螺旋藻多糖对CD₃AK细胞增殖能力的影响   总被引：7，自引：0，他引：7  

王友顺  黎露刚  吴侃  谢宗华  唐庆  李新岗  祁铁流  李长亮  汪洋  汪庭  方光如 《海洋科学》1998,22(3):37-39

研究了螺旋藻多糖（ＰＳ）对ＣＤ３ＭｃＡｂ激活的杀伤细胞（ＣＤ３ＭｃＡｂ Ａｃｔｉｖａｔｅｄ Ｋｉｌｌｅｒ Ｃｅｌｌｓ,ＣＤ３ＡＫ Ｃｅｌｌｓ）增殖能力的影响。结果表明,当ＰＳ浓度为２．５μｇ·ｍｌ＾－１培养体系条件下,对ＣＤ３ＡＫ细胞具有明显的刺激细胞增殖作用（Ｐ〈０．０２）;对培养长达２３ｄ的ＣＤ３ＡＫ细胞杀伤肿瘤细胞（Ｋ５６２细胞）的活性仍维持在较高的水平（４６．５％￣５０％）。提示ＰＳ对辐射  相似文献   

环境因子对三种常见微藻细胞中二甲基硫丙酸含量影响的初步研究   总被引：2，自引：0，他引：2  

李炜  焦念志 《海洋与湖沼》1999,30(6):635-639

以3种常见的海洋浮游藻类──扁藻、杜氏藻和牟氏角刺藻作为实验材料,采用正交方法设计实验条件,研究盐度、温度和光强的变化对藻类细胞内二甲基硫丙酸（DMSP）含量的影响。结果表明,3种藻类细胞DMP含量相差很大。扁藻DMSP含量最高,其次为杜氏藻,牟氏角刺藻含量最低。种间差别的影响明显高于环境条件的变化对藻细胞DMSP含量的影响。3种环境因子对藻细胞DMSP含量的影响效果不同,盐度变化引起藻细胞DMP含量的变化最为显著,其次为温度和光强。随着盐度的增加,扁藻、杜氏藻和牟氏角刺藻细胞内DMSP含量均呈上升趋势。  相似文献   

Cascades of sub-decadal,channel-floodplain changes in low-gradient,non-vegetated reaches near a dryland river terminus: Salar de Uyuni,Bolivia     

Jiaguang Li  Stephen Tooth  Guangqing Yao 《地球表面变化过程与地形》2019,44(2):490-506

The terminus of the ephemeral Río Colorado is located at the margins of Salar de Uyuni, Bolivia, the world's largest salt lake. The low-gradient (<0.0006 m m^-1), non-vegetated reaches approaching the terminus provide an excellent natural laboratory for investigating cascades of channel-floodplain changes that occur in response to quasi-regular flows (at least once annually) and fine-grained sediment supply (dominantly silt and clay). High-resolution satellite imagery (<0.65 m, various dates from 2004 onwards) and field data reveal widespread, pronounced and rapid morphodynamics on sub-decadal timescales, including channel erosion and chute cutoff formation, and development of crevasse channels and splays, floodouts (unchannelled surfaces at channel termini), and erosion cells (floodplain scour-transport-fill features). In particular, following high annual precipitation (>400 mm) in 2004–2005 and two subsequent high magnitude daily precipitation events (~40 mm), all of which led to widespread flooding, numerous crevasse splays formed between 2004 and 2016, avulsions occurred at nearby floodouts, and erosion cells downstream of the splays and floodouts underwent striking morphological changes. High-precision GPS data reveal two preferential localities for erosion cell development: partially or fully abandoned channels with crevasse splay remnants, and topographic lows between channels. In this overall low-gradient setting, comparatively high gradients (up to ~0.0006 m m^-1) at the edge of splay deposits and topography created by crevasses and abandoned channels may initiate knickpoint retreat and thereafter erosion cell development. Abandoned channels with splays tend to give rise to narrow, deep erosion cells, while topographic lows promote relatively shallow, wide erosion cells. In both situations, erosion cells may extend upslope and downslope, and eventually connect to form straight channels. The channel-floodplain morphodynamics near the Río Colorado terminus extend previous analyses of low-gradient, dryland river systems, particularly because the lack of vegetation and quasi-regular floods drive cascades of rapid changes on sub-decadal timescales. © 2018 John Wiley & Sons, Ltd.  相似文献   

日本七鳃鳗外周血细胞显微结构及类淋巴细胞体外培养   总被引：1，自引：0，他引：1  

逄越  李庆伟 《海洋科学》2012,36(1):23-29

以日本七鳃鳗(Lampetra japonica)为研究对象,观察其外周血细胞显微结构和探讨类淋巴细胞原代培养条件。采用Wright氏和Giemsa氏染色法对日本七鳃鳗外周血液有形成分进行显微观察,可鉴别出红细胞、淋巴细胞、中性粒细胞、单核细胞、嗜酸性粒细胞;未发现嗜碱性粒细胞。通过Ficoll密度梯度离心法和流式细胞仪分选获得高纯度的七鳃鳗类淋巴细胞。经条件优化后确定最适培养液L15+20%FBS(5%七鳃鳗血清)+0.46%NaCl,在18℃,pH 6.8-7.0的条件下对日本七鳃鳗类淋巴细胞进行体外培养,大多数类淋巴细胞半贴壁生长,细胞状态较好,最长可以存活近一个月。类淋巴细胞的原代培养为日本七鳃鳗细胞体外培养的深入研究提供了实验依据。  相似文献   

免疫细胞化学法测定鱼类淋巴囊肿病毒(LCDV)滴度     

宋佳蕾  绳秀珍  战文斌 《中国海洋大学学报(自然科学版)》2012,42(5):55-59

利用免疫细胞化学法测定鱼类淋巴囊肿病毒(LCDV)滴度.以牙鲆鳃细胞系(FG)作为感染细胞,将生长旺盛的FG细胞接种于48孔培养板中培养至形成细胞单层,用2倍连续稀释的LCDV粗提液分别接种FG细胞.固定各稀释度LCDV感染后的FG细胞,孵育抗牙鲆LCDV单克隆抗体,其后再运用生物素-亲合素反应系统,以碱性磷酸酶底物APRed试剂盒发色.倒置显微镜观察,被病毒感染的FG细胞的细胞质呈现红色,未被感染细胞的细胞质呈无色.记录各稀释度病毒感染的阳性细胞孔数,按Reed-Muench法计算组织细胞培养半数感染量(TCID0).结果显示,免疫细胞化学法测得LCDV在FG的滴度为1.77×210 TCID50/mL.该法可以用来有效测定LCDV滴度,且结果直观、准确性较好,灵敏度较高.  相似文献   

一种基于交叉剖面的地质模型构建方法     

陈国良  刘修国  盛谦  张勇慧 《岩土力学》2011,32(8):2409-2415

针对基于近似平行断面数据的建模方法的缺点,利用交叉地质剖面数据,提出了一种通过划分空间单元格的思路来构建地质结构模型的方法。该方法基于“分而自治”的思想,将复杂的整体的建模区域划分为单个单元格内的建模,待每个格子内的建模完成之后,采用相反的思路,将所有格子内的模型拼接起来形成最终的建模区域内的整体模型  相似文献   

驼背鲈外周血细胞的形态学研究   总被引：1，自引：0，他引：1       下载免费PDF全文

王静香  李加儿  区又君 《海洋科学》2011,35(4):77-82

利用Wright's和Giemsa双重染色法和改良的血涂片染色法两种方式,通过光镜对驼背鲈(Cromileptes altivelis)的血涂片进行了观察.结果表明:驼背鲈的血涂片中可区分出6种细胞--红细胞、嗜中性粒细胞、嗜酸性粒细胞、单核细胞、淋巴细胞、血栓细胞,偶尔可见到分裂的红细胞、分裂的血栓细胞、核影红细胞和...  相似文献   

Biofilm Formation by Microorganisms Isolated from Paper Mill Effluent and its Public Health Implications     

Priyanka Lal  Vishnu Agarwal  Parul Pruthi  Swaranjit Singh Cameotra  Vikas Pruthi 《洁净——土壤、空气、水》2008,36(12):963-968

The microbiological assessment of biofilm formation from paper mill effluent discharged through a pipeline revealed a maximum microbial count for Pseudomonas (5·10⁶ cfu/mL) followed by Staphylococcus (4·10⁶ cfu/mL), Bacillus (8.2·10⁵ cfu/mL), Burkholderia (7.2·10⁵ cfu/mL), Enterobacter (5.3·10⁴ cfu/mL), Acinetobacter (4.1·10³ cfu/mL), Alcaligenes (1.2·10² cfu/mL) and Klebsiella (0.8·10² cfu/mL) species. Among these species, the maximum biofilm formation was observed after 24 h of incubation by Pseudomonas sp. using a crystal violet (CV) assay. This isolate was later identified by 16S rRNA amplification to be Pseudomonas aeruginosa PME1. Extracellular polymeric substances (EPS) of P. aeruginosa PME1 in the biofilm showed a reduction in total carbohydrate content (42%) with increased protein (9.0%), hexosamine (3.0%) and uronic acid (1.7%) content as compared to its planktonic form. Antimicrobial susceptibility testing revealed that P. aeruginosa PME1 biofilms were 17, 24, 27, 30, and 32 times more resistant to cefotaxime, imipenem, ceftazidime, tazobactam and piperacillin, respectively, than their free flowing counterparts.  相似文献   

On reproducing uniform flow exactly on general hexahedral cells using one degree of freedom per surface     

J.M. Nordbotten  H. Hægland 《Advances in water resources》2009

The solution of second order elliptic partial differential equations typically arising for flow problems, e.g., in porous media, is frequently expressed as face fluxes of a flow field at hexahedral cells. In this letter, we show by example that the following properties are incompatible: (1) A local velocity reconstruction, (2) reproduction of uniform flow for general hexahedral cells, and (3) a flow field in H(div). This is in particular relevant to mixed finite element methods approximating H(div), and to other methods trying to reconstruct a flow field in a hexahedron using only local face fluxes, e.g., streamline tracing methods for reservoir simulation.  相似文献   

鲎血细胞提取物生物活性的初步研究   总被引：7，自引：0，他引：7  

张海涛  梁景耀  翁云  祝其锋 《海洋科学》2000,24(10):48-50

采用酸抽提法和Ｓｅｐｈａｄｅｘ Ｇ－５０凝胶层析从鲎血细胞中分离出含有一低分子量组分的提取物,ＳＤＳ－ＰＡＧＥ显示一条区带。提取物可以抑制内毒素诱导的鲎试剂（ＴＡＬ）反应,抑制金色葡萄菌和大肠杆菌的生长,并发现２５ｍｇ／Ｌ的提取物对ＨＬ－６０细胞的生长有强烈的抑制作用。  相似文献