A culture-dependent survey of thermophilic bacteria from hot springs in Xiamen area in China     

YANG Bo  OUYANG Jianping  AO Jingqun  CHEN Xinhua 《海洋学报(英文版)》2009,28(1):62-71

Microbes are believed to play important roles in ecosystem function in many environments. The hot springs of Xiamen Island are close to the Xiamen Sea, and may have some characteristics different from those of inland hot springs. Microbes living in the hot springs of Xiamen may have new characteristics. However, little is known about microbial communities of hot springs close to the Xiamen Sea. A culturedependent survey of microbial population in the Xiamen hot springs was performed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing. A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs, including neutrophilic thermophilic bacteria and moderately thermophilic acidophiles. Neutrophilic thermophilic bacteria, which grow at a temperature range of 55—90℃ including Rhodothermus marinus (Strain 1), Thermus thermophilus (Strain 2), Thermus thiopara (Strain 3), Geobacillus stearothermophilus (Strain 4), Geobacillus thermoleovorans (Strain 5), and Pseudomonas pseudoalcaligenes (Strain 6), were recovered by 2216E plates. Moderately thermophilic acidophiles, which can grow at temperatures above 50℃ and a pH range of 1.8—3.5 such as Alicyclobacillus acidoterrestris (Strain 8), Sulfobacillus acidophilus (Strain 9), and Sulfobacillus thermosulfidooxidans (Strain 10), were isolated on selective solid medium containing sulfur and Fe2+. Among these strains, Rhodothermus marinus, Thermus thermophilus and Geobacillus stearothermophilus are not only thermophiles, but also halophiles. One bacterium strain (Strain 6) shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene sequence, but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics, suggesting that it may represent a novel thermophilic species. Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodothermus marinus and Thermus thermophilus dominated the cultivable microbial community.  相似文献   

用于养殖环境调控的微生物制剂评价方法的研究   总被引：2，自引：0，他引：2  

王雷  刘梅  王宝杰  蒋克勇  彭虹旎  万奎吉  万乃宝 《海洋科学》2009,33(5):6-10

为尝试建立对不同微生物制剂产品的客观评价方法体系,选择5种商品化复合微生物制剂,进行了菌数和主要组成种类等测定,并利用天然或人工模拟的养殖污水进行了不同微生物制剂对氨氮、亚硝酸态氮、COD等降解效果的比较,以及微生物制剂活化增效后的效果比较等.结果表明,不同的商业化复合微生物制剂在有益菌含量和水质污染降解效果方面存在较大差异.另外,微生物制剂在海水和淡水中以及是否经过活化,效果也存在较大差异.  相似文献   

Response of Microbial Communities in Soil to Multi-level Warming in a Highland Barley System of the Lhasa River     

FU Gang  SUN Wei  LI Shaowei  ZHONG Zhiming 《资源与生态学报(英文版)》2019,10(4):373-378

No studies have examined the effect of experimental warming on the microbial biomass and community composition of soil in agricultural ecosystem on the Qinghai-Tibet Plateau. Thus it is unclear whether the influences of experimental warming on microbial communities in soil are related to warming magnitude in croplands on this Plateau. This study performed warming experiment (control, low- and high-level) in a highland barley system of the Lhasa River in May 2014 to examine the correlation between the response of microbial communities in soil to warming and warming magnitude. Topsoil samples (0-10 and 10-20 cm) were collected on September 14, 2014. Experimental warming at both low and high levels significantly increased soil temperature by 1.02 ℃ and 1.59 ℃, respectively at the depth of 15 cm. Phospho lipid fatty acid (PLFA) method was used to determine the microbial community in soil. The low-level experimental warming did not significantly affect the soil’s total PLFA, fungi, bacteria, arbuscular mycorrhizal fungi (AMF), actinomycetes, gram-positive bacteria (G⁺), gram-negative bacteria (G^-), protozoa, the ratio of fungi to bacteria (F/B ratio), and ratio of G⁺ to G^- (G⁺/G^- ratio) at the 0-10 and 10-20 cm depth. The low-level experimental warming also did not significantly alter the composition of microbial community in soil at the 0-10 and 10-20 cm depth. The high-level experimental warming significantly increased total PLFA by 74.4%, fungi by 78.0%, bacteria by 74.0%, AMF by 66.9%, actinomycetes by 81.4%, G⁺ by 67.0% and G^- by 74.4% at the 0-10 cm depth rather than at 10-20 cm depth. The high-level experimental warming significantly altered microbial community composition in soil at the 0-10 cm depth rather than at 10-20 cm depth. Our findings suggest that the response of microbial communities in soil to warming varied with warming magnitudes in the highland barley system of the Lhasa River.  相似文献   

胶州湾沉积物中石油降解菌的降解能力及多样性研究     

高伟  高祥兴  米铁柱  韩彬  张毅然  李馨子  尹晓斐  孙承君  李倩  崔志松  栾晓  于志刚  郑立 《海洋学报(英文版)》2019,38(6):54-64

2013年青岛输油管道爆炸,大量石油污染了附近海岸。课题组采集了污染的沉积物样品,以原油为唯一碳源和能源,富集了四个石油降解菌群。生物多样性和群落分析表明,Luteibacter、Parvibaculum 和属于食烷菌科的一个属是降解菌群的主要优势菌,都属于变形菌门。从石油降解菌群中分离筛选,获得了9株具有不同16S rRNA基因序列的降解菌,分别属于8个属。重量法测定降解菌的石油降解率,其中5株的石油降解率大于30%。GC-MS分析结果表明,石油降解菌多倾向于降解烷烃,对多环芳烃的降解能力较差,其中5株细菌的烷烃降解率较大,仅1株菌D2对多环芳烃的降解率较大,其降解率在34.9%到77.5%。通过对高通量数据的分析,表明食烷菌属是菌群A和菌群E的主要降解菌群,其中筛选获得的菌株E4可能是菌群E的一株优势降解菌。本研究所筛选菌株证明了其石油降解潜力,为油污染海滩生物修复提供了菌株资源。  相似文献   

淡化浓海水日晒盐田原核微生物多样性分析     

秦乐宁  黄鑫洁  朱大玲  侍亚敏  唐娜  王学魁 《海洋科学》2019,43(5):27-35

海滨日晒盐田具有稳定且独特的生态系统。淡化浓海水排入盐田制盐,对盐田生态系统的稳定及盐田的可持续发展具有一定的影响。本研究分析天津汉沽淡化浓海水日晒盐田不同季节不同盐度盐池原核微生物多样性现状及群落结构变化,以期为评估淡化浓海水对盐田原核微生物群落影响提供理论依据。采用温度梯度凝胶电泳和序列测定等方法,分析不同月份不同盐度盐池中细菌和古菌的多样性与群落结构变化。分别采用香农-威纳指数、加权丰富度指数和均匀度指数分析盐田微生物群落多样性、丰富度和均匀度。结果表明,淡化浓海水和溴后水中均未检测出细菌和古菌。不同月份不同盐度盐池样本中细菌多样性变化不大,优势菌主要为γ-变形菌门(γ-Proteobacteria)中的Spiribacter salinus和Pseudoalteromonassp.;古菌仅出现在盐度最高的Ⅶ号盐池中,优势古菌为广古菌门(Euryarchaeota)的Halogeometricum sp.。天津汉沽淡化浓海水日晒盐田中细菌的多样性和丰富度较低,春夏两季细菌群落结构较为稳定,秋冬季节细菌群落结构变化明显。盐度不同的盐池中细菌群落结构变化较大,随着盐度逐渐升高, Spiribacter salinus逐渐取代Pseudoalteromonas sp.成为占主要地位的优势菌种。  相似文献   

Microbial diversity in the sediments of the southern Mariana Trench     

LI Yingjie  CAO Wenrui  WANG Yan  MA Qingjun 《海洋湖沼学报（英文）》2019,(3):1024-1029

Microbial diversity in the abyssal sediments beneath the seafloor of 30,94,and 151cm near the southern end of the Mariana Trench was analyzed in the Illumina HiSeq 2500 platform.Results show that the microbial populations were dominated by bacteria but merely no archaea were identifi ed at the three depths.In the bacterial community,Proteobacteria and Firmicutes dominated the total taxon tags,followed by Bacteroidetes,Actinobacteria,Planctomycetes,Cyanobacteria,and Chloroflexi,which together account for over 99%of the total population.Similar to that in the seawater in the trench,the operational taxonomic units(OTUs)belonging to Gammaproteobacteria from the sediment samples showed high abundance.However,common bacterial OTUs in the water of the trench including Nitrospirae and Marinimicrobia were hardly found in the sediments from the southern Mariana Trench or the hadal region.Therefore,this study documented for the first time the compositions of microbial diversity in the trench sediments,revealed the difference in microbial diversity in water and sediment of the trench and will enrich the knowledge on the microbial diversity in the abyssal areas.  相似文献   

Microbial diversity in Huguangyan Maar Lake of China revealed by high-throughput sequencing     

HOU Qinghua  FANG Zhou  ZHU Qingmei  DONG Hongpo 《海洋湖沼学报（英文）》2019,(4):1245-1257

Huguangyan Maar Lake is a typical maar lake in the southeast of China. It is well preserved and not disturbed by anthropogenic activities. In this study, microbial community structures in sediment and water samples from Huguangyan Maar Lake were investigated using a high-throughput sequencing method. We found significant differences between the microbial community compositions of the water and the sediment. The sediment samples contained more diverse Bacteria and Archaea than did the water samples. Actinobacteria, Betaproteobacteria, Cyanobacteria, and Deltaproteobacteria predominated in the water samples while Deltaproteobacteria, Anaerolineae, Nitrospira, and Dehalococcoidia were the major bacterial groups in the sediment. As for Archaea, Woesearchaeota (DHVEG-6), unclassified Archaea, and Deep Sea Euryarchaeotic Group were detected at higher abundances in the water, whereas the Miscellaneous Crenarchaeotic Group, Thermoplasmata, and Methanomicrobia were significantly more abundant in the sediment. Interactions between Bacteria and Archaea were common in both the water column and the sediment. The concentrations of major nutrients (NO^3-, PO4^3-, SiO3^2- and NH4^+) shaped the microbial population structures in the water. At the higher phylogenetic levels including phylum and class, many of the dominant groups were those that were also abundant in other lakes;however, novel microbial populations (unclassified) were often seen at the lower phylogenetic levels. Our study lays a foundation for examining microbial biogeochemical cycling in sequestered lakes or reservoirs.  相似文献   

Characteristics of bottom dissolved oxygen in Long Island Sound,New York     

Younjoo J. Lee  Kamazima M.M. Lwiza 《Estuarine, Coastal and Shelf Science》2008

The variability of bottom dissolved oxygen (DO) in Long Island Sound, New York, is examined using water quality monitoring data collected by the Connecticut Department of Environmental Protection from 1995 to 2004. Self-organizing map analysis indicates that hypoxia always occurs in the Narrows during summer and less frequently in the Western and the Central Basins. The primary factor controlling the bottom DO, changes spatially and temporally. For non-summer seasons, the levels of bottom DO are strongly associated with water temperature, which means DO availability is primarily driven by solubility. During summer, stratification intensifies under weak wind conditions and bottom DO starts to decrease and deviate from the saturation level except for stations in the Eastern Basin. For the westernmost and shallow (<15 m) stations, bottom DO is correlated with the density stratification (represented by difference between surface and bottom density). In contrast, at deep stations (>20 m), the relationship between oxygen depletion and stratification is not significant. For stations located west of the Central Basin, bottom DO continues to decrease during summer until it reaches its minimum when bottom temperature is around 19–20 °C. In most cases the recovery to saturation levels at the beginning of fall is fast, but not necessarily associated with increased wind mixing. Therefore, we propose that the DO recovery may be a manifestation of either the reduced microbial activity combined with the depletion of organic matter or horizontal exchange. Hypoxic volume is weakly correlated to the summer wind speed, spring total nitrogen, spring chlorophyll a, and maximum river discharge. When all variables are combined in a multiple regression, the coefficient of determination (r²) is 0.92. Surprisingly, the weakest variable is the total nitrogen, because when it is excluded the coefficient r² only drops to 0.84. Spring bloom seems to be an important source of organic carbon pool and biological uptake of oxygen plays a more crucial role in the seasonal evolution of bottom DO than previously thought. Our results indicate that the reassessment phase of the Long Island Sound Total Maximum Daily Load policy on nitrogen loading will most likely fail, because it ignores the contributions of the spring organic carbon pool and river discharge. Also, it is questionable whether the goal of 58.5% anthropogenic nitrogen load reduction is enough.  相似文献   

鲁奇管壳型合成塔合成燃料甲醇的数学模型     

贾明生 《广东海洋大学学报》1995,(2)

采用单速率模型建立了鲁奇（ｌｕｒｇｉ）管壳型合成塔合成燃料甲醇的数学模型，并对影响数学模型一些主要因素进行了模拟计算及讨论，得出了鲁奇管壳型合成塔适用于合成燃料甲醇的生产工艺条件。  相似文献   

Decomposition of root litter and related microbial population dynamics of a Negev Desert shrub, Zygophyllum dumosum     

Y. Steinberger  R. Degani  G. Barness 《Journal of Arid Environments》1995,31(4)

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