一株菌红素产生菌的鉴定及色素组分分析     

崔恒林  代春华  董英  陆玲  秦怀兰 《海洋科学》2005,29(2):43-48

对分离自腌鱼的高产菌红素(bacterioruberin)的菌株r-fish进行了形态特征、生理生化特性与细胞化学组分的分析以及基于16SrDNA序列的系统发育分析。形态特征、生理生化特性及细胞化学组分的分析结果表明菌株r-fish为球形的嗜盐古菌。基于16SrDNA序列的系统发育结果表明,r-fish与Halobacterium trapanicum NCIMB784菌株的相似性为99．8％,归入Na-trinema属。用TLC法对r-fish的色素进行了分析,r-fish的色素组分包括菌红素(bacteriorubefin)、脱水菌红素(anhydrobacteriorubefin)、双脱水菌红素(bisanhydrobacteriorubeftn)和一未知色素。与Halobacteriumhalobium和Halococcus morrhuae的色素组分相似。  相似文献   

Sedimentary dynamics and ecological state of Nakta tidal flat (littoral), South of Sfax, Gulf of Gabès, Tunisia     

Gargouri-Ben Ayed Z.  Souissi R.  Soussi M.  Abdeljaouad S.  and Zouari K. Laboratoire de radio-analyses et environnement  Ecole Nationale d’Ingénieurs de Sfax  BP W  Sfax  Tunisie Université Tunis El Manar  Faculté des Sciences de Tunis  Département de géologie    Tunis  Tunisie 《中国地球化学学报》2007,26(3):244-251

The tidal flat of Nakta is located in the northern part of the gulf of Gabes and in the southern coast of Sfax. It corresponds to a flat reef protected from less topography, with a slope ranging between 2° and 4°, which borders the Gargour Wadi. The study sector is renowned to have moderate hydrodynamics which lasts almost for two millenary (14C isotopic dating). The sedimentological study of the Nakta tidal flat revealed different facies: fine-grained sand in the intertidal zone and carbonated muddy sand in the infratidal zone. Equilibrium state of the Nakta ecosystem depends entirely on tide currents, which mainly inhibit drift currents. The Nakta tidal flat is characterized by a variety of faunal species (Cerastoderma glaucum, Arca noe, Cardita antiquatus, Chlamys varied, Ruditapes deccussatus, Tapes rhomboids, Pinctada radiate, etc.) and floristic diversities (Halocnemum strobilacum, Halimione portulacoides, Enteromorpha linza, Ulva rigida, Cymodocea nodosa, Posidonia oceanica). The species are abundant in the infratidal zone, while in its intertidal zone, faunal species remain little diversified and are dominated by limivorous diggering. The paleogeographic study of the Nakta tidal flat showed the alternation of regression and transgression periods.  相似文献   

我国近海中度嗜盐菌的分离筛选及其产酶多样性分析   总被引：1，自引：0，他引：1  

产竹华  刘洋  王昭凯  钟添华  曾润颖 《海洋学报》2014,36(2):115-122

为了解我国近海中度嗜盐菌系统发育多样性及产酶特性,从天津、山东、江苏、福建、海南等地近海采集样品为研究对象,分离筛选得到108株中度嗜盐菌,其中有26株至少产一种酶。通过对其形态特征、生理生化、16SrRNA基因序列及系统进化树进行分析,将26株菌鉴定为细菌域的Halomonas、Idiomarina、Virgibacillus、Pontibacillus、Oceanobacillus、Halobacillus和Marinilactibacillus属的菌株。它们与相应属的模式菌株16SrRNA基因序列相似性在97%~100%不等,其中有些菌株可能代表不同的分类单元。底物特异性试验表明,分离的26株中度嗜盐菌13株产蛋白酶,19株产淀粉酶,13株产酯酶,4株产纤维素酶。其中6株产3种酶,11株产2种酶。研究结果表明中度嗜盐菌具有系统发育和产酶多样性,同时蕴藏着较多新的微生物类群,为嗜盐微生物资源应用开发提供了参考。  相似文献   

Biodiversity and Screening of Moderately Halophilic Bacteria with Hydrolytic and Antimicrobial Activities from Yuncheng Salt Lake,China     

LI Xin  YU Huiying 《《地质学报》英文版》2014,88(Z1):84-85

正1 Introduction Yuncheng Salt Lake is an old lake with a history of more than 5000 years,which locates in the margins of the central plains of China,with an area of about 130 km.It was an important resource of salt for human consumption from early times.Major chemicals in Yuncheng Salt Lake contain Na Cl,Na2SO4 and Mg SO4(Gao et al.2007).In recent years,some halophilic bacteria and archaea were  相似文献   

烟台海域中度嗜盐菌分离、鉴定和系统进化研究   总被引：1，自引：0，他引：1  

冯二梅  宿红艳  王磊 《海洋通报》2010,29(1)

为研究山东烟台海域中度嗜盐菌的分布情况及种群特征,从烟台近海盐场采集的土样和水样中利用高氏一号培养基分离得到22株中度嗜盐菌。通过菌落形态观察、生理生化特征分析比较,选取其中的9株进行16SrDNA序列分析和系统进化分析,结果表明这些中度嗜盐菌分别属于7个属:芽孢杆菌属(Bacillus)、枝芽孢杆菌属(Virgibacillus)、薄壁芽孢杆菌属(Gracilibacillus)、刘氏菌属(Liuella)、产微球茎菌属(Microbulbifer)、拟诺卡氏菌属(Nocardiopsis)和交替单胞菌属(Alteromonas),其中菌株YTM-8、YTM-12和YTM-13可能是潜在的新种。以上研究结果表明烟台海域中存在有较为丰富的嗜盐微生物多样性,并且潜藏着新类型的嗜盐菌资源。  相似文献