罗氏沼虾(Macrobrachium rosenbergii)传染性皮下和造血器官坏死病毒(IHHNV)的流行病学调查   总被引：1，自引：0，他引：1  

范东东  魏永伟  苗亮  陈炯 《海洋与湖沼》2015,46(5):1153-1159

传染性皮下和造血器官坏死病毒(infectious hypodermal and hematopoietic necrosis virus,IHHNV)是对虾养殖的重要病原之一,可感染多种虾种。罗氏沼虾(Macrobrachium rosenbergii)是中国养殖的重要沼虾品种之一。根据国际兽疫局(Office International des Epizooties,OIE)推荐的IHHNV检测方法,在中国大陆地区首次开展IHHNV在罗氏沼虾中的感染和流行情况调查。结果显示,在研究区的罗氏沼虾养殖区,IHHNV广泛流行,阳性率高达90%;但所有成年罗氏沼虾均未表现出明显的病症,仅表现为病毒的携带。通过基因序列分析显示,检测到的华南地区毒株属于Ⅰ型感染株,与菲律宾株进化关系较为接近;华东地区毒株属于Ⅱ型感染株,与东南亚株进化关系较近。本研究为IHHNV在罗氏沼虾内的感染、流行和防控提供了详细参考。  相似文献   

海洋解淀粉芽孢杆菌(Bacillus amyloliquefacien)产抗生素Macrolactin A的碳源优化     

侯东园  吴祖芳  张鑫 《海洋与湖沼》2015,46(5):1071-1077

为提高一株海洋解淀粉芽孢杆菌(Bacillus amyloliquefaciens)ESB-2生产大环内酯类抗生素Macrolactin A的能力,利用大孔树脂对发酵液进行粗提取,利用高速逆流色谱法,高效、快速分离制备出高纯度的Macrolactin A。采用高效液相色谱法检测不同碳源对Macrolactin A产量的影响,且在优化基础上采用摇瓶发酵得到海洋解淀粉芽孢杆菌的发酵过程曲线。结果表明:高速逆流色谱法能够高效快速的制备出Macrolactin A纯品,纯度达到95%以上,明显优于高效液相色谱;添加葡萄糖、蔗糖和麦芽糖均有利于Macrolactin A的生成,麦芽糖为最佳碳源,最佳浓度为1%,其产量达到18.5mg/L,是优化前的2倍多。本研究可为提高Macrolactin A的产量、解决其药源问题、实现规模化生产打下了良好的基础。  相似文献   

High connectivity and directional gene flow in European Atlantic and Mediterranean populations of Ciona intestinalis sp. A          下载免费PDF全文

Ornella Affinito  Nikos Andreakis  Luigi Caputi  Rita Marino  Raimondo Pannone  Paolo Sordino  Gabriele Procaccini 《Marine Ecology》2015,36(4):1230-1243

Understanding the factors that cause population divergence has long been of interest to marine biologists in their attempts to interpret the effect of human‐mediated vectors. Broadcast‐spawning species with limited dispersal capability are excellent candidates to measure the present‐day patterns of genetic diversity. The tunicate Ciona intestinalis (Ascidiacea) is comprised of a complex of morphologically cryptic species that form vigorous aggregates in eutrophic habitats (harbors, gulfs and lagoons) where they can compete with the epibenthic community and cause biofouling problems. This study investigated biogeographic variability and migration patterns of C. intestinalis sp. A along Northeast Atlantic and Mediterranean coasts using microsatellite markers. Data presented here on 371 specimens collected from 17 populations reveal high genetic polymorphism, but with a deficit of heterozygote deficiency. Absence of evidence for isolation by distance suggests that the genetic patterns do not reflect the geographic distribution of sampled populations. Substantial gene flow and artificial potential for dispersal boost high levels of within‐population genetic variability and prevent genetic differentiation within and between seas. A predominant eastward migration pattern was revealed by the data set, with very limited opportunity for C. intestinalis sp. A to travel westward. This directional movement indicates that other properties (e.g. habitat quality, genetic traits, mating system, life cycle) may cause adaptive divergence at a large biogeographic scale.  相似文献   

Species delimitation in the green algal genus Codium (Bryopsidales) from Korea using DNA barcoding     

LEE Hyung Woo  KIM Myung Sook 《海洋学报(英文版)》2015,34(4):114-124

Codium, one of the largest marine green algal genera, is difficult to delimit species boundary accurately based on morphological identification only. DNA barcoding is a powerful tool for discriminating species of seaweeds. The plastid elongation factor TU(tuf A) is considered as maker to perform DNA barcoding of green algal species than rbc L gene due to universality and rapid evolution rate. We conducted DNA barcoding application to Codium specimens from the Jeju Island, Korea to overcome the limit of morphological identification and to confirm the species diversity. As a result of applying tuf A marker, we newly generated fifty-five tuf A barcodes to resolve eight species. Tuf A marker exhibited 6.1%–21.8% interspecific divergences, wider than the gap of rbc L exon 1,3.5%–11.5%. Molecular analysis of rbc L exon 1 sequences of Codium revealed eight distinct species like tuf A analysis separated in five phylogenetic groups. DNA barcoding of the genus Codium using tuf A marker is more helpful to overcome the limit of morphological identification, and this is more potential to reveal cryptic species and to resolve the relationships among subspecies than rbc L analysis alone. The complement of tuf A barcoding and rbc L analyses including morphology for the genus Codium in the northwestern Pacific will give much more reliable achievement for discovering species diversity and resolving the phylogenetic relationships.  相似文献   

HY-2A卫星雷达高度计数据的全球统计评价及质量分析   总被引：6，自引：4，他引：2  

彭海龙  林明森  穆博  周武 《海洋学报》2015,37(7):54-66

自HY-2A卫星发射以来,针对HY-2A卫星雷达高度计产品的交叉定标、真实性检验及质量评估工作一直在持续开展。本文主要以HY-2A卫星高度计第44周期的IGDR产品数据为例,通过使用全球分布图、二维直方图和每日均值统计的方法完成了与Jason-2IGDR产品的比对验证,同时对主要环境校正参数及地球物理产品的数据质量稳定性进行了分析,结果显示高度计产品数据质量较稳定,此外利用HY-2A卫星升降轨交叉点海面高度差、与Jason-2卫星交叉点海面高度差以及沿轨海平面异常数据分析的方法进行了HY-2A卫星高度计观测系统的性能评估,结果显示,HY-2A卫星海面高度精度约为7.48cm,精度接近Jason-2,能满足海洋应用与科学研究的需要。  相似文献   

基于HJ-1A高光谱的黄河口碱蓬和柽柳盖度反演模型研究   总被引：3，自引：2，他引：1  

任广波  张杰  马毅 《海洋学报》2015,37(9):51-58

碱蓬和柽柳是黄河口湿地典型的盐生植物类型,是多种保护珍禽的主要栖息地,具有景观尺度较小、分布广且多混生的特点。应用覆盖黄河口北部潮滩的HJ-1A高光谱遥感影像,基于现场测量的端元光谱和从遥感影像中使用顺序最大角凸锥法(SAMCC)自动提取的端元光谱,应用线性光谱分解法(LSU)、正交子空间投影法(OSP)、匹配滤波法(MF)、最小能量约束法(CEM)和自适应一致估计法(ACE)5种不同光谱解混方法进行混合像元光谱解混,对比两种方法得到的端元光谱分别对碱蓬和柽柳盖度的反演能力,并给出相应的反演模型。结果显示:(1)现场测量端元光谱取得了较好的碱蓬和柽柳盖度反演结果,其中应用LSU方法的光谱解混结果与现场测量盖度的决定系数对于碱蓬和柽柳分别达到了0.88和0.95;(2)两种端元获取方式的光谱解混结果中,LSU和OSP方法均获得了较高的相关性,ACE解混方法的相关性都最低;(3)SAMCC方法提取端元光谱对柽柳的分解结果与现场测量盖度的相关性远高于碱蓬。  相似文献   

SRES A2 情景下中国区域性高温热浪事件变化特征   总被引：3，自引：0，他引：3  

杨红龙  潘婕  张镭 《气象与环境学报》2015,31(1):51-59

随全球变暖,应对高温热浪事件是未来现代化城市面临的难题之一。本文利用全球模式-HadAM3p提供的3组不同边界场和初始场驱动区域气候模式系统PRECIS的输出结果,模拟未来情景下中国区域性高温热浪事件发生频率、强度及持续时间的变化趋势。结果表明：全球PRECIS对基准时段（1961-1990年）的高温热浪事件的发生的频率、强度和持续时间及对应的大气环流特征具有较强的模拟能力。相对于基准时段,未来情景下未来时段（2071-2100年）中国各地区的高温热浪的强度增加,发生频率增幅超过了100 %,且持续时间增加30 %以上。此外,观测资料和模拟结果均表明武汉和哈尔滨地区的高温热浪与500 hPa高度场的正距平密切相关。而未来情景下,武汉和哈尔滨地区500 hPa高度场的正距平呈增加趋势,表明这些地区未来可能面临危害更严重的高温热浪事件。  相似文献   

Diagnosis of nervous necrosis virus in orange-spotted grouper, Epinephelus coioides, by a rapid and convenient RT-PCR method     

MU Yinnan  LIN Kebing  CHEN Xinhu  AO Jingqun 《海洋学报(英文版)》2013,32(10):88-92

  相似文献   

First six months quality assessment of HY-2A SCAT wind products using in situ measurements   总被引：4，自引：3，他引：1  

WANG He  ZHU Jianhu  LIN Mingsen  HUANG Xiaoqi  ZHAO Yili  CHEN Chuntao  ZHANG Youguang  PENG Hailong 《海洋学报(英文版)》2013,32(11):27-33

The first Chinese microwave ocean environment satellite HY-2A, carrying a Ku-band scatteromenter （SCAT）, was successfully launched in August 2011. The first quality assessment of HY-2A SCAT wind products is presented through the comparison of the first 6 months operationally released SCAT products with in situ data. The in situ winds from the National Data Buoy Center （NDBC） buoys, R/V Polarstern, Aurora Australis, Roger Revelle and PY30-1 oil platform, were converted to the 10 m equivalent neutral winds. The temporal and spatial differences between the HY-2A SCAT and the in situ observations were limited to less than 5 min and 12.5 km. For HY-2A SCAT wind speed products, the comparison and analysis using the NDBC buoys yield a bias of-0.49 m/s, a root mean square error （RMSE） of 1.3 m/s and an increase negative bias with increasing wind speed observation above 3 m/s. Although less accurate of HY-2A SCAT wind direction at low winds, the RMSE of 19.19° with a bias of 0.92° is found for wind speeds higher than 3 m/s. These results are found consistent with those from R/Vs and oil platform comparisons. Moreover, the NDBC buoy comparison results also suggest that the accuracy of HY-2A SCAT winds is consistent over the first half year of 2012. The encouraging assessment results over the first 6 months show that wind products from HY-2A SCAT will be useful for scientific community.  相似文献   

基于ARM7的嵌入式三维电阻率采集子站的设计研究     

蒋培荡  龚育龄  钟念兵 《铀矿地质》2010,26(6):376-379

提出了一种基于ARM7的嵌入式三维电阻率采集子站设计方法,采用24位A/D转换器（ADS1258）和ARM7微处理器（LPC2378）,使三维电阻率法数据采集系统具有体积小、功耗低、成本低、精度高等特点,同时实现μC/OSⅡ操作系统在ARM的移植,为数据采集的实时性提供可能。  相似文献