共享和互操作、空间地理信息的多尺度分析与表达等,都要求空间地理信息的组织与表达要科学、合理和规范,否则难以满足测绘信息服务化及信息化测绘体系的建设。本文以交通类信息组织表达为例,详尽阐述当前空间地理信息组织表达的现状,分析其原因,并提出了解决这一问题的思路和方法,为今后信息化测绘体系建设、数据库数据组织和地理信息表达,提供理论和方法借鉴。  相似文献   

基于GML3.2的对象化空间数据组织与关系表达研究   总被引：1，自引：0，他引：1  

梁汝鹏  李宏伟  李文娟  孟凡琏 《测绘科学》2010,35(2):102-105

数据组织是GIS系统建设的基础和前提,随着对地理世界认知的不断深入,逐渐形成了基于特征的空间数据模型。以基于特征的空间数据组织基本理论为基础,文章主要讨论基于GML3.2的空间数据组织与关系表达的关键问题。首先,简要介绍GML的基本概念,并详细阐述GML3.2的主要模式。在此基础上,讨论在GML应用模式设计中需要注意的相关原则。最后,总结探讨了实现基于GML3.2的对象化空间数据组织与关系表达的途径。  相似文献   

水体细菌碱性磷酸酶及其编码基因研究进展   总被引：1，自引：0，他引：1  

戴江玉  高光  吴时强  吴修锋  周杰  薛万云  杨倩倩  陈丹 《湖泊科学》2016,28(6):1153-1166

水体溶解性反应磷是浮游植物可直接利用的主要磷营养形式,细菌碱性磷酸酶催化的有机磷矿化过程则是维持溶解性反应磷供给的重要途径.通过总结细菌碱性磷酸酶的种类与分布特征,发现主要包括Pho A、Pho X和Pho D 3种类型细菌碱性磷酸酶,其中超过50%的Pho X分布于细菌细胞的外部环境,对溶解性反应磷的循环再生尤为重要.综述水体产碱性磷酸酶细菌群落组成的研究进展,以及影响编码基因表达的环境因素,对比分析产碱性磷酸酶细菌群落鉴定方法的特点及发展趋势.细菌碱性磷酸酶编码基因的研究多关注寡营养海洋生态系统,对于同样存在磷限制问题的富营养化湖泊生态系统鲜有涉及,而构建针对典型富营养化湖泊生态系统的细菌宏基因组数据库,则可为建立淡水生态系统细菌碱性磷酸酶及其编码基因的研究方法奠定基础,有助于认识湖泊水华频发的微生物学驱动机制.  相似文献